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Q8BYM5 (NLGN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroligin-3
Alternative name(s):
Gliotactin homolog
Gene names
Name:Nlgn3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length825 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Ref.4

Subunit structure

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) By similarity. Homodimer, and heterodimer with NLGN1 and NLGN2 By similarity. Ref.5

Subcellular location

Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse. Note: Detected at both glutamatergic and GABAergic synapses. Ref.5

Tissue specificity

Brain and arteries (at protein level). Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Expressed in olfactory bulb and olfactory epithelium. Found in olfactory ensheathing glia but not in olfactory neurons, and in developing peripheral glia. Ref.3 Ref.4 Ref.5 Ref.7 Ref.9

Developmental stage

Detected at embryonic day E17 and postnatal day P1 in retinal astrocytes, spinal chord astrocytes and Schwann cells of the dorsal root ganglion. Ref.3

Disruption phenotype

No obvious phenotype, but mice present subtle behavorial changes with reduced ultrasound vocalization and impaired response to olfactory cues. In addition, mice have reduced brain volume. Mice lacking both NLGN1 and NLGN3, or NLGN2 and NLGN3, are viable, but have impaired breathing, drastically reduced reproduction rates and striking deficits in raising their offspring. Mice lacking NLGN1, NLGN2 and NLGN3 are born at the expected Mendelian rate, but die shortly after birth due to respiratory failure. They do not show a significant change in the number of synapses, but synapse function is strongly impaired. Ref.4 Ref.8

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon extension

Inferred from sequence or structural similarity. Source: BHF-UCL

neuron cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from mutant phenotype PubMed 21642956. Source: BHF-UCL

positive regulation of excitatory postsynaptic membrane potential

Inferred from mutant phenotype PubMed 21642956. Source: BHF-UCL

positive regulation of synapse assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic transmission, glutamatergic

Inferred from mutant phenotype PubMed 21642956. Source: BHF-UCL

postsynaptic membrane assembly

Inferred from mutant phenotype PubMed 21642956. Source: BHF-UCL

presynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

receptor-mediated endocytosis

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of N-methyl-D-aspartate selective glutamate receptor activity

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of dendritic spine morphogenesis

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of excitatory postsynaptic membrane potential

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of inhibitory postsynaptic membrane potential

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of long-term synaptic potentiation

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of respiratory gaseous exchange by neurological system process

Inferred from genetic interaction Ref.4. Source: MGI

regulation of synaptic transmission

Inferred from genetic interaction Ref.4. Source: MGI

regulation of synaptic transmission, glutamatergic

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

regulation of terminal button organization

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

rhythmic synaptic transmission

Inferred from sequence or structural similarity. Source: BHF-UCL

social behavior

Inferred from mutant phenotype PubMed 21808020. Source: BHF-UCL

synapse assembly

Inferred from Biological aspect of Ancestor. Source: RefGenome

synapse organization

Inferred from genetic interaction PubMed 21788371. Source: MGI

visual learning

Inferred from mutant phenotype PubMed 17823315. Source: MGI

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from sequence or structural similarity. Source: BHF-UCL

endocytic vesicle

Inferred from sequence or structural similarity. Source: BHF-UCL

excitatory synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

synapse

Inferred from sequence orthology PubMed 15681343. Source: MGI

   Molecular_functioncell adhesion molecule binding

Inferred from sequence or structural similarity. Source: BHF-UCL

neurexin family protein binding

Inferred from sequence or structural similarity. Source: BHF-UCL

receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Potential
Chain35 – 825791Neuroligin-3
PRO_0000008646

Regions

Topological domain35 – 686652Extracellular Potential
Transmembrane687 – 70721Helical; Potential
Topological domain708 – 825118Cytoplasmic Potential

Amino acid modifications

Modified residue7691Phosphotyrosine Ref.6
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Disulfide bond103 ↔ 138 By similarity
Disulfide bond317 ↔ 328 By similarity
Disulfide bond487 ↔ 521 By similarity

Experimental info

Sequence conflict641D → E in BAC30207. Ref.1
Sequence conflict4591Q → K in BAC31918. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BYM5 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: CEF160C63E0A71A4

FASTA82591,162
        10         20         30         40         50         60 
MWLQPSLSLS PTPTVGRSLC LTLGFLSLVL RASTQAPAPT VNTHFGKLRG ARVPLPSEIL 

        70         80         90        100        110        120 
GPVDQYLGVP YAAPPIGEKR FLPPEPPPSW SGIRNATHFP PVCPQNIHTA VPEVMLPVWF 

       130        140        150        160        170        180 
TANLDIVATY IQEPNEDCLY LNVYVPTEDG SGAKKQGEDL ADNDGDEDED IRDSGAKPVM 

       190        200        210        220        230        240 
VYIHGGSYME GTGNMIDGSV LASYGNVIVI TLNYRVGVLG FLSTGDQAAK GNYGLLDQIQ 

       250        260        270        280        290        300 
ALRWVSENIA FFGGDPRRIT VFGSGIGASC VSLLTLSHHS EGLFQRAIIQ SGSALSSWAV 

       310        320        330        340        350        360 
NYQPVKYTSL LADKVGCNVL DTVDMVDCLR QKSAKELVEQ DIQPARYHVA FGPVIDGDVI 

       370        380        390        400        410        420 
PDDPEILMEQ GEFLNYDIML GVNQGEGLKF VEGVVDPEDG VSGTDFDYSV SNFVDNLYGY 

       430        440        450        460        470        480 
PEGKDTLRET IKFMYTDWAD RDNPETRRKT LVALFTDHQW VEPSVVTADL HARYGSPTYF 

       490        500        510        520        530        540 
YAFYHHCQSL MKPAWSDAAH GDEVPYVFGV PMVGPTDLFP CNFSKNDVML SAVVMTYWTN 

       550        560        570        580        590        600 
FAKTGDPNKP VPQDTKFIHT KANRFEEVAW SKYNPRDQLY LHIGLKPRVR DHYRATKVAF 

       610        620        630        640        650        660 
WKHLVPHLYN LHDMFHYTST TTKVPPPDTT HSSHITRRPN GKTWSTKRPA ISPAYSNENA 

       670        680        690        700        710        720 
PGSWNGDQDA GPLLVENPRD YSTELSVTIA VGASLLFLNV LAFAALYYRK DKRRQEPLRQ 

       730        740        750        760        770        780 
PSPQRGTGAP ELGTAPEEEL AALQLGPTHH ECEAGPPHDT LRLTALPDYT LTLRRSPDDI 

       790        800        810        820 
PLMTPNTITM IPNSLVGLQT LHPYNTFAAG FNSTGLPHSH STTRV 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Hypothalamus and Retina.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Neuroligin 3 is a vertebrate gliotactin expressed in the olfactory ensheathing glia, a growth-promoting class of macroglia."
Gilbert M., Smith J., Roskams A.J., Auld V.J.
Glia 34:151-164(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]"Neuroligins determine synapse maturation and function."
Varoqueaux F., Aramuni G., Rawson R.L., Mohrmann R., Missler M., Gottmann K., Zhang W., Sudhof T.C., Brose N.
Neuron 51:741-754(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
[5]"Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses."
Budreck E.C., Scheiffele P.
Eur. J. Neurosci. 26:1738-1748(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NLGN1 AND NLGN2, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-769, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"Unusually rapid evolution of neuroligin-4 in mice."
Bolliger M.F., Pei J., Maxeiner S., Boucard A.A., Grishin N.V., Sudhof T.C.
Proc. Natl. Acad. Sci. U.S.A. 105:6421-6426(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Neuroligin-3-deficient mice: model of a monogenic heritable form of autism with an olfactory deficit."
Radyushkin K., Hammerschmidt K., Boretius S., Varoqueaux F., El-Kordi A., Ronnenberg A., Winter D., Frahm J., Fischer J., Brose N., Ehrenreich H.
Genes Brain Behav. 8:416-425(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[9]"The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions."
Bottos A., Destro E., Rissone A., Graziano S., Cordara G., Assenzio B., Cera M.R., Mascia L., Bussolino F., Arese M.
Proc. Natl. Acad. Sci. U.S.A. 106:20782-20787(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK039018 mRNA. Translation: BAC30207.1.
AK044438 mRNA. Translation: BAC31918.1.
AL683892 Genomic DNA. Translation: CAM24450.1.
RefSeqNP_766520.2. NM_172932.4.
UniGeneMm.121508.

3D structure databases

ProteinModelPortalQ8BYM5.
SMRQ8BYM5. Positions 39-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BYM5. 2 interactions.
MINTMINT-4103912.

Protein family/group databases

MEROPSS09.987.

PTM databases

PhosphoSiteQ8BYM5.

Proteomic databases

PaxDbQ8BYM5.
PRIDEQ8BYM5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065858; ENSMUSP00000066304; ENSMUSG00000031302.
GeneID245537.
KEGGmmu:245537.
UCSCuc009txj.3. mouse.

Organism-specific databases

CTD54413.
MGIMGI:2444609. Nlgn3.

Phylogenomic databases

eggNOGCOG2272.
GeneTreeENSGT00690000101920.
HOGENOMHOG000231424.
HOVERGENHBG008839.
KOK07378.
TreeFamTF326187.

Gene expression databases

BgeeQ8BYM5.
CleanExMM_NLGN3.
GenevestigatorQ8BYM5.

Family and domain databases

InterProIPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Neuroligin.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR01090. NEUROLIGIN.
PROSITEPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNLGN3. mouse.
NextBio386794.
PROQ8BYM5.
SOURCESearch...

Entry information

Entry nameNLGN3_MOUSE
AccessionPrimary (citable) accession number: Q8BYM5
Secondary accession number(s): A2AGI1, Q8BXR4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot