Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-glutamine gamma-glutamyltransferase 4

Gene

Tgm4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Associated with the mammalian reproductive process. Plays an important role in the formation of the seminal coagulum through the cross-linking of specific proteins present in the seminal plasma. Transglutaminase is also required to stabilize the copulatory plug.1 Publication

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551PROSITE-ProRule annotationBy similarity
Active sitei314 – 3141PROSITE-ProRule annotationBy similarity
Active sitei337 – 3371PROSITE-ProRule annotationBy similarity
Metal bindingi377 – 3771CalciumBy similarity
Metal bindingi379 – 3791CalciumBy similarity
Metal bindingi429 – 4291CalciumBy similarity
Metal bindingi434 – 4341CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

  • mating plug formation Source: MGI
  • peptide cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.3.2.13. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase 41 Publication (EC:2.3.2.13)
Alternative name(s):
Experimental autoimmune prostatitis antigen 11 Publication
Transglutaminase-41 Publication
Short name:
TGase-4By similarity
Gene namesi
Name:Tgm4Imported
Synonyms:Eapa1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3027002. Tgm4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Copulatory plug, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670Protein-glutamine gamma-glutamyltransferase 4PRO_0000385448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8BZH1.

Expressioni

Tissue specificityi

Expressed in the coagulating gland and in the dorsal part of the prostate. Not expressed in the brain, heart, kidney, liver, lung, muscle, pancreas, spleen, stomach, testis and thymus.2 Publications

Gene expression databases

BgeeiQ8BZH1.
GenevestigatoriQ8BZH1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026893.

Structurei

3D structure databases

ProteinModelPortaliQ8BZH1.
SMRiQ8BZH1. Positions 5-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transglutaminase superfamily. Transglutaminase family.Sequence Analysis

Phylogenomic databases

eggNOGiNOG80379.
HOGENOMiHOG000231695.
InParanoidiQ8BZH1.
KOiK05621.
OrthoDBiEOG7WT40M.
PhylomeDBiQ8BZH1.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BZH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRNVLIIY AVNVERKLNA AAHHTSEYQT KKLVLRRGQI FTLKVILNRP
60 70 80 90 100
LQPQDELKVT FTSGQRDPPY MVELDPVTSY RSKGWQVKIA KQSGVEVILN
110 120 130 140 150
VISAADAVVG RYKMRVNEYK AGVFYLLFNP WCSDDSVFMA SEEERAEYIL
160 170 180 190 200
NDTGYMYMGF AKQIKEKPWT FGQFEKHILS CCFNLLFQLE NNEMQNPVLV
210 220 230 240 250
SRAICTMMCA ANGGVLMGNW TGDYADGTAP YVWTSSVPIL QQHYVTRMPV
260 270 280 290 300
RYGQCWVFSG ILTTALRAVG IPARSVTNFE SAHDTEKNLT VDIYLDESGK
310 320 330 340 350
TIPHLTKDSV WNFHVWTDAW MKRQDLPHGY DGWQVLDSTP QEISDGGFRT
360 370 380 390 400
GPSPLTAIRQ GLIQMKYDTT FVFTEVNGDK FIWLVKQNQE REKNILIAVE
410 420 430 440 450
TASLGKKIST KMVGENRRED ITLQYRFPEG SPEERKVMAK ASGKPSDDKL
460 470 480 490 500
NSRTLNNSLQ ISVLQNSLEL GAPIYLTITL KRKTATPQNV NISCSLNLQT
510 520 530 540 550
YTGNKKTNLG VIQKTVQIHG QESRVFLTMD ASYYIYKLGM VDDEMVIGGF
560 570 580 590 600
IIAEIVDSGE RVATDTTLCF LYSAFSVEMP STGKVKQPLV ITSKFTNTLP
610 620 630 640 650
IPLTNIKFSV ESLGLANMKS WEQETVPPGK TITFQMECTP VKAGPQKFIV
660 670
KFISRQVKEV HAEKVVLISK
Length:670
Mass (Da):75,591
Last modified:September 22, 2009 - v2
Checksum:iAEAD1A23E3D97EC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341D → G in BAC29013 (PubMed:16141072).Curated
Sequence conflicti404 – 4041L → I in BAC29013 (PubMed:16141072).Curated
Sequence conflicti404 – 4041L → I AA sequence (PubMed:19027372).Curated
Sequence conflicti426 – 4261R → K in BAC29013 (PubMed:16141072).Curated
Sequence conflicti426 – 4261R → K AA sequence (PubMed:19027372).Curated
Sequence conflicti528 – 5281T → A in BAC29013 (PubMed:16141072).Curated
Sequence conflicti537 – 5371K → E in BAC29013 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486627 mRNA. Translation: AAM45940.1.
AK035279 mRNA. Translation: BAC29013.1.
BC141297 mRNA. Translation: AAI41298.1.
BC145622 mRNA. Translation: AAI45623.1.
CCDSiCCDS23653.1.
RefSeqiNP_808579.2. NM_177911.4.
UniGeneiMm.195309.

Genome annotation databases

GeneIDi331046.
KEGGimmu:331046.
UCSCiuc009sfo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486627 mRNA. Translation: AAM45940.1.
AK035279 mRNA. Translation: BAC29013.1.
BC141297 mRNA. Translation: AAI41298.1.
BC145622 mRNA. Translation: AAI45623.1.
CCDSiCCDS23653.1.
RefSeqiNP_808579.2. NM_177911.4.
UniGeneiMm.195309.

3D structure databases

ProteinModelPortaliQ8BZH1.
SMRiQ8BZH1. Positions 5-670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026893.

Proteomic databases

PRIDEiQ8BZH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi331046.
KEGGimmu:331046.
UCSCiuc009sfo.1. mouse.

Organism-specific databases

CTDi7047.
MGIiMGI:3027002. Tgm4.

Phylogenomic databases

eggNOGiNOG80379.
HOGENOMiHOG000231695.
InParanoidiQ8BZH1.
KOiK05621.
OrthoDBiEOG7WT40M.
PhylomeDBiQ8BZH1.
TreeFamiTF324278.

Enzyme and pathway databases

BRENDAi2.3.2.13. 3474.

Miscellaneous databases

ChiTaRSiTgm4. mouse.
NextBioi399704.
PROiQ8BZH1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BZH1.
GenevestigatoriQ8BZH1.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Physiologic self antigens rapidly capacitate autoimmune disease-specific polyclonal CD4+ CD25+ regulatory T cells."
    Setiady Y.Y., Ohno K., Samy E.T., Bagavant H., Qiao H., Sharp C., She J.X., Tung K.S.K.
    Blood 107:1056-1062(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6Imported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: Urinary bladderImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins."
    Tseng H.-C., Lin H.-J., Sudhakar Gandhi P.S., Wang C.-Y., Chen Y.-H.
    J. Chromatogr. B 876:198-202(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-19; 37-58; 67-81; 146-162; 166-176; 288-300; 308-322; 324-359; 368-386; 394-406; 418-435; 484-500; 525-536 AND 595-642, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Coagulating gland secretion1 Publication.

Entry informationi

Entry nameiTGM4_MOUSE
AccessioniPrimary (citable) accession number: Q8BZH1
Secondary accession number(s): B7ZP44, Q8K460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: May 27, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.