Reviewed, UniProtKB/Swiss-Prot Q8BZL1 (NEUR4_MOUSE)

Names and origin

Protein names

Recommended name:
    Sialidase-4
    EC=3.2.1.18
Alternative name(s):
    Neuraminidase 4
    N-acetyl-alpha-neuraminidase 4

Gene names

Name:

Neu4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed predominantly in brain. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 3 BNR repeats.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lysosome Inferred from sequence or structural similarity. Source: UniProtKB organelle inner membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	exo-alpha-sialidase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BZL1-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BZL1-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → METAGAPFCFHVDSLVPCSYWKVM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Sialidase-4		PRO_0000208907
Regions
	Repeat	127 – 138	12	BNR 1
	Repeat	200 – 211	12	BNR 2
	Repeat	247 – 258	12	BNR 3
	Motif	22 – 25	4	FRIP motif
Sites
	Active site	48	1	Proton acceptor By similarity
	Active site	413	1	Nucleophile By similarity
	Active site	434	1	Potential
	Binding site	23	1	Substrate By similarity
	Binding site	383	1	Substrate By similarity
Natural variations
	Alternative sequence	1	1	M → METAGAPFCFHVDSLVPCSY WKVM in isoform 2.		VSP_037492

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: E1833F5DBFBFD08F	FASTA	478	52,426
	10 20 30 40 50 60 MGPTRVPRRT VLFQRERTGL TYRVPALLCV PPRPTLLAFA EQRLSPDDSH AHRLVLRRGT 70 80 90 100 110 120 LTRGSVRWGT LSVLETAVLE EHRSMNPCPV LDEHSGTIFL FFIAVLGHTP EAVQIATGKN 130 140 150 160 170 180 AARLCCVTSC DAGLTWGSVR DLTEEAIGAA LQDWATFAVG PGHGVQLRSG RLLVPAYTYH 190 200 210 220 230 240 VDRRECFGKI CWTSPHSLAF YSDDHGISWH CGGLVPNLRS GECQLAAVDG DFLYCNARSP 250 260 270 280 290 300 LGNRVQALSA DEGTSFLPGE LVPTLAETAR GCQGSIVGFL APPSIEPQDD RWTGSPRNTP 310 320 330 340 350 360 HSPCFNLRVQ ESSGEGARGL LERWMPRLPL CYPQSRSPEN HGLEPGSDGD KTSWTPECPM 370 380 390 400 410 420 SSDSMLQSPT WLLYSHPAGR RARLHMGIYL SRSPLDPHSW TEPWVIYEGP SGYSDLAFLG 430 440 450 460 470 PMPGASLVFA CLFESGTRTS YEDISFCLFS LADVLENVPT GLEMLSLRDK AQGHCWPS
	Isoform 2. Checksum: 9CF4B78F19DF3BC2	FASTA	501	54,996
	10 20 30 40 50 60 METAGAPFCF HVDSLVPCSY WKVMGPTRVP RRTVLFQRER TGLTYRVPAL LCVPPRPTLL 70 80 90 100 110 120 AFAEQRLSPD DSHAHRLVLR RGTLTRGSVR WGTLSVLETA VLEEHRSMNP CPVLDEHSGT 130 140 150 160 170 180 IFLFFIAVLG HTPEAVQIAT GKNAARLCCV TSCDAGLTWG SVRDLTEEAI GAALQDWATF 190 200 210 220 230 240 AVGPGHGVQL RSGRLLVPAY TYHVDRRECF GKICWTSPHS LAFYSDDHGI SWHCGGLVPN 250 260 270 280 290 300 LRSGECQLAA VDGDFLYCNA RSPLGNRVQA LSADEGTSFL PGELVPTLAE TARGCQGSIV 310 320 330 340 350 360 GFLAPPSIEP QDDRWTGSPR NTPHSPCFNL RVQESSGEGA RGLLERWMPR LPLCYPQSRS 370 380 390 400 410 420 PENHGLEPGS DGDKTSWTPE CPMSSDSMLQ SPTWLLYSHP AGRRARLHMG IYLSRSPLDP 430 440 450 460 470 480 HSWTEPWVIY EGPSGYSDLA FLGPMPGASL VFACLFESGT RTSYEDISFC LFSLADVLEN 490 500 VPTGLEMLSL RDKAQGHCWP S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and expression of Neu4, a novel murine sialidase." Comelli E.M., Amado M., Lustig S.R., Paulson J.C. Gene 321:155-161(2003) [PubMed: 14637003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Diencephalon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY258421 mRNA. Translation: AAP81169.1. AK034236 mRNA. Translation: BAC28641.1.
IPI	IPI00227589. IPI00416833.
UniGene	Mm.214565
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
Genome annotation databases
Ensembl	ENSMUSG00000034000. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:2661364. Neu4.
Phylogenomic databases
HOGENOM	Q8BZL1.
HOVERGEN	Q8BZL1.
OMA	Q8BZL1. LTEEAIG.
Enzyme and pathway databases
BRENDA	3.2.1.18. 244.
Gene expression databases
ArrayExpress	Q8BZL1.
Bgee	Q8BZL1.
CleanEx	MM_NEU4.
Family and domain databases
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

NEUR4_MOUSE

Accession

Primary (citable) accession number: Q8BZL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 16, 2009
Last modified:	June 16, 2009
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)