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Protein

TBC1 domain family member 4

Gene

Tbc1d4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 4
Alternative name(s):
Akt substrate of 160 kDa
Short name:
AS160
Gene namesi
Name:Tbc1d4
Synonyms:As160, Kiaa0603
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2429660. Tbc1d4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080271 – 1307TBC1 domain family member 4Add BLAST1307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei258PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1
Modified residuei320PhosphoserineBy similarity1
Modified residuei324Phosphoserine; by PKB/AKT11 Publication1
Modified residuei348Phosphoserine; by PKB/AKT11 Publication1
Modified residuei351PhosphoserineBy similarity1
Modified residuei484N6-acetyllysineBy similarity1
Modified residuei573PhosphoserineCombined sources1
Modified residuei575PhosphothreonineBy similarity1
Modified residuei577Phosphoserine; by PKB/AKT1Combined sources1 Publication1
Modified residuei584Omega-N-methylarginineCombined sources1
Modified residuei595Phosphoserine; by PKB/AKT12 Publications1
Modified residuei598PhosphoserineCombined sources1
Modified residuei616PhosphoserineCombined sources1
Modified residuei620PhosphothreonineCombined sources1
Modified residuei624PhosphoserineCombined sources1
Modified residuei649Phosphothreonine; by PKB/AKT12 Publications1
Modified residuei673PhosphoserineBy similarity1
Modified residuei758Phosphoserine; by PKB/AKT11 Publication1
Modified residuei761PhosphoserineCombined sources1
Modified residuei764PhosphoserineCombined sources1
Modified residuei770PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization.4 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BYJ6.
PeptideAtlasiQ8BYJ6.
PRIDEiQ8BYJ6.

PTM databases

iPTMnetiQ8BYJ6.
PhosphoSitePlusiQ8BYJ6.

Expressioni

Tissue specificityi

Widely expressed, including in pancreatic beta cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000033083.
CleanExiMM_TBC1D4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
YwhaeP622596EBI-7920707,EBI-356480

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229182. 1 interactor.
IntActiQ8BYJ6. 2 interactors.
MINTiMINT-4113377.
STRINGi10090.ENSMUSP00000125509.

Structurei

3D structure databases

ProteinModelPortaliQ8BYJ6.
SMRiQ8BYJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 209PID 1PROSITE-ProRule annotationAdd BLAST157
Domaini319 – 475PID 2PROSITE-ProRule annotationAdd BLAST157
Domaini927 – 1121Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST195

Sequence similaritiesi

Contains 2 PID domains.PROSITE-ProRule annotation
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4436. Eukaryota.
ENOG410YWJY. LUCA.
HOVERGENiHBG059376.
InParanoidiQ8BYJ6.
KOiK17902.
PhylomeDBiQ8BYJ6.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
InterProiIPR021785. DUF3350.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR000195. Rab-GTPase-TBC_dom.
IPR033564. TBC1D4.
[Graphical view]
PANTHERiPTHR22957:SF195. PTHR22957:SF195. 1 hit.
PfamiPF11830. DUF3350. 1 hit.
PF00640. PID. 2 hits.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 2 hits.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
SSF50729. SSF50729. 3 hits.
PROSITEiPS01179. PID. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BYJ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESPSCIQDE PFPHPLEPEP SAPAQPGATK PGDKRFRLWY VGGSCLDRRT
60 70 80 90 100
TLPMLPWLMA EIRRRSQKPD AGGCGAPAAR EVILVLSAPF LRCVPAPGAG
110 120 130 140 150
VGGGAGSGAV QPNTGVFIFE HKAQHISRFI HNSHDLTYFA YLIKAQPDDP
160 170 180 190 200
ESQMACHVFR ATDPNQVPDV ISSIRQLSKA AMKEDSKPSK DNEDAFYNSQ
210 220 230 240 250
KFEVLYCGRV IVTHKKAPSS LIDDCKDKFS LHEQQRLKLQ GERGGDPGDE
260 270 280 290 300
MGVLEVESPV SPDDSLPEKA DGTVNSPRAL PSLASLPALA SQPALASSRV
310 320 330 340 350
CFPERILEDC GFDEQQEFRS RCSSVTGVMQ KKVHENNQKT QPRRRHASAP
360 370 380 390 400
SHVQPSDSEK NRTMLFQVGR FEINLISPDT KSVVLEKNFK DISSCSQGIK
410 420 430 440 450
HVDHFGFICR ESPEPGLSQY ICYVFQCANE SLVDEVMLTL KQAFSTAAAL
460 470 480 490 500
QSAKTQIKLC ETCPMHSLHK LCERIEGLYP PRAKLVIQRH LSSLTDNEQA
510 520 530 540 550
DIFERVQKMK PISDQEENEL VILHLRQLCE AKQRTHVHIG EGPAIISNST
560 570 580 590 600
IPENVTSGGR FKLDVLKNKA KRSLTSSLEN IFSRGANRMR GRLGSMDSFE
610 620 630 640 650
RANSLASEKD FSPGDSPPGT PPASPLSSAW HAFPEEDSDS PQFRRRAHTF
660 670 680 690 700
SHPPSSSRRK LNLQDGKAHG LRSPLLRQSS SEQCSIVPSA RRMYKESNSS
710 720 730 740 750
CSLPSLHTSF SAPSFTAPSF LKSFYQNSGR LSPQYENEIR QDTASESSDG
760 770 780 790 800
EGRKRTSSTC SNESLNAGGT PVTPRRVSWR QRIFLRVASP VNKSPSAMQQ
810 820 830 840 850
QKDGLDRTEL LPLSPLSPTM EEEPLIIFLS GDEDTEKVEE KKKSKELKSL
860 870 880 890 900
WKKAIHQQIL LLRMEKENQK LEEARRDELQ SRKVKLDYEE VGTCQKEILI
910 920 930 940 950
AWDKKLLNCR TKIRCDMEDI HTSLKEGVPK SRRGEIWQFL ALQYRLRHRL
960 970 980 990 1000
PNKHQPPDTS YKELLKQLTA QQHAILVDLG RTFPTHPYFS VQLGAGQLSL
1010 1020 1030 1040 1050
FNLLKAYSLL DKEVGYCQGI SFVAGVLLLH MSEEQAFEML KFLMYDLGFR
1060 1070 1080 1090 1100
KQYRPDMMSL QIQMYQLSRL LHDYHRELYN HLEENEISPS LYAAPWFLTL
1110 1120 1130 1140 1150
FASQFPLGFV ARVFDIIFLQ GTEVIFKVAL SLLSSQEALI MECENFENIV
1160 1170 1180 1190 1200
EFLKSTLPDM NTTEMEKIIT QVFEMDISKQ LHAYEVEYHV LQDELLESSY
1210 1220 1230 1240 1250
ACEDNESLEK LERANNQLKR QNMDLLEKLQ VAHAKIQALE SNLETLLTRE
1260 1270 1280 1290 1300
TKMKALIRTL EQDKMAYQKT VEQIRKLLPA DALANCELLL KDLTHPTNDK

AKAGNKP
Length:1,307
Mass (Da):147,451
Last modified:June 7, 2005 - v2
Checksum:i60B37CC6ED1D2F55
GO
Isoform 2 (identifier: Q8BYJ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     685-747: Missing.
     873-873: E → G
     874-1307: Missing.

Show »
Length:810
Mass (Da):89,865
Checksum:iD9755CCAE0FD49EF
GO
Isoform 3 (identifier: Q8BYJ6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     685-739: Missing.
     873-873: E → G
     874-1307: Missing.

Note: No experimental confirmation available.
Show »
Length:818
Mass (Da):90,740
Checksum:i923910C328DA9D00
GO
Isoform 4 (identifier: Q8BYJ6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-508: Missing.
     685-747: Missing.
     873-873: E → G
     874-1307: Missing.

Note: No experimental confirmation available.
Show »
Length:809
Mass (Da):89,737
Checksum:i49D29FA0DEC1CA40
GO
Isoform 5 (identifier: Q8BYJ6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-793: Missing.
     794-802: SPSAMQQQK → MPLTVFFSA
     873-873: Missing.

Note: No experimental confirmation available.
Show »
Length:513
Mass (Da):59,706
Checksum:iAD9462305438B697
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171I → V in BAC30322 (PubMed:16141072).Curated1
Sequence conflicti631H → Y in BAC30322 (PubMed:16141072).Curated1
Sequence conflicti840E → A in BAC30322 (PubMed:16141072).Curated1
Sequence conflicti840E → K in AAH64433 (PubMed:15489334).Curated1
Sequence conflicti844S → K in AAH80762 (PubMed:15489334).Curated1
Sequence conflicti880Q → L in BAE42613 (PubMed:16141072).Curated1
Sequence conflicti914R → K in BAE42613 (PubMed:16141072).Curated1
Sequence conflicti974A → T in BAE42613 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0368721 – 793Missing in isoform 5. 1 PublicationAdd BLAST793
Alternative sequenceiVSP_036873508Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_013891685 – 747Missing in isoform 2 and isoform 4. 3 PublicationsAdd BLAST63
Alternative sequenceiVSP_036874685 – 739Missing in isoform 3. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_036875794 – 802SPSAMQQQK → MPLTVFFSA in isoform 5. 1 Publication9
Alternative sequenceiVSP_036876873E → G in isoform 2, isoform 3 and isoform 4. 3 Publications1
Alternative sequenceiVSP_036877873Missing in isoform 5. 1 Publication1
Alternative sequenceiVSP_036878874 – 1307Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsAdd BLAST434

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039337 mRNA. Translation: BAC30322.2.
AK044719 mRNA. Translation: BAC32048.1.
AK031120 mRNA. Translation: BAC27263.1.
AK171689 mRNA. Translation: BAE42613.1.
AK172575 mRNA. Translation: BAE43074.1.
BC064433 mRNA. Translation: AAH64433.1.
BC080762 mRNA. Translation: AAH80762.1.
BC117869 mRNA. Translation: AAI17870.1.
BC117870 mRNA. Translation: AAI17871.1.
AK220347 mRNA. Translation: BAD90243.1.
RefSeqiNP_001074747.2. NM_001081278.2.
XP_006518822.1. XM_006518759.3. [Q8BYJ6-1]
XP_011243304.1. XM_011245002.2. [Q8BYJ6-2]
UniGeneiMm.320639.

Genome annotation databases

GeneIDi210789.
KEGGimmu:210789.
UCSCiuc007uvl.1. mouse. [Q8BYJ6-5]
uc007uvo.2. mouse. [Q8BYJ6-3]
uc007uvp.2. mouse. [Q8BYJ6-4]
uc007uvq.2. mouse. [Q8BYJ6-2]
uc007uvr.2. mouse. [Q8BYJ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039337 mRNA. Translation: BAC30322.2.
AK044719 mRNA. Translation: BAC32048.1.
AK031120 mRNA. Translation: BAC27263.1.
AK171689 mRNA. Translation: BAE42613.1.
AK172575 mRNA. Translation: BAE43074.1.
BC064433 mRNA. Translation: AAH64433.1.
BC080762 mRNA. Translation: AAH80762.1.
BC117869 mRNA. Translation: AAI17870.1.
BC117870 mRNA. Translation: AAI17871.1.
AK220347 mRNA. Translation: BAD90243.1.
RefSeqiNP_001074747.2. NM_001081278.2.
XP_006518822.1. XM_006518759.3. [Q8BYJ6-1]
XP_011243304.1. XM_011245002.2. [Q8BYJ6-2]
UniGeneiMm.320639.

3D structure databases

ProteinModelPortaliQ8BYJ6.
SMRiQ8BYJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229182. 1 interactor.
IntActiQ8BYJ6. 2 interactors.
MINTiMINT-4113377.
STRINGi10090.ENSMUSP00000125509.

PTM databases

iPTMnetiQ8BYJ6.
PhosphoSitePlusiQ8BYJ6.

Proteomic databases

PaxDbiQ8BYJ6.
PeptideAtlasiQ8BYJ6.
PRIDEiQ8BYJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi210789.
KEGGimmu:210789.
UCSCiuc007uvl.1. mouse. [Q8BYJ6-5]
uc007uvo.2. mouse. [Q8BYJ6-3]
uc007uvp.2. mouse. [Q8BYJ6-4]
uc007uvq.2. mouse. [Q8BYJ6-2]
uc007uvr.2. mouse. [Q8BYJ6-1]

Organism-specific databases

CTDi9882.
MGIiMGI:2429660. Tbc1d4.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4436. Eukaryota.
ENOG410YWJY. LUCA.
HOVERGENiHBG059376.
InParanoidiQ8BYJ6.
KOiK17902.
PhylomeDBiQ8BYJ6.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

PROiQ8BYJ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033083.
CleanExiMM_TBC1D4.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
InterProiIPR021785. DUF3350.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR000195. Rab-GTPase-TBC_dom.
IPR033564. TBC1D4.
[Graphical view]
PANTHERiPTHR22957:SF195. PTHR22957:SF195. 1 hit.
PfamiPF11830. DUF3350. 1 hit.
PF00640. PID. 2 hits.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 2 hits.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
SSF50729. SSF50729. 3 hits.
PROSITEiPS01179. PID. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBCD4_MOUSE
AccessioniPrimary (citable) accession number: Q8BYJ6
Secondary accession number(s): Q149C0
, Q149C1, Q3T9E8, Q3TAQ5, Q5DU23, Q66JU2, Q6P2M2, Q8BMH6, Q8BXM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.