Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin filament-associated protein 1-like 1

Gene

Afap1l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in podosome and invadosome formation.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1-like 1
Short name:
AFAP1-like protein 1
Gene namesi
Name:Afap1l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2147199. Afap1l1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Actin filament-associated protein 1-like 1PRO_0000295240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei93 – 931PhosphoserineBy similarity
Modified residuei97 – 971PhosphoserineBy similarity
Modified residuei103 – 1031PhosphoserineBy similarity
Modified residuei153 – 1531PhosphoserineCombined sources
Modified residuei329 – 3291PhosphoserineCombined sources
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei557 – 5571PhosphotyrosineCombined sources
Modified residuei747 – 7471PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BZI0.
PaxDbiQ8BZI0.
PRIDEiQ8BZI0.

PTM databases

iPTMnetiQ8BZI0.
PhosphoSiteiQ8BZI0.

Expressioni

Gene expression databases

BgeeiQ8BZI0.
CleanExiMM_AFAP1L1.
ExpressionAtlasiQ8BZI0. baseline and differential.
GenevisibleiQ8BZI0. MM.

Interactioni

Subunit structurei

Interacts with CTTN.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113286.

Structurei

3D structure databases

ProteinModelPortaliQ8BZI0.
SMRiQ8BZI0. Positions 424-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini220 – 31697PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini418 – 51295PH 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili611 – 70191Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi194 – 1996Poly-Glu

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ8BZI0.
OMAiGRRETCD.
OrthoDBiEOG7KQ217.
PhylomeDBiQ8BZI0.
TreeFamiTF332622.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030112. AFAP1L1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF1. PTHR14338:SF1. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZI0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRSRVLEQL IPELTGLLSL LDHEYLSDST LEKKMAVASL LQSLQPLPAK
60 70 80 90 100
EVSFLYVNTA DLHSGPSFVE SLFEEFDCDL GDLRDMSDDG EPSKGASPEP
110 120 130 140 150
TKSPSLRSAA ADVPPPLPNK PPPEDYYEEA LPLGPGKSPE YISSHNGCSP
160 170 180 190 200
AQSIVDGYYE DADNSYPTTR MNGELKNSYN DSDAMSSSYE SYDEEEEEEK
210 220 230 240 250
GRQPKHQWPS EEASMHLVRD CRICAFLLRK KRFGQWAKQL TVIKEEQLLC
260 270 280 290 300
YKSSKDRQPH LRLALDVCTV IYVPKDSRHK RHELRFSQGA TEVLVLALQS
310 320 330 340 350
REQAEEWLKV IREVSRPIVG AEGLEVPRSP VILCKADQDK RLSQEKQNSD
360 370 380 390 400
SDSLGMNDSG STLGRREACE HGKGKKNSLA ELKGSMSRAA GRKITRIISF
410 420 430 440 450
SKKKALSEDL QTFSSEDEVP CCGYLNVLVN QGWKERWCRL RCNTLYFHKD
460 470 480 490 500
RTDLHTHVNS IALRGCEVAP GFGPRHPFAF RILRNRQEVA ILEASCSEDM
510 520 530 540 550
GRWLGLLLVE MGSKVTPEAL HYDYVDVETL TSIVSAGRNS FLYAQSCQDQ
560 570 580 590 600
WPEPRIYDEV PYEKVQDEEP QRPTGAQVKR HASSCSEKSH RADPQVKVKR
610 620 630 640 650
HASSANQYKY GKNRAEEDAR RYLVEKERLE KEKETIRTEL TALRQEKKEL
660 670 680 690 700
KEAIRNNPGA KSKALEEAVA TLEAQCRAKE EQRIDLELKL VAVKERLQQS
710 720 730 740 750
LAGGPALGLS VSNKNKSQDT TNKPQSNAPE QSLPVNCVSE LRKRSPSIVT
760
SNQGRVLQKA KEWEMKKT
Length:768
Mass (Da):86,638
Last modified:March 1, 2003 - v1
Checksum:i5913993426EBCFB2
GO
Isoform 2 (identifier: Q8BZI0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     373-458: KGKKNSLAEL...KDRTDLHTHV → RSYHELPLVH...RTAWLSLRAP
     459-768: Missing.

Note: No experimental confirmation available.
Show »
Length:464
Mass (Da):51,943
Checksum:i071F580415B167E6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei373 – 45886KGKKN…LHTHV → RSYHELPLVHAGECQRWEGL CYRSTVTEQITGTRVRPLGL SYLVITGRLQGLLGRPEVGR SGTVASFPIPVSPLLWCQAK GRRTAWLSLRAP in isoform 2. 1 PublicationVSP_026860Add
BLAST
Alternative sequencei459 – 768310Missing in isoform 2. 1 PublicationVSP_026861Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035227 mRNA. Translation: BAC28988.1.
AK053714 mRNA. Translation: BAC35486.1.
CCDSiCCDS50303.1. [Q8BZI0-1]
RefSeqiNP_849259.2. NM_178928.4. [Q8BZI0-1]
UniGeneiMm.26632.

Genome annotation databases

EnsembliENSMUST00000120472; ENSMUSP00000113286; ENSMUSG00000033032. [Q8BZI0-1]
GeneIDi106877.
KEGGimmu:106877.
UCSCiuc008fcq.1. mouse. [Q8BZI0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035227 mRNA. Translation: BAC28988.1.
AK053714 mRNA. Translation: BAC35486.1.
CCDSiCCDS50303.1. [Q8BZI0-1]
RefSeqiNP_849259.2. NM_178928.4. [Q8BZI0-1]
UniGeneiMm.26632.

3D structure databases

ProteinModelPortaliQ8BZI0.
SMRiQ8BZI0. Positions 424-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113286.

PTM databases

iPTMnetiQ8BZI0.
PhosphoSiteiQ8BZI0.

Proteomic databases

MaxQBiQ8BZI0.
PaxDbiQ8BZI0.
PRIDEiQ8BZI0.

Protocols and materials databases

DNASUi106877.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120472; ENSMUSP00000113286; ENSMUSG00000033032. [Q8BZI0-1]
GeneIDi106877.
KEGGimmu:106877.
UCSCiuc008fcq.1. mouse. [Q8BZI0-1]

Organism-specific databases

CTDi134265.
MGIiMGI:2147199. Afap1l1.

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ8BZI0.
OMAiGRRETCD.
OrthoDBiEOG7KQ217.
PhylomeDBiQ8BZI0.
TreeFamiTF332622.

Miscellaneous databases

NextBioi358446.
PROiQ8BZI0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BZI0.
CleanExiMM_AFAP1L1.
ExpressionAtlasiQ8BZI0. baseline and differential.
GenevisibleiQ8BZI0. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030112. AFAP1L1.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF1. PTHR14338:SF1. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Urinary bladder.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153; SER-329; TYR-557 AND SER-747, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney and Lung.

Entry informationi

Entry nameiAF1L1_MOUSE
AccessioniPrimary (citable) accession number: Q8BZI0
Secondary accession number(s): Q8BKB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.