SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8BZN4

- NUAK2_MOUSE

UniProt

Q8BZN4 - NUAK2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
NUAK family SNF1-like kinase 2
Gene
Nuak2, Omphk2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-220 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851ATP By similarityBy similarity
Active sitei187 – 1871Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 719ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. apoptotic process Source: UniProtKB-KW
  3. cellular response to glucose starvation Source: UniProtKB
  4. negative regulation of apoptotic process Source: UniProtKB
  5. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NUAK family SNF1-like kinase 2 (EC:2.7.11.1)
Alternative name(s):
Omphalocele kinase 2
Gene namesi
Name:Nuak2
Synonyms:Omphk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1921387. Nuak2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639NUAK family SNF1-like kinase 2
PRO_0000247757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei220 – 2201Phosphothreonine By similarityBy similarity
Modified residuei538 – 5381Phosphoserine By similarity
Modified residuei559 – 5591Phosphoserine By similarity
Modified residuei562 – 5621Phosphoserine By similarity
Modified residuei588 – 5881Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated at Thr-220 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BZN4.
PRIDEiQ8BZN4.

PTM databases

PhosphoSiteiQ8BZN4.

Expressioni

Developmental stagei

First expressed in the anterior neuroectoderm at E7.5. At E8.5, the expression is present in future forebrain and midbrain regions. At E9.5, the expression is intense in the forebrain and midbrain with a sharp caudal boundary at the isthmic region; a low level of the expression is found throughout the neuroectoderm. Not expressed in the ventral body wall.1 Publication

Gene expression databases

BgeeiQ8BZN4.
CleanExiMM_NUAK2.
GenevestigatoriQ8BZN4.

Interactioni

Protein-protein interaction databases

BioGridi216519. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BZN4.
SMRiQ8BZN4. Positions 44-338.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 315259Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115114.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ8BZN4.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG7MD4PN.
PhylomeDBiQ8BZN4.
TreeFamiTF324572.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 12 Publications (identifier: Q8BZN4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK    50
HNLRHRYEFL ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH 100
IRREIEIMSS LNHPHIIAIH EVGRSRLVTV FENSSKIVIV MEYASRGDLY 150
DYISERPRLS ERDARHFFRQ IVSALHYCHQ NGIVHRDLKL ENILLDANGN 200
IKIADFGLSN LYHKGKFLQT FCGSPLYASP EIVNGKPYVG PEVDSWSLGV 250
LLYILVHGTM PFDGQDHKTL VKQISNGAYR EPPKPSDACG LIRWLLMVNP 300
TRRATLEDVA SHWWVNWGYT TGVGEQEALR EGGHPSGDFG RASMADWLRR 350
SSRPLLENGA KVCSFFKQHV PGGGSTVPGL ERQHSLKKSR KENDMAQNLQ 400
GDPAEDTSSR PGKSSLKLPK GILKKKSSTS SGEVQEDPQE LRPVPDTPGQ 450
PVPAVSLLPR KGILKKSRQR ESGYYSSPEP SESGELLDAS DVFVSGDPVE 500
QKSPQASGLL LHRKGILKLN GKFSRTALEG TTPSTFGSLD QLASSHPAAR 550
PSRPSGAVSE DSILSSESFD QLDLPERLPE TPLRGCVSVD NLRGLEQPPS 600
EGLKRWWQES LGDSCFSLTD CQEVTAAYRQ ALGICSKLS 639
Length:639
Mass (Da):70,675
Last modified:July 25, 2006 - v2
Checksum:iFB9C40228F53872C
GO
Isoform 22 Publications (identifier: Q8BZN4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-129: Missing.

Show »
Length:631
Mass (Da):69,806
Checksum:iD51C042DE6CC174B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei122 – 1298Missing in isoform 2. 2 Publications
VSP_052095

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41V → G in BAC28575. 1 Publication
Sequence conflicti339 – 3391F → S in AAH33302. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004737 mRNA. Translation: BAB23518.1.
AK033672 mRNA. Translation: BAC28421.1.
AK034082 mRNA. Translation: BAC28575.1.
BC033302 mRNA. Translation: AAH33302.1.
BC046833 mRNA. Translation: AAH46833.1.
CCDSiCCDS15283.1. [Q8BZN4-2]
CCDS56642.1. [Q8BZN4-1]
RefSeqiNP_001181954.1. NM_001195025.1. [Q8BZN4-1]
NP_083054.1. NM_028778.4. [Q8BZN4-2]
UniGeneiMm.100666.

Genome annotation databases

EnsembliENSMUST00000072177; ENSMUSP00000072039; ENSMUSG00000009772. [Q8BZN4-1]
ENSMUST00000082125; ENSMUSP00000080769; ENSMUSG00000009772. [Q8BZN4-2]
GeneIDi74137.
KEGGimmu:74137.
UCSCiuc007coo.2. mouse. [Q8BZN4-2]
uc007cop.2. mouse. [Q8BZN4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004737 mRNA. Translation: BAB23518.1 .
AK033672 mRNA. Translation: BAC28421.1 .
AK034082 mRNA. Translation: BAC28575.1 .
BC033302 mRNA. Translation: AAH33302.1 .
BC046833 mRNA. Translation: AAH46833.1 .
CCDSi CCDS15283.1. [Q8BZN4-2 ]
CCDS56642.1. [Q8BZN4-1 ]
RefSeqi NP_001181954.1. NM_001195025.1. [Q8BZN4-1 ]
NP_083054.1. NM_028778.4. [Q8BZN4-2 ]
UniGenei Mm.100666.

3D structure databases

ProteinModelPortali Q8BZN4.
SMRi Q8BZN4. Positions 44-338.
ModBasei Search...

Protein-protein interaction databases

BioGridi 216519. 4 interactions.

PTM databases

PhosphoSitei Q8BZN4.

Proteomic databases

PaxDbi Q8BZN4.
PRIDEi Q8BZN4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000072177 ; ENSMUSP00000072039 ; ENSMUSG00000009772 . [Q8BZN4-1 ]
ENSMUST00000082125 ; ENSMUSP00000080769 ; ENSMUSG00000009772 . [Q8BZN4-2 ]
GeneIDi 74137.
KEGGi mmu:74137.
UCSCi uc007coo.2. mouse. [Q8BZN4-2 ]
uc007cop.2. mouse. [Q8BZN4-1 ]

Organism-specific databases

CTDi 81788.
MGIi MGI:1921387. Nuak2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115114.
HOGENOMi HOG000004864.
HOVERGENi HBG007160.
InParanoidi Q8BZN4.
KOi K08800.
OMAi TDCQEVT.
OrthoDBi EOG7MD4PN.
PhylomeDBi Q8BZN4.
TreeFami TF324572.

Miscellaneous databases

ChiTaRSi NUAK2. mouse.
NextBioi 339878.
PROi Q8BZN4.
SOURCEi Search...

Gene expression databases

Bgeei Q8BZN4.
CleanExi MM_NUAK2.
Genevestigatori Q8BZN4.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cecum, Diencephalon and Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and Czech II.
    Tissue: Brain and Mammary gland.
  3. "A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation."
    Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S.
    Dev. Dyn. 235:2229-2237(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNUAK2_MOUSE
AccessioniPrimary (citable) accession number: Q8BZN4
Secondary accession number(s): Q80ZW3, Q8CIC0, Q9DBV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi