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Protein

Transcriptional-regulating factor 1

Gene

Trerf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri512 – 53423C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1019 – 104325C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1092 – 111423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional-regulating factor 1
Alternative name(s):
Transcriptional-regulating protein 132
Zinc finger transcription factor TReP-132
Gene namesi
Name:Trerf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2442086. Trerf1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12051205Transcriptional-regulating factor 1PRO_0000197135Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei490 – 4901PhosphoserineBy similarity
Modified residuei639 – 6391N6-acetyllysineCombined sources
Modified residuei646 – 6461N6-acetyllysineCombined sources
Modified residuei773 – 7731PhosphothreonineCombined sources
Modified residuei960 – 9601PhosphothreonineBy similarity
Modified residuei961 – 9611PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BXJ2.
PaxDbiQ8BXJ2.
PeptideAtlasiQ8BXJ2.
PRIDEiQ8BXJ2.

PTM databases

iPTMnetiQ8BXJ2.
PhosphoSiteiQ8BXJ2.

Expressioni

Tissue specificityi

Highly expressed in kidney, lung and brain. In the brain, expression was seen in the basal ganglia, hippocampus, piriform cortex, cerebral cortex, ventromedial nucleus of the hypothalamus and the dorsal and superior central nuclei of the raphe.1 Publication

Developmental stagei

At 15.5 dpc, highly expressed in brain, lung, adrenal, thymus and kidney. Expression was also seen in spinal cord, retina and snout.1 Publication

Gene expression databases

BgeeiENSMUSG00000064043.
CleanExiMM_TRERF1.

Interactioni

Subunit structurei

Interacts with CREBBP and EP300. Interacts with DNTTIP1 and DNTT.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230328. 1 interaction.
IntActiQ8BXJ2. 1 interaction.
STRINGi10090.ENSMUSP00000077103.

Structurei

3D structure databases

ProteinModelPortaliQ8BXJ2.
SMRiQ8BXJ2. Positions 1018-1044, 1091-1116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini785 – 87692ELM2PROSITE-ProRule annotationAdd
BLAST
Domaini891 – 94252SANTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi202 – 404203Gln-richAdd
BLAST
Compositional biasi552 – 755204Pro-richAdd
BLAST
Compositional biasi962 – 98726Glu-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 ELM2 domain.PROSITE-ProRule annotation
Contains 1 SANT domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri512 – 53423C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1019 – 104325C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1092 – 111423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4167. Eukaryota.
ENOG4110BKZ. LUCA.
HOGENOMiHOG000154675.
HOVERGENiHBG082398.
InParanoidiQ8BXJ2.
PhylomeDBiQ8BXJ2.
TreeFamiTF106431.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR000949. ELM2_dom.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01448. ELM2. 1 hit.
PF13912. zf-C2H2_6. 3 hits.
[Graphical view]
SMARTiSM01189. ELM2. 1 hit.
SM00717. SANT. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51156. ELM2. 1 hit.
PS51293. SANT. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BXJ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDQQLYKTN HVGHGGENLF YQQPPLGVHS GLGHSYGNTI SGAGMDAPQA
60 70 80 90 100
SPISPHFPQD TRDGLGLPIG SKNLGQMDTS RQGGWGSHAG PGNHVQLRSN
110 120 130 140 150
LANSNMMWGT PTQVEPADGY QYTYSQASEI RTQKLTSGVL HKLDSFTQVF
160 170 180 190 200
ANQNLRIQVN NMAQVLHTQS AVMDGASDSA LRQLLSQKPV EPSASAIASR
210 220 230 240 250
YQQVPQQPHP GFTGGLPKPA LPVGQHAPQG HLYYDYQQPL AQMSMQGGQP
260 270 280 290 300
LQAPQVLSGH MQQLQQHQYY PQPPPQQQQA GLQRISVQEM QQQQQPQQIR
310 320 330 340 350
PSPPQQQQQL QLQQRQSSLQ IPQYYQPQPM MQHLQEQQQP SMHLQPPSYH
360 370 380 390 400
RDPHQYTPEQ AHAVQLIQLG SMPQYYYQEP QQAYSHPLYP QSHLSQHQQR
410 420 430 440 450
EDGQLKTYSS DRQTPAMLSS HGDMGTSDTG VADPASSEMT RVTSTLPHQP
460 470 480 490 500
LLSPSGIHLN NMGSQHQQPP SPSAMWPQMH LPDGRAQSGS PESSSGQTKG
510 520 530 540 550
VFGEQFDAKN KLTCSICLKE FKSLPALNGH MRSHGGMRAS PSLKQEEGEK
560 570 580 590 600
APPPQPQPQP QPQQPLPPPP PPPPPPQLPP EAERLTPMVM PVSVPVKLIP
610 620 630 640 650
PKPSSQGFTN SVAATPAARD KPASSMSDDE MPVLEIPRKH PPIAAKVEEP
660 670 680 690 700
LKNLPEKKKF RHRPEPLFIP PPPSSYTPNP TSYSGATLYQ SQLRSPRILG
710 720 730 740 750
DHLLLDPAHE LPPYTPPPML SPVRQGSGLF SNVLISGHGP GVHPQLPLTP
760 770 780 790 800
LTPTPRVLLC RSSSIDGSNV TVTPGPGEQT VDVEPRINIG LRFQAEIPEL
810 820 830 840 850
QDVSALAQDT HRATLVWKPW PELENQALQQ QVENLLNLCC SSALPGGGTN
860 870 880 890 900
SEFALHSLFE AKGDVMATLE MLLLRKPVRL KCHPLANYHY AGSDKWTSLE
910 920 930 940 950
RKLFNKALAT YSKDFIFVQK MVKSKTVAQC VEYYYTWKKI MRLGRKHRTR
960 970 980 990 1000
LAEIIDDCMT SEEEEEAEEE EEDPEEDRKS IKEEESEVAK SPEPPPAPAL
1010 1020 1030 1040 1050
APTEGPPMQA VGQQPSGNFI CEMPNCGAVF SSRQALNGHA RIHGGTNQVA
1060 1070 1080 1090 1100
KTRGAIPSGK QKPGGTQSGY CSVKSSPSHS TTSGETDPTT IFPCKECGKV
1110 1120 1130 1140 1150
FFKIKSRNAH MKTHRQQEEQ QRQKAQKAAF AAEMAATIER TTGPVGAPEL
1160 1170 1180 1190 1200
LPLDQLSLMK PVKDVDILDD DVVQQLGVMD EAEVVGTDLL LDDQDSVLLQ

GDTEL
Length:1,205
Mass (Da):132,402
Last modified:March 1, 2003 - v1
Checksum:i322FCC31845A99E2
GO
Isoform 2 (identifier: Q8BXJ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     634-634: L → LVRMTLSPPHSPQGAAPRAPA

Show »
Length:1,225
Mass (Da):134,425
Checksum:i22F54805CD234944
GO
Isoform 3 (identifier: Q8BXJ2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     493-493: Missing.
     546-565: EEGEKAPPPQPQPQPQPQQP → VSREPAPSFPGHVLLALLCC
     566-1205: Missing.

Show »
Length:564
Mass (Da):62,165
Checksum:iC9DD7CCAE3D300C8
GO
Isoform 4 (identifier: Q8BXJ2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     493-493: Missing.
     1028-1028: A → ADCRCHVAPFLPQ

Show »
Length:1,216
Mass (Da):133,683
Checksum:iA2B68EE59AA97B1A
GO

Sequence cautioni

The sequence AAN02288 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891A → V in AAH59215 (PubMed:15489334).Curated
Sequence conflicti112 – 1121T → P in AAN02288 (PubMed:12556907).Curated
Sequence conflicti308 – 3092QQ → HR in AAN02288 (PubMed:12556907).Curated
Sequence conflicti368 – 3692QL → PA in AAN02288 (PubMed:12556907).Curated
Sequence conflicti464 – 4641S → T in AAN02288 (PubMed:12556907).Curated
Sequence conflicti659 – 6591K → E in AAN02288 (PubMed:12556907).Curated
Sequence conflicti915 – 9151F → L in AAN02288 (PubMed:12556907).Curated
Sequence conflicti1159 – 11591M → I in AAN02288 (PubMed:12556907).Curated
Sequence conflicti1202 – 12021D → V in AAN02288 (PubMed:12556907).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei493 – 4931Missing in isoform 3 and isoform 4. 2 PublicationsVSP_015647
Alternative sequencei546 – 56520EEGEK…QPQQP → VSREPAPSFPGHVLLALLCC in isoform 3. 1 PublicationVSP_015648Add
BLAST
Alternative sequencei566 – 1205640Missing in isoform 3. 1 PublicationVSP_015649Add
BLAST
Alternative sequencei634 – 6341L → LVRMTLSPPHSPQGAAPRAP A in isoform 2. 1 PublicationVSP_015650
Alternative sequencei1028 – 10281A → ADCRCHVAPFLPQ in isoform 4. 1 PublicationVSP_015651

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081774 mRNA. Translation: AAN02288.1. Frameshift.
AK042320 mRNA. Translation: BAC31222.1.
AK046822 mRNA. Translation: BAC32884.1.
BC051645 mRNA. Translation: AAH51645.1.
BC059215 mRNA. Translation: AAH59215.1.
BC108416 mRNA. Translation: AAI08417.1.
RefSeqiNP_001091092.1. NM_001097623.1. [Q8BXJ2-2]
NP_766210.1. NM_172622.2. [Q8BXJ2-1]
UniGeneiMm.260989.

Genome annotation databases

GeneIDi224829.
KEGGimmu:224829.
UCSCiuc008cur.1. mouse. [Q8BXJ2-1]
uc008cus.1. mouse. [Q8BXJ2-2]
uc008cut.1. mouse. [Q8BXJ2-3]
uc008cuw.1. mouse. [Q8BXJ2-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081774 mRNA. Translation: AAN02288.1. Frameshift.
AK042320 mRNA. Translation: BAC31222.1.
AK046822 mRNA. Translation: BAC32884.1.
BC051645 mRNA. Translation: AAH51645.1.
BC059215 mRNA. Translation: AAH59215.1.
BC108416 mRNA. Translation: AAI08417.1.
RefSeqiNP_001091092.1. NM_001097623.1. [Q8BXJ2-2]
NP_766210.1. NM_172622.2. [Q8BXJ2-1]
UniGeneiMm.260989.

3D structure databases

ProteinModelPortaliQ8BXJ2.
SMRiQ8BXJ2. Positions 1018-1044, 1091-1116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230328. 1 interaction.
IntActiQ8BXJ2. 1 interaction.
STRINGi10090.ENSMUSP00000077103.

PTM databases

iPTMnetiQ8BXJ2.
PhosphoSiteiQ8BXJ2.

Proteomic databases

MaxQBiQ8BXJ2.
PaxDbiQ8BXJ2.
PeptideAtlasiQ8BXJ2.
PRIDEiQ8BXJ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi224829.
KEGGimmu:224829.
UCSCiuc008cur.1. mouse. [Q8BXJ2-1]
uc008cus.1. mouse. [Q8BXJ2-2]
uc008cut.1. mouse. [Q8BXJ2-3]
uc008cuw.1. mouse. [Q8BXJ2-4]

Organism-specific databases

CTDi55809.
MGIiMGI:2442086. Trerf1.

Phylogenomic databases

eggNOGiKOG4167. Eukaryota.
ENOG4110BKZ. LUCA.
HOGENOMiHOG000154675.
HOVERGENiHBG082398.
InParanoidiQ8BXJ2.
PhylomeDBiQ8BXJ2.
TreeFamiTF106431.

Miscellaneous databases

PROiQ8BXJ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064043.
CleanExiMM_TRERF1.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR000949. ELM2_dom.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01448. ELM2. 1 hit.
PF13912. zf-C2H2_6. 3 hits.
[Graphical view]
SMARTiSM01189. ELM2. 1 hit.
SM00717. SANT. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51156. ELM2. 1 hit.
PS51293. SANT. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTREF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BXJ2
Secondary accession number(s): Q32NY5
, Q6PCQ4, Q80X25, Q810H8, Q8BY31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.