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Protein

Stonin-2

Gene

Ston2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in endocytic machinery. Involved in the synaptic vesicle recycling. May facilitate clathrin-coated vesicle uncoating (By similarity).By similarity

GO - Biological processi

  • chemical synaptic transmission Source: MGI
  • hematopoietic progenitor cell differentiation Source: MGI
  • regulation of endocytosis Source: UniProtKB
  • synaptic vesicle endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Stonin-2
Alternative name(s):
Stoned B
Gene namesi
Name:Ston2
Synonyms:Stn2, Stnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1918272. Ston2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • clathrin-coated vesicle Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: UniProtKB-SubCell
  • neuron projection Source: UniProtKB-SubCell
  • nucleolus Source: MGI
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857331 – 895Stonin-2Add BLAST895

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphothreonineCombined sources1
Modified residuei278PhosphoserineBy similarity1
Modified residuei299PhosphoserineBy similarity1
Modified residuei759PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in vitro by PKD.By similarity
Neddylated; deneddylated via its interaction with the COP9 signalosome (CSN) complex through TOR1A and COPS4.By similarity
Ubiquitinated; leading to its degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BZ60.
MaxQBiQ8BZ60.
PaxDbiQ8BZ60.
PRIDEiQ8BZ60.

PTM databases

iPTMnetiQ8BZ60.
PhosphoSitePlusiQ8BZ60.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020961.
CleanExiMM_STON2.
ExpressionAtlasiQ8BZ60. baseline and differential.

Interactioni

Subunit structurei

Interacts with the second C2 domain of synaptotagmins SYT1 and SYT2. Interacts with EPS15, EPS15R and ITSN1. Interacts indirectly with the AP-2 adapter complex. Interacts with TOR1A and COPS4; the interaction controls STON2 protein stability (By similarity).By similarity

Protein-protein interaction databases

BioGridi224423. 1 interactor.
IntActiQ8BZ60. 3 interactors.
STRINGi10090.ENSMUSP00000053908.

Structurei

3D structure databases

ProteinModelPortaliQ8BZ60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini424 – 557SHDPROSITE-ProRule annotationAdd BLAST134
Domaini565 – 872MHDPROSITE-ProRule annotationAdd BLAST308

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi310 – 312NPF 13
Motifi326 – 328NPF 23

Domaini

The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of SYT1, SYT2, EPS15, EPS15R and ITSN1.By similarity

Sequence similaritiesi

Belongs to the Stoned B family.Curated
Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation
Contains 1 SHD (stonin homology) domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2677. Eukaryota.
ENOG410XQHF. LUCA.
GeneTreeiENSGT00830000128302.
HOGENOMiHOG000236337.
HOVERGENiHBG097637.
InParanoidiQ8BZ60.
KOiK20067.
PhylomeDBiQ8BZ60.
TreeFamiTF300393.

Family and domain databases

InterProiIPR028565. MHD.
IPR012320. SHD.
IPR031228. STON2.
IPR017110. Stonin.
IPR022699. Stonin2_N.
[Graphical view]
PANTHERiPTHR10529:SF246. PTHR10529:SF246. 2 hits.
PfamiPF00928. Adap_comp_sub. 1 hit.
PF12016. Stonin2_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037099. Stonin. 1 hit.
SUPFAMiSSF49447. SSF49447. 2 hits.
PROSITEiPS51072. MHD. 1 hit.
PS51070. SHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BZ60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLDHVIAT HQSEWVSFSE EPLFPTPLEG GTEEHFPGLS SSSERSESSS
60 70 80 90 100
GENHVVDEGS QDLSHSEQDD SSEKMGLISE AASPPGSPVQ PTPDLASAIS
110 120 130 140 150
NWVQFEDDTP WSSTSPPHKE TALTLTMPCW TCPSFDSLRR CPLTSESSWT
160 170 180 190 200
THSEDTSSPS VAPSYTDLQL INTEEQASGR ASGTDSTDNS SSLQEDEEVE
210 220 230 240 250
MEAISWWAGS PAMNGHPAAP PVTTARFPSW VTFEDNEVGC PSPPVPSPKK
260 270 280 290 300
PNTPSAATAA PDVPFNSTGS FKRDRPKSTL MNLPKVQKLD ISSLNRPPSV
310 320 330 340 350
IEAPPWRATN PFLNETLQDV QPSPINPFSA FFEEQERRSQ NSSVSSTTGK
360 370 380 390 400
SQRDSLIVVY QDAISFDDSG KSQPHPDAIE KLKQLQIDDP DPVGNTALPD
410 420 430 440 450
DDPTASVELD APSPASALSQ PRDGWPMMLR IPEKKNIMSS RHWGPIYIKL
460 470 480 490 500
TASGYLQLYY EQGLEKPFRE FKLEICHEVS EPRLQNYDEN GRIHSLRIDR
510 520 530 540 550
VTYKEKKKYQ PKPAVAHAAE REQVIKLGTT NYDDFRSFIH AVQDRLMDLP
560 570 580 590 600
VLSMDLSTVG LNYLEEEITV DVRDEFSGTV GKGDNQILQH HVLTRIHILS
610 620 630 640 650
FLSGLAECRL GLNDILIKGN EIVSRQDIMP TTTTKWIKLH ECRFHGCVDE
660 670 680 690 700
DVFNSSRVIL FNPLDACRFE LMRFRTVFAE KTLPFTLRTA ASINGAEVEV
710 720 730 740 750
QSWLRMSPGF SSNRDPLTQV PCENVMVRYP VPSEWVKNFR RDSVLGEKSL
760 770 780 790 800
KAKVNRGASF GSAGASGSEP VMRVTLGTAK YEHAFNSIVW RINRLPDKNS
810 820 830 840 850
ASGHPHCFFC HLELGSDREV PSRFANYVNV EFSMPTTSAS KAAVRSVSVE
860 870 880 890
DKPDVRKWVN YSAHYSYKVE IEQKKSLKPD FEGEDLENPK ECGVQ
Length:895
Mass (Da):99,611
Last modified:March 1, 2003 - v1
Checksum:i2CA089956349B96F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036612 mRNA. Translation: BAC29507.1.
BC132531 mRNA. Translation: AAI32532.1.
BC138054 mRNA. Translation: AAI38055.1.
CCDSiCCDS26090.1.
RefSeqiNP_780576.1. NM_175367.6.
XP_006515436.1. XM_006515373.3.
XP_006515437.1. XM_006515374.3.
XP_011242284.1. XM_011243982.2.
UniGeneiMm.23125.

Genome annotation databases

EnsembliENSMUST00000052969; ENSMUSP00000053908; ENSMUSG00000020961.
GeneIDi108800.
KEGGimmu:108800.
UCSCiuc007okt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036612 mRNA. Translation: BAC29507.1.
BC132531 mRNA. Translation: AAI32532.1.
BC138054 mRNA. Translation: AAI38055.1.
CCDSiCCDS26090.1.
RefSeqiNP_780576.1. NM_175367.6.
XP_006515436.1. XM_006515373.3.
XP_006515437.1. XM_006515374.3.
XP_011242284.1. XM_011243982.2.
UniGeneiMm.23125.

3D structure databases

ProteinModelPortaliQ8BZ60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224423. 1 interactor.
IntActiQ8BZ60. 3 interactors.
STRINGi10090.ENSMUSP00000053908.

PTM databases

iPTMnetiQ8BZ60.
PhosphoSitePlusiQ8BZ60.

Proteomic databases

EPDiQ8BZ60.
MaxQBiQ8BZ60.
PaxDbiQ8BZ60.
PRIDEiQ8BZ60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052969; ENSMUSP00000053908; ENSMUSG00000020961.
GeneIDi108800.
KEGGimmu:108800.
UCSCiuc007okt.2. mouse.

Organism-specific databases

CTDi85439.
MGIiMGI:1918272. Ston2.

Phylogenomic databases

eggNOGiKOG2677. Eukaryota.
ENOG410XQHF. LUCA.
GeneTreeiENSGT00830000128302.
HOGENOMiHOG000236337.
HOVERGENiHBG097637.
InParanoidiQ8BZ60.
KOiK20067.
PhylomeDBiQ8BZ60.
TreeFamiTF300393.

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiSton2. mouse.
PROiQ8BZ60.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020961.
CleanExiMM_STON2.
ExpressionAtlasiQ8BZ60. baseline and differential.

Family and domain databases

InterProiIPR028565. MHD.
IPR012320. SHD.
IPR031228. STON2.
IPR017110. Stonin.
IPR022699. Stonin2_N.
[Graphical view]
PANTHERiPTHR10529:SF246. PTHR10529:SF246. 2 hits.
PfamiPF00928. Adap_comp_sub. 1 hit.
PF12016. Stonin2_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037099. Stonin. 1 hit.
SUPFAMiSSF49447. SSF49447. 2 hits.
PROSITEiPS51072. MHD. 1 hit.
PS51070. SHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTON2_MOUSE
AccessioniPrimary (citable) accession number: Q8BZ60
Secondary accession number(s): A2RTJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.