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Protein

MyoD family inhibitor domain-containing protein

Gene

Mdfic

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from cellular promoters. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin-regulation of the WNT and JNK signaling pathways.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
MyoD family inhibitor domain-containing protein
Alternative name(s):
I-mfa domain-containing protein
Kidney cell line-derived transcript 1
Gene namesi
Name:MdficImported
Synonyms:Kdt1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:104611. Mdfic.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: MGI
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247MyoD family inhibitor domain-containing proteinPRO_0000280223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei141 – 1411PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BX65.
PaxDbiQ8BX65.
PRIDEiQ8BX65.

PTM databases

iPTMnetiQ8BX65.
PhosphoSiteiQ8BX65.

Expressioni

Gene expression databases

BgeeiQ8BX65.
CleanExiMM_MDFIC.
ExpressionAtlasiQ8BX65. baseline and differential.
GenevisibleiQ8BX65. MM.

Interactioni

Subunit structurei

Interacts with HAND1; leading to sequester HAND1 into the nucleolus and prevent its activity (PubMed:17891141). Interacts with ZIC2 (PubMed:15207726, PubMed:15465018). The C-terminus interacts with HIV-1 Tat and Rev, AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with LEF1. Interacts with CCNT2 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BX65. 1 interaction.
STRINGi10090.ENSMUSP00000099186.

Structurei

3D structure databases

ProteinModelPortaliQ8BX65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi167 – 24478Cys-richSequence analysisAdd
BLAST

Domaini

The cysteine-rich C-terminus is involved in its granular distribution in the cytoplasm (By similarity). The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.By similarity

Sequence similaritiesi

Belongs to the MDFI family.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IKRS. Eukaryota.
ENOG4111J6Q. LUCA.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
HOVERGENiHBG068212.
InParanoidiQ8BX65.
OrthoDBiEOG70088T.
PhylomeDBiQ8BX65.
TreeFamiTF332113.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.
PfamiPF15316. MDFI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q8BX65-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCAGEALAP GPAEQQCPVE AGGGRLGSPA HEACNEDNTE KDKRPATSGH
60 70 80 90 100
TRCGLMRDQS IWPNPSAGEL VRTQPERLPQ LQTSAQEPGK EETGKIKNGG
110 120 130 140 150
HTRMSNGNGI PHGAKHVSVE NHKISAPVSQ KMHRKIQSSL SVNNDISKKS
160 170 180 190 200
KVNAVFSPKA ASSPEDCCVH CILACLFCEF LTLCNIVLGQ ASCGICTSEA
210 220 230 240
CCCCCGDEMG DDCSCPCDMD CGIMDACCES SDCLEICMEC CGICFPS
Length:247
Mass (Da):26,094
Last modified:March 1, 2003 - v1
Checksum:iB4FBA7F07565E3B2
GO
Isoform 21 Publication (identifier: Q8BX65-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     32-72: Missing.
     73-73: T → A

Note: No experimental confirmation available.Curated
Show »
Length:206
Mass (Da):21,511
Checksum:i616436F932B6AC0E
GO

Sequence cautioni

The sequence AAH67006.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC37840.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE43047.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei32 – 7241Missing in isoform 2. 1 PublicationVSP_052336Add
BLAST
Alternative sequencei73 – 731T → A in isoform 2. 1 PublicationVSP_052337

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048821 mRNA. Translation: BAC33469.1.
AK080178 mRNA. Translation: BAC37840.1. Different initiation.
AK165016 mRNA. Translation: BAE38001.1.
AK172522 mRNA. Translation: BAE43047.1. Different initiation.
BC067006 mRNA. Translation: AAH67006.1. Different initiation.
CCDSiCCDS19919.1. [Q8BX65-1]
RefSeqiNP_780297.3. NM_175088.5. [Q8BX65-1]
XP_006505061.1. XM_006504998.2. [Q8BX65-1]
UniGeneiMm.1314.

Genome annotation databases

EnsembliENSMUST00000101663; ENSMUSP00000099186; ENSMUSG00000041390. [Q8BX65-1]
ENSMUST00000120512; ENSMUSP00000113050; ENSMUSG00000041390. [Q8BX65-1]
ENSMUST00000189359; ENSMUSP00000140208; ENSMUSG00000041390. [Q8BX65-1]
GeneIDi16543.
KEGGimmu:16543.
UCSCiuc009azc.2. mouse. [Q8BX65-1]
uc012eif.1. mouse. [Q8BX65-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048821 mRNA. Translation: BAC33469.1.
AK080178 mRNA. Translation: BAC37840.1. Different initiation.
AK165016 mRNA. Translation: BAE38001.1.
AK172522 mRNA. Translation: BAE43047.1. Different initiation.
BC067006 mRNA. Translation: AAH67006.1. Different initiation.
CCDSiCCDS19919.1. [Q8BX65-1]
RefSeqiNP_780297.3. NM_175088.5. [Q8BX65-1]
XP_006505061.1. XM_006504998.2. [Q8BX65-1]
UniGeneiMm.1314.

3D structure databases

ProteinModelPortaliQ8BX65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BX65. 1 interaction.
STRINGi10090.ENSMUSP00000099186.

PTM databases

iPTMnetiQ8BX65.
PhosphoSiteiQ8BX65.

Proteomic databases

MaxQBiQ8BX65.
PaxDbiQ8BX65.
PRIDEiQ8BX65.

Protocols and materials databases

DNASUi16543.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101663; ENSMUSP00000099186; ENSMUSG00000041390. [Q8BX65-1]
ENSMUST00000120512; ENSMUSP00000113050; ENSMUSG00000041390. [Q8BX65-1]
ENSMUST00000189359; ENSMUSP00000140208; ENSMUSG00000041390. [Q8BX65-1]
GeneIDi16543.
KEGGimmu:16543.
UCSCiuc009azc.2. mouse. [Q8BX65-1]
uc012eif.1. mouse. [Q8BX65-2]

Organism-specific databases

CTDi29969.
MGIiMGI:104611. Mdfic.

Phylogenomic databases

eggNOGiENOG410IKRS. Eukaryota.
ENOG4111J6Q. LUCA.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
HOVERGENiHBG068212.
InParanoidiQ8BX65.
OrthoDBiEOG70088T.
PhylomeDBiQ8BX65.
TreeFamiTF332113.

Miscellaneous databases

PROiQ8BX65.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BX65.
CleanExiMM_MDFIC.
ExpressionAtlasiQ8BX65. baseline and differential.
GenevisibleiQ8BX65. MM.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.
PfamiPF15316. MDFI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Aorta, Cerebellum, Eye, Spleen and Vein.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  3. "Myogenic repressor I-mfa interferes with the function of Zic family proteins."
    Mizugishi K., Hatayama M., Tohmonda T., Ogawa M., Inoue T., Mikoshiba K., Aruga J.
    Biochem. Biophys. Res. Commun. 320:233-240(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ZIC2, SUBCELLULAR LOCATION.
  4. "Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family."
    Ishiguro A., Inoue T., Mikoshiba K., Aruga J.
    Biochem. Biophys. Res. Commun. 324:302-307(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ZIC2, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH HAND1.

Entry informationi

Entry nameiMDFIC_MOUSE
AccessioniPrimary (citable) accession number: Q8BX65
Secondary accession number(s): Q3T9H3, Q6NXM1, Q8BJS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.