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Q8BXQ2

- PIGT_MOUSE

UniProt

Q8BXQ2 - PIGT_MOUSE

Protein

GPI transamidase component PIG-T

Gene

Pigt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates By similarity.By similarity

    Pathwayi

    GO - Biological processi

    1. attachment of GPI anchor to protein Source: UniProtKB
    2. neuron apoptotic process Source: MGI
    3. neuron differentiation Source: MGI

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00196.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI transamidase component PIG-T
    Alternative name(s):
    Neuronal development-associated protein 7
    Phosphatidylinositol-glycan biosynthesis class T protein
    Gene namesi
    Name:Pigt
    Synonyms:Ndap7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1926178. Pigt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytoplasmic membrane-bounded vesicle Source: MGI
    3. GPI-anchor transamidase complex Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 582557GPI transamidase component PIG-TPRO_0000024108Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication
    Disulfide bondi186 – 186Interchain (with C-92 in PIGK/GPI8)By similarity
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ8BXQ2.
    PaxDbiQ8BXQ2.
    PRIDEiQ8BXQ2.

    PTM databases

    PhosphoSiteiQ8BXQ2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BXQ2.
    BgeeiQ8BXQ2.
    GenevestigatoriQ8BXQ2.

    Interactioni

    Subunit structurei

    Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BXQ2. 1 interaction.
    MINTiMINT-4107578.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BXQ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 525500LumenalSequence AnalysisAdd
    BLAST
    Topological domaini549 – 58234CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei526 – 54823HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PIGT family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG331717.
    GeneTreeiENSGT00390000018558.
    HOGENOMiHOG000215945.
    HOVERGENiHBG049395.
    InParanoidiA2A5G2.
    KOiK05292.
    OMAiTLEVNPP.
    OrthoDBiEOG70PBXH.
    PhylomeDBiQ8BXQ2.
    TreeFamiTF105921.

    Family and domain databases

    InterProiIPR007245. PIG-T.
    [Graphical view]
    PANTHERiPTHR12959. PTHR12959. 1 hit.
    PfamiPF04113. Gpi16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BXQ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA    50
    TFQFRTRWDS DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT 100
    RYWGPPFLQA PSGAELWVWF QDTVTDVDKS WRELSNVLSG IFCASLNFID 150
    ATNTVTPTAS FKPLGLANDT DDYFLRYAVL PREVVCTENL TPWKKLLPCS 200
    SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW ELRQTLSVVF 250
    DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV 300
    SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA 350
    LPVPFLHAQR YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY 400
    VHTLTITSKG KENKPSYIHY QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF 450
    ERALLKWTEY TPDPNHGFYV SPSVLSALVP SVVAAKPVDW EGSPLFNTLF 500
    PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV AVCYGSFYNL 550
    LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL 582
    Length:582
    Mass (Da):65,705
    Last modified:March 1, 2004 - v2
    Checksum:i39FF3CF98D3F9322
    GO

    Sequence cautioni

    The sequence AAH34175.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAK32809.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB39711.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAC34729.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti346 – 3461P → Q in BAC31951. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK044499 mRNA. Translation: BAC31951.1.
    AK155057 mRNA. Translation: BAE33018.1.
    AL591478 Genomic DNA. Translation: CAM15012.2.
    AJ308886 mRNA. Translation: CAC34729.1. Different initiation.
    AF361435 mRNA. Translation: AAK32809.1. Different initiation.
    AB057593 mRNA. Translation: BAB39711.1. Different initiation.
    BC034175 mRNA. Translation: AAH34175.1. Different initiation.
    CCDSiCCDS17038.2.
    RefSeqiNP_598540.2. NM_133779.2.
    UniGeneiMm.28228.

    Genome annotation databases

    EnsembliENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
    GeneIDi78928.
    KEGGimmu:78928.
    UCSCiuc008nvc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK044499 mRNA. Translation: BAC31951.1 .
    AK155057 mRNA. Translation: BAE33018.1 .
    AL591478 Genomic DNA. Translation: CAM15012.2 .
    AJ308886 mRNA. Translation: CAC34729.1 . Different initiation.
    AF361435 mRNA. Translation: AAK32809.1 . Different initiation.
    AB057593 mRNA. Translation: BAB39711.1 . Different initiation.
    BC034175 mRNA. Translation: AAH34175.1 . Different initiation.
    CCDSi CCDS17038.2.
    RefSeqi NP_598540.2. NM_133779.2.
    UniGenei Mm.28228.

    3D structure databases

    ProteinModelPortali Q8BXQ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BXQ2. 1 interaction.
    MINTi MINT-4107578.

    PTM databases

    PhosphoSitei Q8BXQ2.

    Proteomic databases

    MaxQBi Q8BXQ2.
    PaxDbi Q8BXQ2.
    PRIDEi Q8BXQ2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103101 ; ENSMUSP00000099390 ; ENSMUSG00000017721 .
    GeneIDi 78928.
    KEGGi mmu:78928.
    UCSCi uc008nvc.1. mouse.

    Organism-specific databases

    CTDi 51604.
    MGIi MGI:1926178. Pigt.

    Phylogenomic databases

    eggNOGi NOG331717.
    GeneTreei ENSGT00390000018558.
    HOGENOMi HOG000215945.
    HOVERGENi HBG049395.
    InParanoidi A2A5G2.
    KOi K05292.
    OMAi TLEVNPP.
    OrthoDBi EOG70PBXH.
    PhylomeDBi Q8BXQ2.
    TreeFami TF105921.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .

    Miscellaneous databases

    NextBioi 349782.
    PROi Q8BXQ2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BXQ2.
    Bgeei Q8BXQ2.
    Genevestigatori Q8BXQ2.

    Family and domain databases

    InterProi IPR007245. PIG-T.
    [Graphical view ]
    PANTHERi PTHR12959. PTHR12959. 1 hit.
    Pfami PF04113. Gpi16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Retina.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Cloning and identification of differentially expressed transcripts in primary culture of GABAergic neurons."
      Li Z., Li Q., Sun C.X., Hertz L., Yu A.C.H.
      Neurochem. Res. 26:1101-1105(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-582.
      Strain: ICR.
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-582.
      Tissue: Mammary tumor.
    5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168.

    Entry informationi

    Entry nameiPIGT_MOUSE
    AccessioniPrimary (citable) accession number: Q8BXQ2
    Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3