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Q8BY02 (NKRF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NF-kappa-B-repressing factor

Short name=NFkB-repressing factor
Alternative name(s):
Transcription factor NRF
Gene names
Name:Nkrf
Synonyms:Nrf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription By similarity.

Subunit structure

Interacts with NF-kappa-B By similarity.

Subcellular location

Nucleusnucleolus By similarity.

Sequence similarities

Contains 1 G-patch domain.

Contains 1 R3H domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 690690NF-kappa-B-repressing factor
PRO_0000096870

Regions

Domain551 – 59646G-patch
Domain600 – 66465R3H
DNA binding296 – 38893 By similarity
Region1 – 296296Active repression domain By similarity
Motif25 – 4521Nuclear localization signal By similarity

Experimental info

Sequence conflict6861M → V in BAC37550. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BY02 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 0A99E1141183F999

FASTA69077,739
        10         20         30         40         50         60 
MEKILHMAEG IDIGEMPSYD LMLPKPSKGQ KRYLSTYDGQ NPPKKQAGSK FHVRARFEPV 

        70         80         90        100        110        120 
HFVASSSKAE RQEDPYGPQT KDVNGRTHFA SMPRNFYQDY TQDSFSIQDG NSQYCNSSGF 

       130        140        150        160        170        180 
IFTKDQPVAT NMYFDSGNPA PSSTSQQANC QPAPEPPPSQ MYPESLVAEK QYFIEKLTAT 

       190        200        210        220        230        240 
IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY 

       250        260        270        280        290        300 
ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHI IGEDLVVCQI 

       310        320        330        340        350        360 
GMLSYEFPPA LKPPEDLVVL GKDASGQPIF NSSAKHWTNF VITENANDAI GILNNSASFN 

       370        380        390        400        410        420 
KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKVLQ KTQPTYPSVK 

       430        440        450        460        470        480 
SSQCHSGSSP KGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC 

       490        500        510        520        530        540 
KVILESEVIA EAVGVKKSVK YEAAGEAVKT LKKTQPTVIN NLKKGTVEDV ISRNEIQGRS 

       550        560        570        580        590        600 
AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK 

       610        620        630        640        650        660 
IAKRDIEQII RNYARSESHS DLTFSTELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV 

       670        680        690 
GRKRRKEDLL DQLKQEGQVG HYELVMPQAN 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum and Embryo.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK020455 mRNA. Translation: BAB32107.1.
AK042690 mRNA. Translation: BAC31334.1.
AK079123 mRNA. Translation: BAC37550.1.
AL450399 Genomic DNA. Translation: CAM19779.1.
BC138828 mRNA. Translation: AAI38829.1.
BC138829 mRNA. Translation: AAI38830.1.
CCDSCCDS30065.1.
RefSeqNP_084167.2. NM_029891.2.
UniGeneMm.21649.

3D structure databases

ProteinModelPortalQ8BY02.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid218599. 3 interactions.

PTM databases

PhosphoSiteQ8BY02.

Proteomic databases

PaxDbQ8BY02.
PRIDEQ8BY02.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
GeneID77286.
KEGGmmu:77286.
UCSCuc009sxw.1. mouse.

Organism-specific databases

CTD55922.
MGIMGI:1924536. Nkrf.

Phylogenomic databases

eggNOGNOG123689.
GeneTreeENSGT00530000063234.
HOGENOMHOG000230930.
HOVERGENHBG052630.
InParanoidA2A3V7.
OMAWRYSHES.
OrthoDBEOG7XWPN1.
TreeFamTF326321.

Gene expression databases

BgeeQ8BY02.
CleanExMM_NKRF.
GenevestigatorQ8BY02.

Family and domain databases

Gene3D3.30.1370.50. 1 hit.
3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view]
PfamPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMSSF82708. SSF82708. 1 hit.
PROSITEPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNKRF. mouse.
NextBio346701.
PROQ8BY02.
SOURCESearch...

Entry information

Entry nameNKRF_MOUSE
AccessionPrimary (citable) accession number: Q8BY02
Secondary accession number(s): A2A3V7, Q8BJU5, Q9CRL2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot