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Q8BXK8

- AGAP1_MOUSE

UniProt

Q8BXK8 - AGAP1_MOUSE

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Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1

Gene

Agap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system (By similarity).By similarity

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 3,4,5-trisphosphate (PIP3) and, to a lesser extent, by phosphatidylinositol 4,5-bisphosphate (PIP2). Phosphatidic acid potentiates PIP2 stimulation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 858GTPSequence Analysis
Nucleotide bindingi122 – 1265GTPSequence Analysis
Nucleotide bindingi178 – 1814GTPSequence Analysis
Zinc fingeri624 – 64724C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: InterPro
  2. GTPase activity Source: MGI
  3. GTP binding Source: UniProtKB-KW
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. protein transport Source: UniProtKB-KW
  3. regulation of ARF GTPase activity Source: InterPro
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
Short name:
AGAP-1
Alternative name(s):
Centaurin-gamma-2
Short name:
Cnt-g2
Gene namesi
Name:Agap1
Synonyms:Centg2, Kiaa1099
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2653690. Agap1.

Subcellular locationi

Cytoplasm By similarity
Note: Associates with the endocytic compartment.By similarity

GO - Cellular componenti

  1. cytosol Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1PRO_0000074219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei836 – 8361PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on tyrosines.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BXK8.
PaxDbiQ8BXK8.
PRIDEiQ8BXK8.

PTM databases

PhosphoSiteiQ8BXK8.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in brain and kidney.1 Publication

Developmental stagei

At 12.5 dpc, expression is restricted to neural tube, forebrain and midbrain.1 Publication

Gene expression databases

BgeeiQ8BXK8.
ExpressionAtlasiQ8BXK8. baseline and differential.
GenevestigatoriQ8BXK8.

Interactioni

Subunit structurei

Homodimer. Interacts with several subunits of the AP-3 protein complex: AP3M1, AP3S1 and AP3S2. Interacts with GUCY1A3 and GUCY1B3 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8BXK8.
SMRiQ8BXK8. Positions 70-233, 344-422, 530-592, 615-854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini346 – 588243PHPROSITE-ProRule annotationAdd
BLAST
Domaini609 – 729121Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Repeati768 – 79730ANK 1Add
BLAST
Repeati801 – 83030ANK 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni66 – 276211Small GTPase-likeAdd
BLAST

Domaini

The PH domain mediates AP-3 binding.

Sequence similaritiesi

Belongs to the centaurin gamma-like family.Curated
Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri624 – 64724C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000007233.
HOVERGENiHBG054045.
InParanoidiQ8BXK8.
KOiK12491.
OMAiDGMQQRS.
OrthoDBiEOG72VH5V.
PhylomeDBiQ8BXK8.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BXK8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYQQQLANS AAIRAEIQRF ESVHPNIYSI YELLERVEEP VLQNQIREHV
60 70 80 90 100
IAIEDAFVNS QEWTLSRSVP ELKVGIVGNL ASGKSALVHR YLTGTYVQEE
110 120 130 140 150
SPEGGRFKKE IVVDGQSYLL LIRDEGGPPE AQFAMWVDAV IFVFSLEDEI
160 170 180 190 200
SFQTVYHYYS RMANYRNTSE IPLVLVGTQD AISSTNPRVI DDVRARKLSN
210 220 230 240 250
DLKRCTYYET CATYGLNVER VFQDVAQKIV ATRKKQQLSI GPCKSLPNSP
260 270 280 290 300
SHSSVCSAQV SAVHISQTSN GGGSLSDYSS SVPSTPSTSQ KELRIDVPPT
310 320 330 340 350
ANTPTPVRKQ SKRRSNLFTS RKGSDPDKEK KGLESRADSI GSGRAIPIKQ
360 370 380 390 400
GMLLKRSGKS LNKEWKKKYV TLCDNGVLTY HPSLHDYMQN VHGKEIDLLR
410 420 430 440 450
TTVKVPGKRP PRATSACAPI SSPKTNGLAK DMSSLHISPN SGNVTSASGS
460 470 480 490 500
QMASGISLVS FNSRPDGMHQ RSYSVSSADQ WSDATVIANS AISSDTGLGD
510 520 530 540 550
SVCSSPSISS STSPKLDPPP SPHANRKKHR RKKSTSNFKA DGLSGTAEEQ
560 570 580 590 600
EENLEFIIVS LTGQTWHFEA TTYEERDAWV QAIESQILAS LQSCESSKNK
610 620 630 640 650
SRLTSQSEAM ALQSIRNMRG NSHCVDCDTQ NPNWASLNLG ALMCIECSGI
660 670 680 690 700
HRNLGTHLSR VRSLDLDDWP MELIKVMSSI GNELANSVWE EGSQGRTKPS
710 720 730 740 750
LDSTREEKER WIRAKYEQKL FLAPLPCTEF SLGQQLLRAT AEEDLRTVIL
760 770 780 790 800
LLAHGSRDEV NETCGEGDGR TALHLACRKG NVVLAQLLIW YGVDVMARDA
810 820 830 840 850
HGNTALAYAR QASSQECIDV LLQYGCPDER FVLMATPNLS RKSNSRNNSS

GRAPSVI
Length:857
Mass (Da):94,411
Last modified:March 1, 2003 - v1
Checksum:iA88AF73A4BB50815
GO

Sequence cautioni

The sequence BAC98099.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti424 – 4241K → R in BAC98099. (PubMed:14621295)Curated
Sequence conflicti668 – 6681D → Y in BAC29078. (PubMed:16141072)Curated
Sequence conflicti700 – 7001S → P in BAE27957. (PubMed:16141072)Curated
Sequence conflicti811 – 8111Q → H in BAC29078. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035494 mRNA. Translation: BAC29078.1.
AK046523 mRNA. Translation: BAC32770.1.
AK147503 mRNA. Translation: BAE27957.1.
AK129289 mRNA. Translation: BAC98099.2. Different initiation.
CCDSiCCDS15149.1.
RefSeqiNP_001032213.1. NM_001037136.1.
NP_835220.1. NM_178119.3.
UniGeneiMm.291135.

Genome annotation databases

EnsembliENSMUST00000027521; ENSMUSP00000027521; ENSMUSG00000055013.
GeneIDi347722.
KEGGimmu:347722.
UCSCiuc007byw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035494 mRNA. Translation: BAC29078.1 .
AK046523 mRNA. Translation: BAC32770.1 .
AK147503 mRNA. Translation: BAE27957.1 .
AK129289 mRNA. Translation: BAC98099.2 . Different initiation.
CCDSi CCDS15149.1.
RefSeqi NP_001032213.1. NM_001037136.1.
NP_835220.1. NM_178119.3.
UniGenei Mm.291135.

3D structure databases

ProteinModelPortali Q8BXK8.
SMRi Q8BXK8. Positions 70-233, 344-422, 530-592, 615-854.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8BXK8.

Proteomic databases

MaxQBi Q8BXK8.
PaxDbi Q8BXK8.
PRIDEi Q8BXK8.

Protocols and materials databases

DNASUi 347722.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027521 ; ENSMUSP00000027521 ; ENSMUSG00000055013 .
GeneIDi 347722.
KEGGi mmu:347722.
UCSCi uc007byw.1. mouse.

Organism-specific databases

CTDi 116987.
MGIi MGI:2653690. Agap1.
Rougei Search...

Phylogenomic databases

eggNOGi COG5347.
GeneTreei ENSGT00760000118874.
HOGENOMi HOG000007233.
HOVERGENi HBG054045.
InParanoidi Q8BXK8.
KOi K12491.
OMAi DGMQQRS.
OrthoDBi EOG72VH5V.
PhylomeDBi Q8BXK8.
TreeFami TF317762.

Miscellaneous databases

ChiTaRSi Agap1. mouse.
NextBioi 400243.
PROi Q8BXK8.
SOURCEi Search...

Gene expression databases

Bgeei Q8BXK8.
ExpressionAtlasi Q8BXK8. baseline and differential.
Genevestigatori Q8BXK8.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Adrenal gland and Urinary bladder.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins."
    Xia C., Ma W., Stafford L.J., Liu C., Gong L., Martin J.F., Liu M.
    Mol. Cell. Biol. 23:2476-2488(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase."
    Meurer S., Pioch S., Wagner K., Mueller-Esterl W., Gross S.
    J. Biol. Chem. 279:49346-49354(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAGAP1_MOUSE
AccessioniPrimary (citable) accession number: Q8BXK8
Secondary accession number(s): Q3UHA0, Q6ZPX9, Q8BZG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3