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Q8BZL1 (NEUR4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-4

EC=3.2.1.18
Alternative name(s):
N-acetyl-alpha-neuraminidase 4
Neuraminidase 4
Gene names
Name:Neu4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids By similarity. Ref.1

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed predominantly in brain. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 3 BNR repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BZL1-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BZL1-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → METAGAPFCFHVDSLVPCSYWKVM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Sialidase-4
PRO_0000208907

Regions

Repeat127 – 13812BNR 1
Repeat200 – 21112BNR 2
Repeat247 – 25812BNR 3
Motif22 – 254FRIP motif

Sites

Active site471Proton acceptor By similarity
Active site481Proton acceptor By similarity
Active site4131Nucleophile By similarity
Active site4341 Potential
Binding site231Substrate By similarity
Binding site431Substrate By similarity
Binding site1771Substrate By similarity
Binding site1791Substrate By similarity
Binding site2221Substrate By similarity
Binding site2381Substrate By similarity
Binding site3831Substrate By similarity

Natural variations

Alternative sequence11M → METAGAPFCFHVDSLVPCSY WKVM in isoform 2.
VSP_037492

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: E1833F5DBFBFD08F

FASTA47852,426
        10         20         30         40         50         60 
MGPTRVPRRT VLFQRERTGL TYRVPALLCV PPRPTLLAFA EQRLSPDDSH AHRLVLRRGT 

        70         80         90        100        110        120 
LTRGSVRWGT LSVLETAVLE EHRSMNPCPV LDEHSGTIFL FFIAVLGHTP EAVQIATGKN 

       130        140        150        160        170        180 
AARLCCVTSC DAGLTWGSVR DLTEEAIGAA LQDWATFAVG PGHGVQLRSG RLLVPAYTYH 

       190        200        210        220        230        240 
VDRRECFGKI CWTSPHSLAF YSDDHGISWH CGGLVPNLRS GECQLAAVDG DFLYCNARSP 

       250        260        270        280        290        300 
LGNRVQALSA DEGTSFLPGE LVPTLAETAR GCQGSIVGFL APPSIEPQDD RWTGSPRNTP 

       310        320        330        340        350        360 
HSPCFNLRVQ ESSGEGARGL LERWMPRLPL CYPQSRSPEN HGLEPGSDGD KTSWTPECPM 

       370        380        390        400        410        420 
SSDSMLQSPT WLLYSHPAGR RARLHMGIYL SRSPLDPHSW TEPWVIYEGP SGYSDLAFLG 

       430        440        450        460        470 
PMPGASLVFA CLFESGTRTS YEDISFCLFS LADVLENVPT GLEMLSLRDK AQGHCWPS 

« Hide

Isoform 2 [UniParc].

Checksum: 9CF4B78F19DF3BC2
Show »

FASTA50154,996

References

« Hide 'large scale' references
[1]"Identification and expression of Neu4, a novel murine sialidase."
Comelli E.M., Amado M., Lustig S.R., Paulson J.C.
Gene 321:155-161(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Diencephalon.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY258421 mRNA. Translation: AAP81169.1.
AK034236 mRNA. Translation: BAC28641.1.
BC070438 mRNA. Translation: AAH70438.1.
CCDSCCDS15199.1. [Q8BZL1-2]
RefSeqNP_776133.1. NM_173772.3. [Q8BZL1-2]
XP_006529656.1. XM_006529593.1. [Q8BZL1-1]
UniGeneMm.214565.

3D structure databases

ProteinModelPortalQ8BZL1.
SMRQ8BZL1. Positions 22-284, 367-451.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbQ8BZL1.
PRIDEQ8BZL1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050890; ENSMUSP00000051151; ENSMUSG00000034000. [Q8BZL1-2]
GeneID241159.
KEGGmmu:241159.
UCSCuc007ceu.1. mouse. [Q8BZL1-2]

Organism-specific databases

CTD129807.
MGIMGI:2661364. Neu4.

Phylogenomic databases

eggNOGNOG84455.
GeneTreeENSGT00390000011171.
HOGENOMHOG000233778.
HOVERGENHBG052608.
InParanoidQ6NS66.
KOK12357.
OMAMNPCPVL.
OrthoDBEOG7MSMNP.
TreeFamTF331063.

Gene expression databases

BgeeQ8BZL1.
CleanExMM_NEU4.
GenevestigatorQ8BZL1.

Family and domain databases

Gene3D2.120.10.10. 2 hits.
InterProIPR026946. Sialidase-4.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF8. PTHR10628:SF8. 1 hit.
SUPFAMSSF50939. SSF50939. 2 hits.
ProtoNetSearch...

Other

NextBio384911.
PROQ8BZL1.
SOURCESearch...

Entry information

Entry nameNEUR4_MOUSE
AccessionPrimary (citable) accession number: Q8BZL1
Secondary accession number(s): Q6NS66
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries