Reviewed, UniProtKB/Swiss-Prot Q8BZ98 (DYN3_MOUSE)

Names and origin

Protein names

Recommended name:
    Dynamin-3
    EC=3.6.5.5

Gene names

Name:	Dnm3
Synonyms:	Kiaa0820

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis By similarity.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: Microtubule-associated By similarity.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: MGI
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BZ98-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BZ98-2)

The sequence of this isoform differs from the canonical sequence as follows:
     628-631: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	Dynamin-3		PRO_0000319951
Regions
	Domain	515 – 621	107	PH
	Domain	653 – 744	92	GED
	Nucleotide binding	38 – 45	8	GTP By similarity
	Nucleotide binding	136 – 140	5	GTP By similarity
	Nucleotide binding	205 – 208	4	GTP By similarity
Amino acid modifications
	Modified residue	594	1	N6-acetyllysine By similarity
	Modified residue	763	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	628 – 631	4	Missing in isoform 2.		VSP_031548
Experimental info
	Sequence conflict	139	1	G → A in BAE28068. Ref.1
	Sequence conflict	532 – 549	18	Missing in BAC65661. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8BDBB091CB10D264	FASTA	863	97,190
	10 20 30 40 50 60 MGNREMEELI PLVNRLQDAF SALGQSCLLE LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVTSKAEY AEFLHCKGKK FTDFDEVRHE IEAETDRVTG MNKGISSIPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIRDMI MQFITRENCL ILAVTPANTD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YVGVVNRSQK 250 260 270 280 290 300 DIDGKKDIKA AMLAERKFFL SHPAYRHIAD RMGTPHLQKV LNQQLTNHIR DTLPNFRNKL 310 320 330 340 350 360 QGQLLSIEHE VEAFKNFKPE DPTRKTKALL QMVQQFAVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 KINRIFHERF PFEIVKMEFN EKELRREISY AIKNIHGIRT GLFTPDMAFE AIVKKQIVKL 430 440 450 460 470 480 KGPSLKSVDL VMQELINTVK KCTKRLANFP RLCEETERIV ANHIREREGK TKDQVLLLID 490 500 510 520 530 540 IQVSYINTNH EDFIGFANAQ QRSSQVHKKS TIGNQVIRKG WLTVSNIGIM KGGSKGYWFV 550 560 570 580 590 600 LTAESLSWYK DDEEKEKKYM LPLDNLKVRD VEKGFMSSKH VFALFNTEQR NVYKDYRFLE 610 620 630 640 650 660 LACDSQEDVD SWKASLLRAG VYPDKSVGSN KTENDENGQA ENFSMDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYMSIINKC IRDLIPKTIM HLMINNVKDF INSELLAQLY SSEDQNTLME ESAEQAQRRD 730 740 750 760 770 780 EMLRMYQALK EALAIIGDIN TATVSTPAPP PVDDSWLQHS RRSPPPSPTT QRRLTISAPL 790 800 810 820 830 840 PRPTSGRGPA PAIPSPGPHS GAPPVPFRPG PLPPFPNSSD SFGAPPQVPS RPTRAPPSVP 850 860 SRRPPPSPTR PTIIRPLESS LLD
	Isoform 2. Checksum: 591E1B65A81A26D0	FASTA	859	96,804
	10 20 30 40 50 60 MGNREMEELI PLVNRLQDAF SALGQSCLLE LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVTSKAEY AEFLHCKGKK FTDFDEVRHE IEAETDRVTG MNKGISSIPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIRDMI MQFITRENCL ILAVTPANTD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YVGVVNRSQK 250 260 270 280 290 300 DIDGKKDIKA AMLAERKFFL SHPAYRHIAD RMGTPHLQKV LNQQLTNHIR DTLPNFRNKL 310 320 330 340 350 360 QGQLLSIEHE VEAFKNFKPE DPTRKTKALL QMVQQFAVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 KINRIFHERF PFEIVKMEFN EKELRREISY AIKNIHGIRT GLFTPDMAFE AIVKKQIVKL 430 440 450 460 470 480 KGPSLKSVDL VMQELINTVK KCTKRLANFP RLCEETERIV ANHIREREGK TKDQVLLLID 490 500 510 520 530 540 IQVSYINTNH EDFIGFANAQ QRSSQVHKKS TIGNQVIRKG WLTVSNIGIM KGGSKGYWFV 550 560 570 580 590 600 LTAESLSWYK DDEEKEKKYM LPLDNLKVRD VEKGFMSSKH VFALFNTEQR NVYKDYRFLE 610 620 630 640 650 660 LACDSQEDVD SWKASLLRAG VYPDKSVTEN DENGQAENFS MDPQLERQVE TIRNLVDSYM 670 680 690 700 710 720 SIINKCIRDL IPKTIMHLMI NNVKDFINSE LLAQLYSSED QNTLMEESAE QAQRRDEMLR 730 740 750 760 770 780 MYQALKEALA IIGDINTATV STPAPPPVDD SWLQHSRRSP PPSPTTQRRL TISAPLPRPT 790 800 810 820 830 840 SGRGPAPAIP SPGPHSGAPP VPFRPGPLPP FPNSSDSFGA PPQVPSRPTR APPSVPSRRP 850 PPSPTRPTII RPLESSLLD

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain, Cerebellum and Spinal cord.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed: 12693553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 394-863 (ISOFORM 1). Tissue: Brain.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK036199 mRNA. Translation: BAC29343.1. AK049724 mRNA. Translation: BAC33895.1. AK147678 mRNA. Translation: BAE28068.1. BC141143 mRNA. Translation: AAI41144.1. BC141144 mRNA. Translation: AAI41145.1. AK122379 Transcribed RNA. Translation: BAC65661.1.
IPI	IPI00227432. IPI00625738.
RefSeq	NP_001033708.1. NP_766234.1.
UniGene	Mm.441620
3D structure databases
HSSP	HSSP built from PDB template 2DYN based on UniProtKB Q05193.
SMR	Q8BZ98. Positions 6-304, 516-625.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BZ98.
PTM databases
PhosphoSite	Q8BZ98.
Proteomic databases
PRIDE	Q8BZ98.
Genome annotation databases
Ensembl	ENSMUST00000070330; ENSMUSP00000064538; ENSMUSG00000040265; Mus musculus. [Genome view] ENSMUST00000086074; ENSMUSP00000083241; ENSMUSG00000040265; Mus musculus. [Genome view]
GeneID	103967.
KEGG	mmu:103967.
UCSC	uc007dga.1. mouse. uc007dgb.1. mouse.
Organism-specific databases
CTD	103967.
MGI	MGI:1341299. Dnm3.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q8BZ98.
Enzyme and pathway databases
BRENDA	3.6.5.5. 244.
Gene expression databases
ArrayExpress	Q8BZ98.
Bgee	Q8BZ98.
CleanEx	MM_DNM3.
Genevestigator	Q8BZ98.
Family and domain databases
InterPro	IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	356261.
SOURCE	Search...

Entry information

Entry name

DYN3_MOUSE

Accession

Primary (citable) accession number: Q8BZ98
Secondary accession number(s): B2RUH0 , Q3UGY9, Q80TR2, Q8BWW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)