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Protein

Neuroligin-3

Gene

Nlgn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.1 Publication

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: GO_Central
  2. cell adhesion molecule binding Source: BHF-UCL
  3. neurexin family protein binding Source: BHF-UCL
  4. receptor activity Source: GO_Central

GO - Biological processi

  1. adult behavior Source: MGI
  2. axon extension Source: BHF-UCL
  3. learning Source: MGI
  4. long-term synaptic potentiation Source: CACAO
  5. metabolic process Source: GOC
  6. negative regulation of dendritic spine morphogenesis Source: CACAO
  7. negative regulation of excitatory postsynaptic membrane potential Source: CACAO
  8. neuron cell-cell adhesion Source: BHF-UCL
  9. oligodendrocyte differentiation Source: MGI
  10. positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  11. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  12. positive regulation of synapse assembly Source: CACAO
  13. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  14. positive regulation of synaptic vesicle clustering Source: CACAO
  15. postsynaptic membrane assembly Source: BHF-UCL
  16. presynaptic membrane assembly Source: BHF-UCL
  17. receptor-mediated endocytosis Source: BHF-UCL
  18. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  19. regulation of dendritic spine morphogenesis Source: BHF-UCL
  20. regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  21. regulation of inhibitory postsynaptic membrane potential Source: BHF-UCL
  22. regulation of long-term synaptic potentiation Source: BHF-UCL
  23. regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
  24. regulation of respiratory gaseous exchange by neurological system process Source: MGI
  25. regulation of synaptic transmission Source: MGI
  26. regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  27. regulation of terminal button organization Source: BHF-UCL
  28. rhythmic synaptic transmission Source: BHF-UCL
  29. social behavior Source: BHF-UCL
  30. synapse organization Source: MGI
  31. visual learning Source: MGI
  32. vocalization behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Protein family/group databases

MEROPSiS09.987.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroligin-3
Alternative name(s):
Gliotactin homolog
Gene namesi
Name:Nlgn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2444609. Nlgn3.

Subcellular locationi

  1. Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
  2. Cell junctionsynapse 1 Publication

  3. Note: Detected at both glutamatergic and GABAergic synapses.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini35 – 686652ExtracellularSequence AnalysisAdd
BLAST
Transmembranei687 – 70721HelicalSequence AnalysisAdd
BLAST
Topological domaini708 – 825118CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell surface Source: BHF-UCL
  3. endocytic vesicle Source: BHF-UCL
  4. excitatory synapse Source: BHF-UCL
  5. integral component of plasma membrane Source: BHF-UCL
  6. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype, but mice present subtle behavorial changes with reduced ultrasound vocalization and impaired response to olfactory cues. In addition, mice have reduced brain volume. Mice lacking both NLGN1 and NLGN3, or NLGN2 and NLGN3, are viable, but have impaired breathing, drastically reduced reproduction rates and striking deficits in raising their offspring. Mice lacking NLGN1, NLGN2 and NLGN3 are born at the expected Mendelian rate, but die shortly after birth due to respiratory failure. They do not show a significant change in the number of synapses, but synapse function is strongly impaired.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence AnalysisAdd
BLAST
Chaini35 – 825791Neuroligin-3PRO_0000008646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi103 ↔ 138By similarity
Disulfide bondi317 ↔ 328By similarity
Disulfide bondi487 ↔ 521By similarity
Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence Analysis
Modified residuei769 – 7691Phosphotyrosine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BYM5.
PaxDbiQ8BYM5.
PRIDEiQ8BYM5.

PTM databases

PhosphoSiteiQ8BYM5.

Expressioni

Tissue specificityi

Brain and arteries (at protein level). Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Expressed in olfactory bulb and olfactory epithelium. Found in olfactory ensheathing glia but not in olfactory neurons, and in developing peripheral glia.5 Publications

Developmental stagei

Detected at embryonic day E17 and postnatal day P1 in retinal astrocytes, spinal chord astrocytes and Schwann cells of the dorsal root ganglion.1 Publication

Gene expression databases

BgeeiQ8BYM5.
CleanExiMM_NLGN3.
ExpressionAtlasiQ8BYM5. baseline and differential.
GenevestigatoriQ8BYM5.

Interactioni

Subunit structurei

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) (By similarity). Homodimer, and heterodimer with NLGN1 and NLGN2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BYM5. 2 interactions.
MINTiMINT-4103912.

Structurei

3D structure databases

ProteinModelPortaliQ8BYM5.
SMRiQ8BYM5. Positions 39-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ8BYM5.
KOiK07378.
TreeFamiTF326187.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030024. NLGN3.
[Graphical view]
PANTHERiPTHR11559:SF145. PTHR11559:SF145. 1 hit.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BYM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLQPSLSLS PTPTVGRSLC LTLGFLSLVL RASTQAPAPT VNTHFGKLRG
60 70 80 90 100
ARVPLPSEIL GPVDQYLGVP YAAPPIGEKR FLPPEPPPSW SGIRNATHFP
110 120 130 140 150
PVCPQNIHTA VPEVMLPVWF TANLDIVATY IQEPNEDCLY LNVYVPTEDG
160 170 180 190 200
SGAKKQGEDL ADNDGDEDED IRDSGAKPVM VYIHGGSYME GTGNMIDGSV
210 220 230 240 250
LASYGNVIVI TLNYRVGVLG FLSTGDQAAK GNYGLLDQIQ ALRWVSENIA
260 270 280 290 300
FFGGDPRRIT VFGSGIGASC VSLLTLSHHS EGLFQRAIIQ SGSALSSWAV
310 320 330 340 350
NYQPVKYTSL LADKVGCNVL DTVDMVDCLR QKSAKELVEQ DIQPARYHVA
360 370 380 390 400
FGPVIDGDVI PDDPEILMEQ GEFLNYDIML GVNQGEGLKF VEGVVDPEDG
410 420 430 440 450
VSGTDFDYSV SNFVDNLYGY PEGKDTLRET IKFMYTDWAD RDNPETRRKT
460 470 480 490 500
LVALFTDHQW VEPSVVTADL HARYGSPTYF YAFYHHCQSL MKPAWSDAAH
510 520 530 540 550
GDEVPYVFGV PMVGPTDLFP CNFSKNDVML SAVVMTYWTN FAKTGDPNKP
560 570 580 590 600
VPQDTKFIHT KANRFEEVAW SKYNPRDQLY LHIGLKPRVR DHYRATKVAF
610 620 630 640 650
WKHLVPHLYN LHDMFHYTST TTKVPPPDTT HSSHITRRPN GKTWSTKRPA
660 670 680 690 700
ISPAYSNENA PGSWNGDQDA GPLLVENPRD YSTELSVTIA VGASLLFLNV
710 720 730 740 750
LAFAALYYRK DKRRQEPLRQ PSPQRGTGAP ELGTAPEEEL AALQLGPTHH
760 770 780 790 800
ECEAGPPHDT LRLTALPDYT LTLRRSPDDI PLMTPNTITM IPNSLVGLQT
810 820
LHPYNTFAAG FNSTGLPHSH STTRV
Length:825
Mass (Da):91,162
Last modified:July 27, 2011 - v2
Checksum:iCEF160C63E0A71A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641D → E in BAC30207 (PubMed:16141072).Curated
Sequence conflicti459 – 4591Q → K in BAC31918 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039018 mRNA. Translation: BAC30207.1.
AK044438 mRNA. Translation: BAC31918.1.
AL683892 Genomic DNA. Translation: CAM24450.1.
CCDSiCCDS30313.1.
RefSeqiNP_766520.2. NM_172932.4.
UniGeneiMm.121508.

Genome annotation databases

EnsembliENSMUST00000065858; ENSMUSP00000066304; ENSMUSG00000031302.
GeneIDi245537.
KEGGimmu:245537.
UCSCiuc009txj.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039018 mRNA. Translation: BAC30207.1.
AK044438 mRNA. Translation: BAC31918.1.
AL683892 Genomic DNA. Translation: CAM24450.1.
CCDSiCCDS30313.1.
RefSeqiNP_766520.2. NM_172932.4.
UniGeneiMm.121508.

3D structure databases

ProteinModelPortaliQ8BYM5.
SMRiQ8BYM5. Positions 39-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BYM5. 2 interactions.
MINTiMINT-4103912.

Protein family/group databases

MEROPSiS09.987.

PTM databases

PhosphoSiteiQ8BYM5.

Proteomic databases

MaxQBiQ8BYM5.
PaxDbiQ8BYM5.
PRIDEiQ8BYM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065858; ENSMUSP00000066304; ENSMUSG00000031302.
GeneIDi245537.
KEGGimmu:245537.
UCSCiuc009txj.3. mouse.

Organism-specific databases

CTDi54413.
MGIiMGI:2444609. Nlgn3.

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ8BYM5.
KOiK07378.
TreeFamiTF326187.

Miscellaneous databases

NextBioi386794.
PROiQ8BYM5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BYM5.
CleanExiMM_NLGN3.
ExpressionAtlasiQ8BYM5. baseline and differential.
GenevestigatoriQ8BYM5.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030024. NLGN3.
[Graphical view]
PANTHERiPTHR11559:SF145. PTHR11559:SF145. 1 hit.
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hypothalamus and Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia."
    Gilbert M., Smith J., Roskams A.J., Auld V.J.
    Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
  5. "Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses."
    Budreck E.C., Scheiffele P.
    Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NLGN1 AND NLGN2, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-769, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. Cited for: TISSUE SPECIFICITY.
  8. "Neuroligin-3-deficient mice: model of a monogenic heritable form of autism with an olfactory deficit."
    Radyushkin K., Hammerschmidt K., Boretius S., Varoqueaux F., El-Kordi A., Ronnenberg A., Winter D., Frahm J., Fischer J., Brose N., Ehrenreich H.
    Genes Brain Behav. 8:416-425(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  9. "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions."
    Bottos A., Destro E., Rissone A., Graziano S., Cordara G., Assenzio B., Cera M.R., Mascia L., Bussolino F., Arese M.
    Proc. Natl. Acad. Sci. U.S.A. 106:20782-20787(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNLGN3_MOUSE
AccessioniPrimary (citable) accession number: Q8BYM5
Secondary accession number(s): A2AGI1, Q8BXR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.