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Q8BZN4

- NUAK2_MOUSE

UniProt

Q8BZN4 - NUAK2_MOUSE

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Protein

NUAK family SNF1-like kinase 2

Gene

Nuak2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-220.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851ATPBy similarityPROSITE-ProRule annotation
Active sitei187 – 1871Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 719ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. apoptotic process Source: UniProtKB-KW
  3. cellular response to glucose starvation Source: UniProtKB
  4. negative regulation of apoptotic process Source: UniProtKB
  5. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NUAK family SNF1-like kinase 2 (EC:2.7.11.1)
Alternative name(s):
Omphalocele kinase 2
Gene namesi
Name:Nuak2Imported
Synonyms:Omphk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1921387. Nuak2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639NUAK family SNF1-like kinase 2PRO_0000247757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei220 – 2201PhosphothreonineBy similarity
Modified residuei538 – 5381PhosphoserineBy similarity
Modified residuei559 – 5591PhosphoserineBy similarity
Modified residuei562 – 5621PhosphoserineBy similarity
Modified residuei588 – 5881Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated at Thr-220 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BZN4.
PaxDbiQ8BZN4.
PRIDEiQ8BZN4.

PTM databases

PhosphoSiteiQ8BZN4.

Expressioni

Developmental stagei

First expressed in the anterior neuroectoderm at E7.5. At E8.5, the expression is present in future forebrain and midbrain regions. At E9.5, the expression is intense in the forebrain and midbrain with a sharp caudal boundary at the isthmic region; a low level of the expression is found throughout the neuroectoderm. Not expressed in the ventral body wall.1 Publication

Gene expression databases

BgeeiQ8BZN4.
CleanExiMM_NUAK2.
GenevestigatoriQ8BZN4.

Interactioni

Protein-protein interaction databases

BioGridi216519. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BZN4.
SMRiQ8BZN4. Positions 44-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 315259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119334.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ8BZN4.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG7MD4PN.
PhylomeDBiQ8BZN4.
TreeFamiTF324572.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 12 Publications (identifier: Q8BZN4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK
60 70 80 90 100
HNLRHRYEFL ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH
110 120 130 140 150
IRREIEIMSS LNHPHIIAIH EVGRSRLVTV FENSSKIVIV MEYASRGDLY
160 170 180 190 200
DYISERPRLS ERDARHFFRQ IVSALHYCHQ NGIVHRDLKL ENILLDANGN
210 220 230 240 250
IKIADFGLSN LYHKGKFLQT FCGSPLYASP EIVNGKPYVG PEVDSWSLGV
260 270 280 290 300
LLYILVHGTM PFDGQDHKTL VKQISNGAYR EPPKPSDACG LIRWLLMVNP
310 320 330 340 350
TRRATLEDVA SHWWVNWGYT TGVGEQEALR EGGHPSGDFG RASMADWLRR
360 370 380 390 400
SSRPLLENGA KVCSFFKQHV PGGGSTVPGL ERQHSLKKSR KENDMAQNLQ
410 420 430 440 450
GDPAEDTSSR PGKSSLKLPK GILKKKSSTS SGEVQEDPQE LRPVPDTPGQ
460 470 480 490 500
PVPAVSLLPR KGILKKSRQR ESGYYSSPEP SESGELLDAS DVFVSGDPVE
510 520 530 540 550
QKSPQASGLL LHRKGILKLN GKFSRTALEG TTPSTFGSLD QLASSHPAAR
560 570 580 590 600
PSRPSGAVSE DSILSSESFD QLDLPERLPE TPLRGCVSVD NLRGLEQPPS
610 620 630
EGLKRWWQES LGDSCFSLTD CQEVTAAYRQ ALGICSKLS
Length:639
Mass (Da):70,675
Last modified:July 25, 2006 - v2
Checksum:iFB9C40228F53872C
GO
Isoform 22 Publications (identifier: Q8BZN4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-129: Missing.

Show »
Length:631
Mass (Da):69,806
Checksum:iD51C042DE6CC174B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41V → G in BAC28575. (PubMed:16141072)Curated
Sequence conflicti339 – 3391F → S in AAH33302. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei122 – 1298Missing in isoform 2. 2 PublicationsVSP_052095

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004737 mRNA. Translation: BAB23518.1.
AK033672 mRNA. Translation: BAC28421.1.
AK034082 mRNA. Translation: BAC28575.1.
BC033302 mRNA. Translation: AAH33302.1.
BC046833 mRNA. Translation: AAH46833.1.
CCDSiCCDS15283.1. [Q8BZN4-2]
CCDS56642.1. [Q8BZN4-1]
RefSeqiNP_001181954.1. NM_001195025.1. [Q8BZN4-1]
NP_083054.1. NM_028778.4. [Q8BZN4-2]
UniGeneiMm.100666.

Genome annotation databases

EnsembliENSMUST00000072177; ENSMUSP00000072039; ENSMUSG00000009772. [Q8BZN4-1]
ENSMUST00000082125; ENSMUSP00000080769; ENSMUSG00000009772. [Q8BZN4-2]
GeneIDi74137.
KEGGimmu:74137.
UCSCiuc007coo.2. mouse. [Q8BZN4-2]
uc007cop.2. mouse. [Q8BZN4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004737 mRNA. Translation: BAB23518.1 .
AK033672 mRNA. Translation: BAC28421.1 .
AK034082 mRNA. Translation: BAC28575.1 .
BC033302 mRNA. Translation: AAH33302.1 .
BC046833 mRNA. Translation: AAH46833.1 .
CCDSi CCDS15283.1. [Q8BZN4-2 ]
CCDS56642.1. [Q8BZN4-1 ]
RefSeqi NP_001181954.1. NM_001195025.1. [Q8BZN4-1 ]
NP_083054.1. NM_028778.4. [Q8BZN4-2 ]
UniGenei Mm.100666.

3D structure databases

ProteinModelPortali Q8BZN4.
SMRi Q8BZN4. Positions 44-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 216519. 4 interactions.

PTM databases

PhosphoSitei Q8BZN4.

Proteomic databases

MaxQBi Q8BZN4.
PaxDbi Q8BZN4.
PRIDEi Q8BZN4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000072177 ; ENSMUSP00000072039 ; ENSMUSG00000009772 . [Q8BZN4-1 ]
ENSMUST00000082125 ; ENSMUSP00000080769 ; ENSMUSG00000009772 . [Q8BZN4-2 ]
GeneIDi 74137.
KEGGi mmu:74137.
UCSCi uc007coo.2. mouse. [Q8BZN4-2 ]
uc007cop.2. mouse. [Q8BZN4-1 ]

Organism-specific databases

CTDi 81788.
MGIi MGI:1921387. Nuak2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119334.
HOGENOMi HOG000004864.
HOVERGENi HBG007160.
InParanoidi Q8BZN4.
KOi K08800.
OMAi TDCQEVT.
OrthoDBi EOG7MD4PN.
PhylomeDBi Q8BZN4.
TreeFami TF324572.

Miscellaneous databases

ChiTaRSi NUAK2. mouse.
NextBioi 339878.
PROi Q8BZN4.
SOURCEi Search...

Gene expression databases

Bgeei Q8BZN4.
CleanExi MM_NUAK2.
Genevestigatori Q8BZN4.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6JImported.
    Tissue: CecumImported, DiencephalonImported and LungImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6Imported and Czech IIImported.
    Tissue: BrainImported and Mammary glandImported.
  3. "A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation."
    Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S.
    Dev. Dyn. 235:2229-2237(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNUAK2_MOUSE
AccessioniPrimary (citable) accession number: Q8BZN4
Secondary accession number(s): Q80ZW3, Q8CIC0, Q9DBV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3