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Protein

Zinc finger and BTB domain-containing protein 49

Gene

Zbtb49

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor. Inhibits cell proliferation by activating either CDKN1A/p21 transcription or RB1 transcription.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri386 – 40823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri442 – 46423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri470 – 49223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri498 – 52023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri526 – 54823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri554 – 57623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 49
Alternative name(s):
Zinc finger protein 509
Gene namesi
Name:Zbtb49
Synonyms:Zfp509, Znf509
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1922329. Zbtb49.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756Zinc finger and BTB domain-containing protein 49PRO_0000047627Add
BLAST

Proteomic databases

PaxDbiQ8BXX2.
PRIDEiQ8BXX2.

PTM databases

iPTMnetiQ8BXX2.
PhosphoSiteiQ8BXX2.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in white adipose tissue and kidney, intermediate levels in brain, liver and heart, and lowest levels in spleen, brown adipose tissue and muscle.1 Publication

Gene expression databases

BgeeiQ8BXX2.
CleanExiMM_ZFP509.
ExpressionAtlasiQ8BXX2. baseline and differential.
GenevisibleiQ8BXX2. MM.

Interactioni

Subunit structurei

Interacts with EP300, KAT5/Tip60 and ZBTB17. The interaction with EP300 is direct and leads to synergistic induction of CDKN1A. On the CDKN1A promoter, forms a complex with ZBTB17; this interaction leads to additive CDKN1A transactivation. The interaction with ZBTB17 may block ZBTB17 repressor activity.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092429.

Structurei

3D structure databases

ProteinModelPortaliQ8BXX2.
SMRiQ8BXX2. Positions 7-150, 383-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9167BTBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri386 – 40823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri442 – 46423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri470 – 49223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri498 – 52023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri526 – 54823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri554 – 57623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129682.
HOGENOMiHOG000140405.
HOVERGENiHBG061540.
InParanoidiQ8BXX2.
OMAiMPVSVKL.
OrthoDBiEOG7KWSH5.
PhylomeDBiQ8BXX2.
TreeFamiTF330993.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BXX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPVAVHSCH LLQQLREQRI QGLLCDCMLV VRGVCFKAHK NVLAAFSQYF
60 70 80 90 100
RSLFQNSSSQ KNDVFHLDVT NVSGIGQILD FMYTSRLDLN QDNIQVMLDT
110 120 130 140 150
AQCLQVQNVL NLCHTFLKSA PAAQLPGLPC AGGFSLQSVA LDGTCAVSEH
160 170 180 190 200
YPPPSLQECP VEGPQAKVPA EVNARAPSAN FSRPTEVSKP DAAGGSCPEL
210 220 230 240 250
PCKQPNHYYK LRTLYSKQYY KQTACPSQVP ATQQPLTRSA STDLAAADSQ
260 270 280 290 300
PPVEGRPAVL ETPEHLPSTF VAPPVRNSGN DSEADPLSEP PAKQMRLKKA
310 320 330 340 350
MHLKKLNFLK SQQSAECTSH PEPDNGLARR EESAAKEDAV ERAGSQTAEE
360 370 380 390 400
KGRGELGPES SREEELPGAP ASWEDPSQAL QPQKQYACEL CGKPFKHPSN
410 420 430 440 450
LELHKRSHTG EKPFECNICG KHFSQAGNLQ THLRRHSGEK PYICEICGKR
460 470 480 490 500
FAASGDVQRH IIIHSGEKPH LCDTCGRGFS NFSNLKEHKK THTADKVFTC
510 520 530 540 550
DECGKSFNMQ RKLVKHRVRH TGERPYSCPA CGKCFGGSGD LRRHVRTHTG
560 570 580 590 600
EKPYSCEVCS KCFTRSAVLR RHKRMHGRAD ARSPVVLGEL SRPIEPSDLD
610 620 630 640 650
RSQSSDSFSQ DVSVTLMPVS VKLPVQPVES SVAGFDGHCS GSYCKLRSML
660 670 680 690 700
RPPGMSDQDR LSLEPSKLAK PPELQSQPQA YAYSDVEPSA GVEQPQADGM
710 720 730 740 750
AVSRSSLATL DNHCTEPLGS RAPSVTYRNS EGQFFSSMTL WGLAMKTLQN

EHELEQ
Length:756
Mass (Da):83,091
Last modified:March 1, 2003 - v1
Checksum:i0D7E6261C61F3225
GO
Isoform 2 (identifier: Q8BXX2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-284: Missing.
     285-424: DPLSEPPAKQ...ECNICGKHFS → MGWPGGRSLL...AIWSCTNGLI

Note: No experimental confirmation available.
Show »
Length:416
Mass (Da):46,283
Checksum:iD2728A6C8D44E502
GO
Isoform 3 (identifier: Q8BXX2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-508: Missing.

Note: No experimental confirmation available.
Show »
Length:248
Mass (Da):27,350
Checksum:i9ACADFB99B33B384
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 508508Missing in isoform 3. 1 PublicationVSP_016344Add
BLAST
Alternative sequencei1 – 284284Missing in isoform 2. 1 PublicationVSP_016345Add
BLAST
Alternative sequencei285 – 424140DPLSE…GKHFS → MGWPGGRSLLLRKMQWRELE ARLLKKKGGENWVQRAAVRR SCQEPQLPGKTRPRLSSPRN SMHVNCVGSLLNTQAIWSCT NGLI in isoform 2. 1 PublicationVSP_016346Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043044 mRNA. Translation: BAC31447.1.
AK140546 mRNA. Translation: BAE24420.1.
BC028988 mRNA. Translation: AAH28988.1.
BC029122 mRNA. Translation: AAH29122.1.
CCDSiCCDS19252.1. [Q8BXX2-1]
RefSeqiNP_083438.1. NM_029162.2. [Q8BXX2-1]
XP_006504228.1. XM_006504165.2. [Q8BXX2-2]
UniGeneiMm.258741.

Genome annotation databases

EnsembliENSMUST00000094833; ENSMUSP00000092429; ENSMUSG00000029127. [Q8BXX2-1]
GeneIDi75079.
KEGGimmu:75079.
UCSCiuc008xgc.1. mouse. [Q8BXX2-1]
uc008xge.1. mouse. [Q8BXX2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043044 mRNA. Translation: BAC31447.1.
AK140546 mRNA. Translation: BAE24420.1.
BC028988 mRNA. Translation: AAH28988.1.
BC029122 mRNA. Translation: AAH29122.1.
CCDSiCCDS19252.1. [Q8BXX2-1]
RefSeqiNP_083438.1. NM_029162.2. [Q8BXX2-1]
XP_006504228.1. XM_006504165.2. [Q8BXX2-2]
UniGeneiMm.258741.

3D structure databases

ProteinModelPortaliQ8BXX2.
SMRiQ8BXX2. Positions 7-150, 383-581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092429.

PTM databases

iPTMnetiQ8BXX2.
PhosphoSiteiQ8BXX2.

Proteomic databases

PaxDbiQ8BXX2.
PRIDEiQ8BXX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094833; ENSMUSP00000092429; ENSMUSG00000029127. [Q8BXX2-1]
GeneIDi75079.
KEGGimmu:75079.
UCSCiuc008xgc.1. mouse. [Q8BXX2-1]
uc008xge.1. mouse. [Q8BXX2-3]

Organism-specific databases

CTDi166793.
MGIiMGI:1922329. Zbtb49.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129682.
HOGENOMiHOG000140405.
HOVERGENiHBG061540.
InParanoidiQ8BXX2.
OMAiMPVSVKL.
OrthoDBiEOG7KWSH5.
PhylomeDBiQ8BXX2.
TreeFamiTF330993.

Miscellaneous databases

PROiQ8BXX2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BXX2.
CleanExiMM_ZFP509.
ExpressionAtlasiQ8BXX2. baseline and differential.
GenevisibleiQ8BXX2. MM.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  3. "Two ZNF509 (ZBTB49) isoforms induce cell-cycle arrest by activating transcription of p21/CDKN1A and RB upon exposure to genotoxic stress."
    Jeon B.N., Kim M.K., Yoon J.H., Kim M.Y., An H., Noh H.J., Choi W.I., Koh D.I., Hur M.W.
    Nucleic Acids Res. 42:11447-11461(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiZBT49_MOUSE
AccessioniPrimary (citable) accession number: Q8BXX2
Secondary accession number(s): Q8CID4, Q8K2Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.