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Protein

Ubiquitin carboxyl-terminal hydrolase 47

Gene

Usp47

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197NucleophilePROSITE-ProRule annotation1
Active sitei503Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: UniProtKB
  • cellular response to DNA damage stimulus Source: UniProtKB
  • cellular response to UV Source: UniProtKB
  • monoubiquitinated protein deubiquitination Source: UniProtKB
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • negative regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of cell growth Source: UniProtKB
  • response to drug Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processDNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.055

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 47 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 47
Ubiquitin thioesterase 47
Ubiquitin-specific-processing protease 47
Gene namesi
Name:Usp47
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1922246 Usp47

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806771 – 1376Ubiquitin carboxyl-terminal hydrolase 47Add BLAST1376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122N6-acetyllysineBy similarity1
Modified residuei832PhosphoserineBy similarity1
Modified residuei911PhosphoserineCombined sources1
Modified residuei934PhosphoserineCombined sources1
Modified residuei1014PhosphoserineCombined sources1
Modified residuei1016PhosphothreonineCombined sources1
Modified residuei1018PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BY87
PeptideAtlasiQ8BY87
PRIDEiQ8BY87

PTM databases

iPTMnetiQ8BY87
PhosphoSitePlusiQ8BY87

Expressioni

Gene expression databases

BgeeiENSMUSG00000059263
CleanExiMM_USP47
ExpressionAtlasiQ8BY87 baseline and differential
GenevisibleiQ8BY87 MM

Interactioni

Subunit structurei

Interacts with BTRC and FBXW11. Interacts with POLB (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217138, 1 interactor
IntActiQ8BY87, 1 interactor
MINTiQ8BY87
STRINGi10090.ENSMUSP00000102264

Structurei

3D structure databases

ProteinModelPortaliQ8BY87
SMRiQ8BY87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini188 – 564USPAdd BLAST377

Sequence similaritiesi

Belongs to the peptidase C19 family. USP47 subfamily.Curated

Phylogenomic databases

eggNOGiKOG4598 Eukaryota
COG5077 LUCA
GeneTreeiENSGT00760000119158
HOVERGENiHBG058854
InParanoidiQ8BY87
KOiK11857
OMAiMAFTDSH
OrthoDBiEOG091G0173
PhylomeDBiQ8BY87
TreeFamiTF314142

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF14560 Ubiquitin_2, 1 hit
PF00443 UCH, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BY87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPGEENQLV PKEDVFWRCR QNIFDEMKKK FLQIENAAEE PRVLCIIQDT
60 70 80 90 100
TNSKTVSERI TLNLPASTPV RKLFEDVANK VGYINGTFDL TRENGVTTAD
110 120 130 140 150
MAPLDHTSDK SLLDANFEPG KKNFLHLTDK DGEPPQMLLE DSNNVDDSVH
160 170 180 190 200
DRFIGPLPRE GSVASTNDYV SQNYSYSSIL NKSETGYVGL VNQAMTCYLN
210 220 230 240 250
SLLQTLFMTP EFRNALYKWE FEDSEEDPVT SIPYQLQRLF VLLQTSKKRA
260 270 280 290 300
IETTDVTRSF GWDSSEAWQQ HDVQELCRVM FDALEQKWKQ TEQADLINEL
310 320 330 340 350
YQGKLKDYVR CLECGYEGWR IDTYLDIPLV IRPYGSSQAF ASVEEALHAF
360 370 380 390 400
IQPEILDGPN QYFCERCKKK CDARKGLRFL HFPYLLTLQL KRFDFDYTTM
410 420 430 440 450
HRIKLNDRMS FPEELDMSTF IDIEDEKSPQ TESCTDSGAE NEGSCHSDQM
460 470 480 490 500
SNDFSTDDAV DEGICLESSS GSEKISKPGL EKNSLMYELF SVMVHSGSAA
510 520 530 540 550
GGHYYACIKS FSDDQWYSFN DQHVSRITQE DIKKTHGGSS GSRGYYSSAF
560 570 580 590 600
ASSTNAYMLI YRLKDPTRNA KFLEVDEYPE HIKNLVQKER ELEEQEKRQR
610 620 630 640 650
EIERNTCKIK LFCLHPVKQV MMENKLEVHK DKTLKEAVEM AYKMMDLEDV
660 670 680 690 700
IPLDCCRLVK YDEFHDYLER SYEGEEDTPM GLLLGGVKST YMFDLLLETR
710 720 730 740 750
KPDQIFQSYK PGEVMVKVHV VDLKAETVAA PVTVRAYLNQ TVTEFKQLIS
760 770 780 790 800
KATHLPADSM RIVLERCYND LRLLSMPSKT LKAEGFFRSN KVFVESSETV
810 820 830 840 850
DHQAAFTDSH LWKLLDRHAN TIRLFVLLPE QSPGSYSKRT AYQKAGGDSG
860 870 880 890 900
NVDDDCERVK GPAGNLKSVD AILEESTEKL KSLSLQQQQQ DGDNGDSSKS
910 920 930 940 950
TETSDFENIE SPLNERGSST SVDNRELEQH IQTSDPENFQ SEERSDSDVN
960 970 980 990 1000
NDRSTSSVDS DILSSSHSSD TLCNADSAQI PLANGLDSHS ITSSRRTKAN
1010 1020 1030 1040 1050
EGKKETWDTA EEDSGTDSEY DESGKSRGDM QYMYFKADPY TADEGSGEGH
1060 1070 1080 1090 1100
KWLMVHVDKR ITLAAFKQHL EPFVGVLSSH FKVFRVYTSN QEFETVRLNE
1110 1120 1130 1140 1150
TLSSFSDDNK ITIRLGRALK KGEYRVKVCQ LLVNEQEPCK FLLDAVFAKG
1160 1170 1180 1190 1200
MTVRQSKEEL IPQLREQCGL DLSIDRFRLR KKTWKNPGTV FLDYHIYEED
1210 1220 1230 1240 1250
INISSNWEVF LEVLDGVEKM KSMSQLAILT RRWRPAEMKL DPFQELVLES
1260 1270 1280 1290 1300
NSVDELREKL SEISGIPLED IEFAKGRGTF PCDISVLDIH QDLDWNPKVS
1310 1320 1330 1340 1350
TLNVWPLYIC DDGAVIFYRD RTEEVMELTD EQRNELMKKE SSRLQKTGHR
1360 1370
VTYSPRKEKA LKIYLDGAPN KDVAQD
Length:1,376
Mass (Da):157,455
Last modified:July 5, 2005 - v2
Checksum:i09729C3B691C3709
GO
Isoform 2 (identifier: Q8BY87-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-33: Missing.

Note: No experimental confirmation available.
Show »
Length:1,356
Mass (Da):154,841
Checksum:i8DB6A4751B43840C
GO

Sequence cautioni

The sequence BAC27179 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC27195 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC38411 differs from that shown. Reason: Frameshift at position 109.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341A → G in BAC38411 (PubMed:16141072).Curated1
Sequence conflicti376 – 379GLRF → VRDL in BAC38411 (PubMed:16141072).Curated4
Sequence conflicti562R → G in AAI08426 (PubMed:15489334).Curated1
Sequence conflicti1174I → T in AAI08426 (PubMed:15489334).Curated1
Sequence conflicti1178R → H in BAC27195 (PubMed:16141072).Curated1
Sequence conflicti1200 – 1203DINI → IYNF in BAC40073 (PubMed:16141072).Curated4
Sequence conflicti1257R → Q in BAC27195 (PubMed:16141072).Curated1
Sequence conflicti1299 – 1301VST → TRP in AAH12722 (PubMed:15489334).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01441614 – 33Missing in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030909 mRNA Translation: BAC27179.1 Different initiation.
AK030963 mRNA Translation: BAC27195.1 Different initiation.
AK041541 mRNA Translation: BAC30979.1
AK082114 mRNA Translation: BAC38411.1 Frameshift.
AK087981 mRNA Translation: BAC40073.1
AK131138 mRNA Translation: BAD21388.1
BC012722 mRNA Translation: AAH12722.1
BC024117 mRNA Translation: AAH24117.1
BC089364 mRNA Translation: AAH89364.1
BC108425 mRNA Translation: AAI08426.1
CCDSiCCDS40089.1 [Q8BY87-2]
CCDS85384.1 [Q8BY87-1]
RefSeqiNP_598519.2, NM_133758.3 [Q8BY87-2]
NP_796223.2, NM_177249.3 [Q8BY87-1]
UniGeneiMm.16974

Genome annotation databases

EnsembliENSMUST00000106653; ENSMUSP00000102264; ENSMUSG00000059263 [Q8BY87-2]
ENSMUST00000210309; ENSMUSP00000147619; ENSMUSG00000059263 [Q8BY87-1]
GeneIDi74996
KEGGimmu:74996
UCSCiuc009jgg.2 mouse [Q8BY87-2]
uc009jgh.2 mouse [Q8BY87-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP47_MOUSE
AccessioniPrimary (citable) accession number: Q8BY87
Secondary accession number(s): Q32NY0
, Q5EBP2, Q6KAR9, Q80V06, Q8BHU1, Q8BI15, Q8BI16, Q8BUW4, Q91X25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: April 25, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health