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Q8BYW9

- EOGT_MOUSE

UniProt

Q8BYW9 - EOGT_MOUSE

Protein

EGF domain-specific O-linked N-acetylglucosamine transferase

Gene

Eogt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.1 Publication

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine.1 Publication
    UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.1 Publication

    GO - Molecular functioni

    1. protein N-acetylglucosaminyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. metabolic process Source: UniProtKB
    2. protein O-linked glycosylation Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Protein family/group databases

    CAZyiGT61. Glycosyltransferase Family 61.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.255)
    Alternative name(s):
    Extracellular O-linked N-acetylglucosamine transferase
    Gene namesi
    Name:Eogt
    Synonyms:Aer61, Eogt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:2141669. Eogt.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 527508EGF domain-specific O-linked N-acetylglucosamine transferasePRO_0000301972Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BYW9.
    PaxDbiQ8BYW9.
    PRIDEiQ8BYW9.

    PTM databases

    PhosphoSiteiQ8BYW9.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in brain, heart, kidney, lung, skeletal muscles and thymus. Highest expression is observed in lung and the lowest in skeletal muscles.1 Publication

    Developmental stagei

    Expressed at embryonic day (E) 10.5 in the growing edge of the limb buds. At E11.5, enriched in the apical ectodermal ridge of the limbs. By E12.5, expression assumes a digit-condensation pattern in the 4 limbs.1 Publication

    Gene expression databases

    ArrayExpressiQ8BYW9.
    BgeeiQ8BYW9.
    CleanExiMM_A130022J15RIK.
    GenevestigatoriQ8BYW9.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000061610.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi295 – 2973Required for optimal activityBy similarity
    Motifi524 – 5274Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyltransferase 61 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG320328.
    GeneTreeiENSGT00750000117715.
    HOGENOMiHOG000033829.
    HOVERGENiHBG056678.
    InParanoidiQ8BYW9.
    KOiK18134.
    OMAiDSKRVCF.
    OrthoDBiEOG7D2FDQ.
    PhylomeDBiQ8BYW9.
    TreeFamiTF313716.

    Family and domain databases

    InterProiIPR007657. Glycosyltransferase_AER61.
    [Graphical view]
    PfamiPF04577. DUF563. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BYW9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMLLVFGVL LHEVPLSGQD KAHSEADDAP GKALYDYSSL RLPAEHIPFF    50
    LHNNRHVASV CREDSHCPYK KHLENLNYCW GYEKSCAPEF RFGSPVCSYV 100
    DLGWTDTLES AQDMFWRQAD FGYARERLGE IRTICQPERA SDSSLVCSRY 150
    LQYCRATGLY LDLRNIKRNH DRFKEDFLQG GEIGGYCKLD SHALVSEGQR 200
    KSPLQSWFAE LQGYTQLNFR PIEDAKCDIV VEKPTYFMKL DAGINMYHHF 250
    CDFLNLYLTQ HVNNSFSTDV YIVMWDTSTY GYGDLFSDTW KAFTDYDVIH 300
    LKTYDSKKVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL 350
    HRLNITQEGP KDGKVRVTIL ARSTEYRKIL NQDELVNALK TVSTFEVRVV 400
    DYKYRELGFL DQLRITHNTD IFIGMHGAGL THLLFLPDWA AVFELYNCED 450
    ERCYLDLARL RGIHYITWRK PSKVFPQDKG HHPTLGEHPK FTNYSFDVEE 500
    FMYLVLQAAE HVLQHPQWPF KKKHDEL 527
    Length:527
    Mass (Da):61,446
    Last modified:March 1, 2003 - v1
    Checksum:i793D0E8F481AD5E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK037507 mRNA. Translation: BAC29821.1.
    BC048939 mRNA. Translation: AAH48939.1.
    CCDSiCCDS20383.1.
    RefSeqiNP_780522.1. NM_175313.4.
    XP_006505321.1. XM_006505258.1.
    XP_006505322.1. XM_006505259.1.
    UniGeneiMm.251789.
    Mm.486365.

    Genome annotation databases

    EnsembliENSMUST00000054344; ENSMUSP00000061610; ENSMUSG00000035245.
    GeneIDi101351.
    KEGGimmu:101351.
    UCSCiuc009dai.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK037507 mRNA. Translation: BAC29821.1 .
    BC048939 mRNA. Translation: AAH48939.1 .
    CCDSi CCDS20383.1.
    RefSeqi NP_780522.1. NM_175313.4.
    XP_006505321.1. XM_006505258.1.
    XP_006505322.1. XM_006505259.1.
    UniGenei Mm.251789.
    Mm.486365.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000061610.

    Protein family/group databases

    CAZyi GT61. Glycosyltransferase Family 61.

    PTM databases

    PhosphoSitei Q8BYW9.

    Proteomic databases

    MaxQBi Q8BYW9.
    PaxDbi Q8BYW9.
    PRIDEi Q8BYW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054344 ; ENSMUSP00000061610 ; ENSMUSG00000035245 .
    GeneIDi 101351.
    KEGGi mmu:101351.
    UCSCi uc009dai.1. mouse.

    Organism-specific databases

    CTDi 285203.
    MGIi MGI:2141669. Eogt.

    Phylogenomic databases

    eggNOGi NOG320328.
    GeneTreei ENSGT00750000117715.
    HOGENOMi HOG000033829.
    HOVERGENi HBG056678.
    InParanoidi Q8BYW9.
    KOi K18134.
    OMAi DSKRVCF.
    OrthoDBi EOG7D2FDQ.
    PhylomeDBi Q8BYW9.
    TreeFami TF313716.

    Miscellaneous databases

    NextBioi 354880.
    PROi Q8BYW9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BYW9.
    Bgeei Q8BYW9.
    CleanExi MM_A130022J15RIK.
    Genevestigatori Q8BYW9.

    Family and domain databases

    InterProi IPR007657. Glycosyltransferase_AER61.
    [Graphical view ]
    Pfami PF04577. DUF563. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    3. "O-linked-N-acetylglucosamine modification of mammalian Notch receptors by an atypical O-GlcNAc transferase Eogt1."
      Sakaidani Y., Ichiyanagi N., Saito C., Nomura T., Ito M., Nishio Y., Nadano D., Matsuda T., Furukawa K., Okajima T.
      Biochem. Biophys. Res. Commun. 419:14-19(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    4. Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiEOGT_MOUSE
    AccessioniPrimary (citable) accession number: Q8BYW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3