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Q8BZL1

- NEUR4_MOUSE

UniProt

Q8BZL1 - NEUR4_MOUSE

Protein

Sialidase-4

Gene

Neu4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids By similarity.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei23 – 231SubstrateBy similarity
    Binding sitei43 – 431SubstrateBy similarity
    Active sitei47 – 471Proton acceptorBy similarity
    Active sitei48 – 481Proton acceptorBy similarity
    Binding sitei177 – 1771SubstrateBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei222 – 2221SubstrateBy similarity
    Binding sitei238 – 2381SubstrateBy similarity
    Binding sitei383 – 3831SubstrateBy similarity
    Active sitei413 – 4131NucleophileBy similarity
    Active sitei434 – 4341Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
    4. exo-alpha-sialidase activity Source: MGI

    GO - Biological processi

    1. ganglioside catabolic process Source: UniProtKB
    2. glycoprotein catabolic process Source: UniProtKB
    3. oligosaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-4 (EC:3.2.1.18)
    Alternative name(s):
    N-acetyl-alpha-neuraminidase 4
    Neuraminidase 4
    Gene namesi
    Name:Neu4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2661364. Neu4.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. lysosome Source: UniProtKB
    2. mitochondrion Source: MGI
    3. organelle inner membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Sialidase-4PRO_0000208907Add
    BLAST

    Proteomic databases

    PaxDbiQ8BZL1.
    PRIDEiQ8BZL1.

    Expressioni

    Tissue specificityi

    Expressed predominantly in brain.1 Publication

    Gene expression databases

    BgeeiQ8BZL1.
    CleanExiMM_NEU4.
    GenevestigatoriQ8BZL1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BZL1.
    SMRiQ8BZL1. Positions 22-284, 367-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati127 – 13812BNR 1Add
    BLAST
    Repeati200 – 21112BNR 2Add
    BLAST
    Repeati247 – 25812BNR 3Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi22 – 254FRIP motif

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 3 BNR repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG84455.
    GeneTreeiENSGT00390000011171.
    HOGENOMiHOG000233778.
    HOVERGENiHBG052608.
    InParanoidiQ6NS66.
    KOiK12357.
    OMAiMNPCPVL.
    OrthoDBiEOG7MSMNP.
    TreeFamiTF331063.

    Family and domain databases

    Gene3Di2.120.10.10. 2 hits.
    InterProiIPR026946. Sialidase-4.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF8. PTHR10628:SF8. 1 hit.
    SUPFAMiSSF50939. SSF50939. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BZL1-2) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGPTRVPRRT VLFQRERTGL TYRVPALLCV PPRPTLLAFA EQRLSPDDSH    50
    AHRLVLRRGT LTRGSVRWGT LSVLETAVLE EHRSMNPCPV LDEHSGTIFL 100
    FFIAVLGHTP EAVQIATGKN AARLCCVTSC DAGLTWGSVR DLTEEAIGAA 150
    LQDWATFAVG PGHGVQLRSG RLLVPAYTYH VDRRECFGKI CWTSPHSLAF 200
    YSDDHGISWH CGGLVPNLRS GECQLAAVDG DFLYCNARSP LGNRVQALSA 250
    DEGTSFLPGE LVPTLAETAR GCQGSIVGFL APPSIEPQDD RWTGSPRNTP 300
    HSPCFNLRVQ ESSGEGARGL LERWMPRLPL CYPQSRSPEN HGLEPGSDGD 350
    KTSWTPECPM SSDSMLQSPT WLLYSHPAGR RARLHMGIYL SRSPLDPHSW 400
    TEPWVIYEGP SGYSDLAFLG PMPGASLVFA CLFESGTRTS YEDISFCLFS 450
    LADVLENVPT GLEMLSLRDK AQGHCWPS 478
    Length:478
    Mass (Da):52,426
    Last modified:June 16, 2009 - v3
    Checksum:iE1833F5DBFBFD08F
    GO
    Isoform 2 (identifier: Q8BZL1-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → METAGAPFCFHVDSLVPCSYWKVM

    Note: No experimental confirmation available.

    Show »
    Length:501
    Mass (Da):54,996
    Checksum:i9CF4B78F19DF3BC2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → METAGAPFCFHVDSLVPCSY WKVM in isoform 2. 1 PublicationVSP_037492

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY258421 mRNA. Translation: AAP81169.1.
    AK034236 mRNA. Translation: BAC28641.1.
    BC070438 mRNA. Translation: AAH70438.1.
    CCDSiCCDS15199.1. [Q8BZL1-2]
    RefSeqiNP_776133.1. NM_173772.3. [Q8BZL1-2]
    XP_006529656.1. XM_006529593.1. [Q8BZL1-1]
    UniGeneiMm.214565.

    Genome annotation databases

    EnsembliENSMUST00000050890; ENSMUSP00000051151; ENSMUSG00000034000. [Q8BZL1-2]
    GeneIDi241159.
    KEGGimmu:241159.
    UCSCiuc007ceu.1. mouse. [Q8BZL1-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY258421 mRNA. Translation: AAP81169.1 .
    AK034236 mRNA. Translation: BAC28641.1 .
    BC070438 mRNA. Translation: AAH70438.1 .
    CCDSi CCDS15199.1. [Q8BZL1-2 ]
    RefSeqi NP_776133.1. NM_173772.3. [Q8BZL1-2 ]
    XP_006529656.1. XM_006529593.1. [Q8BZL1-1 ]
    UniGenei Mm.214565.

    3D structure databases

    ProteinModelPortali Q8BZL1.
    SMRi Q8BZL1. Positions 22-284, 367-451.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    Proteomic databases

    PaxDbi Q8BZL1.
    PRIDEi Q8BZL1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050890 ; ENSMUSP00000051151 ; ENSMUSG00000034000 . [Q8BZL1-2 ]
    GeneIDi 241159.
    KEGGi mmu:241159.
    UCSCi uc007ceu.1. mouse. [Q8BZL1-2 ]

    Organism-specific databases

    CTDi 129807.
    MGIi MGI:2661364. Neu4.

    Phylogenomic databases

    eggNOGi NOG84455.
    GeneTreei ENSGT00390000011171.
    HOGENOMi HOG000233778.
    HOVERGENi HBG052608.
    InParanoidi Q6NS66.
    KOi K12357.
    OMAi MNPCPVL.
    OrthoDBi EOG7MSMNP.
    TreeFami TF331063.

    Enzyme and pathway databases

    Reactomei REACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 384911.
    PROi Q8BZL1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BZL1.
    CleanExi MM_NEU4.
    Genevestigatori Q8BZL1.

    Family and domain databases

    Gene3Di 2.120.10.10. 2 hits.
    InterProi IPR026946. Sialidase-4.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF8. PTHR10628:SF8. 1 hit.
    SUPFAMi SSF50939. SSF50939. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and expression of Neu4, a novel murine sialidase."
      Comelli E.M., Amado M., Lustig S.R., Paulson J.C.
      Gene 321:155-161(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Diencephalon.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiNEUR4_MOUSE
    AccessioniPrimary (citable) accession number: Q8BZL1
    Secondary accession number(s): Q6NS66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 91 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3