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Q8BZN4

- NUAK2_MOUSE

UniProt

Q8BZN4 - NUAK2_MOUSE

Protein

NUAK family SNF1-like kinase 2

Gene

Nuak2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by phosphorylation on Thr-220.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851ATPBy similarityPROSITE-ProRule annotation
    Active sitei187 – 1871Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi63 – 719ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. apoptotic process Source: UniProtKB-KW
    3. cellular response to glucose starvation Source: UniProtKB
    4. negative regulation of apoptotic process Source: UniProtKB
    5. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NUAK family SNF1-like kinase 2 (EC:2.7.11.1)
    Alternative name(s):
    Omphalocele kinase 2
    Gene namesi
    Name:Nuak2Imported
    Synonyms:Omphk2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1921387. Nuak2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 639639NUAK family SNF1-like kinase 2PRO_0000247757Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei220 – 2201PhosphothreonineBy similarity
    Modified residuei538 – 5381PhosphoserineBy similarity
    Modified residuei559 – 5591PhosphoserineBy similarity
    Modified residuei562 – 5621PhosphoserineBy similarity
    Modified residuei588 – 5881Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated at Thr-220 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BZN4.
    PRIDEiQ8BZN4.

    PTM databases

    PhosphoSiteiQ8BZN4.

    Expressioni

    Developmental stagei

    First expressed in the anterior neuroectoderm at E7.5. At E8.5, the expression is present in future forebrain and midbrain regions. At E9.5, the expression is intense in the forebrain and midbrain with a sharp caudal boundary at the isthmic region; a low level of the expression is found throughout the neuroectoderm. Not expressed in the ventral body wall.1 Publication

    Gene expression databases

    BgeeiQ8BZN4.
    CleanExiMM_NUAK2.
    GenevestigatoriQ8BZN4.

    Interactioni

    Protein-protein interaction databases

    BioGridi216519. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BZN4.
    SMRiQ8BZN4. Positions 44-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini57 – 315259Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115114.
    HOGENOMiHOG000004864.
    HOVERGENiHBG007160.
    InParanoidiQ8BZN4.
    KOiK08800.
    OMAiTDCQEVT.
    OrthoDBiEOG7MD4PN.
    PhylomeDBiQ8BZN4.
    TreeFamiTF324572.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 12 Publications (identifier: Q8BZN4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK    50
    HNLRHRYEFL ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH 100
    IRREIEIMSS LNHPHIIAIH EVGRSRLVTV FENSSKIVIV MEYASRGDLY 150
    DYISERPRLS ERDARHFFRQ IVSALHYCHQ NGIVHRDLKL ENILLDANGN 200
    IKIADFGLSN LYHKGKFLQT FCGSPLYASP EIVNGKPYVG PEVDSWSLGV 250
    LLYILVHGTM PFDGQDHKTL VKQISNGAYR EPPKPSDACG LIRWLLMVNP 300
    TRRATLEDVA SHWWVNWGYT TGVGEQEALR EGGHPSGDFG RASMADWLRR 350
    SSRPLLENGA KVCSFFKQHV PGGGSTVPGL ERQHSLKKSR KENDMAQNLQ 400
    GDPAEDTSSR PGKSSLKLPK GILKKKSSTS SGEVQEDPQE LRPVPDTPGQ 450
    PVPAVSLLPR KGILKKSRQR ESGYYSSPEP SESGELLDAS DVFVSGDPVE 500
    QKSPQASGLL LHRKGILKLN GKFSRTALEG TTPSTFGSLD QLASSHPAAR 550
    PSRPSGAVSE DSILSSESFD QLDLPERLPE TPLRGCVSVD NLRGLEQPPS 600
    EGLKRWWQES LGDSCFSLTD CQEVTAAYRQ ALGICSKLS 639
    Length:639
    Mass (Da):70,675
    Last modified:July 25, 2006 - v2
    Checksum:iFB9C40228F53872C
    GO
    Isoform 22 Publications (identifier: Q8BZN4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         122-129: Missing.

    Show »
    Length:631
    Mass (Da):69,806
    Checksum:iD51C042DE6CC174B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41V → G in BAC28575. (PubMed:16141072)Curated
    Sequence conflicti339 – 3391F → S in AAH33302. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei122 – 1298Missing in isoform 2. 2 PublicationsVSP_052095

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004737 mRNA. Translation: BAB23518.1.
    AK033672 mRNA. Translation: BAC28421.1.
    AK034082 mRNA. Translation: BAC28575.1.
    BC033302 mRNA. Translation: AAH33302.1.
    BC046833 mRNA. Translation: AAH46833.1.
    CCDSiCCDS15283.1. [Q8BZN4-2]
    CCDS56642.1. [Q8BZN4-1]
    RefSeqiNP_001181954.1. NM_001195025.1. [Q8BZN4-1]
    NP_083054.1. NM_028778.4. [Q8BZN4-2]
    UniGeneiMm.100666.

    Genome annotation databases

    EnsembliENSMUST00000072177; ENSMUSP00000072039; ENSMUSG00000009772. [Q8BZN4-1]
    ENSMUST00000082125; ENSMUSP00000080769; ENSMUSG00000009772. [Q8BZN4-2]
    GeneIDi74137.
    KEGGimmu:74137.
    UCSCiuc007coo.2. mouse. [Q8BZN4-2]
    uc007cop.2. mouse. [Q8BZN4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004737 mRNA. Translation: BAB23518.1 .
    AK033672 mRNA. Translation: BAC28421.1 .
    AK034082 mRNA. Translation: BAC28575.1 .
    BC033302 mRNA. Translation: AAH33302.1 .
    BC046833 mRNA. Translation: AAH46833.1 .
    CCDSi CCDS15283.1. [Q8BZN4-2 ]
    CCDS56642.1. [Q8BZN4-1 ]
    RefSeqi NP_001181954.1. NM_001195025.1. [Q8BZN4-1 ]
    NP_083054.1. NM_028778.4. [Q8BZN4-2 ]
    UniGenei Mm.100666.

    3D structure databases

    ProteinModelPortali Q8BZN4.
    SMRi Q8BZN4. Positions 44-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216519. 4 interactions.

    PTM databases

    PhosphoSitei Q8BZN4.

    Proteomic databases

    PaxDbi Q8BZN4.
    PRIDEi Q8BZN4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072177 ; ENSMUSP00000072039 ; ENSMUSG00000009772 . [Q8BZN4-1 ]
    ENSMUST00000082125 ; ENSMUSP00000080769 ; ENSMUSG00000009772 . [Q8BZN4-2 ]
    GeneIDi 74137.
    KEGGi mmu:74137.
    UCSCi uc007coo.2. mouse. [Q8BZN4-2 ]
    uc007cop.2. mouse. [Q8BZN4-1 ]

    Organism-specific databases

    CTDi 81788.
    MGIi MGI:1921387. Nuak2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115114.
    HOGENOMi HOG000004864.
    HOVERGENi HBG007160.
    InParanoidi Q8BZN4.
    KOi K08800.
    OMAi TDCQEVT.
    OrthoDBi EOG7MD4PN.
    PhylomeDBi Q8BZN4.
    TreeFami TF324572.

    Miscellaneous databases

    ChiTaRSi NUAK2. mouse.
    NextBioi 339878.
    PROi Q8BZN4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BZN4.
    CleanExi MM_NUAK2.
    Genevestigatori Q8BZN4.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6JImported.
      Tissue: CecumImported, DiencephalonImported and LungImported.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6Imported and Czech IIImported.
      Tissue: BrainImported and Mammary glandImported.
    3. "A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation."
      Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S.
      Dev. Dyn. 235:2229-2237(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNUAK2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BZN4
    Secondary accession number(s): Q80ZW3, Q8CIC0, Q9DBV0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3