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Q8BZH1

- TGM4_MOUSE

UniProt

Q8BZH1 - TGM4_MOUSE

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Protein
Protein-glutamine gamma-glutamyltransferase 4
Gene
Tgm4, Eapa1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Associated with the mammalian reproductive process. Plays an important role in the formation of the seminal coagulum through the cross-linking of specific proteins present in the seminal plasma. Transglutaminase is also required to stabilize the copulatory plug.1 Publication

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.1 Publication

Cofactori

Binds 1 calcium ion per subunit By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551 By similarityBy similarity
Active sitei314 – 3141 By similarityBy similarity
Active sitei337 – 3371 By similarityBy similarity
Metal bindingi377 – 3771Calcium By similarityBy similarity
Metal bindingi379 – 3791Calcium By similarityBy similarity
Metal bindingi429 – 4291Calcium By similarityBy similarity
Metal bindingi434 – 4341Calcium By similarityBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mating plug formation Source: MGI
  2. peptide cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase 4 (EC:2.3.2.13)
Alternative name(s):
Experimental autoimmune prostatitis antigen 1
Transglutaminase-4
Short name:
TGase-4
Gene namesi
Name:Tgm4
Synonyms:Eapa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:3027002. Tgm4.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Copulatory plug, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670Protein-glutamine gamma-glutamyltransferase 4
PRO_0000385448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi151 – 1511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi219 – 2191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi288 – 2881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi456 – 4561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi491 – 4911N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8BZH1.

Expressioni

Tissue specificityi

Expressed in the coagulating gland and in the dorsal part of the prostate. Not expressed in the brain, heart, kidney, liver, lung, muscle, pancreas, spleen, stomach, testis and thymus.2 Publications

Gene expression databases

BgeeiQ8BZH1.
GenevestigatoriQ8BZH1.

Interactioni

Subunit structurei

Homodimer By similarity.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026893.

Structurei

3D structure databases

ProteinModelPortaliQ8BZH1.
SMRiQ8BZH1. Positions 5-670.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG80379.
GeneTreeiENSGT00740000115156.
HOGENOMiHOG000231695.
InParanoidiQ8BZH1.
KOiK05621.
OrthoDBiEOG7WT40M.
PhylomeDBiQ8BZH1.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BZH1-1 [UniParc]FASTAAdd to Basket

« Hide

MDSRNVLIIY AVNVERKLNA AAHHTSEYQT KKLVLRRGQI FTLKVILNRP    50
LQPQDELKVT FTSGQRDPPY MVELDPVTSY RSKGWQVKIA KQSGVEVILN 100
VISAADAVVG RYKMRVNEYK AGVFYLLFNP WCSDDSVFMA SEEERAEYIL 150
NDTGYMYMGF AKQIKEKPWT FGQFEKHILS CCFNLLFQLE NNEMQNPVLV 200
SRAICTMMCA ANGGVLMGNW TGDYADGTAP YVWTSSVPIL QQHYVTRMPV 250
RYGQCWVFSG ILTTALRAVG IPARSVTNFE SAHDTEKNLT VDIYLDESGK 300
TIPHLTKDSV WNFHVWTDAW MKRQDLPHGY DGWQVLDSTP QEISDGGFRT 350
GPSPLTAIRQ GLIQMKYDTT FVFTEVNGDK FIWLVKQNQE REKNILIAVE 400
TASLGKKIST KMVGENRRED ITLQYRFPEG SPEERKVMAK ASGKPSDDKL 450
NSRTLNNSLQ ISVLQNSLEL GAPIYLTITL KRKTATPQNV NISCSLNLQT 500
YTGNKKTNLG VIQKTVQIHG QESRVFLTMD ASYYIYKLGM VDDEMVIGGF 550
IIAEIVDSGE RVATDTTLCF LYSAFSVEMP STGKVKQPLV ITSKFTNTLP 600
IPLTNIKFSV ESLGLANMKS WEQETVPPGK TITFQMECTP VKAGPQKFIV 650
KFISRQVKEV HAEKVVLISK 670
Length:670
Mass (Da):75,591
Last modified:September 22, 2009 - v2
Checksum:iAEAD1A23E3D97EC4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341D → G in BAC29013. 1 Publication
Sequence conflicti404 – 4041L → I in BAC29013. 1 Publication
Sequence conflicti404 – 4041L → I AA sequence 1 Publication
Sequence conflicti426 – 4261R → K in BAC29013. 1 Publication
Sequence conflicti426 – 4261R → K AA sequence 1 Publication
Sequence conflicti528 – 5281T → A in BAC29013. 1 Publication
Sequence conflicti537 – 5371K → E in BAC29013. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF486627 mRNA. Translation: AAM45940.1.
AK035279 mRNA. Translation: BAC29013.1.
BC141297 mRNA. Translation: AAI41298.1.
BC145622 mRNA. Translation: AAI45623.1.
CCDSiCCDS23653.1.
RefSeqiNP_808579.2. NM_177911.4.
UniGeneiMm.195309.

Genome annotation databases

EnsembliENSMUST00000026893; ENSMUSP00000026893; ENSMUSG00000025787.
GeneIDi331046.
KEGGimmu:331046.
UCSCiuc009sfo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF486627 mRNA. Translation: AAM45940.1 .
AK035279 mRNA. Translation: BAC29013.1 .
BC141297 mRNA. Translation: AAI41298.1 .
BC145622 mRNA. Translation: AAI45623.1 .
CCDSi CCDS23653.1.
RefSeqi NP_808579.2. NM_177911.4.
UniGenei Mm.195309.

3D structure databases

ProteinModelPortali Q8BZH1.
SMRi Q8BZH1. Positions 5-670.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000026893.

Proteomic databases

PRIDEi Q8BZH1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026893 ; ENSMUSP00000026893 ; ENSMUSG00000025787 .
GeneIDi 331046.
KEGGi mmu:331046.
UCSCi uc009sfo.1. mouse.

Organism-specific databases

CTDi 7047.
MGIi MGI:3027002. Tgm4.

Phylogenomic databases

eggNOGi NOG80379.
GeneTreei ENSGT00740000115156.
HOGENOMi HOG000231695.
InParanoidi Q8BZH1.
KOi K05621.
OrthoDBi EOG7WT40M.
PhylomeDBi Q8BZH1.
TreeFami TF324278.

Miscellaneous databases

ChiTaRSi TGM4. mouse.
NextBioi 399704.
PROi Q8BZH1.
SOURCEi Search...

Gene expression databases

Bgeei Q8BZH1.
Genevestigatori Q8BZH1.

Family and domain databases

Gene3Di 2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view ]
PANTHERi PTHR11590. PTHR11590. 1 hit.
Pfami PF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
SMARTi SM00460. TGc. 1 hit.
[Graphical view ]
SUPFAMi SSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Physiologic self antigens rapidly capacitate autoimmune disease-specific polyclonal CD4+ CD25+ regulatory T cells."
    Setiady Y.Y., Ohno K., Samy E.T., Bagavant H., Qiao H., Sharp C., She J.X., Tung K.S.K.
    Blood 107:1056-1062(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Urinary bladder.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins."
    Tseng H.-C., Lin H.-J., Sudhakar Gandhi P.S., Wang C.-Y., Chen Y.-H.
    J. Chromatogr. B 876:198-202(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-19; 37-58; 67-81; 146-162; 166-176; 288-300; 308-322; 324-359; 368-386; 394-406; 418-435; 484-500; 525-536 AND 595-642, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Coagulating gland secretion.

Entry informationi

Entry nameiTGM4_MOUSE
AccessioniPrimary (citable) accession number: Q8BZH1
Secondary accession number(s): B7ZP44, Q8K460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi