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Q8BXQ2

- PIGT_MOUSE

UniProt

Q8BXQ2 - PIGT_MOUSE

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Protein
GPI transamidase component PIG-T
Gene
Pigt, Ndap7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates By similarity.

Pathwayi

GO - Biological processi

  1. attachment of GPI anchor to protein Source: UniProtKB
  2. neuron apoptotic process Source: MGI
  3. neuron differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI transamidase component PIG-T
Alternative name(s):
Neuronal development-associated protein 7
Phosphatidylinositol-glycan biosynthesis class T protein
Gene namesi
Name:Pigt
Synonyms:Ndap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1926178. Pigt.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 525500Lumenal Reviewed prediction
Add
BLAST
Transmembranei526 – 54823Helical; Reviewed prediction
Add
BLAST
Topological domaini549 – 58234Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. GPI-anchor transamidase complex Source: UniProtKB
  2. cytoplasm Source: MGI
  3. cytoplasmic membrane-bounded vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 582557GPI transamidase component PIG-T
PRO_0000024108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication
Disulfide bondi186 – 186Interchain (with C-92 in PIGK/GPI8) By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BXQ2.
PaxDbiQ8BXQ2.
PRIDEiQ8BXQ2.

PTM databases

PhosphoSiteiQ8BXQ2.

Expressioni

Gene expression databases

ArrayExpressiQ8BXQ2.
BgeeiQ8BXQ2.
GenevestigatoriQ8BXQ2.

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS By similarity.

Protein-protein interaction databases

IntActiQ8BXQ2. 1 interaction.
MINTiMINT-4107578.

Structurei

3D structure databases

ProteinModelPortaliQ8BXQ2.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGT family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG331717.
GeneTreeiENSGT00390000018558.
HOGENOMiHOG000215945.
HOVERGENiHBG049395.
InParanoidiA2A5G2.
KOiK05292.
OMAiTLEVNPP.
OrthoDBiEOG70PBXH.
PhylomeDBiQ8BXQ2.
TreeFamiTF105921.

Family and domain databases

InterProiIPR007245. PIG-T.
[Graphical view]
PANTHERiPTHR12959. PTHR12959. 1 hit.
PfamiPF04113. Gpi16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BXQ2-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA    50
TFQFRTRWDS DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT 100
RYWGPPFLQA PSGAELWVWF QDTVTDVDKS WRELSNVLSG IFCASLNFID 150
ATNTVTPTAS FKPLGLANDT DDYFLRYAVL PREVVCTENL TPWKKLLPCS 200
SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW ELRQTLSVVF 250
DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV 300
SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA 350
LPVPFLHAQR YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY 400
VHTLTITSKG KENKPSYIHY QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF 450
ERALLKWTEY TPDPNHGFYV SPSVLSALVP SVVAAKPVDW EGSPLFNTLF 500
PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV AVCYGSFYNL 550
LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL 582
Length:582
Mass (Da):65,705
Last modified:March 1, 2004 - v2
Checksum:i39FF3CF98D3F9322
GO

Sequence cautioni

The sequence AAH34175.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAK32809.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB39711.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAC34729.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti346 – 3461P → Q in BAC31951. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK044499 mRNA. Translation: BAC31951.1.
AK155057 mRNA. Translation: BAE33018.1.
AL591478 Genomic DNA. Translation: CAM15012.2.
AJ308886 mRNA. Translation: CAC34729.1. Different initiation.
AF361435 mRNA. Translation: AAK32809.1. Different initiation.
AB057593 mRNA. Translation: BAB39711.1. Different initiation.
BC034175 mRNA. Translation: AAH34175.1. Different initiation.
CCDSiCCDS17038.2.
RefSeqiNP_598540.2. NM_133779.2.
UniGeneiMm.28228.

Genome annotation databases

EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
GeneIDi78928.
KEGGimmu:78928.
UCSCiuc008nvc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK044499 mRNA. Translation: BAC31951.1 .
AK155057 mRNA. Translation: BAE33018.1 .
AL591478 Genomic DNA. Translation: CAM15012.2 .
AJ308886 mRNA. Translation: CAC34729.1 . Different initiation.
AF361435 mRNA. Translation: AAK32809.1 . Different initiation.
AB057593 mRNA. Translation: BAB39711.1 . Different initiation.
BC034175 mRNA. Translation: AAH34175.1 . Different initiation.
CCDSi CCDS17038.2.
RefSeqi NP_598540.2. NM_133779.2.
UniGenei Mm.28228.

3D structure databases

ProteinModelPortali Q8BXQ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BXQ2. 1 interaction.
MINTi MINT-4107578.

PTM databases

PhosphoSitei Q8BXQ2.

Proteomic databases

MaxQBi Q8BXQ2.
PaxDbi Q8BXQ2.
PRIDEi Q8BXQ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000103101 ; ENSMUSP00000099390 ; ENSMUSG00000017721 .
GeneIDi 78928.
KEGGi mmu:78928.
UCSCi uc008nvc.1. mouse.

Organism-specific databases

CTDi 51604.
MGIi MGI:1926178. Pigt.

Phylogenomic databases

eggNOGi NOG331717.
GeneTreei ENSGT00390000018558.
HOGENOMi HOG000215945.
HOVERGENi HBG049395.
InParanoidi A2A5G2.
KOi K05292.
OMAi TLEVNPP.
OrthoDBi EOG70PBXH.
PhylomeDBi Q8BXQ2.
TreeFami TF105921.

Enzyme and pathway databases

UniPathwayi UPA00196 .

Miscellaneous databases

NextBioi 349782.
PROi Q8BXQ2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BXQ2.
Bgeei Q8BXQ2.
Genevestigatori Q8BXQ2.

Family and domain databases

InterProi IPR007245. PIG-T.
[Graphical view ]
PANTHERi PTHR12959. PTHR12959. 1 hit.
Pfami PF04113. Gpi16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Cloning and identification of differentially expressed transcripts in primary culture of GABAergic neurons."
    Li Z., Li Q., Sun C.X., Hertz L., Yu A.C.H.
    Neurochem. Res. 26:1101-1105(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-582.
    Strain: ICR.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-582.
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168.

Entry informationi

Entry nameiPIGT_MOUSE
AccessioniPrimary (citable) accession number: Q8BXQ2
Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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