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Protein

Sialidase-4

Gene

Neu4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids (By similarity).By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateBy similarity
Binding sitei43 – 431SubstrateBy similarity
Active sitei47 – 471Proton acceptorBy similarity
Active sitei48 – 481Proton acceptorBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei222 – 2221SubstrateBy similarity
Binding sitei238 – 2381SubstrateBy similarity
Binding sitei383 – 3831SubstrateBy similarity
Active sitei413 – 4131NucleophileBy similarity
Active sitei434 – 4341Sequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_310546. Sialic acid metabolism.
REACT_339900. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-4 (EC:3.2.1.18)
Alternative name(s):
N-acetyl-alpha-neuraminidase 4
Neuraminidase 4
Gene namesi
Name:Neu4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2661364. Neu4.

Subcellular locationi

GO - Cellular componenti

  • lysosome Source: UniProtKB
  • mitochondrion Source: MGI
  • organelle inner membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Sialidase-4PRO_0000208907Add
BLAST

Proteomic databases

PaxDbiQ8BZL1.
PRIDEiQ8BZL1.

Expressioni

Tissue specificityi

Expressed predominantly in brain.1 Publication

Gene expression databases

BgeeiQ8BZL1.
CleanExiMM_NEU4.
ExpressionAtlasiQ8BZL1. baseline.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051151.

Structurei

3D structure databases

ProteinModelPortaliQ8BZL1.
SMRiQ8BZL1. Positions 22-284, 367-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati127 – 13812BNR 1Add
BLAST
Repeati200 – 21112BNR 2Add
BLAST
Repeati247 – 25812BNR 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 254FRIP motif

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 3 BNR repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG84455.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ8BZL1.
KOiK12357.
OMAiHIDCKEC.
OrthoDBiEOG7MSMNP.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR026946. Sialidase-4.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF8. PTHR10628:SF8. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZL1-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPTRVPRRT VLFQRERTGL TYRVPALLCV PPRPTLLAFA EQRLSPDDSH
60 70 80 90 100
AHRLVLRRGT LTRGSVRWGT LSVLETAVLE EHRSMNPCPV LDEHSGTIFL
110 120 130 140 150
FFIAVLGHTP EAVQIATGKN AARLCCVTSC DAGLTWGSVR DLTEEAIGAA
160 170 180 190 200
LQDWATFAVG PGHGVQLRSG RLLVPAYTYH VDRRECFGKI CWTSPHSLAF
210 220 230 240 250
YSDDHGISWH CGGLVPNLRS GECQLAAVDG DFLYCNARSP LGNRVQALSA
260 270 280 290 300
DEGTSFLPGE LVPTLAETAR GCQGSIVGFL APPSIEPQDD RWTGSPRNTP
310 320 330 340 350
HSPCFNLRVQ ESSGEGARGL LERWMPRLPL CYPQSRSPEN HGLEPGSDGD
360 370 380 390 400
KTSWTPECPM SSDSMLQSPT WLLYSHPAGR RARLHMGIYL SRSPLDPHSW
410 420 430 440 450
TEPWVIYEGP SGYSDLAFLG PMPGASLVFA CLFESGTRTS YEDISFCLFS
460 470
LADVLENVPT GLEMLSLRDK AQGHCWPS
Length:478
Mass (Da):52,426
Last modified:June 16, 2009 - v3
Checksum:iE1833F5DBFBFD08F
GO
Isoform 2 (identifier: Q8BZL1-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → METAGAPFCFHVDSLVPCSYWKVM

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):54,996
Checksum:i9CF4B78F19DF3BC2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → METAGAPFCFHVDSLVPCSY WKVM in isoform 2. 1 PublicationVSP_037492

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY258421 mRNA. Translation: AAP81169.1.
AK034236 mRNA. Translation: BAC28641.1.
BC070438 mRNA. Translation: AAH70438.1.
CCDSiCCDS15199.1. [Q8BZL1-2]
RefSeqiNP_776133.1. NM_173772.3. [Q8BZL1-2]
XP_006529656.1. XM_006529593.1. [Q8BZL1-1]
UniGeneiMm.214565.

Genome annotation databases

EnsembliENSMUST00000050890; ENSMUSP00000051151; ENSMUSG00000034000. [Q8BZL1-2]
ENSMUST00000190212; ENSMUSP00000140127; ENSMUSG00000034000. [Q8BZL1-1]
GeneIDi241159.
KEGGimmu:241159.
UCSCiuc007ceu.1. mouse. [Q8BZL1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY258421 mRNA. Translation: AAP81169.1.
AK034236 mRNA. Translation: BAC28641.1.
BC070438 mRNA. Translation: AAH70438.1.
CCDSiCCDS15199.1. [Q8BZL1-2]
RefSeqiNP_776133.1. NM_173772.3. [Q8BZL1-2]
XP_006529656.1. XM_006529593.1. [Q8BZL1-1]
UniGeneiMm.214565.

3D structure databases

ProteinModelPortaliQ8BZL1.
SMRiQ8BZL1. Positions 22-284, 367-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051151.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbiQ8BZL1.
PRIDEiQ8BZL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050890; ENSMUSP00000051151; ENSMUSG00000034000. [Q8BZL1-2]
ENSMUST00000190212; ENSMUSP00000140127; ENSMUSG00000034000. [Q8BZL1-1]
GeneIDi241159.
KEGGimmu:241159.
UCSCiuc007ceu.1. mouse. [Q8BZL1-2]

Organism-specific databases

CTDi129807.
MGIiMGI:2661364. Neu4.

Phylogenomic databases

eggNOGiNOG84455.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ8BZL1.
KOiK12357.
OMAiHIDCKEC.
OrthoDBiEOG7MSMNP.
TreeFamiTF331063.

Enzyme and pathway databases

ReactomeiREACT_310546. Sialic acid metabolism.
REACT_339900. Glycosphingolipid metabolism.

Miscellaneous databases

NextBioi384911.
PROiQ8BZL1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BZL1.
CleanExiMM_NEU4.
ExpressionAtlasiQ8BZL1. baseline.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR026946. Sialidase-4.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF8. PTHR10628:SF8. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression of Neu4, a novel murine sialidase."
    Comelli E.M., Amado M., Lustig S.R., Paulson J.C.
    Gene 321:155-161(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Diencephalon.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiNEUR4_MOUSE
AccessioniPrimary (citable) accession number: Q8BZL1
Secondary accession number(s): Q6NS66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 16, 2009
Last modified: June 24, 2015
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.