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Protein

Zinc finger protein 609

Gene

Znf609

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor, which activates RAG1, and possibly RAG2, transcription. Through the regulation of RAG1/2 expression, may regulate thymocyte maturation (PubMed:28344082). Along with NIPBL and the multiprotein complex Integrator, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (PubMed:28041881).2 Publications
Isoform 2: Involved in regulation of myoblast proliferation during myogenesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri495 – 520C2H2-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • muscle organ development Source: UniProtKB-KW
  • positive regulation of neuron migration Source: UniProtKB
  • positive regulation of T cell differentiation in thymus Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of myoblast proliferation Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein
Biological processMyogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 609
Gene namesi
Name:Znf609
Synonyms:Kiaa0295, Zfp609
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2674092. Zfp609.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002804231 – 1413Zinc finger protein 609Add BLAST1413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei358PhosphoserineCombined sources1
Modified residuei361PhosphoserineCombined sources1
Modified residuei379PhosphoserineBy similarity1
Modified residuei381PhosphothreonineBy similarity1
Modified residuei413PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei452PhosphoserineBy similarity1
Modified residuei467PhosphoserineCombined sources1
Modified residuei470PhosphoserineBy similarity1
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei533PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei577PhosphoserineCombined sources1
Modified residuei742PhosphoserineBy similarity1
Modified residuei745PhosphothreonineBy similarity1
Modified residuei757PhosphoserineBy similarity1
Cross-linki788Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei803PhosphoserineBy similarity1
Modified residuei822PhosphothreonineBy similarity1
Modified residuei841PhosphoserineBy similarity1
Modified residuei845PhosphoserineCombined sources1
Modified residuei848PhosphoserineCombined sources1
Modified residuei1057PhosphoserineCombined sources1
Cross-linki1063Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1155Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BZ47.
MaxQBiQ8BZ47.
PaxDbiQ8BZ47.
PRIDEiQ8BZ47.

PTM databases

iPTMnetiQ8BZ47.
PhosphoSitePlusiQ8BZ47.

Expressioni

Tissue specificityi

Expressed in myoblasts (PubMed:28344082). Expressed in neurons in various brain regions, including striatum, prefrontal cortex, olfactory bulb, midbrain, cerebellum and hippocampus (PubMed:25921068). Expressed in neural stem cells (at protein level) (PubMed:28041881). Expressed in thymocytes (PubMed:23076336).4 Publications

Developmental stagei

Expressed in the developing brain at 12.5 dpc. At 14.5 dpc, becomes enriched in the ventricular zone and later, restricted to the progenitor population as cortical neurogenesis peaks. At this stage, not detected in the subventricular zone/intermediate zone (SVZ/IZ). At later stages, detected in the neurons of the cortical plate and in stem cells near the ventricular surface.1 Publication

Inductioni

Down-regulated by ZNF608 (PubMed:23076336). Isoform 2 is up-regulated during neuronal differentiation (PubMed:25921068).2 Publications

Gene expression databases

BgeeiENSMUSG00000040524.
CleanExiMM_ZFP609.
ExpressionAtlasiQ8BZ47. baseline and differential.

Interactioni

Subunit structurei

Interacts (via N-terminus) with NIPBL (PubMed:28041881). Interacts with the multiprotein complex Integrator (PubMed:28041881).1 Publication

Protein-protein interaction databases

BioGridi229567. 2 interactors.
IntActiQ8BZ47. 1 interactor.
STRINGi10090.ENSMUSP00000124089.

Structurei

3D structure databases

ProteinModelPortaliQ8BZ47.
SMRiQ8BZ47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi378 – 460Ser-richAdd BLAST83
Compositional biasi724 – 734Poly-LysAdd BLAST11
Compositional biasi955 – 1020Gln-richAdd BLAST66
Compositional biasi1335 – 1338Poly-Gly4
Compositional biasi1368 – 1373Poly-His6

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri495 – 520C2H2-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IQYG. Eukaryota.
ENOG410XRNB. LUCA.
GeneTreeiENSGT00390000008748.
HOGENOMiHOG000082539.
HOVERGENiHBG054557.
InParanoidiQ8BZ47.
OMAiLMSTHHH.
OrthoDBiEOG091G00Q9.
PhylomeDBiQ8BZ47.
TreeFamiTF329775.

Family and domain databases

InterProiView protein in InterPro
IPR013087. Znf_C2H2_type.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZ47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSSGACGG KGVDANPVET YDSGDEWDIG VGNLIIDLDA DLEKDQQKLE
60 70 80 90 100
MSGSKEVGIP APNAVATLPD NIKFVTPVPG PQGKEGKSKS KRSKSGKDAS
110 120 130 140 150
KPTPGTSLFS PSEGAASKKE VQGRAGDGAS AGGLVAAVAP KGSEKAAKAS
160 170 180 190 200
RSVAGSKKEK ENSSSKGKKE RSEGVGTCSE KDPGVLQPVP LGGRGSQYDG
210 220 230 240 250
SAGMDTGTVE PLGSIAIEPG AALNPLGTKP EPEEGENECR PLKKVKSEKM
260 270 280 290 300
ESPVSTPAVL PLHLLVPVVN NDISSPCEQI MVRTRSVGVN TCDVALATEP
310 320 330 340 350
ECLGPCEPGT SVNLEGIVWQ ETEDGMLVVN VTWRNKTYVG TLLDCTRHDW
360 370 380 390 400
APPRFCDSPT SDLEMRNGRG RGKRMRPSSN TPVSEAAAAS DSKGTSSSSK
410 420 430 440 450
TRAGANSKGR RGSQNSSEHR PPASSTSEDV KASPSSANKR KSKPLSDMEL
460 470 480 490 500
NSSSEDSKGS KRVRTNSMGS ATGPLPGTKV EPTLVDRNCP SPVLIDCPHP
510 520 530 540 550
NCNKKYKHIN GLKYHQAHAH TDDDSKPEAD GDSEYGEEPA LHADLSCNGA
560 570 580 590 600
PVPQKGSLSP ARSATPKVRL VEPHSPSPSS KFSTKGLCKK KLSGEGDTDP
610 620 630 640 650
GALSNDGSDD GPSVMDETSN DAFDSLERKC MEKEKCKKPS SLKSEKIPSK
660 670 680 690 700
SLKSARPIAP AIPPQQIYTF QTATFTAASP GSSSGLTTTV VQAMPNSPQL
710 720 730 740 750
KPIQPKPTVM GEPFTVNPAL TPAKDKKKKD KKKKDSSKEL ESPLTPGKVC
760 770 780 790 800
RAEEGKSPFR DAAGDGIKVE SLLNGSSESH QSRLASIKAE ADKIYSFTDN
810 820 830 840 850
APSPSIGGSS RLDSTTPTQP LTPLHVVTQN GAEASSVKTN SPAYSDISDA
860 870 880 890 900
GEDGEGKVDS AKSKDPEQLV KEGAKKTLFP PQPQSKDSPY YQGFESYYSP
910 920 930 940 950
GYAQSSPGTL TSSSQAGMEG QPLKTKKDEE PESVEGKVKN DVCEEKKPEL
960 970 980 990 1000
SNSSQQPSVI QQRPNMYMQS LYYNQYAYVP PYGYSDQSYH SHLLSTNTAY
1010 1020 1030 1040 1050
RQQYEEQQKR QSLEQQQQQQ RGLDKKTEMG LKEREASLKE EWKQKPSIPP
1060 1070 1080 1090 1100
TLTKAPSLTD LVKSGPGKAK EPGTDPAKSV IIPKLDDSSK LPSQPPEGLK
1110 1120 1130 1140 1150
GKLGEASHLG KEASEAKTGT ECGRQAEVDP ILWYRQETES RMWTYVYPAK
1160 1170 1180 1190 1200
YSDIKSEDDR WKEERDRKLK EDRSRSKDSV PKEDGKESTS SDCKLPPSEE
1210 1220 1230 1240 1250
SRLGSKEPRP SVHVPVSSPL TQHQSYIPYM HGYSYSQSYD PNHPSYRGMP
1260 1270 1280 1290 1300
AVMMQNYPGS YLPSSYSFSP YGSKVSGGED ADKARASPSV SCKASSESKA
1310 1320 1330 1340 1350
LDILQQHASH YKSKSPTISD KNSQERDRGG CGVVGGGGSC GSVAGAGGTD
1360 1370 1380 1390 1400
RSADRPRTSP SQRLMSTHHH HHHLGYSLLP AQYNLPYAAG LSSTAIVASQ
1410
QGSTPSLYPP PRR
Length:1,413
Mass (Da):151,117
Last modified:March 20, 2007 - v2
Checksum:i6BC74B23591605FF
GO
Isoform 2 (identifier: Q8BZ47-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-250: M → Q
     251-1413: Missing.

Note: Produced by a back-splicing reaction which joins the 5'-splice site of the first coding exon with the 3'-splice site of the upstream intron resulting in a circular RNA, called circ-ZNF609. The translation starts with the same initiator methionine as that of the linear transcript encoding isoform 1. The stop codon is created upon circularization.1 Publication
Show »
Length:250
Mass (Da):25,358
Checksum:iB803E54807D8B3DB
GO

Sequence cautioni

The sequence BAC29549 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti697S → G in BAC29549 (PubMed:16141072).Curated1
Sequence conflicti735D → K in AAH16271 (PubMed:15489334).Curated1
Sequence conflicti914S → N in BAC97921 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059084250M → Q in isoform 2. 1
Alternative sequenceiVSP_059085251 – 1413Missing in isoform 2. Add BLAST1163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151906 Genomic DNA. No translation available.
AC121979 Genomic DNA. No translation available.
BC016271 mRNA. Translation: AAH16271.1.
BC058943 mRNA. Translation: AAH58943.1. Sequence problems.
AK036715 mRNA. Translation: BAC29549.1. Different initiation.
AK129111 mRNA. Translation: BAC97921.1.
CCDSiCCDS23297.2. [Q8BZ47-1]
RefSeqiNP_766124.2. NM_172536.3. [Q8BZ47-1]
UniGeneiMm.139231.

Genome annotation databases

EnsembliENSMUST00000159109; ENSMUSP00000124089; ENSMUSG00000040524. [Q8BZ47-1]
GeneIDi214812.
KEGGimmu:214812.
UCSCiuc009qdw.2. mouse. [Q8BZ47-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZN609_MOUSE
AccessioniPrimary (citable) accession number: Q8BZ47
Secondary accession number(s): Q05DD7, Q6PD47, Q6ZQE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: November 22, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot