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Protein

Sal-like protein 4

Gene

Sall4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri387 – 409C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri415 – 437C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri573 – 595C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri601 – 623C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri633 – 655C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri880 – 902C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri908 – 930C2H2-type 7PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • embryonic limb morphogenesis Source: MGI
  • heart development Source: MGI
  • inner cell mass cell proliferation Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neural tube closure Source: MGI
  • neural tube development Source: MGI
  • neurogenesis Source: GO_Central
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • signal transduction Source: GO_Central
  • stem cell population maintenance Source: MGI
  • tissue development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventricular septum development Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8943724. Regulation of PTEN gene transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Sal-like protein 4
Alternative name(s):
Zinc finger protein SALL4
Gene namesi
Name:Sall4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2139360. Sall4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002614161 – 1067Sal-like protein 4Add BLAST1067

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Cross-linki151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki170Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki291Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei308PhosphoserineBy similarity1
Cross-linki317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki377Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki441Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki557Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki604Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki630Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei785PhosphoserineBy similarity1
Modified residuei798PhosphoserineBy similarity1
Cross-linki846Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki846Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki906Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki942Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki957Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1029PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8BX22.
PeptideAtlasiQ8BX22.
PRIDEiQ8BX22.

PTM databases

iPTMnetiQ8BX22.
PhosphoSitePlusiQ8BX22.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027547.
CleanExiMM_SALL4.
ExpressionAtlasiQ8BX22. baseline and differential.
GenevisibleiQ8BX22. MM.

Interactioni

Subunit structurei

Interacts with POU5F1/OCT4 (By similarity). Interacts with NANOG. Interacts with BEND3.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi221236. 57 interactors.
DIPiDIP-29926N.
IntActiQ8BX22. 44 interactors.
MINTiMINT-8394991.
STRINGi10090.ENSMUSP00000029061.

Structurei

3D structure databases

ProteinModelPortaliQ8BX22.
SMRiQ8BX22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi217 – 225Poly-Gln9

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri387 – 409C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri415 – 437C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri573 – 595C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri601 – 623C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri633 – 655C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri880 – 902C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri908 – 930C2H2-type 7PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1074. Eukaryota.
ENOG410ZE3Z. LUCA.
GeneTreeiENSGT00550000074555.
HOGENOMiHOG000231986.
HOVERGENiHBG058921.
InParanoidiQ8BX22.
KOiK19871.
OMAiGVHRTNT.
OrthoDBiEOG091G00R1.
TreeFamiTF317003.

Family and domain databases

Gene3Di2.40.155.10. 1 hit.
InterProiView protein in InterPro
IPR009017. GFP.
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 8 hits.
SUPFAMiSSF57667. SSF57667. 4 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BX22-1) [UniParc]FASTAAdd to basket
Also known as: Sall4a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD
60 70 80 90 100
EASEDSIPVK RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN
110 120 130 140 150
DSEGPVPSED FSRAALSHQL GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL
160 170 180 190 200
KTEATQPSTP QDISYLPKGK VANTNVTLQA LRGTKVAVNQ RGAEAPMAPM
210 220 230 240 250
PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH ALHSGVAGAD
260 270 280 290 300
TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV
310 320 330 340 350
PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS
360 370 380 390 400
AVTLDQSKKG KGKPQNLSAS ASVLDVKAKD EVVLGKHKCR YCPKVFGTDS
410 420 430 440 450
SLQIHLRSHT GERPYVCPIC GHRFTTKGNL KVHLQRHPEV KANPQLLAEF
460 470 480 490 500
QDKGAVSAAS HYALPVPVPA DESSLSVDAE PVPVTGTPSL GLPQKLTSGP
510 520 530 540 550
NSRDLMGGSL PNDMQPGPSP ESEAGLPLLG VGMIHNPPKA GGFQGTGAPE
560 570 580 590 600
SGSETLKLQQ LVENIDKATT DPNECLICHR VLSCQSSLKM HYRTHTGERP
610 620 630 640 650
FQCKICGRAF STKGNLKTHL GVHRTNTTVK TQHSCPICQK KFTNAVMLQQ
660 670 680 690 700
HIRMHMGGQI PNTPLPESPC DFTAPEPVAV SENGSASGVC QDDAAEGMEA
710 720 730 740 750
EEVCSQDVPS GPSTVSLPVP SAHLASPSLG FSVLASLDTQ GKGALPALAL
760 770 780 790 800
QRQSSRENSS LEGGDTGPAN DSSLLVGDQE CQSRSPDATE TMCYQAVSPA
810 820 830 840 850
NSQAGSVKSR SPEGHKAEGV ESCRVDTEGR TSLPPTFIRA QPTFVKVEVP
860 870 880 890 900
GTFVGPPSMP SGMPPLLASQ PQPRRQAKQH CCTRCGKNFS SASALQIHER
910 920 930 940 950
THTGEKPFVC NICGRAFTTK GNLKVHYMTH GANNNSARRG RKLAIENPMA
960 970 980 990 1000
ALSAEGKRAP EVFSKELLSP AVSVDPASWN QYTSVLNGGL AMKTNEISVI
1010 1020 1030 1040 1050
QSGGIPTLPV SLGASSVVSN GTISKLDGSQ TGVSMPMSGN GEKLAVPDGM
1060
AKHQFPHFLE ENKIAVS
Length:1,067
Mass (Da):113,110
Last modified:July 27, 2011 - v2
Checksum:i23B92F488AAF53E5
GO
Isoform 2 (identifier: Q8BX22-2) [UniParc]FASTAAdd to basket
Also known as: Sall4b

The sequence of this isoform differs from the canonical sequence as follows:
     386-829: Missing.

Note: No experimental confirmation available.
Show »
Length:623
Mass (Da):66,215
Checksum:i8C762461A0483EF7
GO
Isoform 3 (identifier: Q8BX22-3) [UniParc]FASTAAdd to basket
Also known as: Sall4c

The sequence of this isoform differs from the canonical sequence as follows:
     40-828: Missing.

Note: No experimental confirmation available.
Show »
Length:278
Mass (Da):29,725
Checksum:iD8DCBBFE0618EF02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti865P → H in AAR91797 (Ref. 1) Curated1
Sequence conflicti950A → V in AAR91797 (Ref. 1) Curated1
Sequence conflicti950A → V in CAD32912 (Ref. 5) Curated1
Sequence conflicti969S → Y in AAR91796 (Ref. 1) Curated1
Sequence conflicti969S → Y in AAR91798 (Ref. 1) Curated1
Sequence conflicti969S → Y in BAC33598 (PubMed:16141072).Curated1
Sequence conflicti997I → T in AAR91797 (Ref. 1) Curated1
Sequence conflicti1022T → M in AAR91797 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02168640 – 828Missing in isoform 3. 1 PublicationAdd BLAST789
Alternative sequenceiVSP_021687386 – 829Missing in isoform 2. 1 PublicationAdd BLAST444

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463371 mRNA. Translation: AAR91796.1.
AY463372 mRNA. Translation: AAR91797.1.
AY463373 mRNA. Translation: AAR91798.1.
AK049188 mRNA. Translation: BAC33598.1.
AL929248 Genomic DNA. Translation: CAM21964.1.
CH466551 Genomic DNA. Translation: EDL06559.1.
AJ488904 Genomic DNA. Translation: CAD32912.1.
CCDSiCCDS17115.1. [Q8BX22-1]
CCDS17116.1. [Q8BX22-2]
CCDS17117.1. [Q8BX22-3]
RefSeqiNP_780512.2. NM_175303.4. [Q8BX22-1]
NP_958797.2. NM_201395.3. [Q8BX22-2]
NP_958798.2. NM_201396.3. [Q8BX22-3]
UniGeneiMm.434054.
Mm.491245.

Genome annotation databases

EnsembliENSMUST00000029061; ENSMUSP00000029061; ENSMUSG00000027547. [Q8BX22-1]
ENSMUST00000075044; ENSMUSP00000074556; ENSMUSG00000027547. [Q8BX22-3]
ENSMUST00000103074; ENSMUSP00000099363; ENSMUSG00000027547. [Q8BX22-2]
GeneIDi99377.
KEGGimmu:99377.
UCSCiuc008obf.1. mouse. [Q8BX22-1]
uc008obg.1. mouse. [Q8BX22-2]
uc008obh.1. mouse. [Q8BX22-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSALL4_MOUSE
AccessioniPrimary (citable) accession number: Q8BX22
Secondary accession number(s): A2AV00
, Q6S7E8, Q6S7E9, Q7TST6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: October 25, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families