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Q8BYJ6 (TBCD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TBC1 domain family member 4
Alternative name(s):
Akt substrate of 160 kDa
Short name=AS160
Gene names
Name:Tbc1d4
Synonyms:As160, Kiaa0603
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake By similarity.

Subcellular location

Cytoplasm. Note: Cytoplasmic perinuclear By similarity. Ref.4

Tissue specificity

Widely expressed, including in pancreatic beta cells. Ref.4 Ref.9

Post-translational modification

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Ref.4 Ref.5 Ref.7 Ref.8

Sequence similarities

Contains 2 PID domains.

Contains 1 Rab-GAP TBC domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YwhaeP622596EBI-7920707,EBI-356480

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BYJ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BYJ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     685-747: Missing.
     873-873: E → G
     874-1307: Missing.
Isoform 3 (identifier: Q8BYJ6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     685-739: Missing.
     873-873: E → G
     874-1307: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8BYJ6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     508-508: Missing.
     685-747: Missing.
     873-873: E → G
     874-1307: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q8BYJ6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-793: Missing.
     794-802: SPSAMQQQK → MPLTVFFSA
     873-873: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13071307TBC1 domain family member 4
PRO_0000208027

Regions

Domain53 – 209157PID 1
Domain319 – 475157PID 2
Domain927 – 1121195Rab-GAP TBC

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue3241Phosphoserine; by PKB/AKT1 Ref.5
Modified residue3481Phosphoserine; by PKB/AKT1 Ref.5
Modified residue3511Phosphoserine By similarity
Modified residue4841N6-acetyllysine By similarity
Modified residue5731Phosphoserine By similarity
Modified residue5751Phosphothreonine By similarity
Modified residue5771Phosphoserine; by PKB/AKT1 Ref.5
Modified residue5951Phosphoserine; by PKB/AKT1 Ref.4 Ref.5
Modified residue5981Phosphoserine By similarity
Modified residue6491Phosphothreonine; by PKB/AKT1 Ref.4 Ref.5
Modified residue7581Phosphoserine; by PKB/AKT1 Ref.5

Natural variations

Alternative sequence1 – 793793Missing in isoform 5.
VSP_036872
Alternative sequence5081Missing in isoform 4.
VSP_036873
Alternative sequence685 – 74763Missing in isoform 2 and isoform 4.
VSP_013891
Alternative sequence685 – 73955Missing in isoform 3.
VSP_036874
Alternative sequence794 – 8029SPSAMQQQK → MPLTVFFSA in isoform 5.
VSP_036875
Alternative sequence8731E → G in isoform 2, isoform 3 and isoform 4.
VSP_036876
Alternative sequence8731Missing in isoform 5.
VSP_036877
Alternative sequence874 – 1307434Missing in isoform 2, isoform 3 and isoform 4.
VSP_036878

Experimental info

Sequence conflict1711I → V in BAC30322. Ref.1
Sequence conflict6311H → Y in BAC30322. Ref.1
Sequence conflict8401E → A in BAC30322. Ref.1
Sequence conflict8401E → K in AAH64433. Ref.2
Sequence conflict8441S → K in AAH80762. Ref.2
Sequence conflict8801Q → L in BAE42613. Ref.1
Sequence conflict9141R → K in BAE42613. Ref.1
Sequence conflict9741A → T in BAE42613. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 60B37CC6ED1D2F55

FASTA1,307147,451
        10         20         30         40         50         60 
MESPSCIQDE PFPHPLEPEP SAPAQPGATK PGDKRFRLWY VGGSCLDRRT TLPMLPWLMA 

        70         80         90        100        110        120 
EIRRRSQKPD AGGCGAPAAR EVILVLSAPF LRCVPAPGAG VGGGAGSGAV QPNTGVFIFE 

       130        140        150        160        170        180 
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPNQVPDV ISSIRQLSKA 

       190        200        210        220        230        240 
AMKEDSKPSK DNEDAFYNSQ KFEVLYCGRV IVTHKKAPSS LIDDCKDKFS LHEQQRLKLQ 

       250        260        270        280        290        300 
GERGGDPGDE MGVLEVESPV SPDDSLPEKA DGTVNSPRAL PSLASLPALA SQPALASSRV 

       310        320        330        340        350        360 
CFPERILEDC GFDEQQEFRS RCSSVTGVMQ KKVHENNQKT QPRRRHASAP SHVQPSDSEK 

       370        380        390        400        410        420 
NRTMLFQVGR FEINLISPDT KSVVLEKNFK DISSCSQGIK HVDHFGFICR ESPEPGLSQY 

       430        440        450        460        470        480 
ICYVFQCANE SLVDEVMLTL KQAFSTAAAL QSAKTQIKLC ETCPMHSLHK LCERIEGLYP 

       490        500        510        520        530        540 
PRAKLVIQRH LSSLTDNEQA DIFERVQKMK PISDQEENEL VILHLRQLCE AKQRTHVHIG 

       550        560        570        580        590        600 
EGPAIISNST IPENVTSGGR FKLDVLKNKA KRSLTSSLEN IFSRGANRMR GRLGSMDSFE 

       610        620        630        640        650        660 
RANSLASEKD FSPGDSPPGT PPASPLSSAW HAFPEEDSDS PQFRRRAHTF SHPPSSSRRK 

       670        680        690        700        710        720 
LNLQDGKAHG LRSPLLRQSS SEQCSIVPSA RRMYKESNSS CSLPSLHTSF SAPSFTAPSF 

       730        740        750        760        770        780 
LKSFYQNSGR LSPQYENEIR QDTASESSDG EGRKRTSSTC SNESLNAGGT PVTPRRVSWR 

       790        800        810        820        830        840 
QRIFLRVASP VNKSPSAMQQ QKDGLDRTEL LPLSPLSPTM EEEPLIIFLS GDEDTEKVEE 

       850        860        870        880        890        900 
KKKSKELKSL WKKAIHQQIL LLRMEKENQK LEEARRDELQ SRKVKLDYEE VGTCQKEILI 

       910        920        930        940        950        960 
AWDKKLLNCR TKIRCDMEDI HTSLKEGVPK SRRGEIWQFL ALQYRLRHRL PNKHQPPDTS 

       970        980        990       1000       1010       1020 
YKELLKQLTA QQHAILVDLG RTFPTHPYFS VQLGAGQLSL FNLLKAYSLL DKEVGYCQGI 

      1030       1040       1050       1060       1070       1080 
SFVAGVLLLH MSEEQAFEML KFLMYDLGFR KQYRPDMMSL QIQMYQLSRL LHDYHRELYN 

      1090       1100       1110       1120       1130       1140 
HLEENEISPS LYAAPWFLTL FASQFPLGFV ARVFDIIFLQ GTEVIFKVAL SLLSSQEALI 

      1150       1160       1170       1180       1190       1200 
MECENFENIV EFLKSTLPDM NTTEMEKIIT QVFEMDISKQ LHAYEVEYHV LQDELLESSY 

      1210       1220       1230       1240       1250       1260 
ACEDNESLEK LERANNQLKR QNMDLLEKLQ VAHAKIQALE SNLETLLTRE TKMKALIRTL 

      1270       1280       1290       1300 
EQDKMAYQKT VEQIRKLLPA DALANCELLL KDLTHPTNDK AKAGNKP 

« Hide

Isoform 2 [UniParc].

Checksum: D9755CCAE0FD49EF
Show »

FASTA81089,865
Isoform 3 [UniParc].

Checksum: 923910C328DA9D00
Show »

FASTA81890,740
Isoform 4 [UniParc].

Checksum: 49D29FA0DEC1CA40
Show »

FASTA80989,737
Isoform 5 [UniParc].

Checksum: AD9462305438B697
Show »

FASTA51359,706

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-840 (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Forelimb, Retina, Spinal cord and Spleen.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-844 (ISOFORM 2).
Strain: NMRI.
Tissue: Mammary tumor.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 289-1307 (ISOFORM 2).
Tissue: Fetal brain.
[4]"A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain."
Kane S., Sano H., Liu S.C.H., Asara J.M., Lane W.S., Garner C.C., Lienhard G.E.
J. Biol. Chem. 277:22115-22118(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-595 AND THR-649, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation."
Sano H., Kane S., Sano E., Miinea C.P., Asara J.M., Lane W.S., Garner C.W., Lienhard G.E.
J. Biol. Chem. 278:14599-14602(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-324; SER-348; SER-577; SER-595; THR-649 AND SER-758.
[6]"Insulin stimulation of GLUT4 exocytosis, but not its inhibition of endocytosis, is dependent on RabGAP AS160."
Zeigerer A., McBrayer M.K., McGraw T.E.
Mol. Biol. Cell 15:4406-4415(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: EFFECT ON GLUT4 TRANSLOCATION.
[7]"AMPK-mediated AS160 phosphorylation in skeletal muscle is dependent on AMPK catalytic and regulatory subunits."
Treebak J.T., Glund S., Deshmukh A., Klein D.K., Long Y.C., Jensen T.E., Jorgensen S.B., Viollet B., Andersson L., Neumann D., Wallimann T., Richter E.A., Chibalin A.V., Zierath J.R., Wojtaszewski J.F.
Diabetes 55:2051-2058(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY AMPK.
[8]"Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle."
Kramer H.F., Witczak C.A., Fujii N., Jessen N., Taylor E.B., Arnolds D.E., Sakamoto K., Hirshman M.F., Goodyear L.J.
Diabetes 55:2067-2076(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY AMPK.
[9]"Rab GTPase-activating protein AS160 is a major downstream effector of protein kinase B/Akt signaling in pancreatic beta-cells."
Bouzakri K., Ribaux P., Tomas A., Parnaud G., Rickenbach K., Halban P.A.
Diabetes 57:1195-1204(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK039337 mRNA. Translation: BAC30322.2.
AK044719 mRNA. Translation: BAC32048.1.
AK031120 mRNA. Translation: BAC27263.1.
AK171689 mRNA. Translation: BAE42613.1.
AK172575 mRNA. Translation: BAE43074.1.
BC064433 mRNA. Translation: AAH64433.1.
BC080762 mRNA. Translation: AAH80762.1.
BC117869 mRNA. Translation: AAI17870.1.
BC117870 mRNA. Translation: AAI17871.1.
AK220347 mRNA. Translation: BAD90243.1.
RefSeqNP_001074747.2. NM_001081278.2.
XP_006518822.1. XM_006518759.1. [Q8BYJ6-1]
UniGeneMm.320639.

3D structure databases

ProteinModelPortalQ8BYJ6.
SMRQ8BYJ6. Positions 883-1177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229182. 1 interaction.
IntActQ8BYJ6. 2 interactions.
MINTMINT-4113377.

PTM databases

PhosphoSiteQ8BYJ6.

Proteomic databases

MaxQBQ8BYJ6.
PaxDbQ8BYJ6.
PRIDEQ8BYJ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID210789.
KEGGmmu:210789.
UCSCuc007uvl.1. mouse. [Q8BYJ6-5]
uc007uvo.2. mouse. [Q8BYJ6-3]
uc007uvp.2. mouse. [Q8BYJ6-4]
uc007uvq.2. mouse. [Q8BYJ6-2]
uc007uvr.2. mouse. [Q8BYJ6-1]

Organism-specific databases

CTD9882.
MGIMGI:2429660. Tbc1d4.
RougeSearch...

Phylogenomic databases

eggNOGCOG5210.
HOVERGENHBG059376.
InParanoidQ3T9E8.
KOK17902.
PhylomeDBQ8BYJ6.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.
REACT_209837. Membrane Trafficking.

Gene expression databases

ArrayExpressQ8BYJ6.
BgeeQ8BYJ6.
CleanExMM_TBC1D4.
GenevestigatorQ8BYJ6.

Family and domain databases

Gene3D2.30.29.30. 3 hits.
InterProIPR021785. DUF3350.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamPF11830. DUF3350. 1 hit.
PF00640. PID. 2 hits.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTSM00462. PTB. 2 hits.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMSSF47923. SSF47923. 2 hits.
PROSITEPS01179. PID. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio373057.
PROQ8BYJ6.
SOURCESearch...

Entry information

Entry nameTBCD4_MOUSE
AccessionPrimary (citable) accession number: Q8BYJ6
Secondary accession number(s): Q149C0 expand/collapse secondary AC list , Q149C1, Q3T9E8, Q3TAQ5, Q5DU23, Q66JU2, Q6P2M2, Q8BMH6, Q8BXM2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot