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Q8BXA7 (PHLP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PH domain leucine-rich repeat-containing protein phosphatase 2

EC=3.1.3.16
Alternative name(s):
PH domain leucine-rich repeat-containing protein phosphatase-like
Short name=PHLPP-like
Gene names
Name:Phlpp2
Synonyms:Phlppl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1320 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

Interacts with AKT1, AKT3 and PRKCB By similarity.

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein. Nucleus By similarity.

Sequence similarities

Contains 22 LRR (leucine-rich) repeats.

Contains 1 PH domain.

Contains 1 PP2C-like domain.

Sequence caution

The sequence BAC33347.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
Nucleus
   DiseaseTumor suppressor
   DomainLeucine-rich repeat
Repeat
   LigandManganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13201320PH domain leucine-rich repeat-containing protein phosphatase 2
PRO_0000057785

Regions

Domain147 – 24599PH
Repeat247 – 26822LRR 1
Repeat270 – 29324LRR 2
Repeat297 – 31822LRR 3
Repeat320 – 34122LRR 4
Repeat343 – 36422LRR 5
Repeat366 – 38823LRR 6
Repeat389 – 40921LRR 7
Repeat413 – 43422LRR 8
Repeat437 – 45721LRR 9
Repeat458 – 47720LRR 10
Repeat478 – 49922LRR 11
Repeat500 – 52122LRR 12
Repeat523 – 54422LRR 13
Repeat546 – 56722LRR 14
Repeat568 – 58922LRR 15
Repeat592 – 61322LRR 16
Repeat618 – 64124LRR 17
Repeat642 – 66322LRR 18
Repeat666 – 68722LRR 19
Repeat689 – 71022LRR 20
Repeat711 – 73222LRR 21
Repeat734 – 75522LRR 22
Domain772 – 1028257PP2C-like
Compositional bias40 – 478Poly-Thr
Compositional bias48 – 5710Poly-Ser
Compositional bias1068 – 10714Poly-Ser

Sites

Metal binding8171Manganese 1 By similarity
Metal binding8171Manganese 2 By similarity
Metal binding8181Manganese 1; via carbonyl oxygen By similarity
Metal binding9821Manganese 2 By similarity
Metal binding10211Manganese 2 By similarity

Experimental info

Sequence conflict671E → Q in BAC33288. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8BXA7 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 302407C231BD539B

FASTA1,320145,947
        10         20         30         40         50         60 
MKHNGSRTCL NRRSRFGSRE RDWLREDVKR GCVYLYGADT TTATTTTSSS SSSSSSSDLH 

        70         80         90        100        110        120 
LVLCTVETPA SEICAGEGRE SLYLQLHGDL VRRLEPSERP LQIVYDYLSR LGFEDPVRIQ 

       130        140        150        160        170        180 
EEATNPDLSC MIRFYGEKPC QMDHLDRILL SGIYNVRKGK TQLHKWAERL VVLCGTCLIV 

       190        200        210        220        230        240 
SSVKDCQTGK MHILPLVGGK IEEVKRRQHS LAFSSAGAQA QTYHVSFETL AEYQRWQRQA 

       250        260        270        280        290        300 
SKVVSQRMST VDLSCYSLEE VPEHLFYSQD ITYLNLRHNF MQLERPGGLD TLHKFSQLKG 

       310        320        330        340        350        360 
LNLSHNKLGL FPVLLCEIST LTELSLSCNG FHDLPSQIGK LLNLQTLSLD GNGLTALPDE 

       370        380        390        400        410        420 
LGNLRQLTSL GISFNDFRHI PEVLEKLTML DKVAMAGNRL EVLNLGALTR MSQVKHVDLR 

       430        440        450        460        470        480 
MNHLKTVITE NMEGNKHITH MDLRDNQLTD LDLSSLCSLE QLHCERNQLR ELTLSGFSLR 

       490        500        510        520        530        540 
TLYASWNRLT AVNVYPVPSL LTSLELSQNL LECVPDWACE AKKLEILDIS HNLLTEVPMR 

       550        560        570        580        590        600 
ILSSLSLRKL MVGHNHIHVL PALVEHIPLE VLDIQHNTLS RLPDTLFSKA LNLRYLNASA 

       610        620        630        640        650        660 
NSLESLPSAC AGEESLSVLQ LLYLTSNLLT DQCIPVLVGH PHLRVLHLAN NQLQTFPASK 

       670        680        690        700        710        720 
LNKLEQLEEL NLSGNKLTAI PTTIANCKRL HTLVAHANNI SIFPEILQLP QIQFVDLSCN 

       730        740        750        760        770        780 
DLTEILIPEA LPATLQDLDL TGNTNLVLEH KTLDMFSHIT ALKIDQKPLP ATDSAVTSTF 

       790        800        810        820        830        840 
WSHGLAEMAG QRNKLCVSAL AMDNFAEGVG AVYGMFDGDR NEELPRLLQC TMADVLLEEV 

       850        860        870        880        890        900 
QHSTNDTVFM TNTFLVSHRK LGMAGQKLGS SALLCYIRPD TADPTSSFSL TVANVGMCQA 

       910        920        930        940        950        960 
VLCRGGKPVP LSKVFSLEHD PEEAQRVKDQ KAIITEDNKV NGVTCCTRLL GCTYLYPWIL 

       970        980        990       1000       1010       1020 
PKPHIASTPL TIQDELLILG NKALWEHLSY LEAVNAVRHV QDPLAAAKKL CTLAQSYGCQ 

      1030       1040       1050       1060       1070       1080 
DNVGAMVVYL NIGEEGCTCE MNGLTLPGPV GFASTAALKD TPKPTTPSSS SGIASEFSSE 

      1090       1100       1110       1120       1130       1140 
MSTSEVSSEV GSTASDEHNT VGLEASLLPR PERRCSLHPA SSAGVFQRQP SCATFSSNQS 

      1150       1160       1170       1180       1190       1200 
DNGLDSDDDQ PVEGVITNGS RVEVEVDIHC CRGREPESSP PLPKNSSNAC SEERARGAGF 

      1210       1220       1230       1240       1250       1260 
GIRRQNSVNS GILLPANRDK MELQKSPSTS CLYGKKLSNG SIVPLEDSLN LIEVATEAPK 

      1270       1280       1290       1300       1310       1320 
RKTGYFAAPT QLEPEDQFVV PRDLEEEVKE QMKQHQEGRP EPEPRGEERT EPLEEFDTAL 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1259.
Strain: C57BL/6J.
Tissue: Head.
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC122807 Genomic DNA. No translation available.
BC117961 mRNA. Translation: AAI17962.1.
BC117962 mRNA. Translation: AAI17963.1.
AK048260 mRNA. Translation: BAC33288.1.
AK048472 mRNA. Translation: BAC33347.2. Different initiation.
RefSeqNP_001116066.2. NM_001122594.2.
UniGeneMm.23494.

3D structure databases

ProteinModelPortalQ8BXA7.
SMRQ8BXA7. Positions 248-763, 810-1030.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BXA7.

Proteomic databases

MaxQBQ8BXA7.
PaxDbQ8BXA7.
PRIDEQ8BXA7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034175; ENSMUSP00000034175; ENSMUSG00000031732.
GeneID244650.
KEGGmmu:244650.
UCSCuc009njn.2. mouse.

Organism-specific databases

CTD23035.
MGIMGI:2444928. Phlpp2.

Phylogenomic databases

eggNOGCOG4886.
GeneTreeENSGT00440000037833.
HOGENOMHOG000115529.
HOVERGENHBG062748.
InParanoidQ148U6.
KOK16340.
OrthoDBEOG7RFTGK.

Gene expression databases

ArrayExpressQ8BXA7.
BgeeQ8BXA7.
GenevestigatorQ8BXA7.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR011993. PH_like_dom.
IPR001932. PP2C-like_dom.
[Graphical view]
PfamPF12799. LRR_4. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS51450. LRR. 17 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPHLPP2. mouse.
NextBio386351.
PROQ8BXA7.
SOURCESearch...

Entry information

Entry namePHLP2_MOUSE
AccessionPrimary (citable) accession number: Q8BXA7
Secondary accession number(s): Q148U6, Q8BX96
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot