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Q8BX80 (ENASE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic endo-beta-N-acetylglucosaminidase
Short name=ENGase
EC=3.2.1.96
Gene names
Name:Engase
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.

Catalytic activity

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

Subcellular location

Cytoplasm › cytosol By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 85 family.

Contains 1 BRCT domain.

Sequence caution

The sequence BAC39149.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BX80-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BX80-2)

The sequence of this isoform differs from the canonical sequence as follows:
     41-45: INEDQ → CARLG
     46-734: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 734734Cytosolic endo-beta-N-acetylglucosaminidase
PRO_0000328868

Regions

Domain281 – 37595BRCT

Natural variations

Alternative sequence41 – 455INEDQ → CARLG in isoform 2.
VSP_039763
Alternative sequence46 – 734689Missing in isoform 2.
VSP_039764

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: AB95B9B7411F2EC1

FASTA73482,945
        10         20         30         40         50         60 
METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV FREVVSFTPD 

        70         80         90        100        110        120 
PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL EPLVCRRPPL SSPRPRTLLC 

       130        140        150        160        170        180 
HDMMGGYLED RFIQGSEVQN PYSFYHWQYI DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL 

       190        200        210        220        230        240 
GTFITEWQEG GRLCEAFLAG DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT 

       250        260        270        280        290        300 
PLFLQYLTAQ LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE 

       310        320        330        340        350        360 
DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA ALFAPGWVYE 

       370        380        390        400        410        420 
CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL GTRRVCYGKE QAVGPWYHPS 

       430        440        450        460        470        480 
AQETQPLFGE HKLAGDSRGW VKTHCCLTDA WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP 

       490        500        510        520        530        540 
VPPKVFLSMV YKFEGSTDVQ VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL 

       550        560        570        580        590        600 
ARWASSCGQQ LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA 

       610        620        630        640        650        660 
SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR CFRIHCWKRT 

       670        680        690        700        710        720 
GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ DGRVEFLVEP VPREGFLVPQ 

       730 
AEWGKAVLLF SVPQ

« Hide

Isoform 2 [UniParc].

Checksum: CE8CA3A17883599E
Show »

FASTA455,106

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Eye and Head.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK048667 mRNA. Translation: BAC33415.1.
AK167142 mRNA. Translation: BAE39287.1.
AK084254 mRNA. Translation: BAC39149.1. Sequence problems.
AL591075 Genomic DNA. Translation: CAM15758.1.
BC120924 mRNA. Translation: AAI20925.1.
IPIIPI00226541.
IPI00890299.
RefSeqNP_766161.1. NM_172573.2.
UniGeneMm.473963.

3D structure databases

ProteinModelPortalQ8BX80.
SMRQ8BX80. Positions 83-505.
ModBaseSearch...

Protein family/group databases

CAZyGH85. Glycoside Hydrolase Family 85.

PTM databases

PhosphoSiteQ8BX80.

Proteomic databases

PaxDbQ8BX80.
PRIDEQ8BX80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043447; ENSMUSP00000126050; ENSMUSG00000033857.
ENSMUST00000135383; ENSMUSP00000117538; ENSMUSG00000033857.
ENSMUST00000172279; ENSMUSP00000130097; ENSMUSG00000033857.
GeneID217364.
KEGGmmu:217364.
UCSCuc007mpi.1. mouse.

Organism-specific databases

CTD64772.
MGIMGI:2443788. Engase.

Phylogenomic databases

eggNOGCOG4724.
GeneTreeENSGT00390000018512.
HOGENOMHOG000082678.
HOVERGENHBG107848.
InParanoidQ8BX80.
KOK01227.
OMARCFRIHC.
OrthoDBEOG44J2HF.

Gene expression databases

ArrayExpressQ8BX80.
BgeeQ8BX80.
GenevestigatorQ8BX80.

Family and domain databases

InterProIPR001357. BRCT_dom.
IPR005201. Glyco_hydro_85.
[Graphical view]
PfamPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375811.
SOURCESearch...

Entry information

Entry nameENASE_MOUSE
AccessionPrimary (citable) accession number: Q8BX80
Secondary accession number(s): Q3TK58, Q8BJF1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents