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Protein

NF-kappa-B-repressing factor

Gene

Nkrf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi296 – 38893By similarityAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-repressing factor
Short name:
NFkB-repressing factor
Alternative name(s):
Transcription factor NRF
Gene namesi
Name:Nkrf
Synonyms:Nrf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1924536. Nkrf.

Subcellular locationi

Nucleusnucleolus By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. nucleolus Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690NF-kappa-B-repressing factorPRO_0000096870Add
BLAST

Proteomic databases

MaxQBiQ8BY02.
PaxDbiQ8BY02.
PRIDEiQ8BY02.

PTM databases

PhosphoSiteiQ8BY02.

Expressioni

Gene expression databases

BgeeiQ8BY02.
CleanExiMM_NKRF.
GenevestigatoriQ8BY02.

Interactioni

Subunit structurei

Interacts with NF-kappa-B.By similarity

Protein-protein interaction databases

BioGridi218599. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BY02.
SMRiQ8BY02. Positions 347-412, 437-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 59646G-patchPROSITE-ProRule annotationAdd
BLAST
Domaini600 – 66465R3HPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Active repression domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi25 – 4521Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 G-patch domain.PROSITE-ProRule annotation
Contains 1 R3H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG123689.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiQ8BY02.
OMAiWRYSHES.
OrthoDBiEOG7XWPN1.
TreeFamiTF326321.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BY02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKILHMAEG IDIGEMPSYD LMLPKPSKGQ KRYLSTYDGQ NPPKKQAGSK
60 70 80 90 100
FHVRARFEPV HFVASSSKAE RQEDPYGPQT KDVNGRTHFA SMPRNFYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCNSSGF IFTKDQPVAT NMYFDSGNPA PSSTSQQANC
160 170 180 190 200
QPAPEPPPSQ MYPESLVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHI IGEDLVVCQI
310 320 330 340 350
GMLSYEFPPA LKPPEDLVVL GKDASGQPIF NSSAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKVLQ KTQPTYPSVK SSQCHSGSSP KGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKSVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGTVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHS DLTFSTELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Length:690
Mass (Da):77,739
Last modified:July 27, 2011 - v3
Checksum:i0A99E1141183F999
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti686 – 6861M → V in BAC37550 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020455 mRNA. Translation: BAB32107.1.
AK042690 mRNA. Translation: BAC31334.1.
AK079123 mRNA. Translation: BAC37550.1.
AL450399 Genomic DNA. Translation: CAM19779.1.
BC138828 mRNA. Translation: AAI38829.1.
BC138829 mRNA. Translation: AAI38830.1.
CCDSiCCDS30065.1.
RefSeqiNP_084167.2. NM_029891.2.
UniGeneiMm.21649.

Genome annotation databases

EnsembliENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
GeneIDi77286.
KEGGimmu:77286.
UCSCiuc009sxw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020455 mRNA. Translation: BAB32107.1.
AK042690 mRNA. Translation: BAC31334.1.
AK079123 mRNA. Translation: BAC37550.1.
AL450399 Genomic DNA. Translation: CAM19779.1.
BC138828 mRNA. Translation: AAI38829.1.
BC138829 mRNA. Translation: AAI38830.1.
CCDSiCCDS30065.1.
RefSeqiNP_084167.2. NM_029891.2.
UniGeneiMm.21649.

3D structure databases

ProteinModelPortaliQ8BY02.
SMRiQ8BY02. Positions 347-412, 437-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218599. 3 interactions.

PTM databases

PhosphoSiteiQ8BY02.

Proteomic databases

MaxQBiQ8BY02.
PaxDbiQ8BY02.
PRIDEiQ8BY02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
GeneIDi77286.
KEGGimmu:77286.
UCSCiuc009sxw.1. mouse.

Organism-specific databases

CTDi55922.
MGIiMGI:1924536. Nkrf.

Phylogenomic databases

eggNOGiNOG123689.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiQ8BY02.
OMAiWRYSHES.
OrthoDBiEOG7XWPN1.
TreeFamiTF326321.

Miscellaneous databases

ChiTaRSiNkrf. mouse.
NextBioi346701.
PROiQ8BY02.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BY02.
CleanExiMM_NKRF.
GenevestigatoriQ8BY02.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 2 hits.
SM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum and Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiNKRF_MOUSE
AccessioniPrimary (citable) accession number: Q8BY02
Secondary accession number(s): A2A3V7, Q8BJU5, Q9CRL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.