Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8BXK8

- AGAP1_MOUSE

UniProt

Q8BXK8 - AGAP1_MOUSE

Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1

Gene

Agap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system By similarity.By similarity

    Enzyme regulationi

    GAP activity stimulated by phosphatidylinositol 3,4,5-trisphosphate (PIP3) and, to a lesser extent, by phosphatidylinositol 4,5-bisphosphate (PIP2). Phosphatidic acid potentiates PIP2 stimulation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 858GTPSequence Analysis
    Nucleotide bindingi122 – 1265GTPSequence Analysis
    Nucleotide bindingi178 – 1814GTPSequence Analysis
    Zinc fingeri624 – 64724C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. GTPase activity Source: MGI
    3. GTP binding Source: UniProtKB-KW
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. protein transport Source: UniProtKB-KW
    3. regulation of ARF GTPase activity Source: InterPro
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
    Short name:
    AGAP-1
    Alternative name(s):
    Centaurin-gamma-2
    Short name:
    Cnt-g2
    Gene namesi
    Name:Agap1
    Synonyms:Centg2, Kiaa1099
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2653690. Agap1.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Associates with the endocytic compartment.By similarity

    GO - Cellular componenti

    1. cytosol Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 857857Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1PRO_0000074219Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei836 – 8361PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosines.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BXK8.
    PRIDEiQ8BXK8.

    PTM databases

    PhosphoSiteiQ8BXK8.

    Expressioni

    Tissue specificityi

    Widely expressed, with highest levels in brain and kidney.1 Publication

    Developmental stagei

    At 12.5 dpc, expression is restricted to neural tube, forebrain and midbrain.1 Publication

    Gene expression databases

    ArrayExpressiQ8BXK8.
    BgeeiQ8BXK8.
    GenevestigatoriQ8BXK8.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with several subunits of the AP-3 protein complex: AP3M1, AP3S1 and AP3S2. Interacts with GUCY1A3 and GUCY1B3 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BXK8.
    SMRiQ8BXK8. Positions 70-233, 344-422, 530-592, 615-854.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini346 – 588243PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini609 – 729121Arf-GAPPROSITE-ProRule annotationAdd
    BLAST
    Repeati768 – 79730ANK 1Add
    BLAST
    Repeati801 – 83030ANK 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni66 – 276211Small GTPase-likeAdd
    BLAST

    Domaini

    The PH domain mediates AP-3 binding.

    Sequence similaritiesi

    Belongs to the centaurin gamma-like family.Curated
    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri624 – 64724C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    GeneTreeiENSGT00750000117646.
    HOGENOMiHOG000007233.
    HOVERGENiHBG054045.
    InParanoidiQ8BXK8.
    KOiK12491.
    OMAiDGMQQRS.
    OrthoDBiEOG72VH5V.
    PhylomeDBiQ8BXK8.
    TreeFamiTF317762.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    2.30.29.30. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF08477. Miro. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00248. ANK. 2 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BXK8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYQQQLANS AAIRAEIQRF ESVHPNIYSI YELLERVEEP VLQNQIREHV    50
    IAIEDAFVNS QEWTLSRSVP ELKVGIVGNL ASGKSALVHR YLTGTYVQEE 100
    SPEGGRFKKE IVVDGQSYLL LIRDEGGPPE AQFAMWVDAV IFVFSLEDEI 150
    SFQTVYHYYS RMANYRNTSE IPLVLVGTQD AISSTNPRVI DDVRARKLSN 200
    DLKRCTYYET CATYGLNVER VFQDVAQKIV ATRKKQQLSI GPCKSLPNSP 250
    SHSSVCSAQV SAVHISQTSN GGGSLSDYSS SVPSTPSTSQ KELRIDVPPT 300
    ANTPTPVRKQ SKRRSNLFTS RKGSDPDKEK KGLESRADSI GSGRAIPIKQ 350
    GMLLKRSGKS LNKEWKKKYV TLCDNGVLTY HPSLHDYMQN VHGKEIDLLR 400
    TTVKVPGKRP PRATSACAPI SSPKTNGLAK DMSSLHISPN SGNVTSASGS 450
    QMASGISLVS FNSRPDGMHQ RSYSVSSADQ WSDATVIANS AISSDTGLGD 500
    SVCSSPSISS STSPKLDPPP SPHANRKKHR RKKSTSNFKA DGLSGTAEEQ 550
    EENLEFIIVS LTGQTWHFEA TTYEERDAWV QAIESQILAS LQSCESSKNK 600
    SRLTSQSEAM ALQSIRNMRG NSHCVDCDTQ NPNWASLNLG ALMCIECSGI 650
    HRNLGTHLSR VRSLDLDDWP MELIKVMSSI GNELANSVWE EGSQGRTKPS 700
    LDSTREEKER WIRAKYEQKL FLAPLPCTEF SLGQQLLRAT AEEDLRTVIL 750
    LLAHGSRDEV NETCGEGDGR TALHLACRKG NVVLAQLLIW YGVDVMARDA 800
    HGNTALAYAR QASSQECIDV LLQYGCPDER FVLMATPNLS RKSNSRNNSS 850
    GRAPSVI 857
    Length:857
    Mass (Da):94,411
    Last modified:March 1, 2003 - v1
    Checksum:iA88AF73A4BB50815
    GO

    Sequence cautioni

    The sequence BAC98099.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti424 – 4241K → R in BAC98099. (PubMed:14621295)Curated
    Sequence conflicti668 – 6681D → Y in BAC29078. (PubMed:16141072)Curated
    Sequence conflicti700 – 7001S → P in BAE27957. (PubMed:16141072)Curated
    Sequence conflicti811 – 8111Q → H in BAC29078. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK035494 mRNA. Translation: BAC29078.1.
    AK046523 mRNA. Translation: BAC32770.1.
    AK147503 mRNA. Translation: BAE27957.1.
    AK129289 mRNA. Translation: BAC98099.2. Different initiation.
    CCDSiCCDS15149.1.
    RefSeqiNP_001032213.1. NM_001037136.1.
    NP_835220.1. NM_178119.3.
    UniGeneiMm.291135.

    Genome annotation databases

    EnsembliENSMUST00000027521; ENSMUSP00000027521; ENSMUSG00000055013.
    GeneIDi347722.
    KEGGimmu:347722.
    UCSCiuc007byw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK035494 mRNA. Translation: BAC29078.1 .
    AK046523 mRNA. Translation: BAC32770.1 .
    AK147503 mRNA. Translation: BAE27957.1 .
    AK129289 mRNA. Translation: BAC98099.2 . Different initiation.
    CCDSi CCDS15149.1.
    RefSeqi NP_001032213.1. NM_001037136.1.
    NP_835220.1. NM_178119.3.
    UniGenei Mm.291135.

    3D structure databases

    ProteinModelPortali Q8BXK8.
    SMRi Q8BXK8. Positions 70-233, 344-422, 530-592, 615-854.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BXK8.

    Proteomic databases

    PaxDbi Q8BXK8.
    PRIDEi Q8BXK8.

    Protocols and materials databases

    DNASUi 347722.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027521 ; ENSMUSP00000027521 ; ENSMUSG00000055013 .
    GeneIDi 347722.
    KEGGi mmu:347722.
    UCSCi uc007byw.1. mouse.

    Organism-specific databases

    CTDi 116987.
    MGIi MGI:2653690. Agap1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5347.
    GeneTreei ENSGT00750000117646.
    HOGENOMi HOG000007233.
    HOVERGENi HBG054045.
    InParanoidi Q8BXK8.
    KOi K12491.
    OMAi DGMQQRS.
    OrthoDBi EOG72VH5V.
    PhylomeDBi Q8BXK8.
    TreeFami TF317762.

    Miscellaneous databases

    ChiTaRSi AGAP1. mouse.
    NextBioi 400243.
    PROi Q8BXK8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BXK8.
    Bgeei Q8BXK8.
    Genevestigatori Q8BXK8.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    2.30.29.30. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF08477. Miro. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00248. ANK. 2 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Adrenal gland and Urinary bladder.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    3. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    4. "GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins."
      Xia C., Ma W., Stafford L.J., Liu C., Gong L., Martin J.F., Liu M.
      Mol. Cell. Biol. 23:2476-2488(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase."
      Meurer S., Pioch S., Wagner K., Mueller-Esterl W., Gross S.
      J. Biol. Chem. 279:49346-49354(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAGAP1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BXK8
    Secondary accession number(s): Q3UHA0, Q6ZPX9, Q8BZG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3