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Q8BXQ2 (PIGT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI transamidase component PIG-T
Alternative name(s):
Neuronal development-associated protein 7
Phosphatidylinositol-glycan biosynthesis class T protein
Gene names
Name:Pigt
Synonyms:Ndap7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates By similarity.

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure

Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Post-translational modification

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity By similarity.

Sequence similarities

Belongs to the PIGT family.

Sequence caution

The sequence AAH34175.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAK32809.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB39711.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC34729.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 582557GPI transamidase component PIG-T
PRO_0000024108

Regions

Topological domain26 – 525500Lumenal Potential
Transmembrane526 – 54823Helical; Potential
Topological domain549 – 58234Cytoplasmic Potential

Amino acid modifications

Glycosylation1681N-linked (GlcNAc...) Ref.5
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Disulfide bond186Interchain (with C-92 in PIGK/GPI8) By similarity

Experimental info

Sequence conflict3461P → Q in BAC31951. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BXQ2 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 39FF3CF98D3F9322

FASTA58265,705
        10         20         30         40         50         60 
MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA TFQFRTRWDS 

        70         80         90        100        110        120 
DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT RYWGPPFLQA PSGAELWVWF 

       130        140        150        160        170        180 
QDTVTDVDKS WRELSNVLSG IFCASLNFID ATNTVTPTAS FKPLGLANDT DDYFLRYAVL 

       190        200        210        220        230        240 
PREVVCTENL TPWKKLLPCS SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW 

       250        260        270        280        290        300 
ELRQTLSVVF DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV 

       310        320        330        340        350        360 
SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA LPVPFLHAQR 

       370        380        390        400        410        420 
YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY VHTLTITSKG KENKPSYIHY 

       430        440        450        460        470        480 
QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF ERALLKWTEY TPDPNHGFYV SPSVLSALVP 

       490        500        510        520        530        540 
SVVAAKPVDW EGSPLFNTLF PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV 

       550        560        570        580 
AVCYGSFYNL LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Retina.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Cloning and identification of differentially expressed transcripts in primary culture of GABAergic neurons."
Li Z., Li Q., Sun C.X., Hertz L., Yu A.C.H.
Neurochem. Res. 26:1101-1105(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-582.
Strain: ICR.
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-582.
Tissue: Mammary tumor.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK044499 mRNA. Translation: BAC31951.1.
AK155057 mRNA. Translation: BAE33018.1.
AL591478 Genomic DNA. Translation: CAM15012.2.
AJ308886 mRNA. Translation: CAC34729.1. Different initiation.
AF361435 mRNA. Translation: AAK32809.1. Different initiation.
AB057593 mRNA. Translation: BAB39711.1. Different initiation.
BC034175 mRNA. Translation: AAH34175.1. Different initiation.
RefSeqNP_598540.2. NM_133779.2.
UniGeneMm.28228.

3D structure databases

ProteinModelPortalQ8BXQ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BXQ2. 1 interaction.
MINTMINT-4107578.

PTM databases

PhosphoSiteQ8BXQ2.

Proteomic databases

PaxDbQ8BXQ2.
PRIDEQ8BXQ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
GeneID78928.
KEGGmmu:78928.
UCSCuc008nvc.1. mouse.

Organism-specific databases

CTD51604.
MGIMGI:1926178. Pigt.

Phylogenomic databases

eggNOGNOG331717.
GeneTreeENSGT00390000018558.
HOGENOMHOG000215945.
HOVERGENHBG049395.
InParanoidA2A5G2.
KOK05292.
OMATLEVNPP.
OrthoDBEOG70PBXH.
PhylomeDBQ8BXQ2.
TreeFamTF105921.

Enzyme and pathway databases

UniPathwayUPA00196.

Gene expression databases

ArrayExpressQ8BXQ2.
BgeeQ8BXQ2.
GenevestigatorQ8BXQ2.

Family and domain databases

InterProIPR007245. PIG-T.
[Graphical view]
PANTHERPTHR12959. PTHR12959. 1 hit.
PfamPF04113. Gpi16. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio349782.
PROQ8BXQ2.
SOURCESearch...

Entry information

Entry namePIGT_MOUSE
AccessionPrimary (citable) accession number: Q8BXQ2
Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot