Reviewed, UniProtKB/Swiss-Prot Q8BX80 (ENASE_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase
      Short name=ENGase
    EC=3.2.1.96

Gene names

Name:

Engase

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 85 family. Contains 1 BRCT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BX80-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BX80-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     559-561: CYE → HLL
     562-734: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Cytosolic endo-beta-N-acetylglucosaminidase		PRO_0000328868
Regions
	Domain	281 – 375	95	BRCT
Natural variations
	Alternative sequence	1 – 91	91	Missing in isoform 2.		VSP_032839
	Alternative sequence	559 – 561	3	CYE → HLL in isoform 2.		VSP_032840
	Alternative sequence	562 – 734	173	Missing in isoform 2.		VSP_032841

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: AB95B9B7411F2EC1	FASTA	734	82,945
	10 20 30 40 50 60 METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV FREVVSFTPD 70 80 90 100 110 120 PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL EPLVCRRPPL SSPRPRTLLC 130 140 150 160 170 180 HDMMGGYLED RFIQGSEVQN PYSFYHWQYI DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL 190 200 210 220 230 240 GTFITEWQEG GRLCEAFLAG DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT 250 260 270 280 290 300 PLFLQYLTAQ LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE 310 320 330 340 350 360 DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA ALFAPGWVYE 370 380 390 400 410 420 CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL GTRRVCYGKE QAVGPWYHPS 430 440 450 460 470 480 AQETQPLFGE HKLAGDSRGW VKTHCCLTDA WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP 490 500 510 520 530 540 VPPKVFLSMV YKFEGSTDVQ VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL 550 560 570 580 590 600 ARWASSCGQQ LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA 610 620 630 640 650 660 SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR CFRIHCWKRT 670 680 690 700 710 720 GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ DGRVEFLVEP VPREGFLVPQ 730 AEWGKAVLLF SVPQ
	Isoform 2. Checksum: 4586FE5BF3733E6D	FASTA	470	53,134
	10 20 30 40 50 60 MEDGFNVALE PLVCRRPPLS SPRPRTLLCH DMMGGYLEDR FIQGSEVQNP YSFYHWQYID 70 80 90 100 110 120 IFVYFSHHTV TIPPVCWTNA AHRHGVCVLG TFITEWQEGG RLCEAFLAGD EPSFQAVADR 130 140 150 160 170 180 LVQIAQFFRF DGWLINIENS LTPAAVRNTP LFLQYLTAQL HQQVPGGLVL WYDSVVQSGQ 190 200 210 220 230 240 LKWQDELNDQ NRVFFDSCDG FFTNYNWRED HLQRMVAQAG ERLADVYVGV DVFARSNVVG 250 260 270 280 290 300 GRFDTDKSLE LIRKHGFSAA LFAPGWVYEC LEKSDFFQNQ DKFWSLLERF LPTHSICSLP 310 320 330 340 350 360 FVTSFCLGLG TRRVCYGKEQ AVGPWYHPSA QETQPLFGEH KLAGDSRGWV KTHCCLTDAW 370 380 390 400 410 420 HGGSSLLLRG LIPPEVDSVA VRLFSLHIPV PPKVFLSMVY KFEGSTDVQV ALELTTGDAS 430 440 450 460 470 SCHVGGMLVL NETGSRHSPR PLRVPPTRLA RWASSCGQQL SGGWIQRHLL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Eye and Head.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK048667 mRNA. Translation: BAC33415.1. AK167142 mRNA. Translation: BAE39287.1. AK084254 mRNA. Translation: BAC39149.1. AL591075 Genomic DNA. Translation: CAM15758.1. BC120924 mRNA. Translation: AAI20925.1.
IPI	IPI00226541. IPI00890299.
RefSeq	NP_766161.1.
UniGene	Mm.473963
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BX80.
Protein family/group databases
CAZy	GH85. Glycoside Hydrolase Family 85.
Proteomic databases
PRIDE	Q8BX80.
Genome annotation databases
Ensembl	ENSMUST00000043447; ENSMUSP00000048272; ENSMUSG00000033857; Mus musculus. [Genome view]
GeneID	217364.
KEGG	mmu:217364.
Organism-specific databases
CTD	217364.
MGI	MGI:2443788. D230014K01Rik.
Phylogenomic databases
HOGENOM	Q8BX80.
HOVERGEN	Q8BX80.
OMA	FDTDKSL
OrthoDB	EOG99PDJ2
Enzyme and pathway databases
BRENDA	3.2.1.96. 244.
Gene expression databases
Genevestigator	Q8BX80.
Family and domain databases
InterPro	IPR001357. BRCT. IPR005201. Glyco_hydro_85. [Graphical view]
PANTHER	PTHR13246. Glyco_hydro_85. 1 hit.
Pfam	PF03644. Glyco_hydro_85. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	375811.
SOURCE	Search...

Entry information

Entry name

ENASE_MOUSE

Accession

Primary (citable) accession number: Q8BX80
Secondary accession number(s): Q3TK58, Q8BJF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)