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Q8BX80

- ENASE_MOUSE

UniProt

Q8BX80 - ENASE_MOUSE

Protein

Cytosolic endo-beta-N-acetylglucosaminidase

Gene

Engase

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.By similarity

    Catalytic activityi

    Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

    GO - Molecular functioni

    1. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH85. Glycoside Hydrolase Family 85.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
    Short name:
    ENGase
    Gene namesi
    Name:Engase
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2443788. Engase.

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 734734Cytosolic endo-beta-N-acetylglucosaminidasePRO_0000328868Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8BX80.
    PaxDbiQ8BX80.
    PRIDEiQ8BX80.

    PTM databases

    PhosphoSiteiQ8BX80.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BX80.
    BgeeiQ8BX80.
    GenevestigatoriQ8BX80.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BX80.
    SMRiQ8BX80. Positions 83-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini281 – 37595BRCTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 85 family.Curated
    Contains 1 BRCT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4724.
    GeneTreeiENSGT00390000018512.
    HOGENOMiHOG000082678.
    HOVERGENiHBG107848.
    InParanoidiQ8BX80.
    KOiK01227.
    OMAiPTKLARW.
    OrthoDBiEOG7KQ218.
    PhylomeDBiQ8BX80.
    TreeFamiTF314391.

    Family and domain databases

    InterProiIPR001357. BRCT_dom.
    IPR005201. Glyco_hydro_85.
    [Graphical view]
    PfamiPF03644. Glyco_hydro_85. 1 hit.
    [Graphical view]
    PROSITEiPS50172. BRCT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BX80-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV    50
    FREVVSFTPD PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL 100
    EPLVCRRPPL SSPRPRTLLC HDMMGGYLED RFIQGSEVQN PYSFYHWQYI 150
    DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL GTFITEWQEG GRLCEAFLAG 200
    DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT PLFLQYLTAQ 250
    LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE 300
    DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA 350
    ALFAPGWVYE CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL 400
    GTRRVCYGKE QAVGPWYHPS AQETQPLFGE HKLAGDSRGW VKTHCCLTDA 450
    WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP VPPKVFLSMV YKFEGSTDVQ 500
    VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL ARWASSCGQQ 550
    LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA 600
    SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR 650
    CFRIHCWKRT GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ 700
    DGRVEFLVEP VPREGFLVPQ AEWGKAVLLF SVPQ 734
    Length:734
    Mass (Da):82,945
    Last modified:March 1, 2003 - v1
    Checksum:iAB95B9B7411F2EC1
    GO
    Isoform 2 (identifier: Q8BX80-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         41-45: INEDQ → CARLG
         46-734: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:45
    Mass (Da):5,106
    Checksum:iCE8CA3A17883599E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei41 – 455INEDQ → CARLG in isoform 2. 1 PublicationVSP_039763
    Alternative sequencei46 – 734689Missing in isoform 2. 1 PublicationVSP_039764Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK048667 mRNA. Translation: BAC33415.1.
    AK167142 mRNA. Translation: BAE39287.1.
    AK084254 mRNA. Translation: BAC39149.1. Sequence problems.
    AL591075 Genomic DNA. Translation: CAM15758.1.
    BC120924 mRNA. Translation: AAI20925.1.
    CCDSiCCDS25704.1. [Q8BX80-1]
    RefSeqiNP_766161.1. NM_172573.2. [Q8BX80-1]
    UniGeneiMm.473963.

    Genome annotation databases

    EnsembliENSMUST00000043447; ENSMUSP00000126050; ENSMUSG00000033857. [Q8BX80-2]
    ENSMUST00000135383; ENSMUSP00000117538; ENSMUSG00000033857. [Q8BX80-1]
    ENSMUST00000172279; ENSMUSP00000130097; ENSMUSG00000033857. [Q8BX80-2]
    GeneIDi217364.
    KEGGimmu:217364.
    UCSCiuc007mpi.1. mouse. [Q8BX80-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK048667 mRNA. Translation: BAC33415.1 .
    AK167142 mRNA. Translation: BAE39287.1 .
    AK084254 mRNA. Translation: BAC39149.1 . Sequence problems.
    AL591075 Genomic DNA. Translation: CAM15758.1 .
    BC120924 mRNA. Translation: AAI20925.1 .
    CCDSi CCDS25704.1. [Q8BX80-1 ]
    RefSeqi NP_766161.1. NM_172573.2. [Q8BX80-1 ]
    UniGenei Mm.473963.

    3D structure databases

    ProteinModelPortali Q8BX80.
    SMRi Q8BX80. Positions 83-505.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH85. Glycoside Hydrolase Family 85.

    PTM databases

    PhosphoSitei Q8BX80.

    Proteomic databases

    MaxQBi Q8BX80.
    PaxDbi Q8BX80.
    PRIDEi Q8BX80.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043447 ; ENSMUSP00000126050 ; ENSMUSG00000033857 . [Q8BX80-2 ]
    ENSMUST00000135383 ; ENSMUSP00000117538 ; ENSMUSG00000033857 . [Q8BX80-1 ]
    ENSMUST00000172279 ; ENSMUSP00000130097 ; ENSMUSG00000033857 . [Q8BX80-2 ]
    GeneIDi 217364.
    KEGGi mmu:217364.
    UCSCi uc007mpi.1. mouse. [Q8BX80-1 ]

    Organism-specific databases

    CTDi 64772.
    MGIi MGI:2443788. Engase.

    Phylogenomic databases

    eggNOGi COG4724.
    GeneTreei ENSGT00390000018512.
    HOGENOMi HOG000082678.
    HOVERGENi HBG107848.
    InParanoidi Q8BX80.
    KOi K01227.
    OMAi PTKLARW.
    OrthoDBi EOG7KQ218.
    PhylomeDBi Q8BX80.
    TreeFami TF314391.

    Miscellaneous databases

    NextBioi 375811.
    PROi Q8BX80.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BX80.
    Bgeei Q8BX80.
    Genevestigatori Q8BX80.

    Family and domain databases

    InterProi IPR001357. BRCT_dom.
    IPR005201. Glyco_hydro_85.
    [Graphical view ]
    Pfami PF03644. Glyco_hydro_85. 1 hit.
    [Graphical view ]
    PROSITEi PS50172. BRCT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Eye and Head.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiENASE_MOUSE
    AccessioniPrimary (citable) accession number: Q8BX80
    Secondary accession number(s): Q3TK58, Q8BJF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3