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Protein

PX domain-containing protein kinase-like protein

Gene

Pxk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.By similarity1 Publication

GO - Molecular functioni

  • ATP binding Source: InterPro
  • phosphatidylinositol binding Source: InterPro
  • protein C-terminus binding Source: UniProtKB
  • protein kinase activity Source: InterPro

GO - Biological processi

  • inflammatory response Source: MGI
  • modulation of synaptic transmission Source: UniProtKB
  • negative regulation of ATPase activity Source: UniProtKB
  • negative regulation of ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PX domain-containing protein kinase-like protein
Alternative name(s):
Modulator of Na,K-ATPase
Short name:
MONaKA
Gene namesi
Name:PxkImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1289230. Pxk.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • microtubule organizing center Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582PX domain-containing protein kinase-like proteinPRO_0000086593Add
BLAST

Proteomic databases

EPDiQ8BX57.
MaxQBiQ8BX57.
PaxDbiQ8BX57.
PRIDEiQ8BX57.

PTM databases

iPTMnetiQ8BX57.
PhosphoSiteiQ8BX57.

Expressioni

Tissue specificityi

Isoform 1 is present in all tissues examined. Isoform 2 is found in all tissues except skeletal muscle and very low levels in spleen. Both isoforms are widely expressed throughout the nervous system however levels of isoform 2 are higher in purified hippocampal and cortical neurons whereas glial cells express more isoform 1 than isoform 2.1 Publication

Gene expression databases

BgeeiQ8BX57.
CleanExiMM_PXK.
GenevisibleiQ8BX57. MM.

Interactioni

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8BX57. 1 interaction.
MINTiMINT-4997667.
STRINGi10090.ENSMUSP00000035265.

Structurei

3D structure databases

ProteinModelPortaliQ8BX57.
SMRiQ8BX57. Positions 33-119, 146-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 126113PXPROSITE-ProRule annotationAdd
BLAST
Domaini88 – 481394Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini548 – 56720WH2PROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF7B. Eukaryota.
ENOG410Z20X. LUCA.
GeneTreeiENSGT00390000017669.
HOVERGENiHBG061245.
InParanoidiQ8BX57.
KOiK17543.
OMAiLESICKF.
OrthoDBiEOG7FFMRQ.
PhylomeDBiQ8BX57.
TreeFamiTF324116.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8BX57-1) [UniParc]FASTAAdd to basket

Also known as: Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFMEKPPAG KVLLDDTVPL TAAVEASQSL QSHTEYIIRV QRGISAENSW
60 70 80 90 100
QIVRRYSDFD LLNNSLQITG LSLPLPPKKL IGNMDREFIA ERQRGLQNYL
110 120 130 140 150
NVIMANHVLS NCELLKKFLD PNNYSANYTE IALQQVSMFF RSEPKWEVVE
160 170 180 190 200
PLKDIGWRIR KKYFLMKIKN QPKERLVLSW ADLGPDKYLS DKDFQCLIKL
210 220 230 240 250
LPSCVHPYIY RVTFATASES SALLIRAFNE KGTLKDLIYK AKPKDPFLKK
260 270 280 290 300
YCNPKKTQGL ELQQIKTYGR QILEALKFLH DKGFPYGHLH AANVMLDGNT
310 320 330 340 350
CRLLDLENSL LGLPSFYRSY FTQFRKINTL ESVDVHCFGH LLYEMTYGRP
360 370 380 390 400
PDSVPVDSFP PASSLAVVAV LESTLSCEAC KNGMPTVSRL LQMPLFSDVL
410 420 430 440 450
LTTSEKPQFK IPTKLKEALR IAKECIEKRL TEEQKQIHQH RRLTRAQSHH
460 470 480 490 500
GSEEERKRRK ILARKKSKRS AVENSEEQPV KHSNSNNSAG SGASSPLTSP
510 520 530 540 550
SSPTPPSTAG LSSALPPPPP PPPPPPPPAG PSPTSATEMP APFLPQPVNG
560 570 580
VNRGALLSSI QNFQKGTLRK AQTCDHSAPK IG
Length:582
Mass (Da):65,231
Last modified:December 6, 2005 - v2
Checksum:iED554975AECE04AA
GO
Isoform 21 Publication (identifier: Q8BX57-2) [UniParc]FASTAAdd to basket

Also known as: Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     510-515: GLSSAL → VEHAPL
     516-582: Missing.

Show »
Length:515
Mass (Da):58,517
Checksum:iC070286129A85F31
GO
Isoform 3Curated (identifier: Q8BX57-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-448: VAVLESTLSC...QHRRLTRAQS → GQYRAWQWGV...WKENCGQLGF
     449-582: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:448
Mass (Da):50,958
Checksum:i3CD6F498CFA00126
GO

Sequence cautioni

The sequence BAE33603.1 differs from that shown.Intron retention.Curated
The sequence BAE33603.1 differs from that shown. Reason: Frameshift at position 511. Curated
The sequence BAE34065.1 differs from that shown. Reason: Frameshift at position 575. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441K → R in BAE34065 (PubMed:16141072).Curated
Sequence conflicti306 – 3061L → P in BAE30914 (PubMed:16141072).Curated
Sequence conflicti306 – 3061L → P in BAE31498 (PubMed:16141072).Curated
Sequence conflicti306 – 3061L → P in BAE31693 (PubMed:16141072).Curated
Sequence conflicti489 – 4891Missing in AAZ04665 (PubMed:16135750).Curated
Sequence conflicti489 – 4891Missing in BAC33489 (PubMed:16141072).Curated
Sequence conflicti489 – 4891Missing in BAE29479 (PubMed:16141072).Curated
Sequence conflicti489 – 4891Missing in BAE29537 (PubMed:16141072).Curated
Sequence conflicti489 – 4891Missing in BAE41744 (PubMed:16141072).Curated
Sequence conflicti489 – 4891Missing in BAE34065 (PubMed:16141072).Curated
Sequence conflicti523 – 5231P → R in BAE41744 (PubMed:16141072).Curated
Sequence conflicti539 – 5391M → T in BAE34065 (PubMed:16141072).Curated
Sequence conflicti539 – 5391M → T in AAH16131 (PubMed:15489334).Curated
Sequence conflicti579 – 5791P → S in BAE29537 (PubMed:16141072).Curated
Sequence conflicti579 – 5791P → S in BAE29479 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei368 – 44881VAVLE…TRAQS → GQYRAWQWGVLGSGSLQSCL SAYQTPRRMEGQLSCSPARA LNMWMVCLSVHLSEASGETS VNCTVILKAGQWKENCGQLG F in isoform 3. 1 PublicationVSP_051914Add
BLAST
Alternative sequencei449 – 582134Missing in isoform 3. 1 PublicationVSP_051915Add
BLAST
Alternative sequencei510 – 5156GLSSAL → VEHAPL in isoform 2. 1 PublicationVSP_051916
Alternative sequencei516 – 58267Missing in isoform 2. 1 PublicationVSP_051917Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ095196 mRNA. Translation: AAZ04664.1.
DQ095197 mRNA. Translation: AAZ04665.1.
AK048910 mRNA. Translation: BAC33489.1.
AK152058 mRNA. Translation: BAE30914.1.
AK152789 mRNA. Translation: BAE31498.1.
AK153067 mRNA. Translation: BAE31693.1.
AK156146 mRNA. Translation: BAE33603.1. Frameshift.
AK157364 mRNA. Translation: BAE34065.1. Frameshift.
AK169565 mRNA. Translation: BAE41231.1.
AK170361 mRNA. Translation: BAE41744.1.
AK150413 mRNA. Translation: BAE29537.1.
AK150337 mRNA. Translation: BAE29479.1.
BC016131 mRNA. Translation: AAH16131.1.
CCDSiCCDS26810.1. [Q8BX57-1]
CCDS36799.1. [Q8BX57-2]
RefSeqiNP_663433.2. NM_145458.3. [Q8BX57-1]
NP_840063.1. NM_178279.2. [Q8BX57-2]
XP_006518039.1. XM_006517976.2.
XP_006518042.1. XM_006517979.2. [Q8BX57-2]
UniGeneiMm.29515.

Genome annotation databases

EnsembliENSMUST00000036682; ENSMUSP00000035265; ENSMUSG00000033885. [Q8BX57-1]
ENSMUST00000112689; ENSMUSP00000108309; ENSMUSG00000033885. [Q8BX57-2]
GeneIDi218699.
KEGGimmu:218699.
UCSCiuc007ser.2. mouse. [Q8BX57-3]
uc007ses.2. mouse. [Q8BX57-2]
uc007set.2. mouse. [Q8BX57-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ095196 mRNA. Translation: AAZ04664.1.
DQ095197 mRNA. Translation: AAZ04665.1.
AK048910 mRNA. Translation: BAC33489.1.
AK152058 mRNA. Translation: BAE30914.1.
AK152789 mRNA. Translation: BAE31498.1.
AK153067 mRNA. Translation: BAE31693.1.
AK156146 mRNA. Translation: BAE33603.1. Frameshift.
AK157364 mRNA. Translation: BAE34065.1. Frameshift.
AK169565 mRNA. Translation: BAE41231.1.
AK170361 mRNA. Translation: BAE41744.1.
AK150413 mRNA. Translation: BAE29537.1.
AK150337 mRNA. Translation: BAE29479.1.
BC016131 mRNA. Translation: AAH16131.1.
CCDSiCCDS26810.1. [Q8BX57-1]
CCDS36799.1. [Q8BX57-2]
RefSeqiNP_663433.2. NM_145458.3. [Q8BX57-1]
NP_840063.1. NM_178279.2. [Q8BX57-2]
XP_006518039.1. XM_006517976.2.
XP_006518042.1. XM_006517979.2. [Q8BX57-2]
UniGeneiMm.29515.

3D structure databases

ProteinModelPortaliQ8BX57.
SMRiQ8BX57. Positions 33-119, 146-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BX57. 1 interaction.
MINTiMINT-4997667.
STRINGi10090.ENSMUSP00000035265.

PTM databases

iPTMnetiQ8BX57.
PhosphoSiteiQ8BX57.

Proteomic databases

EPDiQ8BX57.
MaxQBiQ8BX57.
PaxDbiQ8BX57.
PRIDEiQ8BX57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036682; ENSMUSP00000035265; ENSMUSG00000033885. [Q8BX57-1]
ENSMUST00000112689; ENSMUSP00000108309; ENSMUSG00000033885. [Q8BX57-2]
GeneIDi218699.
KEGGimmu:218699.
UCSCiuc007ser.2. mouse. [Q8BX57-3]
uc007ses.2. mouse. [Q8BX57-2]
uc007set.2. mouse. [Q8BX57-1]

Organism-specific databases

CTDi54899.
MGIiMGI:1289230. Pxk.

Phylogenomic databases

eggNOGiENOG410IF7B. Eukaryota.
ENOG410Z20X. LUCA.
GeneTreeiENSGT00390000017669.
HOVERGENiHBG061245.
InParanoidiQ8BX57.
KOiK17543.
OMAiLESICKF.
OrthoDBiEOG7FFMRQ.
PhylomeDBiQ8BX57.
TreeFamiTF324116.

Miscellaneous databases

PROiQ8BX57.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BX57.
CleanExiMM_PXK.
GenevisibleiQ8BX57. MM.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
IPR003124. WH2_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MONaKA, a novel modulator of the plasma membrane Na,K-ATPase."
    Mao H., Ferguson T.S., Cibulsky S.M., Holmqvist M., Ding C., Fei H., Levitan I.B.
    J. Neurosci. 25:7934-7943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ATP1B1 AND ATP1B3.
    Strain: BALB/cJImported.
    Tissue: Brain1 Publication.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6JImported and NODImported.
    Tissue: Bone marrowImported, CerebellumImported, SpleenImported and ThymusImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/NImported.
    Tissue: Salivary glandImported.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Spleen.

Entry informationi

Entry nameiPXK_MOUSE
AccessioniPrimary (citable) accession number: Q8BX57
Secondary accession number(s): Q3TD60
, Q3TEL7, Q3TZZ1, Q3U197, Q3U773, Q3UCS5, Q4FBH7, Q91WB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 8, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.