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Protein

NF-kappa-B-repressing factor

Gene

Nkrf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi296 – 388By similarityAdd BLAST93

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-repressing factor
Short name:
NFkB-repressing factor
Alternative name(s):
Transcription factor NRF
Gene namesi
Name:Nkrf
Synonyms:Nrf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1924536. Nkrf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968701 – 690NF-kappa-B-repressing factorAdd BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei618PhosphoserineBy similarity1
Cross-linki674Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BY02.
MaxQBiQ8BY02.
PaxDbiQ8BY02.
PeptideAtlasiQ8BY02.
PRIDEiQ8BY02.

PTM databases

PhosphoSitePlusiQ8BY02.

Expressioni

Gene expression databases

BgeeiENSMUSG00000044149.
CleanExiMM_NKRF.
GenevisibleiQ8BY02. MM.

Interactioni

Subunit structurei

Interacts with NF-kappa-B. Interacts with XRN2.By similarity

Protein-protein interaction databases

BioGridi218599. 3 interactors.
IntActiQ8BY02. 1 interactor.
STRINGi10090.ENSMUSP00000061546.

Structurei

3D structure databases

ProteinModelPortaliQ8BY02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 596G-patchPROSITE-ProRule annotationAdd BLAST46
Domaini600 – 664R3HPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Active repression domainBy similarityAdd BLAST296

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi25 – 45Nuclear localization signalBy similarityAdd BLAST21

Sequence similaritiesi

Contains 1 G-patch domain.PROSITE-ProRule annotation
Contains 1 R3H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiQ8BY02.
OMAiVCQIGMP.
OrthoDBiEOG091G0HNZ.
TreeFamiTF326321.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.30.160.20. 2 hits.
InterProiIPR014720. dsRBD_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_dom.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BY02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKILHMAEG IDIGEMPSYD LMLPKPSKGQ KRYLSTYDGQ NPPKKQAGSK
60 70 80 90 100
FHVRARFEPV HFVASSSKAE RQEDPYGPQT KDVNGRTHFA SMPRNFYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCNSSGF IFTKDQPVAT NMYFDSGNPA PSSTSQQANC
160 170 180 190 200
QPAPEPPPSQ MYPESLVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHI IGEDLVVCQI
310 320 330 340 350
GMLSYEFPPA LKPPEDLVVL GKDASGQPIF NSSAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKVLQ KTQPTYPSVK SSQCHSGSSP KGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKSVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGTVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHS DLTFSTELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Length:690
Mass (Da):77,739
Last modified:July 27, 2011 - v3
Checksum:i0A99E1141183F999
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti686M → V in BAC37550 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020455 mRNA. Translation: BAB32107.1.
AK042690 mRNA. Translation: BAC31334.1.
AK079123 mRNA. Translation: BAC37550.1.
AL450399 Genomic DNA. Translation: CAM19779.1.
BC138828 mRNA. Translation: AAI38829.1.
BC138829 mRNA. Translation: AAI38830.1.
CCDSiCCDS30065.1.
RefSeqiNP_084167.2. NM_029891.2.
UniGeneiMm.21649.

Genome annotation databases

EnsembliENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
GeneIDi77286.
KEGGimmu:77286.
UCSCiuc009sxw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020455 mRNA. Translation: BAB32107.1.
AK042690 mRNA. Translation: BAC31334.1.
AK079123 mRNA. Translation: BAC37550.1.
AL450399 Genomic DNA. Translation: CAM19779.1.
BC138828 mRNA. Translation: AAI38829.1.
BC138829 mRNA. Translation: AAI38830.1.
CCDSiCCDS30065.1.
RefSeqiNP_084167.2. NM_029891.2.
UniGeneiMm.21649.

3D structure databases

ProteinModelPortaliQ8BY02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218599. 3 interactors.
IntActiQ8BY02. 1 interactor.
STRINGi10090.ENSMUSP00000061546.

PTM databases

PhosphoSitePlusiQ8BY02.

Proteomic databases

EPDiQ8BY02.
MaxQBiQ8BY02.
PaxDbiQ8BY02.
PeptideAtlasiQ8BY02.
PRIDEiQ8BY02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
GeneIDi77286.
KEGGimmu:77286.
UCSCiuc009sxw.1. mouse.

Organism-specific databases

CTDi55922.
MGIiMGI:1924536. Nkrf.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000230930.
HOVERGENiHBG052630.
InParanoidiQ8BY02.
OMAiVCQIGMP.
OrthoDBiEOG091G0HNZ.
TreeFamiTF326321.

Miscellaneous databases

ChiTaRSiNkrf. mouse.
PROiQ8BY02.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044149.
CleanExiMM_NKRF.
GenevisibleiQ8BY02. MM.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.30.160.20. 2 hits.
InterProiIPR014720. dsRBD_dom.
IPR000467. G_patch_dom.
IPR001374. R3H_dom.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51061. R3H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKRF_MOUSE
AccessioniPrimary (citable) accession number: Q8BY02
Secondary accession number(s): A2A3V7, Q8BJU5, Q9CRL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.