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Protein

Cytosolic endo-beta-N-acetylglucosaminidase

Gene

Engase

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol (By similarity).By similarity

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

GO - Biological processi

  • N-glycan processing Source: GO_Central
  • protein deglycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Short name:
ENGase
Gene namesi
Name:Engase
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2443788. Engase.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003288681 – 734Cytosolic endo-beta-N-acetylglucosaminidaseAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BX80.
PaxDbiQ8BX80.
PeptideAtlasiQ8BX80.
PRIDEiQ8BX80.

PTM databases

iPTMnetiQ8BX80.
PhosphoSitePlusiQ8BX80.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033857.
ExpressionAtlasiQ8BX80. baseline and differential.
GenevisibleiQ8BX80. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117538.

Structurei

3D structure databases

ProteinModelPortaliQ8BX80.
SMRiQ8BX80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 375BRCTPROSITE-ProRule annotationAdd BLAST95

Sequence similaritiesi

Belongs to the glycosyl hydrolase 85 family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2331. Eukaryota.
COG4724. LUCA.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8BX80.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG091G0A7Z.
PhylomeDBiQ8BX80.
TreeFamiTF314391.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR032979. ENGase.
IPR005201. Glyco_hydro_85.
[Graphical view]
PANTHERiPTHR13246. PTHR13246. 1 hit.
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BX80-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV
60 70 80 90 100
FREVVSFTPD PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL
110 120 130 140 150
EPLVCRRPPL SSPRPRTLLC HDMMGGYLED RFIQGSEVQN PYSFYHWQYI
160 170 180 190 200
DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL GTFITEWQEG GRLCEAFLAG
210 220 230 240 250
DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT PLFLQYLTAQ
260 270 280 290 300
LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE
310 320 330 340 350
DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA
360 370 380 390 400
ALFAPGWVYE CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL
410 420 430 440 450
GTRRVCYGKE QAVGPWYHPS AQETQPLFGE HKLAGDSRGW VKTHCCLTDA
460 470 480 490 500
WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP VPPKVFLSMV YKFEGSTDVQ
510 520 530 540 550
VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL ARWASSCGQQ
560 570 580 590 600
LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA
610 620 630 640 650
SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR
660 670 680 690 700
CFRIHCWKRT GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ
710 720 730
DGRVEFLVEP VPREGFLVPQ AEWGKAVLLF SVPQ
Length:734
Mass (Da):82,945
Last modified:March 1, 2003 - v1
Checksum:iAB95B9B7411F2EC1
GO
Isoform 2 (identifier: Q8BX80-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-45: INEDQ → CARLG
     46-734: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:45
Mass (Da):5,106
Checksum:iCE8CA3A17883599E
GO

Sequence cautioni

The sequence BAC39149 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03976341 – 45INEDQ → CARLG in isoform 2. 1 Publication5
Alternative sequenceiVSP_03976446 – 734Missing in isoform 2. 1 PublicationAdd BLAST689

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048667 mRNA. Translation: BAC33415.1.
AK167142 mRNA. Translation: BAE39287.1.
AK084254 mRNA. Translation: BAC39149.1. Sequence problems.
AL591075 Genomic DNA. Translation: CAM15758.1.
BC120924 mRNA. Translation: AAI20925.1.
CCDSiCCDS25704.1. [Q8BX80-1]
RefSeqiNP_766161.1. NM_172573.2. [Q8BX80-1]
UniGeneiMm.473963.

Genome annotation databases

EnsembliENSMUST00000043447; ENSMUSP00000126050; ENSMUSG00000033857. [Q8BX80-2]
ENSMUST00000135383; ENSMUSP00000117538; ENSMUSG00000033857. [Q8BX80-1]
ENSMUST00000172279; ENSMUSP00000130097; ENSMUSG00000033857. [Q8BX80-2]
GeneIDi217364.
KEGGimmu:217364.
UCSCiuc007mpi.1. mouse. [Q8BX80-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048667 mRNA. Translation: BAC33415.1.
AK167142 mRNA. Translation: BAE39287.1.
AK084254 mRNA. Translation: BAC39149.1. Sequence problems.
AL591075 Genomic DNA. Translation: CAM15758.1.
BC120924 mRNA. Translation: AAI20925.1.
CCDSiCCDS25704.1. [Q8BX80-1]
RefSeqiNP_766161.1. NM_172573.2. [Q8BX80-1]
UniGeneiMm.473963.

3D structure databases

ProteinModelPortaliQ8BX80.
SMRiQ8BX80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117538.

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

PTM databases

iPTMnetiQ8BX80.
PhosphoSitePlusiQ8BX80.

Proteomic databases

EPDiQ8BX80.
PaxDbiQ8BX80.
PeptideAtlasiQ8BX80.
PRIDEiQ8BX80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043447; ENSMUSP00000126050; ENSMUSG00000033857. [Q8BX80-2]
ENSMUST00000135383; ENSMUSP00000117538; ENSMUSG00000033857. [Q8BX80-1]
ENSMUST00000172279; ENSMUSP00000130097; ENSMUSG00000033857. [Q8BX80-2]
GeneIDi217364.
KEGGimmu:217364.
UCSCiuc007mpi.1. mouse. [Q8BX80-1]

Organism-specific databases

CTDi64772.
MGIiMGI:2443788. Engase.

Phylogenomic databases

eggNOGiKOG2331. Eukaryota.
COG4724. LUCA.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8BX80.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG091G0A7Z.
PhylomeDBiQ8BX80.
TreeFamiTF314391.

Enzyme and pathway databases

ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Miscellaneous databases

PROiQ8BX80.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033857.
ExpressionAtlasiQ8BX80. baseline and differential.
GenevisibleiQ8BX80. MM.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR032979. ENGase.
IPR005201. Glyco_hydro_85.
[Graphical view]
PANTHERiPTHR13246. PTHR13246. 1 hit.
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENASE_MOUSE
AccessioniPrimary (citable) accession number: Q8BX80
Secondary accession number(s): Q3TK58, Q8BJF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.