Q8BZN4 (NUAK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NUAK family SNF1-like kinase 2
EC=2.7.11.1

Alternative name(s):
Omphalocele kinase 2

Gene names

Name:	Nuak2
Synonyms:	Omphk2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 By similarity. UniProtKB Q9H093
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9H093
Cofactor	Magnesium By similarity. UniProtKB Q9H093
Enzyme regulation	Activated by phosphorylation on Thr-220 By similarity.
Developmental stage	First expressed in the anterior neuroectoderm at E7.5. At E8.5, the expression is present in future forebrain and midbrain regions. At E9.5, the expression is intense in the forebrain and midbrain with a sharp caudal boundary at the isthmic region; a low level of the expression is found throughout the neuroectoderm. Not expressed in the ventral body wall. Ref.3
Post-translational modification	Phosphorylated at Thr-220 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cellular response to glucose starvation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.2 (identifier: Q8BZN4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 Ref.2 (identifier: Q8BZN4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-129: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	NUAK family SNF1-like kinase 2		PRO_0000247757
Regions
	Domain	57 – 315	259	Protein kinase
	Nucleotide binding	63 – 71	9	ATP By similarity UniProtKB O60285
Sites
	Active site	187	1	Proton acceptor By similarity UniProtKB O60285
	Binding site	85	1	ATP By similarity UniProtKB Q9H093
Amino acid modifications
	Modified residue	220	1	Phosphothreonine By similarity UniProtKB Q9H093
	Modified residue	538	1	Phosphoserine By similarity
	Modified residue	559	1	Phosphoserine By similarity
	Modified residue	562	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	122 – 129	8	Missing in isoform 2. Ref.1 Ref.2		VSP_052095
Experimental info
	Sequence conflict	4	1	V → G in BAC28575. Ref.1
	Sequence conflict	339	1	F → S in AAH33302. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: FB9C40228F53872C	FASTA	639	70,675
	10 20 30 40 50 60 MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK HNLRHRYEFL 70 80 90 100 110 120 ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH IRREIEIMSS LNHPHIIAIH 130 140 150 160 170 180 EVGRSRLVTV FENSSKIVIV MEYASRGDLY DYISERPRLS ERDARHFFRQ IVSALHYCHQ 190 200 210 220 230 240 NGIVHRDLKL ENILLDANGN IKIADFGLSN LYHKGKFLQT FCGSPLYASP EIVNGKPYVG 250 260 270 280 290 300 PEVDSWSLGV LLYILVHGTM PFDGQDHKTL VKQISNGAYR EPPKPSDACG LIRWLLMVNP 310 320 330 340 350 360 TRRATLEDVA SHWWVNWGYT TGVGEQEALR EGGHPSGDFG RASMADWLRR SSRPLLENGA 370 380 390 400 410 420 KVCSFFKQHV PGGGSTVPGL ERQHSLKKSR KENDMAQNLQ GDPAEDTSSR PGKSSLKLPK 430 440 450 460 470 480 GILKKKSSTS SGEVQEDPQE LRPVPDTPGQ PVPAVSLLPR KGILKKSRQR ESGYYSSPEP 490 500 510 520 530 540 SESGELLDAS DVFVSGDPVE QKSPQASGLL LHRKGILKLN GKFSRTALEG TTPSTFGSLD 550 560 570 580 590 600 QLASSHPAAR PSRPSGAVSE DSILSSESFD QLDLPERLPE TPLRGCVSVD NLRGLEQPPS 610 620 630 EGLKRWWQES LGDSCFSLTD CQEVTAAYRQ ALGICSKLS
	Isoform 2 [UniParc]. Checksum: D51C042DE6CC174B	FASTA	631	69,806
	10 20 30 40 50 60 MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK HNLRHRYEFL 70 80 90 100 110 120 ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH IRREIEIMSS LNHPHIIAIH 130 140 150 160 170 180 EVFENSSKIV IVMEYASRGD LYDYISERPR LSERDARHFF RQIVSALHYC HQNGIVHRDL 190 200 210 220 230 240 KLENILLDAN GNIKIADFGL SNLYHKGKFL QTFCGSPLYA SPEIVNGKPY VGPEVDSWSL 250 260 270 280 290 300 GVLLYILVHG TMPFDGQDHK TLVKQISNGA YREPPKPSDA CGLIRWLLMV NPTRRATLED 310 320 330 340 350 360 VASHWWVNWG YTTGVGEQEA LREGGHPSGD FGRASMADWL RRSSRPLLEN GAKVCSFFKQ 370 380 390 400 410 420 HVPGGGSTVP GLERQHSLKK SRKENDMAQN LQGDPAEDTS SRPGKSSLKL PKGILKKKSS 430 440 450 460 470 480 TSSGEVQEDP QELRPVPDTP GQPVPAVSLL PRKGILKKSR QRESGYYSSP EPSESGELLD 490 500 510 520 530 540 ASDVFVSGDP VEQKSPQASG LLLHRKGILK LNGKFSRTAL EGTTPSTFGS LDQLASSHPA 550 560 570 580 590 600 ARPSRPSGAV SEDSILSSES FDQLDLPERL PETPLRGCVS VDNLRGLEQP PSEGLKRWWQ 610 620 630 ESLGDSCFSL TDCQEVTAAY RQALGICSKL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cecum, Diencephalon and Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and Czech II. Tissue: Brain and Mammary gland.
[3]	"A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation." Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S. Dev. Dyn. 235:2229-2237(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004737 mRNA. Translation: BAB23518.1. AK033672 mRNA. Translation: BAC28421.1. AK034082 mRNA. Translation: BAC28575.1. BC033302 mRNA. Translation: AAH33302.1. BC046833 mRNA. Translation: AAH46833.1.
IPI	IPI00120164. IPI00404044.
RefSeq	NP_001181954.1. NM_001195025.1. NP_083054.1. NM_028778.4.
UniGene	Mm.100666.
3D structure databases
ProteinModelPortal	Q8BZN4.
SMR	Q8BZN4. Positions 44-319.
ModBase	Search...
PTM databases
PhosphoSite	Q8BZN4.
Proteomic databases
PaxDb	Q8BZN4.
PRIDE	Q8BZN4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000072177; ENSMUSP00000072039; ENSMUSG00000009772. ENSMUST00000082125; ENSMUSP00000080769; ENSMUSG00000009772.
GeneID	74137.
KEGG	mmu:74137.
UCSC	uc007coo.2. mouse. uc007cop.2. mouse.
Organism-specific databases
CTD	81788.
MGI	MGI:1921387. Nuak2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104353.
HOGENOM	HOG000004864.
HOVERGEN	HBG007160.
InParanoid	Q8BZN4.
KO	K08800.
OMA	TDCQEVT.
OrthoDB	EOG4MPHQ1.
Gene expression databases
Bgee	Q8BZN4.
CleanEx	MM_NUAK2.
Genevestigator	Q8BZN4.
GermOnline	ENSMUSG00000009772. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NUAK2. mouse.
NextBio	339878.
SOURCE	Search...

Entry information

Entry name

NUAK2_MOUSE

Accession

Primary (citable) accession number: Q8BZN4
Secondary accession number(s): Q80ZW3, Q8CIC0, Q9DBV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program