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Protein

GPI transamidase component PIG-T

Gene

Pigt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).By similarity

Pathwayi

GO - Biological processi

  1. attachment of GPI anchor to protein Source: UniProtKB
  2. neuron apoptotic process Source: MGI
  3. neuron differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiREACT_273996. Attachment of GPI anchor to uPAR.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI transamidase component PIG-T
Alternative name(s):
Neuronal development-associated protein 7
Phosphatidylinositol-glycan biosynthesis class T protein
Gene namesi
Name:Pigt
Synonyms:Ndap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926178. Pigt.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 525500LumenalSequence AnalysisAdd
BLAST
Transmembranei526 – 54823HelicalSequence AnalysisAdd
BLAST
Topological domaini549 – 58234CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoplasmic membrane-bounded vesicle Source: MGI
  3. GPI-anchor transamidase complex Source: UniProtKB
  4. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 582557GPI transamidase component PIG-TPRO_0000024108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication
Disulfide bondi186 – 186Interchain (with C-92 in PIGK/GPI8)By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BXQ2.
PaxDbiQ8BXQ2.
PRIDEiQ8BXQ2.

PTM databases

PhosphoSiteiQ8BXQ2.

Expressioni

Gene expression databases

BgeeiQ8BXQ2.
ExpressionAtlasiQ8BXQ2. baseline and differential.
GenevestigatoriQ8BXQ2.

Interactioni

Subunit structurei

Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BXQ2. 1 interaction.
MINTiMINT-4107578.

Structurei

3D structure databases

ProteinModelPortaliQ8BXQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGT family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG331717.
GeneTreeiENSGT00390000018558.
HOGENOMiHOG000215945.
HOVERGENiHBG049395.
InParanoidiQ8BXQ2.
KOiK05292.
OMAiTLEVNPP.
OrthoDBiEOG70PBXH.
PhylomeDBiQ8BXQ2.
TreeFamiTF105921.

Family and domain databases

InterProiIPR007245. PIG-T.
[Graphical view]
PANTHERiPTHR12959. PTHR12959. 1 hit.
PfamiPF04113. Gpi16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BXQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA
60 70 80 90 100
TFQFRTRWDS DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT
110 120 130 140 150
RYWGPPFLQA PSGAELWVWF QDTVTDVDKS WRELSNVLSG IFCASLNFID
160 170 180 190 200
ATNTVTPTAS FKPLGLANDT DDYFLRYAVL PREVVCTENL TPWKKLLPCS
210 220 230 240 250
SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW ELRQTLSVVF
260 270 280 290 300
DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV
310 320 330 340 350
SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA
360 370 380 390 400
LPVPFLHAQR YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY
410 420 430 440 450
VHTLTITSKG KENKPSYIHY QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF
460 470 480 490 500
ERALLKWTEY TPDPNHGFYV SPSVLSALVP SVVAAKPVDW EGSPLFNTLF
510 520 530 540 550
PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV AVCYGSFYNL
560 570 580
LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL
Length:582
Mass (Da):65,705
Last modified:February 29, 2004 - v2
Checksum:i39FF3CF98D3F9322
GO

Sequence cautioni

The sequence AAH34175.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAK32809.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB39711.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAC34729.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti346 – 3461P → Q in BAC31951 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044499 mRNA. Translation: BAC31951.1.
AK155057 mRNA. Translation: BAE33018.1.
AL591478 Genomic DNA. Translation: CAM15012.2.
AJ308886 mRNA. Translation: CAC34729.1. Different initiation.
AF361435 mRNA. Translation: AAK32809.1. Different initiation.
AB057593 mRNA. Translation: BAB39711.1. Different initiation.
BC034175 mRNA. Translation: AAH34175.1. Different initiation.
CCDSiCCDS17038.2.
RefSeqiNP_598540.2. NM_133779.2.
XP_006500484.2. XM_006500421.2.
UniGeneiMm.28228.

Genome annotation databases

EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
GeneIDi78928.
KEGGimmu:78928.
UCSCiuc008nvc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044499 mRNA. Translation: BAC31951.1.
AK155057 mRNA. Translation: BAE33018.1.
AL591478 Genomic DNA. Translation: CAM15012.2.
AJ308886 mRNA. Translation: CAC34729.1. Different initiation.
AF361435 mRNA. Translation: AAK32809.1. Different initiation.
AB057593 mRNA. Translation: BAB39711.1. Different initiation.
BC034175 mRNA. Translation: AAH34175.1. Different initiation.
CCDSiCCDS17038.2.
RefSeqiNP_598540.2. NM_133779.2.
XP_006500484.2. XM_006500421.2.
UniGeneiMm.28228.

3D structure databases

ProteinModelPortaliQ8BXQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BXQ2. 1 interaction.
MINTiMINT-4107578.

PTM databases

PhosphoSiteiQ8BXQ2.

Proteomic databases

MaxQBiQ8BXQ2.
PaxDbiQ8BXQ2.
PRIDEiQ8BXQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
GeneIDi78928.
KEGGimmu:78928.
UCSCiuc008nvc.1. mouse.

Organism-specific databases

CTDi51604.
MGIiMGI:1926178. Pigt.

Phylogenomic databases

eggNOGiNOG331717.
GeneTreeiENSGT00390000018558.
HOGENOMiHOG000215945.
HOVERGENiHBG049395.
InParanoidiQ8BXQ2.
KOiK05292.
OMAiTLEVNPP.
OrthoDBiEOG70PBXH.
PhylomeDBiQ8BXQ2.
TreeFamiTF105921.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiREACT_273996. Attachment of GPI anchor to uPAR.

Miscellaneous databases

NextBioi349782.
PROiQ8BXQ2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BXQ2.
ExpressionAtlasiQ8BXQ2. baseline and differential.
GenevestigatoriQ8BXQ2.

Family and domain databases

InterProiIPR007245. PIG-T.
[Graphical view]
PANTHERiPTHR12959. PTHR12959. 1 hit.
PfamiPF04113. Gpi16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Cloning and identification of differentially expressed transcripts in primary culture of GABAergic neurons."
    Li Z., Li Q., Sun C.X., Hertz L., Yu A.C.H.
    Neurochem. Res. 26:1101-1105(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-582.
    Strain: ICR.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-582.
    Tissue: Mammary tumor.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168.

Entry informationi

Entry nameiPIGT_MOUSE
AccessioniPrimary (citable) accession number: Q8BXQ2
Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 2004
Last sequence update: February 29, 2004
Last modified: March 31, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.