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Protein

Choline transporter-like protein 2

Gene

Slc44a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Choline transporter.By similarity

GO - Molecular functioni

  1. signal transducer activity Source: MGI

GO - Biological processi

  1. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  2. signal transduction Source: MGI
  3. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_271919. Synthesis of PC.
REACT_346270. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi2.A.92.1.2. the choline transporter-like (ctl) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline transporter-like protein 2
Alternative name(s):
Solute carrier family 44 member 2
Gene namesi
Name:Slc44a2
Synonyms:Ctl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1915932. Slc44a2.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence AnalysisAdd
BLAST
Topological domaini55 – 232178ExtracellularSequence AnalysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence AnalysisAdd
BLAST
Topological domaini254 – 2563CytoplasmicSequence Analysis
Transmembranei257 – 27721HelicalSequence AnalysisAdd
BLAST
Topological domaini278 – 31538ExtracellularSequence AnalysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence AnalysisAdd
BLAST
Topological domaini337 – 36428CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei365 – 38521HelicalSequence AnalysisAdd
BLAST
Topological domaini386 – 45469ExtracellularSequence AnalysisAdd
BLAST
Transmembranei455 – 47723HelicalSequence AnalysisAdd
BLAST
Topological domaini478 – 50427CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei505 – 52521HelicalSequence AnalysisAdd
BLAST
Topological domaini526 – 56338ExtracellularSequence AnalysisAdd
BLAST
Transmembranei564 – 58421HelicalSequence AnalysisAdd
BLAST
Topological domaini585 – 59915CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence AnalysisAdd
BLAST
Topological domaini621 – 63818ExtracellularSequence AnalysisAdd
BLAST
Transmembranei639 – 65921HelicalSequence AnalysisAdd
BLAST
Topological domaini660 – 70647CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Choline transporter-like protein 2PRO_0000191718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi187 – 1871N-linked (GlcNAc...)2 Publications
Glycosylationi200 – 2001N-linked (GlcNAc...)2 Publications
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BY89.
PaxDbiQ8BY89.
PRIDEiQ8BY89.

PTM databases

PhosphoSiteiQ8BY89.

Expressioni

Tissue specificityi

Expressed at high levels in lung, colon, inner ear and spleen (at protein level). Progressively lower levels in brain, tongue, liver and kidney (at protein level). In the kidney, prominent expression in glomeruli in the lining of Bowman's capsule and on the mesangial cells adjacent to the vessels within the glomerulus (at protein level). Strongly expressed on the membranes of splenocytes and in lung parenchyme (at protein level). Isoform 1 is expressed at higher levels than isoform 2 in colon, heart, kidney, lung, cochlea, tongue and muscle, as well as in the inner ear. Isoform 2 is predominant in brain, liver and spleen.1 Publication

Gene expression databases

BgeeiQ8BY89.
CleanExiMM_SLC44A2.
GenevestigatoriQ8BY89.

Interactioni

Subunit structurei

Interacts with COCH.By similarity

Protein-protein interaction databases

IntActiQ8BY89. 1 interaction.
MINTiMINT-4997673.
STRINGi10090.ENSMUSP00000034697.

Structurei

3D structure databases

ProteinModelPortaliQ8BY89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG310633.
GeneTreeiENSGT00550000074521.
HOGENOMiHOG000007110.
HOVERGENiHBG062155.
InParanoidiQ8BY89.
KOiK15377.
OMAiENRFAKF.
OrthoDBiEOG7FR7G4.
TreeFamiTF313325.

Family and domain databases

InterProiIPR007603. Choline_transptr-like.
[Graphical view]
PANTHERiPTHR12385. PTHR12385. 1 hit.
PfamiPF04515. Choline_transpo. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: Q8BY89-1) [UniParc]FASTAAdd to basket

Also known as: CTL2a, CTL2P2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKDSQNYYG KHGTPQKYDP TFKGPIYNRG CTDVICCVLL FLAIVGYVAV
60 70 80 90 100
GIIAWTHGDP RKVIYPTDSR GEFCGQKGTK NADKPFLFYF NIVKCANPLV
110 120 130 140 150
LLEFHCPTPQ ICVKQCPDRY LTLLSARNTR DFDYYKQFCV PGFQNNKGVT
160 170 180 190 200
EILRDGECPA VITPSKPLAQ RCFPAIHASK GVLMVGNETT YEDGHGARKN
210 220 230 240 250
ITDLVEGAKK ANKILEARQL AMQIFEDYTV SWYWIIIGLV IAMVLSLLFI
260 270 280 290 300
VLLRFLAGIM VWVMIVMVIL VLGYGIFHCY MEYSRLRGEA GSDVSLVDLG
310 320 330 340 350
FQTDLRVYLH LRQTWMAFMI ILSILEVVII LLLIFLRKRI LIAIALIKEA
360 370 380 390 400
SRAVGHVMCS LLYPLVTFFL LCLCIAYWAS TSVFLSTSNT AVYKVVDDTA
410 420 430 440 450
CPLLRKTCNP ETFPLRNESL QCPTARCQFA FYGGESTYHR ALLGLQIFNA
460 470 480 490 500
FMFFWLANFV LALGQVTLAG AFASYYWAMR KPDDMPAFPL FSAFGRALRY
510 520 530 540 550
HTGSLAFGSL ILAIVQIIRV MLEYLDQRLK AAQNKFAKFL MVCLKCCFWC
560 570 580 590 600
LEKFIKFLNR NAYIMIAIYG TNFCTSARNA FFLLMRNIIR VAVLDKVTDF
610 620 630 640 650
LFLLGKLLIV GSVGILAFFF FTHRIRIVQD TAPPLNYYWV PILTVIIGSY
660 670 680 690 700
LIAHGFFSVY GMCVDTLFLC FLEDLERNDG SAERPYFMSS TLKKLLNKTN

KKVAES
Length:706
Mass (Da):80,110
Last modified:September 21, 2011 - v2
Checksum:i954AFD393C69A009
GO
Isoform 2 (identifier: Q8BY89-2) [UniParc]FASTAAdd to basket

Also known as: CTL2b, CTL2P1

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MGKDSQNYYGKH → MEDDRKDAVY

Note: Produced by alternative promoter usage.

Show »
Length:704
Mass (Da):79,924
Checksum:i17E13C468805E580
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212MGKDS…YYGKH → MEDDRKDAVY in isoform 2. 2 PublicationsVSP_015432Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041533 mRNA. Translation: BAC30976.1.
AK048648 mRNA. Translation: BAC33409.1.
BC031535 mRNA. Translation: AAH31535.1.
CCDSiCCDS22903.1. [Q8BY89-1]
RefSeqiNP_001186115.1. NM_001199186.1. [Q8BY89-2]
NP_690021.1. NM_152808.3. [Q8BY89-1]
UniGeneiMm.148425.

Genome annotation databases

EnsembliENSMUST00000034697; ENSMUSP00000034697; ENSMUSG00000057193. [Q8BY89-1]
GeneIDi68682.
KEGGimmu:68682.
UCSCiuc009okz.2. mouse. [Q8BY89-2]
uc009ola.2. mouse. [Q8BY89-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041533 mRNA. Translation: BAC30976.1.
AK048648 mRNA. Translation: BAC33409.1.
BC031535 mRNA. Translation: AAH31535.1.
CCDSiCCDS22903.1. [Q8BY89-1]
RefSeqiNP_001186115.1. NM_001199186.1. [Q8BY89-2]
NP_690021.1. NM_152808.3. [Q8BY89-1]
UniGeneiMm.148425.

3D structure databases

ProteinModelPortaliQ8BY89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BY89. 1 interaction.
MINTiMINT-4997673.
STRINGi10090.ENSMUSP00000034697.

Protein family/group databases

TCDBi2.A.92.1.2. the choline transporter-like (ctl) family.

PTM databases

PhosphoSiteiQ8BY89.

Proteomic databases

MaxQBiQ8BY89.
PaxDbiQ8BY89.
PRIDEiQ8BY89.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034697; ENSMUSP00000034697; ENSMUSG00000057193. [Q8BY89-1]
GeneIDi68682.
KEGGimmu:68682.
UCSCiuc009okz.2. mouse. [Q8BY89-2]
uc009ola.2. mouse. [Q8BY89-1]

Organism-specific databases

CTDi57153.
MGIiMGI:1915932. Slc44a2.

Phylogenomic databases

eggNOGiNOG310633.
GeneTreeiENSGT00550000074521.
HOGENOMiHOG000007110.
HOVERGENiHBG062155.
InParanoidiQ8BY89.
KOiK15377.
OMAiENRFAKF.
OrthoDBiEOG7FR7G4.
TreeFamiTF313325.

Enzyme and pathway databases

ReactomeiREACT_271919. Synthesis of PC.
REACT_346270. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

ChiTaRSiSlc44a2. mouse.
NextBioi327690.
PROiQ8BY89.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BY89.
CleanExiMM_SLC44A2.
GenevestigatoriQ8BY89.

Family and domain databases

InterProiIPR007603. Choline_transptr-like.
[Graphical view]
PANTHERiPTHR12385. PTHR12385. 1 hit.
PfamiPF04515. Choline_transpo. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-187 AND ASN-200.
    Tissue: Myoblast.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-187 AND ASN-200.
  5. "Isoforms, expression, glycosylation, and tissue distribution of CTL2/SLC44A2."
    Kommareddi P.K., Nair T.S., Thang L.V., Galano M.M., Babu E., Ganapathy V., Kanazawa T., McHugh J.B., Carey T.E.
    Protein J. 29:417-426(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ISOFORMS 1 AND 3, ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY, GLYCOSYLATION.

Entry informationi

Entry nameiCTL2_MOUSE
AccessioniPrimary (citable) accession number: Q8BY89
Secondary accession number(s): Q8K2F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: September 21, 2011
Last modified: April 1, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.