Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rho GTPase-activating protein 25

Gene

Arhgap25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 25
Alternative name(s):
Rho-type GTPase-activating protein 25
Gene namesi
Name:Arhgap25
Synonyms:Kiaa0053
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2443687. Arhgap25.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Rho GTPase-activating protein 25PRO_0000056717Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei363 – 3631PhosphoserineCombined sources
Modified residuei396 – 3961PhosphoserineCombined sources
Modified residuei403 – 4031PhosphoserineCombined sources
Modified residuei407 – 4071PhosphothreonineCombined sources
Modified residuei537 – 5371PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BYW1.
MaxQBiQ8BYW1.
PaxDbiQ8BYW1.
PRIDEiQ8BYW1.

PTM databases

iPTMnetiQ8BYW1.
PhosphoSiteiQ8BYW1.

Expressioni

Gene expression databases

BgeeiQ8BYW1.
CleanExiMM_ARHGAP25.
GenevisibleiQ8BYW1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109267.

Structurei

3D structure databases

ProteinModelPortaliQ8BYW1.
SMRiQ8BYW1. Positions 47-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 151106PHPROSITE-ProRule annotationAdd
BLAST
Domaini160 – 354195Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili540 – 641102Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOVERGENiHBG058875.
InParanoidiQ8BYW1.
OMAiCLEDKAS.
OrthoDBiEOG79W94M.
PhylomeDBiQ8BYW1.
TreeFamiTF323577.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BYW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKLPRNWD FNLKAEASKI ARSRSVMTGE QMAAFHPPTT PNPLERPIKV
60 70 80 90 100
GWLKKQRSIV KNWQQRYFVL RAQQLYYYKD EEDSKPQGCM YLPGSTVKEI
110 120 130 140 150
ATNPEEAGKF VFEVIPASSD QNRIGQDSYV LMASSQVEME EWVKFLRRVA
160 170 180 190 200
GTPSGAVFGQ RLDETVAYEQ KFGPHLVPIL VEKCAEFILE HGVSEEGIFR
210 220 230 240 250
LPGQDNLVKQ LRDAFDAGER PSFDRDTDVH TVASLLKLYL RDLPEPVVPW
260 270 280 290 300
SQYEGFLLCG QLMNADEAKA QQELVKQLST LPRDNYNLLS YICRFLHEIQ
310 320 330 340 350
LNCAVNKMSV DNLATVIGVN LIRSKVEDPA VIMRGTPQIQ RVMTMMIRDH
360 370 380 390 400
EVLFPKSKDA PISPPAQKND AKKAPVPRSS VGWDATEDPP LSRTDSFSNT
410 420 430 440 450
ASSPDATSPT GPLPSDQHQE DSGKAPRENP GDWKMQSRKR TQTLPNRKCF
460 470 480 490 500
LTSAFQGTTS SKLEIFKNEF WSPSSEAKAG EGHRRTMSQD LRHLSNDQRT
510 520 530 540 550
STYDNVPTSP QSQGNPAGAL SPPASDSKRD ALVSTDSEME AGSKNSGEDD
560 570 580 590 600
LDSLQRTVQS LQKEIETQKQ VYEEQIKNLE KENYDVWAKV VRLNEELERE
610 620 630 640
RKKFAALEIS LRNVERSRED VEKRNRVLEE EVKEFVKSME KPKTKTDP
Note: No experimental confirmation available.
Length:648
Mass (Da):73,383
Last modified:May 10, 2004 - v2
Checksum:iCBDFE75515BB2C51
GO
Isoform 2 (identifier: Q8BYW1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.

Note: No experimental confirmation available.
Show »
Length:559
Mass (Da):62,874
Checksum:i0FE0BA9CA2E45631
GO
Isoform 3 (identifier: Q8BYW1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:622
Mass (Da):70,382
Checksum:i61449F16B50281B2
GO

Sequence cautioni

The sequence BAC29857.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441K → N in AAI08401 (PubMed:15489334).Curated
Sequence conflicti333 – 3331M → T in BAE41444 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8989Missing in isoform 2. 1 PublicationVSP_019233Add
BLAST
Alternative sequencei1 – 2626Missing in isoform 3. 2 PublicationsVSP_019234Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037710 mRNA. Translation: BAC29857.1. Different initiation.
AK137444 mRNA. Translation: BAE23353.1.
AK159941 mRNA. Translation: BAE35498.1.
AK159598 mRNA. Translation: BAE35218.1.
AK169897 mRNA. Translation: BAE41444.1.
BC108400 mRNA. Translation: AAI08401.1.
BC138751 mRNA. Translation: AAI38752.1.
AK172878 mRNA. Translation: BAD32156.1.
CCDSiCCDS20321.1. [Q8BYW1-1]
CCDS20322.1. [Q8BYW1-3]
CCDS71790.1. [Q8BYW1-2]
RefSeqiNP_001032816.1. NM_001037727.2. [Q8BYW1-1]
NP_001273539.1. NM_001286610.1. [Q8BYW1-2]
NP_780685.2. NM_175476.4. [Q8BYW1-3]
UniGeneiMm.119564.

Genome annotation databases

EnsembliENSMUST00000071024; ENSMUSP00000068964; ENSMUSG00000030047. [Q8BYW1-2]
ENSMUST00000101197; ENSMUSP00000098758; ENSMUSG00000030047. [Q8BYW1-3]
ENSMUST00000113637; ENSMUSP00000109267; ENSMUSG00000030047. [Q8BYW1-1]
GeneIDi232201.
KEGGimmu:232201.
UCSCiuc009ctl.2. mouse. [Q8BYW1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037710 mRNA. Translation: BAC29857.1. Different initiation.
AK137444 mRNA. Translation: BAE23353.1.
AK159941 mRNA. Translation: BAE35498.1.
AK159598 mRNA. Translation: BAE35218.1.
AK169897 mRNA. Translation: BAE41444.1.
BC108400 mRNA. Translation: AAI08401.1.
BC138751 mRNA. Translation: AAI38752.1.
AK172878 mRNA. Translation: BAD32156.1.
CCDSiCCDS20321.1. [Q8BYW1-1]
CCDS20322.1. [Q8BYW1-3]
CCDS71790.1. [Q8BYW1-2]
RefSeqiNP_001032816.1. NM_001037727.2. [Q8BYW1-1]
NP_001273539.1. NM_001286610.1. [Q8BYW1-2]
NP_780685.2. NM_175476.4. [Q8BYW1-3]
UniGeneiMm.119564.

3D structure databases

ProteinModelPortaliQ8BYW1.
SMRiQ8BYW1. Positions 47-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109267.

PTM databases

iPTMnetiQ8BYW1.
PhosphoSiteiQ8BYW1.

Proteomic databases

EPDiQ8BYW1.
MaxQBiQ8BYW1.
PaxDbiQ8BYW1.
PRIDEiQ8BYW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071024; ENSMUSP00000068964; ENSMUSG00000030047. [Q8BYW1-2]
ENSMUST00000101197; ENSMUSP00000098758; ENSMUSG00000030047. [Q8BYW1-3]
ENSMUST00000113637; ENSMUSP00000109267; ENSMUSG00000030047. [Q8BYW1-1]
GeneIDi232201.
KEGGimmu:232201.
UCSCiuc009ctl.2. mouse. [Q8BYW1-1]

Organism-specific databases

CTDi9938.
MGIiMGI:2443687. Arhgap25.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOVERGENiHBG058875.
InParanoidiQ8BYW1.
OMAiCLEDKAS.
OrthoDBiEOG79W94M.
PhylomeDBiQ8BYW1.
TreeFamiTF323577.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ8BYW1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BYW1.
CleanExiMM_ARHGAP25.
GenevisibleiQ8BYW1. MM.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Bone, Dendritic cell and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 117-648 (ISOFORMS 1/2/3).
    Tissue: Jaw, Limb and Lung.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 100-648 (ISOFORMS 1/2/3).
    Tissue: Thymus.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-363 AND THR-407, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396; SER-403 AND SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung and Spleen.

Entry informationi

Entry nameiRHG25_MOUSE
AccessioniPrimary (citable) accession number: Q8BYW1
Secondary accession number(s): B2RS69
, Q2VPR2, Q3TE04, Q3TVX0, Q3UVB7, Q6A0E0, Q8BX98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: June 8, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.