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Protein

Sodium-coupled monocarboxylate transporter 1

Gene

Slc5a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na+:substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na+ gradient and hence contribute to the maintenance of the energy status and function of neurons.4 Publications

Kineticsi

  1. KM=296 µM for nicotinate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Apoptosis, Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiR-MMU-428643. Organic anion transporters.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled monocarboxylate transporter 1
    Alternative name(s):
    Electrogenic sodium monocarboxylate cotransporter
    Solute carrier family 5 member 8
    Gene namesi
    Name:Slc5a8Imported
    Synonyms:Smct1 Publication, Smct1By similarity
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 10

    Organism-specific databases

    MGIiMGI:2384916. Slc5a8.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 9ExtracellularSequence analysis9
    Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
    Topological domaini31 – 51CytoplasmicSequence analysisAdd BLAST21
    Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
    Topological domaini73 – 86ExtracellularSequence analysisAdd BLAST14
    Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
    Topological domaini108 – 132CytoplasmicSequence analysisAdd BLAST25
    Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
    Topological domaini154 – 161ExtracellularSequence analysis8
    Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
    Topological domaini183 – 184CytoplasmicSequence analysis2
    Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
    Topological domaini206 – 239ExtracellularSequence analysisAdd BLAST34
    Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
    Topological domaini261 – 279CytoplasmicSequence analysisAdd BLAST19
    Transmembranei280 – 300HelicalSequence analysisAdd BLAST21
    Topological domaini301 – 336ExtracellularSequence analysisAdd BLAST36
    Transmembranei337 – 359HelicalSequence analysisAdd BLAST23
    Topological domaini360 – 389CytoplasmicSequence analysisAdd BLAST30
    Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
    Topological domaini411 – 415ExtracellularSequence analysis5
    Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
    Topological domaini437 – 439CytoplasmicSequence analysis3
    Transmembranei440 – 460HelicalSequence analysisAdd BLAST21
    Topological domaini461 – 518ExtracellularSequence analysisAdd BLAST58
    Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
    Topological domaini540 – 611CytoplasmicSequence analysisAdd BLAST72

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003345001 – 611Sodium-coupled monocarboxylate transporter 1Add BLAST611

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi485N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BYF6.
    PaxDbiQ8BYF6.
    PRIDEiQ8BYF6.

    PTM databases

    iPTMnetiQ8BYF6.
    PhosphoSitePlusiQ8BYF6.

    Expressioni

    Tissue specificityi

    Expressed in brain, colon, kidney and in the ileum and jejunum of small intestine. In the kidney, expression occurred in the proximal tubule and the loop of Henle, being restricted to tubular epithelial cells in both the cortex and the medulla. In the colon, predominantly expressed in the distal half of the large bowel and in the most terminal ileum. Localized selectively in the luminal surface of crypts in the large intestine and to the brush border in the middle parts of crypts in the cecum. In the brain, expression was seen throughout, exclusively in neurons, including the cortex, hippocampus, cerebellum and pituitary gland (at protein level). Expression is reduced in oligodendrogliomas.5 Publications

    Gene expression databases

    BgeeiENSMUSG00000020062.
    GenevisibleiQ8BYF6. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000020255.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BYF6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2349. Eukaryota.
    COG0591. LUCA.
    GeneTreeiENSGT00760000118955.
    HOGENOMiHOG000261662.
    HOVERGENiHBG057280.
    InParanoidiQ8BYF6.
    KOiK14388.
    OMAiDILQDFP.
    OrthoDBiEOG091G07OC.
    PhylomeDBiQ8BYF6.
    TreeFamiTF316728.

    Family and domain databases

    InterProiIPR001734. Na/solute_symporter.
    [Graphical view]
    PANTHERiPTHR11819. PTHR11819. 2 hits.
    PfamiPF00474. SSF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00813. sss. 1 hit.
    PROSITEiPS50283. NA_SOLUT_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BYF6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDASRDIGSF VVWDYVVFAG MLLISAAIGI YYAFAGGGQQ TSKDFLMGGR
    60 70 80 90 100
    SMSAVPVALS LTASFMSAVT VLGTPAEVYR FGAIFSIFVI TYFFVVVISA
    110 120 130 140 150
    EVFLPVFYRL GITSTYEYLE LRFNRCIRLC GTILFIVQTI LYTGIVIYAP
    160 170 180 190 200
    ALALNQVTGF DLWGAVVATG VVCTFYCTLG GLKAVVWTDV FQVGIMVAGF
    210 220 230 240 250
    ASVIIQASIT QHGINKILSD AFNGGRLNFW NFDPNPLQRH TFWTIVIGGT
    260 270 280 290 300
    FTWTTIYGVN QSQVQRYISC KSRLHAKLSL YVNLVGLWVI LTCSIFCGLA
    310 320 330 340 350
    LYSRYRECDP WTSKKVSAID QLMPYLVLDI LKNYPGVPGL FVACAYSGTL
    360 370 380 390 400
    STVSSSINAL AAVTVEDLIK PRFKSLSEKS LSWISQGMSV LYGALCIGMA
    410 420 430 440 450
    ALASLMGALL QAALSIFGMV GGPLLGLFSL GILVPFANSI GALTGLLAGF
    460 470 480 490 500
    AISLWVGIGA QLYPPLPERT LPLPLETYGC NITHNGSDWM STTEMPFSTS
    510 520 530 540 550
    AFQIHNAERT PLMDNWYSLS YLYFSTIGTL TTLFVGILIS LSTGGRKQNL
    560 570 580 590 600
    DPRFLLTKQD FLSNFDVFKK RNHVLNYKLH PVEVGGTDNP AFNHVELNFT
    610
    DHSGKINGTR L
    Length:611
    Mass (Da):66,766
    Last modified:March 1, 2003 - v1
    Checksum:i406A49B543EA1D96
    GO

    Sequence cautioni

    The sequence AAH17691 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti587T → N in AAH17691 (PubMed:15489334).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY484428 mRNA. Translation: AAS49159.1.
    AK039927 mRNA. Translation: BAC30480.1.
    BC017691 mRNA. Translation: AAH17691.1. Different initiation.
    CCDSiCCDS36026.1.
    RefSeqiNP_663398.2. NM_145423.2.
    UniGeneiMm.77381.

    Genome annotation databases

    EnsembliENSMUST00000020255; ENSMUSP00000020255; ENSMUSG00000020062.
    GeneIDi216225.
    KEGGimmu:216225.
    UCSCiuc007grz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY484428 mRNA. Translation: AAS49159.1.
    AK039927 mRNA. Translation: BAC30480.1.
    BC017691 mRNA. Translation: AAH17691.1. Different initiation.
    CCDSiCCDS36026.1.
    RefSeqiNP_663398.2. NM_145423.2.
    UniGeneiMm.77381.

    3D structure databases

    ProteinModelPortaliQ8BYF6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000020255.

    PTM databases

    iPTMnetiQ8BYF6.
    PhosphoSitePlusiQ8BYF6.

    Proteomic databases

    MaxQBiQ8BYF6.
    PaxDbiQ8BYF6.
    PRIDEiQ8BYF6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000020255; ENSMUSP00000020255; ENSMUSG00000020062.
    GeneIDi216225.
    KEGGimmu:216225.
    UCSCiuc007grz.1. mouse.

    Organism-specific databases

    CTDi160728.
    MGIiMGI:2384916. Slc5a8.

    Phylogenomic databases

    eggNOGiKOG2349. Eukaryota.
    COG0591. LUCA.
    GeneTreeiENSGT00760000118955.
    HOGENOMiHOG000261662.
    HOVERGENiHBG057280.
    InParanoidiQ8BYF6.
    KOiK14388.
    OMAiDILQDFP.
    OrthoDBiEOG091G07OC.
    PhylomeDBiQ8BYF6.
    TreeFamiTF316728.

    Enzyme and pathway databases

    ReactomeiR-MMU-428643. Organic anion transporters.

    Miscellaneous databases

    PROiQ8BYF6.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000020062.
    GenevisibleiQ8BYF6. MM.

    Family and domain databases

    InterProiIPR001734. Na/solute_symporter.
    [Graphical view]
    PANTHERiPTHR11819. PTHR11819. 2 hits.
    PfamiPF00474. SSF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00813. sss. 1 hit.
    PROSITEiPS50283. NA_SOLUT_SYMP_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSC5A8_MOUSE
    AccessioniPrimary (citable) accession number: Q8BYF6
    Secondary accession number(s): Q8VD01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2003
    Last modified: November 2, 2016
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Transport of D-lactate and pyruvate stimulated by alpha-cyano-4-hydroxycinnamic acid, but inhibited by the short-chain fatty acids acetate, propionate and butyrate.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.