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Q8BZN4 (NUAK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NUAK family SNF1-like kinase 2

EC=2.7.11.1
Alternative name(s):
Omphalocele kinase 2
Gene names
Name:Nuak2
Synonyms:Omphk2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 By similarity. UniProtKB Q9H093

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9H093

Cofactor

Magnesium By similarity. UniProtKB Q9H093

Enzyme regulation

Activated by phosphorylation on Thr-220 By similarity.

Developmental stage

First expressed in the anterior neuroectoderm at E7.5. At E8.5, the expression is present in future forebrain and midbrain regions. At E9.5, the expression is intense in the forebrain and midbrain with a sharp caudal boundary at the isthmic region; a low level of the expression is found throughout the neuroectoderm. Not expressed in the ventral body wall. Ref.3

Post-translational modification

Phosphorylated at Thr-220 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.2 (identifier: Q8BZN4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 Ref.2 (identifier: Q8BZN4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-129: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 639639NUAK family SNF1-like kinase 2
PRO_0000247757

Regions

Domain57 – 315259Protein kinase
Nucleotide binding63 – 719ATP By similarity UniProtKB O60285

Sites

Active site1871Proton acceptor By similarity UniProtKB O60285
Binding site851ATP By similarity UniProtKB Q9H093

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue2201Phosphothreonine By similarity UniProtKB Q9H093
Modified residue5381Phosphoserine By similarity
Modified residue5591Phosphoserine By similarity
Modified residue5621Phosphoserine By similarity
Modified residue5881Phosphoserine Ref.4

Natural variations

Alternative sequence122 – 1298Missing in isoform 2. Ref.1 Ref.2
VSP_052095

Experimental info

Sequence conflict41V → G in BAC28575. Ref.1
Sequence conflict3391F → S in AAH33302. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: FB9C40228F53872C

FASTA63970,675
        10         20         30         40         50         60 
MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK HNLRHRYEFL 

        70         80         90        100        110        120 
ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH IRREIEIMSS LNHPHIIAIH 

       130        140        150        160        170        180 
EVGRSRLVTV FENSSKIVIV MEYASRGDLY DYISERPRLS ERDARHFFRQ IVSALHYCHQ 

       190        200        210        220        230        240 
NGIVHRDLKL ENILLDANGN IKIADFGLSN LYHKGKFLQT FCGSPLYASP EIVNGKPYVG 

       250        260        270        280        290        300 
PEVDSWSLGV LLYILVHGTM PFDGQDHKTL VKQISNGAYR EPPKPSDACG LIRWLLMVNP 

       310        320        330        340        350        360 
TRRATLEDVA SHWWVNWGYT TGVGEQEALR EGGHPSGDFG RASMADWLRR SSRPLLENGA 

       370        380        390        400        410        420 
KVCSFFKQHV PGGGSTVPGL ERQHSLKKSR KENDMAQNLQ GDPAEDTSSR PGKSSLKLPK 

       430        440        450        460        470        480 
GILKKKSSTS SGEVQEDPQE LRPVPDTPGQ PVPAVSLLPR KGILKKSRQR ESGYYSSPEP 

       490        500        510        520        530        540 
SESGELLDAS DVFVSGDPVE QKSPQASGLL LHRKGILKLN GKFSRTALEG TTPSTFGSLD 

       550        560        570        580        590        600 
QLASSHPAAR PSRPSGAVSE DSILSSESFD QLDLPERLPE TPLRGCVSVD NLRGLEQPPS 

       610        620        630 
EGLKRWWQES LGDSCFSLTD CQEVTAAYRQ ALGICSKLS 

« Hide

Isoform 2 [UniParc].

Checksum: D51C042DE6CC174B
Show »

FASTA63169,806

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Cecum, Diencephalon and Lung.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and Czech II.
Tissue: Brain and Mammary gland.
[3]"A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation."
Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S.
Dev. Dyn. 235:2229-2237(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004737 mRNA. Translation: BAB23518.1.
AK033672 mRNA. Translation: BAC28421.1.
AK034082 mRNA. Translation: BAC28575.1.
BC033302 mRNA. Translation: AAH33302.1.
BC046833 mRNA. Translation: AAH46833.1.
RefSeqNP_001181954.1. NM_001195025.1.
NP_083054.1. NM_028778.4.
UniGeneMm.100666.

3D structure databases

ProteinModelPortalQ8BZN4.
SMRQ8BZN4. Positions 44-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid216519. 4 interactions.

PTM databases

PhosphoSiteQ8BZN4.

Proteomic databases

PaxDbQ8BZN4.
PRIDEQ8BZN4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072177; ENSMUSP00000072039; ENSMUSG00000009772. [Q8BZN4-1]
ENSMUST00000082125; ENSMUSP00000080769; ENSMUSG00000009772. [Q8BZN4-2]
GeneID74137.
KEGGmmu:74137.
UCSCuc007coo.2. mouse. [Q8BZN4-2]
uc007cop.2. mouse. [Q8BZN4-1]

Organism-specific databases

CTD81788.
MGIMGI:1921387. Nuak2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000115114.
HOGENOMHOG000004864.
HOVERGENHBG007160.
InParanoidQ8BZN4.
KOK08800.
OMATDCQEVT.
OrthoDBEOG7MD4PN.
PhylomeDBQ8BZN4.
TreeFamTF324572.

Gene expression databases

BgeeQ8BZN4.
CleanExMM_NUAK2.
GenevestigatorQ8BZN4.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUAK2. mouse.
NextBio339878.
PROQ8BZN4.
SOURCESearch...

Entry information

Entry nameNUAK2_MOUSE
AccessionPrimary (citable) accession number: Q8BZN4
Secondary accession number(s): Q80ZW3, Q8CIC0, Q9DBV0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot