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Q8BYB9

- PGLT1_MOUSE

UniProt

Q8BYB9 - PGLT1_MOUSE

Protein

Protein O-glucosyltransferase 1

Gene

Poglut1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Dual specificity glycosyltransferase. Catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to EGF repeats, such as those found in F7, F9 and NOTCH2, on the consensus sequence C-X-S-X-P-C. Positively regulates Notch signaling without affecting Notch ligand binding.1 Publication

    Catalytic activityi

    Transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxy group of an acceptor protein substrate.

    Pathwayi

    GO - Molecular functioni

    1. glucosyltransferase activity Source: MGI
    2. protein xylosyltransferase activity Source: UniProtKB-EC
    3. UDP-glucosyltransferase activity Source: UniProtKB
    4. UDP-xylosyltransferase activity Source: MGI

    GO - Biological processi

    1. cardiovascular system development Source: MGI
    2. protein O-linked glycosylation Source: MGI
    3. regulation of Notch signaling pathway Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_196492. Pre-NOTCH Processing in the Endoplasmic Reticulum.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT90. Glycosyltransferase Family 90.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-glucosyltransferase 1 (EC:2.4.1.-)
    Alternative name(s):
    CAP10-like 46 kDa protein
    KTEL motif-containing protein 1
    O-glucosyltransferase Rumi homolog
    Short name:
    Rumi
    Protein O-xylosyltransferase (EC:2.4.2.26)
    Gene namesi
    Name:Poglut1
    Synonyms:Clp46, Ktelc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:2444232. Poglut1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Mutant embryos die at or before 9.5 dpc. At 7.0 to 7.5 dpc, they cannot be morphologically distinguished from wild-type littermates. At 8.0 dpc, mutant embryos exhibit an abnormally expanded neural plate that does not fold properly, absence of heart rudiments and posterior axis truncation.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi389 – 3924Missing: Significantly more secreted than wild-type. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 392369Protein O-glucosyltransferase 1PRO_0000246686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BYB9.
    PaxDbiQ8BYB9.
    PRIDEiQ8BYB9.

    PTM databases

    PhosphoSiteiQ8BYB9.

    Expressioni

    Tissue specificityi

    Widely expressed in newborn and adult tissues (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ8BYB9.
    BgeeiQ8BYB9.
    CleanExiMM_KTELC1.
    GenevestigatoriQ8BYB9.

    Interactioni

    Protein-protein interaction databases

    BioGridi230254. 1 interaction.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi389 – 3924Endoplasmic reticulum retention signal

    Sequence similaritiesi

    Belongs to the glycosyltransferase 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248922.
    GeneTreeiENSGT00530000063132.
    HOVERGENiHBG069044.
    InParanoidiQ8BYB9.
    KOiK13667.
    OMAiINHLQMD.
    OrthoDBiEOG7R831J.
    PhylomeDBiQ8BYB9.
    TreeFamiTF323280.

    Family and domain databases

    InterProiIPR006598. LipoPS_modifying.
    [Graphical view]
    PfamiPF05686. Glyco_transf_90. 1 hit.
    [Graphical view]
    SMARTiSM00672. CAP10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BYB9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERRAGSRLR AWMLLLLLCP VQGRQKDSGS KWKVFLDQIN RALENYEPCS    50
    SQNCSCYHGV IEEDLTPFRG GISRKMMAEV VRRKLGTHYQ IIKNRLFRED 100
    DCMFPSRCSG VEHFILEVIH RLPDMEMVIN VRDYPQVPKW MEPTIPVFSF 150
    SKTSEYHDIM YPAWTFWEGG PAVWPLYPTG LGRWDLFRED LLRSAAQWPW 200
    EKKNSTAYFR GSRTSPERDP LILLSRKNPK LVDAEYTKNQ AWKSMKDTLG 250
    KPAAKDVHLI DHCKYRYLFN FRGVAASFRF KHLFLCGSLV FHVGDEWVEF 300
    FYPQLKPWVH YIPVKTDLSN VQELLQFVKA NDDIAQEIAK RGSQFIINHL 350
    QMDDITCYWE NLLTDYSKFL SYNVTRRKDY YQIVPRRLKT EL 392
    Length:392
    Mass (Da):46,379
    Last modified:July 25, 2006 - v2
    Checksum:iCAAD9133E47D3EF0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531N → S in BAC30905. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK031608 mRNA. Translation: BAC27475.1.
    AK035948 mRNA. Translation: BAC29255.1.
    AK036224 mRNA. Translation: BAC29351.1.
    AK041321 mRNA. Translation: BAC30905.1.
    BC026809 mRNA. Translation: AAH26809.1.
    CCDSiCCDS28170.1.
    RefSeqiNP_759012.1. NM_172380.3.
    UniGeneiMm.284366.
    Mm.475345.

    Genome annotation databases

    EnsembliENSMUST00000036210; ENSMUSP00000038166; ENSMUSG00000034064.
    GeneIDi224143.
    KEGGimmu:224143.
    UCSCiuc007zfc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK031608 mRNA. Translation: BAC27475.1 .
    AK035948 mRNA. Translation: BAC29255.1 .
    AK036224 mRNA. Translation: BAC29351.1 .
    AK041321 mRNA. Translation: BAC30905.1 .
    BC026809 mRNA. Translation: AAH26809.1 .
    CCDSi CCDS28170.1.
    RefSeqi NP_759012.1. NM_172380.3.
    UniGenei Mm.284366.
    Mm.475345.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230254. 1 interaction.

    Protein family/group databases

    CAZyi GT90. Glycosyltransferase Family 90.

    PTM databases

    PhosphoSitei Q8BYB9.

    Proteomic databases

    MaxQBi Q8BYB9.
    PaxDbi Q8BYB9.
    PRIDEi Q8BYB9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036210 ; ENSMUSP00000038166 ; ENSMUSG00000034064 .
    GeneIDi 224143.
    KEGGi mmu:224143.
    UCSCi uc007zfc.1. mouse.

    Organism-specific databases

    CTDi 56983.
    MGIi MGI:2444232. Poglut1.

    Phylogenomic databases

    eggNOGi NOG248922.
    GeneTreei ENSGT00530000063132.
    HOVERGENi HBG069044.
    InParanoidi Q8BYB9.
    KOi K13667.
    OMAi INHLQMD.
    OrthoDBi EOG7R831J.
    PhylomeDBi Q8BYB9.
    TreeFami TF323280.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196492. Pre-NOTCH Processing in the Endoplasmic Reticulum.

    Miscellaneous databases

    ChiTaRSi POGLUT1. mouse.
    NextBioi 377117.
    PROi Q8BYB9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BYB9.
    Bgeei Q8BYB9.
    CleanExi MM_KTELC1.
    Genevestigatori Q8BYB9.

    Family and domain databases

    InterProi IPR006598. LipoPS_modifying.
    [Graphical view ]
    Pfami PF05686. Glyco_transf_90. 1 hit.
    [Graphical view ]
    SMARTi SM00672. CAP10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum, Testis and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    3. "Regulation of mammalian Notch signaling and embryonic development by the protein O-glucosyltransferase Rumi."
      Fernandez-Valdivia R., Takeuchi H., Samarghandi A., Lopez M., Leonardi J., Haltiwanger R.S., Jafar-Nejad H.
      Development 138:1925-1934(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    4. Cited for: FUNCTION, MUTAGENESIS OF 389-LYS--LEU-392.

    Entry informationi

    Entry nameiPGLT1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BYB9
    Secondary accession number(s): Q8R0H7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3