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Q8BX80

- ENASE_MOUSE

UniProt

Q8BX80 - ENASE_MOUSE

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Protein
Cytosolic endo-beta-N-acetylglucosaminidase
Gene
Engase
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

  1. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH85. Glycoside Hydrolase Family 85.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Short name:
ENGase
Gene namesi
Name:Engase
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2443788. Engase.

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734Cytosolic endo-beta-N-acetylglucosaminidase
PRO_0000328868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BX80.
PaxDbiQ8BX80.
PRIDEiQ8BX80.

PTM databases

PhosphoSiteiQ8BX80.

Expressioni

Gene expression databases

ArrayExpressiQ8BX80.
BgeeiQ8BX80.
GenevestigatoriQ8BX80.

Structurei

3D structure databases

ProteinModelPortaliQ8BX80.
SMRiQ8BX80. Positions 83-505.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini281 – 37595BRCT
Add
BLAST

Sequence similaritiesi

Contains 1 BRCT domain.

Phylogenomic databases

eggNOGiCOG4724.
GeneTreeiENSGT00390000018512.
HOGENOMiHOG000082678.
HOVERGENiHBG107848.
InParanoidiQ8BX80.
KOiK01227.
OMAiPTKLARW.
OrthoDBiEOG7KQ218.
PhylomeDBiQ8BX80.
TreeFamiTF314391.

Family and domain databases

InterProiIPR001357. BRCT_dom.
IPR005201. Glyco_hydro_85.
[Graphical view]
PfamiPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BX80-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV    50
FREVVSFTPD PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL 100
EPLVCRRPPL SSPRPRTLLC HDMMGGYLED RFIQGSEVQN PYSFYHWQYI 150
DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL GTFITEWQEG GRLCEAFLAG 200
DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT PLFLQYLTAQ 250
LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE 300
DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA 350
ALFAPGWVYE CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL 400
GTRRVCYGKE QAVGPWYHPS AQETQPLFGE HKLAGDSRGW VKTHCCLTDA 450
WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP VPPKVFLSMV YKFEGSTDVQ 500
VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL ARWASSCGQQ 550
LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA 600
SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR 650
CFRIHCWKRT GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ 700
DGRVEFLVEP VPREGFLVPQ AEWGKAVLLF SVPQ 734
Length:734
Mass (Da):82,945
Last modified:March 1, 2003 - v1
Checksum:iAB95B9B7411F2EC1
GO
Isoform 2 (identifier: Q8BX80-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-45: INEDQ → CARLG
     46-734: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Show »
Length:45
Mass (Da):5,106
Checksum:iCE8CA3A17883599E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei41 – 455INEDQ → CARLG in isoform 2.
VSP_039763
Alternative sequencei46 – 734689Missing in isoform 2.
VSP_039764Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK048667 mRNA. Translation: BAC33415.1.
AK167142 mRNA. Translation: BAE39287.1.
AK084254 mRNA. Translation: BAC39149.1. Sequence problems.
AL591075 Genomic DNA. Translation: CAM15758.1.
BC120924 mRNA. Translation: AAI20925.1.
CCDSiCCDS25704.1. [Q8BX80-1]
RefSeqiNP_766161.1. NM_172573.2. [Q8BX80-1]
UniGeneiMm.473963.

Genome annotation databases

EnsembliENSMUST00000043447; ENSMUSP00000126050; ENSMUSG00000033857. [Q8BX80-2]
ENSMUST00000135383; ENSMUSP00000117538; ENSMUSG00000033857. [Q8BX80-1]
ENSMUST00000172279; ENSMUSP00000130097; ENSMUSG00000033857. [Q8BX80-2]
GeneIDi217364.
KEGGimmu:217364.
UCSCiuc007mpi.1. mouse. [Q8BX80-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK048667 mRNA. Translation: BAC33415.1 .
AK167142 mRNA. Translation: BAE39287.1 .
AK084254 mRNA. Translation: BAC39149.1 . Sequence problems.
AL591075 Genomic DNA. Translation: CAM15758.1 .
BC120924 mRNA. Translation: AAI20925.1 .
CCDSi CCDS25704.1. [Q8BX80-1 ]
RefSeqi NP_766161.1. NM_172573.2. [Q8BX80-1 ]
UniGenei Mm.473963.

3D structure databases

ProteinModelPortali Q8BX80.
SMRi Q8BX80. Positions 83-505.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH85. Glycoside Hydrolase Family 85.

PTM databases

PhosphoSitei Q8BX80.

Proteomic databases

MaxQBi Q8BX80.
PaxDbi Q8BX80.
PRIDEi Q8BX80.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043447 ; ENSMUSP00000126050 ; ENSMUSG00000033857 . [Q8BX80-2 ]
ENSMUST00000135383 ; ENSMUSP00000117538 ; ENSMUSG00000033857 . [Q8BX80-1 ]
ENSMUST00000172279 ; ENSMUSP00000130097 ; ENSMUSG00000033857 . [Q8BX80-2 ]
GeneIDi 217364.
KEGGi mmu:217364.
UCSCi uc007mpi.1. mouse. [Q8BX80-1 ]

Organism-specific databases

CTDi 64772.
MGIi MGI:2443788. Engase.

Phylogenomic databases

eggNOGi COG4724.
GeneTreei ENSGT00390000018512.
HOGENOMi HOG000082678.
HOVERGENi HBG107848.
InParanoidi Q8BX80.
KOi K01227.
OMAi PTKLARW.
OrthoDBi EOG7KQ218.
PhylomeDBi Q8BX80.
TreeFami TF314391.

Miscellaneous databases

NextBioi 375811.
PROi Q8BX80.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BX80.
Bgeei Q8BX80.
Genevestigatori Q8BX80.

Family and domain databases

InterProi IPR001357. BRCT_dom.
IPR005201. Glyco_hydro_85.
[Graphical view ]
Pfami PF03644. Glyco_hydro_85. 1 hit.
[Graphical view ]
PROSITEi PS50172. BRCT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Eye and Head.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiENASE_MOUSE
AccessioniPrimary (citable) accession number: Q8BX80
Secondary accession number(s): Q3TK58, Q8BJF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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