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Q8BXK9

- CLIC5_MOUSE

UniProt

Q8BXK9 - CLIC5_MOUSE

Protein

Chloride intracellular channel protein 5

Gene

Clic5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti.1 Publication

    GO - Molecular functioni

    1. voltage-gated chloride channel activity Source: InterPro

    GO - Biological processi

    1. auditory receptor cell stereocilium organization Source: MGI
    2. diet induced thermogenesis Source: MGI
    3. neuromuscular process controlling balance Source: MGI
    4. protein localization Source: MGI
    5. response to dietary excess Source: MGI
    6. sensory perception of sound Source: MGI

    Keywords - Molecular functioni

    Chloride channel, Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloride intracellular channel protein 5
    Gene namesi
    Name:Clic5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1917912. Clic5.

    Subcellular locationi

    Golgi apparatus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Membrane By similarity; Single-pass membrane protein By similarity. Cytoplasm By similarity
    Note: Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: UniProtKB
    2. chloride channel complex Source: UniProtKB-KW
    3. Golgi apparatus Source: UniProtKB-SubCell
    4. microtubule organizing center Source: UniProtKB-SubCell
    5. stereocilium Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Clic5 are a cause of the jitterbug (jbg) phenotype. Jbg is the result of a spontaneous mutation that leads to severe degeneration of the organ of Corti in the inner ear. Jbg leads to progressive degeneration of inner ear hair cells. Affected mice are identified by head bobbing, circling behavior and their inability to swim. They cannot hear well when young, and become completely deaf after 5 months.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Chloride intracellular channel protein 5PRO_0000144215Add
    BLAST

    Proteomic databases

    MaxQBiQ8BXK9.
    PaxDbiQ8BXK9.
    PRIDEiQ8BXK9.

    PTM databases

    PhosphoSiteiQ8BXK9.

    Expressioni

    Tissue specificityi

    Detected in lung and inner ear. Detected in embryonic cochlea, on microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and on stereocilia of inner and outer hair cells (at protein level).1 Publication

    Gene expression databases

    BgeeiQ8BXK9.
    CleanExiMM_CLIC5.
    GenevestigatoriQ8BXK9.

    Interactioni

    Subunit structurei

    Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1. Interacts with AKAP9 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BXK9. 4 interactions.
    MINTiMINT-4091024.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BXK9.
    SMRiQ8BXK9. Positions 10-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 5421Helical; Note=After insertion into the membraneSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 241141GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9898Required for insertion into the membraneBy similarityAdd
    BLAST

    Domaini

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.By similarity

    Sequence similaritiesi

    Belongs to the chloride channel CLIC family.Curated
    Contains 1 GST C-terminal domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG282171.
    GeneTreeiENSGT00550000074477.
    HOGENOMiHOG000231548.
    HOVERGENiHBG050994.
    InParanoidiQ8BXK9.
    KOiK05025.
    OMAiGAICKAG.
    OrthoDBiEOG7X3QR3.
    PhylomeDBiQ8BXK9.
    TreeFamiTF315438.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR002946. Int_Cl_channel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF13417. GST_N_3. 1 hit.
    [Graphical view]
    PRINTSiPR01263. INTCLCHANNEL.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR00862. O-ClC. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BXK9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDSATTNGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN    50
    VTTVDLKRKP ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK 100
    YPKLAAKHRE SNTAGIDIFS KFSAYIKNTK QQNNAALERG LTKALRKLDD 150
    YLNSPLPEEI DTNTHGDEKG SQRKFLDGDE LTLADCNLLP KLHVVKIVAK 200
    KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA YADVARRLSR 250
    S 251
    Length:251
    Mass (Da):28,287
    Last modified:March 1, 2003 - v1
    Checksum:i7055A17D22977974
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046522 mRNA. Translation: BAC32769.1.
    AK156849 mRNA. Translation: BAE33875.1.
    BC064037 mRNA. Translation: AAH64037.1.
    CCDSiCCDS28804.1.
    RefSeqiNP_766209.1. NM_172621.2.
    UniGeneiMm.37666.

    Genome annotation databases

    EnsembliENSMUST00000024755; ENSMUSP00000024755; ENSMUSG00000023959.
    GeneIDi224796.
    KEGGimmu:224796.
    UCSCiuc008cpw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046522 mRNA. Translation: BAC32769.1 .
    AK156849 mRNA. Translation: BAE33875.1 .
    BC064037 mRNA. Translation: AAH64037.1 .
    CCDSi CCDS28804.1.
    RefSeqi NP_766209.1. NM_172621.2.
    UniGenei Mm.37666.

    3D structure databases

    ProteinModelPortali Q8BXK9.
    SMRi Q8BXK9. Positions 10-250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BXK9. 4 interactions.
    MINTi MINT-4091024.

    PTM databases

    PhosphoSitei Q8BXK9.

    Proteomic databases

    MaxQBi Q8BXK9.
    PaxDbi Q8BXK9.
    PRIDEi Q8BXK9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024755 ; ENSMUSP00000024755 ; ENSMUSG00000023959 .
    GeneIDi 224796.
    KEGGi mmu:224796.
    UCSCi uc008cpw.1. mouse.

    Organism-specific databases

    CTDi 53405.
    MGIi MGI:1917912. Clic5.

    Phylogenomic databases

    eggNOGi NOG282171.
    GeneTreei ENSGT00550000074477.
    HOGENOMi HOG000231548.
    HOVERGENi HBG050994.
    InParanoidi Q8BXK9.
    KOi K05025.
    OMAi GAICKAG.
    OrthoDBi EOG7X3QR3.
    PhylomeDBi Q8BXK9.
    TreeFami TF315438.

    Miscellaneous databases

    ChiTaRSi CLIC5. mouse.
    NextBioi 377369.
    PROi Q8BXK9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BXK9.
    CleanExi MM_CLIC5.
    Genevestigatori Q8BXK9.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR002946. Int_Cl_channel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    PRINTSi PR01263. INTCLCHANNEL.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR00862. O-ClC. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Adrenal gland and Spleen.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. "The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function."
      Gagnon L.H., Longo-Guess C.M., Berryman M., Shin J.-B., Saylor K.W., Yu H., Gillespie P.G., Johnson K.R.
      J. Neurosci. 26:10188-10198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISEASE.

    Entry informationi

    Entry nameiCLIC5_MOUSE
    AccessioniPrimary (citable) accession number: Q8BXK9
    Secondary accession number(s): Q3U0H8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3