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Q8BZH1

- TGM4_MOUSE

UniProt

Q8BZH1 - TGM4_MOUSE

Protein

Protein-glutamine gamma-glutamyltransferase 4

Gene

Tgm4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Associated with the mammalian reproductive process. Plays an important role in the formation of the seminal coagulum through the cross-linking of specific proteins present in the seminal plasma. Transglutaminase is also required to stabilize the copulatory plug.1 Publication

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.1 PublicationPROSITE-ProRule annotation

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei255 – 2551By similarityPROSITE-ProRule annotation
    Active sitei314 – 3141By similarityPROSITE-ProRule annotation
    Active sitei337 – 3371By similarityPROSITE-ProRule annotation
    Metal bindingi377 – 3771CalciumBy similarity
    Metal bindingi379 – 3791CalciumBy similarity
    Metal bindingi429 – 4291CalciumBy similarity
    Metal bindingi434 – 4341CalciumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mating plug formation Source: MGI
    2. peptide cross-linking Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase 41 Publication (EC:2.3.2.13)
    Alternative name(s):
    Experimental autoimmune prostatitis antigen 11 Publication
    Transglutaminase-41 Publication
    Short name:
    TGase-4By similarity
    Gene namesi
    Name:Tgm4Imported
    Synonyms:Eapa1Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:3027002. Tgm4.

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Copulatory plug, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 670670Protein-glutamine gamma-glutamyltransferase 4PRO_0000385448Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8BZH1.

    Expressioni

    Tissue specificityi

    Expressed in the coagulating gland and in the dorsal part of the prostate. Not expressed in the brain, heart, kidney, liver, lung, muscle, pancreas, spleen, stomach, testis and thymus.2 Publications

    Gene expression databases

    BgeeiQ8BZH1.
    GenevestigatoriQ8BZH1.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026893.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BZH1.
    SMRiQ8BZH1. Positions 5-670.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the transglutaminase superfamily. Transglutaminase family.Sequence Analysis

    Phylogenomic databases

    eggNOGiNOG80379.
    GeneTreeiENSGT00740000115156.
    HOGENOMiHOG000231695.
    InParanoidiQ8BZH1.
    KOiK05621.
    OrthoDBiEOG7WT40M.
    PhylomeDBiQ8BZH1.
    TreeFamiTF324278.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view]
    PANTHERiPTHR11590. PTHR11590. 1 hit.
    PfamiPF00927. Transglut_C. 1 hit.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BZH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSRNVLIIY AVNVERKLNA AAHHTSEYQT KKLVLRRGQI FTLKVILNRP    50
    LQPQDELKVT FTSGQRDPPY MVELDPVTSY RSKGWQVKIA KQSGVEVILN 100
    VISAADAVVG RYKMRVNEYK AGVFYLLFNP WCSDDSVFMA SEEERAEYIL 150
    NDTGYMYMGF AKQIKEKPWT FGQFEKHILS CCFNLLFQLE NNEMQNPVLV 200
    SRAICTMMCA ANGGVLMGNW TGDYADGTAP YVWTSSVPIL QQHYVTRMPV 250
    RYGQCWVFSG ILTTALRAVG IPARSVTNFE SAHDTEKNLT VDIYLDESGK 300
    TIPHLTKDSV WNFHVWTDAW MKRQDLPHGY DGWQVLDSTP QEISDGGFRT 350
    GPSPLTAIRQ GLIQMKYDTT FVFTEVNGDK FIWLVKQNQE REKNILIAVE 400
    TASLGKKIST KMVGENRRED ITLQYRFPEG SPEERKVMAK ASGKPSDDKL 450
    NSRTLNNSLQ ISVLQNSLEL GAPIYLTITL KRKTATPQNV NISCSLNLQT 500
    YTGNKKTNLG VIQKTVQIHG QESRVFLTMD ASYYIYKLGM VDDEMVIGGF 550
    IIAEIVDSGE RVATDTTLCF LYSAFSVEMP STGKVKQPLV ITSKFTNTLP 600
    IPLTNIKFSV ESLGLANMKS WEQETVPPGK TITFQMECTP VKAGPQKFIV 650
    KFISRQVKEV HAEKVVLISK 670
    Length:670
    Mass (Da):75,591
    Last modified:September 22, 2009 - v2
    Checksum:iAEAD1A23E3D97EC4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti134 – 1341D → G in BAC29013. (PubMed:16141072)Curated
    Sequence conflicti404 – 4041L → I in BAC29013. (PubMed:16141072)Curated
    Sequence conflicti404 – 4041L → I AA sequence (PubMed:19027372)Curated
    Sequence conflicti426 – 4261R → K in BAC29013. (PubMed:16141072)Curated
    Sequence conflicti426 – 4261R → K AA sequence (PubMed:19027372)Curated
    Sequence conflicti528 – 5281T → A in BAC29013. (PubMed:16141072)Curated
    Sequence conflicti537 – 5371K → E in BAC29013. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486627 mRNA. Translation: AAM45940.1.
    AK035279 mRNA. Translation: BAC29013.1.
    BC141297 mRNA. Translation: AAI41298.1.
    BC145622 mRNA. Translation: AAI45623.1.
    CCDSiCCDS23653.1.
    RefSeqiNP_808579.2. NM_177911.4.
    UniGeneiMm.195309.

    Genome annotation databases

    EnsembliENSMUST00000026893; ENSMUSP00000026893; ENSMUSG00000025787.
    GeneIDi331046.
    KEGGimmu:331046.
    UCSCiuc009sfo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486627 mRNA. Translation: AAM45940.1 .
    AK035279 mRNA. Translation: BAC29013.1 .
    BC141297 mRNA. Translation: AAI41298.1 .
    BC145622 mRNA. Translation: AAI45623.1 .
    CCDSi CCDS23653.1.
    RefSeqi NP_808579.2. NM_177911.4.
    UniGenei Mm.195309.

    3D structure databases

    ProteinModelPortali Q8BZH1.
    SMRi Q8BZH1. Positions 5-670.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026893.

    Proteomic databases

    PRIDEi Q8BZH1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026893 ; ENSMUSP00000026893 ; ENSMUSG00000025787 .
    GeneIDi 331046.
    KEGGi mmu:331046.
    UCSCi uc009sfo.1. mouse.

    Organism-specific databases

    CTDi 7047.
    MGIi MGI:3027002. Tgm4.

    Phylogenomic databases

    eggNOGi NOG80379.
    GeneTreei ENSGT00740000115156.
    HOGENOMi HOG000231695.
    InParanoidi Q8BZH1.
    KOi K05621.
    OrthoDBi EOG7WT40M.
    PhylomeDBi Q8BZH1.
    TreeFami TF324278.

    Miscellaneous databases

    ChiTaRSi TGM4. mouse.
    NextBioi 399704.
    PROi Q8BZH1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BZH1.
    Genevestigatori Q8BZH1.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view ]
    PANTHERi PTHR11590. PTHR11590. 1 hit.
    Pfami PF00927. Transglut_C. 1 hit.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Physiologic self antigens rapidly capacitate autoimmune disease-specific polyclonal CD4+ CD25+ regulatory T cells."
      Setiady Y.Y., Ohno K., Samy E.T., Bagavant H., Qiao H., Sharp C., She J.X., Tung K.S.K.
      Blood 107:1056-1062(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6Imported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: Urinary bladderImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins."
      Tseng H.-C., Lin H.-J., Sudhakar Gandhi P.S., Wang C.-Y., Chen Y.-H.
      J. Chromatogr. B 876:198-202(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 5-19; 37-58; 67-81; 146-162; 166-176; 288-300; 308-322; 324-359; 368-386; 394-406; 418-435; 484-500; 525-536 AND 595-642, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Coagulating gland secretion1 Publication.

    Entry informationi

    Entry nameiTGM4_MOUSE
    AccessioniPrimary (citable) accession number: Q8BZH1
    Secondary accession number(s): B7ZP44, Q8K460
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3