Q8BXK9 (CLIC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloride intracellular channel protein 5

Gene names

Name:

Clic5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Ref.3
Subunit structure	Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1. Interacts with AKAP9 By similarity.
Subcellular location	Golgi apparatus By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Note: Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity.
Tissue specificity	Detected in lung and inner ear. Detected in embryonic cochlea, on microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and on stereocilia of inner and outer hair cells (at protein level). Ref.3
Domain	Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.
Involvement in disease	Defects in Clic5 are a cause of the jitterbug (jbg) phenotype. Jbg is the result of a spontaneous mutation that leads to severe degeneration of the organ of Corti in the inner ear. Jbg leads to progressive degeneration of inner ear hair cells. Affected mice are identified by head bobbing, circling behavior and their inability to swim. They cannot hear well when young, and become completely deaf after 5 months. Ref.3
Sequence similarities	Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	auditory receptor cell stereocilium organization Inferred from mutant phenotype Ref.3. Source: MGI diet induced thermogenesis Inferred from mutant phenotype PubMed 20357015. Source: MGI neuromuscular process controlling balance Inferred from mutant phenotype Ref.3. Source: MGI protein localization Inferred from mutant phenotype Ref.3. Source: MGI sensory perception of sound Inferred from mutant phenotype Ref.3. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell actin cytoskeleton Inferred from sequence or structural similarity. Source: UniProtKB chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell stereocilium Inferred from direct assay Ref.3. Source: MGI
Molecular_function	voltage-gated chloride channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Chloride intracellular channel protein 5		PRO_0000144215
Regions
	Transmembrane	34 – 54	21	Helical; Note=After insertion into the membrane; Potential
	Domain	101 – 241	141	GST C-terminal
	Region	1 – 98	98	Required for insertion into the membrane By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8BXK9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7055A17D22977974	FASTA	251	28,287
	10 20 30 40 50 60 MTDSATTNGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN VTTVDLKRKP 70 80 90 100 110 120 ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK YPKLAAKHRE SNTAGIDIFS 130 140 150 160 170 180 KFSAYIKNTK QQNNAALERG LTKALRKLDD YLNSPLPEEI DTNTHGDEKG SQRKFLDGDE 190 200 210 220 230 240 LTLADCNLLP KLHVVKIVAK KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA 250 YADVARRLSR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Adrenal gland and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[3]	"The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function." Gagnon L.H., Longo-Guess C.M., Berryman M., Shin J.-B., Saylor K.W., Yu H., Gillespie P.G., Johnson K.R. J. Neurosci. 26:10188-10198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISEASE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK046522 mRNA. Translation: BAC32769.1. AK156849 mRNA. Translation: BAE33875.1. BC064037 mRNA. Translation: AAH64037.1.
IPI	IPI00226730.
RefSeq	NP_766209.1. NM_172621.2.
UniGene	Mm.37666.
3D structure databases
ProteinModelPortal	Q8BXK9.
SMR	Q8BXK9. Positions 10-250.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8BXK9. 1 interaction.
PTM databases
PhosphoSite	Q8BXK9.
Proteomic databases
PaxDb	Q8BXK9.
PRIDE	Q8BXK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959.
GeneID	224796.
KEGG	mmu:224796.
UCSC	uc008cpw.1. mouse.
Organism-specific databases
CTD	53405.
MGI	MGI:1917912. Clic5.
Phylogenomic databases
eggNOG	NOG282171.
GeneTree	ENSGT00550000074477.
HOGENOM	HOG000231548.
HOVERGEN	HBG050994.
InParanoid	Q8BXK9.
KO	K05025.
OMA	NGDDRDP.
OrthoDB	EOG40S0FN.
Gene expression databases
Bgee	Q8BXK9.
CleanEx	MM_CLIC5.
Genevestigator	Q8BXK9.
GermOnline	ENSMUSG00000023959. Mus musculus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR002946. Int_Cl_channel. IPR012336. Thioredoxin-like_fold. [Graphical view]
PRINTS	PR01263. INTCLCHANNEL.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR00862. O-ClC. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CLIC5. mouse.
NextBio	377369.
SOURCE	Search...

Entry information

Entry name

CLIC5_MOUSE

Accession

Primary (citable) accession number: Q8BXK9
Secondary accession number(s): Q3U0H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program