GPI transamidase component PIG-T precursor

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Q8BXQ2 (PIGT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GPI transamidase component PIG-T

Alternative name(s):
Neuronal development-associated protein 7
Phosphatidylinositol-glycan biosynthesis class T protein

Gene names

Name:	Pigt
Synonyms:	Ndap7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Forms a complex with PIGK/GPI8, PIGS, PIGU and GPAA1/GAA1. Has a critical role in maintaining the complex by stabilizing the expression of GPAA1 and GPI8 and linking them to PIGS By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Post-translational modification	The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity By similarity.
Sequence similarities	Belongs to the PIGT family.
Sequence caution	The sequence AAH34175.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK32809.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB39711.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC34729.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	attachment of GPI anchor to protein Traceable author statement PubMed 11483512. Source: UniProtKB neuron apoptotic process Inferred from direct assay PubMed 16516892. Source: MGI neuron differentiation Inferred from direct assay PubMed 16516892. Source: MGI
Cellular_component	GPI-anchor transamidase complex Inferred from direct assay PubMed 11483512. Source: UniProtKB cytoplasmic membrane-bounded vesicle Inferred from direct assay PubMed 16516892. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

Potential

Chain

26 – 582

557

GPI transamidase component PIG-T

PRO_0000024108

Regions

Topological domain

26 – 525

500

Lumenal Potential

Transmembrane

526 – 548

Helical; Potential

Topological domain

549 – 582

Cytoplasmic Potential

Amino acid modifications

Glycosylation

168

N-linked (GlcNAc...) Ref.5

Glycosylation

295

N-linked (GlcNAc...) Potential

Glycosylation

331

N-linked (GlcNAc...) Potential

Disulfide bond

186

Interchain (with C-92 in PIGK/GPI8) By similarity

Experimental info

Sequence conflict

346

P → Q in BAC31951. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8BXQ2 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 39FF3CF98D3F9322
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FASTA

582

65,705

        10         20         30         40         50         60 
MAAAMPLGLP LRLLVLLLVG RGCCGCAEGP RDSLREELVI TPLPSGDVAA TFQFRTRWDS 

        70         80         90        100        110        120 
DLQREGVSHY RLFPKALGQL ISKYSLRELH LSFTQGFWRT RYWGPPFLQA PSGAELWVWF 

       130        140        150        160        170        180 
QDTVTDVDKS WRELSNVLSG IFCASLNFID ATNTVTPTAS FKPLGLANDT DDYFLRYAVL 

       190        200        210        220        230        240 
PREVVCTENL TPWKKLLPCS SKAGLSVLLK ADRLFHTSYH SQAVHIRPIC RNAHCTSISW 

       250        260        270        280        290        300 
ELRQTLSVVF DAFITGQGKK DWSLFRMFSR TLTEACPLAS QSLVYVDITG YSQDNETLEV 

       310        320        330        340        350        360 
SPPPTSTYQD VILGTRKTYA VYDLFDTAMI NNSRNLNIQL KWKRPPDNEA LPVPFLHAQR 

       370        380        390        400        410        420 
YVSGYGLQKG ELSTLLYNSH PYRAFPVLLL DVVPWYLRLY VHTLTITSKG KENKPSYIHY 

       430        440        450        460        470        480 
QPAQDRQQPH LLEMLIQLPA NSVTKVSIQF ERALLKWTEY TPDPNHGFYV SPSVLSALVP 

       490        500        510        520        530        540 
SVVAAKPVDW EGSPLFNTLF PVSDGSSYFV RLYTEPLLVN LPTPDFSMPY NVICLTCTVV 

       550        560        570        580 
AVCYGSFYNL LTRTFHIEEP KSGGLAKRLA NLIRRARGVP PL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Retina.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Cloning and identification of differentially expressed transcripts in primary culture of GABAergic neurons." Li Z., Li Q., Sun C.X., Hertz L., Yu A.C.H. Neurochem. Res. 26:1101-1105(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-582. Strain: ICR. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-582. Tissue: Mammary tumor.
[5]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK044499 mRNA. Translation: BAC31951.1. AK155057 mRNA. Translation: BAE33018.1. AL591478 Genomic DNA. Translation: CAM15012.2. AJ308886 mRNA. Translation: CAC34729.1. Different initiation. AF361435 mRNA. Translation: AAK32809.1. Different initiation. AB057593 mRNA. Translation: BAB39711.1. Different initiation. BC034175 mRNA. Translation: AAH34175.1. Different initiation.
IPI	IPI00226790.
RefSeq	NP_598540.2. NM_133779.2.
UniGene	Mm.28228.
3D structure databases
ProteinModelPortal	Q8BXQ2.
ModBase	Search...
PTM databases
PhosphoSite	Q8BXQ2.
Proteomic databases
PaxDb	Q8BXQ2.
PRIDE	Q8BXQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103101; ENSMUSP00000099390; ENSMUSG00000017721.
GeneID	78928.
KEGG	mmu:78928.
UCSC	uc008nvc.1. mouse.
Organism-specific databases
CTD	51604.
MGI	MGI:1926178. Pigt.
Phylogenomic databases
eggNOG	NOG331717.
GeneTree	ENSGT00390000018558.
HOGENOM	HOG000215945.
HOVERGEN	HBG049395.
InParanoid	A2A5G2.
KO	K05292.
OMA	LQLKWKR.
OrthoDB	EOG4R7V9J.
Enzyme and pathway databases
UniPathway	UPA00196.
Gene expression databases
ArrayExpress	Q8BXQ2.
Bgee	Q8BXQ2.
Genevestigator	Q8BXQ2.
GermOnline	ENSMUSG00000017721. Mus musculus.
Family and domain databases
InterPro	IPR007245. PIG-T. [Graphical view]
PANTHER	PTHR12959. PTHR12959. 1 hit.
Pfam	PF04113. Gpi16. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	349782.
SOURCE	Search...

Entry information

Entry name

PIGT_MOUSE

Accession

Primary (citable) accession number: Q8BXQ2
Secondary accession number(s): A2A5G2, Q3U2X2, Q99JA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents