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Protein

Aminopeptidase O

Gene

Aopep

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Strongly inhibited by ophenanthroline, a metalloprotease inhibitor. Inhibited by arphamenine A, a potent inhibitor of aminopeptidases. Not affected by AEBSF and E-64, which are common inhibitors of serine- and cysteine-proteases, respectively (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi480Zinc; catalyticBy similarity1
Active sitei481Proton acceptorBy similarity1
Metal bindingi484Zinc; catalyticBy similarity1
Metal bindingi503Zinc; catalyticBy similarity1
Sitei587Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • metalloaminopeptidase activity Source: GO_Central
  • metallopeptidase activity Source: UniProtKB
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • peptide catabolic process Source: GO_Central
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.028.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase O (EC:3.4.11.-)
Short name:
AP-O
Gene namesi
Name:Aopep
Synonyms:Onpep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919311. 2010111I01Rik.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleolus Source: MGI
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950921 – 817Aminopeptidase OAdd BLAST817

Proteomic databases

PaxDbiQ8BXQ6.
PRIDEiQ8BXQ6.

PTM databases

iPTMnetiQ8BXQ6.
PhosphoSitePlusiQ8BXQ6.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Developmental stagei

Expressed from day 23 to day 42, reaching a peak in testis from 30-35-day old mice, when mice reach sexual maturity.1 Publication

Gene expression databases

CleanExiMM_2010111I01RIK.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089148.

Structurei

3D structure databases

ProteinModelPortaliQ8BXQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
HOVERGENiHBG050496.
InParanoidiQ8BXQ6.
KOiK09606.

Family and domain databases

InterProiIPR033577. AOPep.
IPR016024. ARM-type_fold.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 4 hits.
PTHR11533:SF175. PTHR11533:SF175. 4 hits.
PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 2 hits.
[Graphical view]
SMARTiSM01263. Leuk-A4-hydro_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BXQ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIKLDPSRD DLPLMANTSH MLVKHYILDL DVDFGNQVIE GNIVLFFGDG
60 70 80 90 100
NRFKNQSRST QETFQMESEE ADIFRTAEPC HVPEMDSSTF SPKMGHRECA
110 120 130 140 150
VCGKGDQDAF DNDGNHDNQE RDSEISSSKY CCDTGNHGKR DFLLVLDCCD
160 170 180 190 200
LSVLKVEEVD VAAVPGLEKF TKAPKLLATP EKLRCEIVRD LVALPADAWR
210 220 230 240 250
EQLDCYTRCS QAPGCGELLI DSDNWSLRIR KTGTSTPADF PRAIRIWYKT
260 270 280 290 300
KPEGQSVAWT TDQNGRPCVY TMGSPINNRA LFPCQEPPVA MSTWQATVRA
310 320 330 340 350
AASFVVLMSG ENSAKPTPLR ERSWHYYVTM PMPASTFAIA VGCWTEMKPK
360 370 380 390 400
ASPPDDLMTE HSLPLSPSEA DLRFVFGDCN HMEYPCRFQS ASAASQDIIP
410 420 430 440 450
YRVFAPVCLE GACQEALLWL IPSCLSAAHS VLGTHPFSRL DILIVPTNFP
460 470 480 490 500
SLGMASPHII FLSQSTLTGT SHLCGTRLCH EIAHSWFGLA IGARDWTEEW
510 520 530 540 550
LSEGFATHLE DIFWAEAQQL PPHEALEQQE LRACLRWHRL QDELRNSPEG
560 570 580 590 600
MQVLRPNKEE TGHVSASGAS VVKHGLNPEK GFMQVHYLKG YFLLRFLTRT
610 620 630 640 650
LGEKIYFPFL RKFVHLFHGQ LILSQDFLQM LLENIPENKR LGLSVENIVR
660 670 680 690 700
DWLECSGIPK ALQEERKAED CSPSRLARQV AKWIRVNRRP RKRKRGKREV
710 720 730 740 750
AFELSPDQIV LLLEWLLEQK TLSPQTLHCL QQTYHLPEQD AEVRHRWCEL
760 770 780 790 800
VIKHKYTKAY NQVERFLLED QAMGIYLYGE LMVSEDARLQ QLAHRCFELV
810
KEHMDRASAQ VVTEMLF
Length:817
Mass (Da):92,955
Last modified:March 15, 2005 - v2
Checksum:i4E2D3C79A33B63EC
GO
Isoform 2 (identifier: Q8BXQ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     680-680: V → VGSEV
     703-703: E → EK

Show »
Length:822
Mass (Da):93,456
Checksum:i867590EA0C4C5ED9
GO
Isoform 3 (identifier: Q8BXQ6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     322-322: R → GYM
     374-378: FVFGD → YDNT
     456-472: SPHIIFLSQSTLTGTSH → RYAAHHGMWNMLKMSRN
     473-817: Missing.

Note: No experimental confirmation available.
Show »
Length:473
Mass (Da):52,774
Checksum:i0EC30CA580F2CD97
GO

Sequence cautioni

The sequence BAC36293 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013166322R → GYM in isoform 3. 1 Publication1
Alternative sequenceiVSP_013167374 – 378FVFGD → YDNT in isoform 3. 1 Publication5
Alternative sequenceiVSP_013168456 – 472SPHII…TGTSH → RYAAHHGMWNMLKMSRN in isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_013169473 – 817Missing in isoform 3. 1 PublicationAdd BLAST345
Alternative sequenceiVSP_013170680V → VGSEV in isoform 2. 2 Publications1
Alternative sequenceiVSP_013171703E → EK in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ810420 mRNA. Translation: CAH17902.1.
AK044477 mRNA. Translation: BAC31943.1.
AK076298 mRNA. Translation: BAC36293.1. Different initiation.
BC064132 mRNA. Translation: AAH64132.1.
BC065422 mRNA. Translation: AAH65422.1.
RefSeqiNP_001276853.1. NM_001289924.1.
NP_001276855.1. NM_001289926.1.
UniGeneiMm.188105.
Mm.491169.

Genome annotation databases

GeneIDi72061.
KEGGimmu:72061.
UCSCiuc007qxh.1. mouse. [Q8BXQ6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ810420 mRNA. Translation: CAH17902.1.
AK044477 mRNA. Translation: BAC31943.1.
AK076298 mRNA. Translation: BAC36293.1. Different initiation.
BC064132 mRNA. Translation: AAH64132.1.
BC065422 mRNA. Translation: AAH65422.1.
RefSeqiNP_001276853.1. NM_001289924.1.
NP_001276855.1. NM_001289926.1.
UniGeneiMm.188105.
Mm.491169.

3D structure databases

ProteinModelPortaliQ8BXQ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089148.

Protein family/group databases

MEROPSiM01.028.

PTM databases

iPTMnetiQ8BXQ6.
PhosphoSitePlusiQ8BXQ6.

Proteomic databases

PaxDbiQ8BXQ6.
PRIDEiQ8BXQ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72061.
KEGGimmu:72061.
UCSCiuc007qxh.1. mouse. [Q8BXQ6-3]

Organism-specific databases

MGIiMGI:1919311. 2010111I01Rik.

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
HOVERGENiHBG050496.
InParanoidiQ8BXQ6.
KOiK09606.

Miscellaneous databases

ChiTaRSi2010111I01Rik. mouse.
PROiQ8BXQ6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_2010111I01RIK.

Family and domain databases

InterProiIPR033577. AOPep.
IPR016024. ARM-type_fold.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 4 hits.
PTHR11533:SF175. PTHR11533:SF175. 4 hits.
PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 2 hits.
[Graphical view]
SMARTiSM01263. Leuk-A4-hydro_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMPO_MOUSE
AccessioniPrimary (citable) accession number: Q8BXQ6
Secondary accession number(s): Q6P0W6, Q6P394, Q8BHX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.