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Q8BY89 (CTL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline transporter-like protein 2
Alternative name(s):
Solute carrier family 44 member 2
Gene names
Name:Slc44a2
Synonyms:Ctl2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Choline transporter By similarity.

Subunit structure

Interacts with COCH By similarity.

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed at high levels in lung, colon, inner ear and spleen (at protein level). Progressively lower levels in brain, tongue, liver and kidney (at protein level). In the kidney, prominent expression in glomeruli in the lining of Bowman's capsule and on the mesangial cells adjacent to the vessels within the glomerulus (at protein level). Strongly expressed on the membranes of splenocytes and in lung parenchyme (at protein level). Isoform 1 is expressed at higher levels than isoform 2 in colon, heart, kidney, lung, cochlea, tongue and muscle, as well as in the inner ear. Isoform 2 is predominant in brain, liver and spleen. Ref.5

Sequence similarities

Belongs to the CTL (choline transporter-like) family.

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform 1 (identifier: Q8BY89-1)

Also known as: CTL2a; CTL2P2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BY89-2)

Also known as: CTL2b; CTL2P1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGKDSQNYYGKH → MEDDRKDAVY
Note: Produced by alternative promoter usage.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Choline transporter-like protein 2
PRO_0000191718

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Potential
Topological domain55 – 232178Extracellular Potential
Transmembrane233 – 25321Helical; Potential
Topological domain254 – 2563Cytoplasmic Potential
Transmembrane257 – 27721Helical; Potential
Topological domain278 – 31538Extracellular Potential
Transmembrane316 – 33621Helical; Potential
Topological domain337 – 36428Cytoplasmic Potential
Transmembrane365 – 38521Helical; Potential
Topological domain386 – 45469Extracellular Potential
Transmembrane455 – 47723Helical; Potential
Topological domain478 – 50427Cytoplasmic Potential
Transmembrane505 – 52521Helical; Potential
Topological domain526 – 56338Extracellular Potential
Transmembrane564 – 58421Helical; Potential
Topological domain585 – 59915Cytoplasmic Potential
Transmembrane600 – 62021Helical; Potential
Topological domain621 – 63818Extracellular Potential
Transmembrane639 – 65921Helical; Potential
Topological domain660 – 70647Cytoplasmic Potential

Amino acid modifications

Glycosylation1871N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation2001N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation4171N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1212MGKDS…YYGKH → MEDDRKDAVY in isoform 2.
VSP_015432

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CTL2a) (CTL2P2) [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 954AFD393C69A009

FASTA70680,110
        10         20         30         40         50         60 
MGKDSQNYYG KHGTPQKYDP TFKGPIYNRG CTDVICCVLL FLAIVGYVAV GIIAWTHGDP 

        70         80         90        100        110        120 
RKVIYPTDSR GEFCGQKGTK NADKPFLFYF NIVKCANPLV LLEFHCPTPQ ICVKQCPDRY 

       130        140        150        160        170        180 
LTLLSARNTR DFDYYKQFCV PGFQNNKGVT EILRDGECPA VITPSKPLAQ RCFPAIHASK 

       190        200        210        220        230        240 
GVLMVGNETT YEDGHGARKN ITDLVEGAKK ANKILEARQL AMQIFEDYTV SWYWIIIGLV 

       250        260        270        280        290        300 
IAMVLSLLFI VLLRFLAGIM VWVMIVMVIL VLGYGIFHCY MEYSRLRGEA GSDVSLVDLG 

       310        320        330        340        350        360 
FQTDLRVYLH LRQTWMAFMI ILSILEVVII LLLIFLRKRI LIAIALIKEA SRAVGHVMCS 

       370        380        390        400        410        420 
LLYPLVTFFL LCLCIAYWAS TSVFLSTSNT AVYKVVDDTA CPLLRKTCNP ETFPLRNESL 

       430        440        450        460        470        480 
QCPTARCQFA FYGGESTYHR ALLGLQIFNA FMFFWLANFV LALGQVTLAG AFASYYWAMR 

       490        500        510        520        530        540 
KPDDMPAFPL FSAFGRALRY HTGSLAFGSL ILAIVQIIRV MLEYLDQRLK AAQNKFAKFL 

       550        560        570        580        590        600 
MVCLKCCFWC LEKFIKFLNR NAYIMIAIYG TNFCTSARNA FFLLMRNIIR VAVLDKVTDF 

       610        620        630        640        650        660 
LFLLGKLLIV GSVGILAFFF FTHRIRIVQD TAPPLNYYWV PILTVIIGSY LIAHGFFSVY 

       670        680        690        700 
GMCVDTLFLC FLEDLERNDG SAERPYFMSS TLKKLLNKTN KKVAES 

« Hide

Isoform 2 (CTL2b) (CTL2P1) [UniParc].

Checksum: 17E13C468805E580
Show »

FASTA70479,924

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: Czech II.
Tissue: Mammary gland.
[3]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-187 AND ASN-200.
Tissue: Myoblast.
[4]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-187 AND ASN-200.
[5]"Isoforms, expression, glycosylation, and tissue distribution of CTL2/SLC44A2."
Kommareddi P.K., Nair T.S., Thang L.V., Galano M.M., Babu E., Ganapathy V., Kanazawa T., McHugh J.B., Carey T.E.
Protein J. 29:417-426(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ISOFORMS 1 AND 3, ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY, GLYCOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK041533 mRNA. Translation: BAC30976.1.
AK048648 mRNA. Translation: BAC33409.1.
BC031535 mRNA. Translation: AAH31535.1.
CCDSCCDS22903.1. [Q8BY89-1]
RefSeqNP_001186115.1. NM_001199186.1. [Q8BY89-2]
NP_690021.1. NM_152808.3. [Q8BY89-1]
UniGeneMm.148425.

3D structure databases

ProteinModelPortalQ8BY89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BY89. 1 interaction.
MINTMINT-4997673.
STRING10090.ENSMUSP00000034697.

Protein family/group databases

TCDB2.A.92.1.2. the choline transporter-like (ctl) family.

PTM databases

PhosphoSiteQ8BY89.

Proteomic databases

MaxQBQ8BY89.
PaxDbQ8BY89.
PRIDEQ8BY89.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034697; ENSMUSP00000034697; ENSMUSG00000057193. [Q8BY89-1]
GeneID68682.
KEGGmmu:68682.
UCSCuc009okz.2. mouse. [Q8BY89-2]
uc009ola.2. mouse. [Q8BY89-1]

Organism-specific databases

CTD57153.
MGIMGI:1915932. Slc44a2.

Phylogenomic databases

eggNOGNOG310633.
GeneTreeENSGT00550000074521.
HOGENOMHOG000007110.
HOVERGENHBG062155.
KOK15377.
OMAQTWMAFM.
OrthoDBEOG7FR7G4.
TreeFamTF313325.

Gene expression databases

BgeeQ8BY89.
CleanExMM_SLC44A2.
GenevestigatorQ8BY89.

Family and domain databases

InterProIPR007603. Choline_transptr-like.
[Graphical view]
PANTHERPTHR12385. PTHR12385. 1 hit.
PfamPF04515. Choline_transpo. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC44A2. mouse.
NextBio327690.
PROQ8BY89.
SOURCESearch...

Entry information

Entry nameCTL2_MOUSE
AccessionPrimary (citable) accession number: Q8BY89
Secondary accession number(s): Q8K2F1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot