Q8BX22 (SALL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sal-like protein 4

Alternative name(s):
Zinc finger protein SALL4

Gene names

Name:

Sall4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1067 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor By similarity.
Subunit structure	Interacts with NANOG. Ref.6
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the sal C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryonic limb morphogenesis Inferred from mutant phenotype PubMed 16380715. Source: MGI inner cell mass cell proliferation Inferred from mutant phenotype PubMed 16790473. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 16380715. Source: MGI neural tube closure Inferred from mutant phenotype PubMed 16790473. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16380715. Source: MGI stem cell maintenance Inferred from mutant phenotype PubMed 20720539. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ventricular septum development Inferred from mutant phenotype PubMed 16790473. Source: MGI
Cellular_component	heterochromatin Inferred from direct assay PubMed 17295837. Source: MGI transcription factor complex Inferred by curator PubMed 16380715. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Mta2	Q9R190	3	EBI-2312582,EBI-904134
Nacc1	Q7TSZ8	4	EBI-2312582,EBI-5691985
Znf143	O70230	2	EBI-2312582,EBI-5691478

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BX22-1)

Also known as: Sall4a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BX22-2)

Also known as: Sall4b;

The sequence of this isoform differs from the canonical sequence as follows:
     386-829: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BX22-3)

Also known as: Sall4c;

The sequence of this isoform differs from the canonical sequence as follows:
     40-828: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1067	1067	Sal-like protein 4		PRO_0000261416
Regions
	Zinc finger	387 – 409	23	C2H2-type 1
	Zinc finger	415 – 437	23	C2H2-type 2
	Zinc finger	573 – 595	23	C2H2-type 3
	Zinc finger	601 – 623	23	C2H2-type 4
	Zinc finger	633 – 655	23	C2H2-type 5
	Zinc finger	880 – 902	23	C2H2-type 6
	Zinc finger	908 – 930	23	C2H2-type 7
	Compositional bias	217 – 225	9	Poly-Gln
Amino acid modifications
	Modified residue	308	1	Phosphoserine By similarity
	Modified residue	755	1	Phosphoserine By similarity
	Modified residue	785	1	Phosphoserine By similarity
	Modified residue	798	1	Phosphoserine By similarity
	Modified residue	1029	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	40 – 828	789	Missing in isoform 3.		VSP_021686
	Alternative sequence	386 – 829	444	Missing in isoform 2.		VSP_021687
Experimental info
	Sequence conflict	865	1	P → H in AAR91797. Ref.1
	Sequence conflict	950	1	A → V in AAR91797. Ref.1
	Sequence conflict	950	1	A → V in CAD32912. Ref.5
	Sequence conflict	969	1	S → Y in AAR91796. Ref.1
	Sequence conflict	969	1	S → Y in AAR91798. Ref.1
	Sequence conflict	969	1	S → Y in BAC33598. Ref.2
	Sequence conflict	997	1	I → T in AAR91797. Ref.1
	Sequence conflict	1022	1	T → M in AAR91797. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Sall4a) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 23B92F488AAF53E5	FASTA	1,067	113,110
	10 20 30 40 50 60 MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD EASEDSIPVK 70 80 90 100 110 120 RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN DSEGPVPSED FSRAALSHQL 130 140 150 160 170 180 GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL KTEATQPSTP QDISYLPKGK VANTNVTLQA 190 200 210 220 230 240 LRGTKVAVNQ RGAEAPMAPM PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH 250 260 270 280 290 300 ALHSGVAGAD TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV 310 320 330 340 350 360 PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS AVTLDQSKKG 370 380 390 400 410 420 KGKPQNLSAS ASVLDVKAKD EVVLGKHKCR YCPKVFGTDS SLQIHLRSHT GERPYVCPIC 430 440 450 460 470 480 GHRFTTKGNL KVHLQRHPEV KANPQLLAEF QDKGAVSAAS HYALPVPVPA DESSLSVDAE 490 500 510 520 530 540 PVPVTGTPSL GLPQKLTSGP NSRDLMGGSL PNDMQPGPSP ESEAGLPLLG VGMIHNPPKA 550 560 570 580 590 600 GGFQGTGAPE SGSETLKLQQ LVENIDKATT DPNECLICHR VLSCQSSLKM HYRTHTGERP 610 620 630 640 650 660 FQCKICGRAF STKGNLKTHL GVHRTNTTVK TQHSCPICQK KFTNAVMLQQ HIRMHMGGQI 670 680 690 700 710 720 PNTPLPESPC DFTAPEPVAV SENGSASGVC QDDAAEGMEA EEVCSQDVPS GPSTVSLPVP 730 740 750 760 770 780 SAHLASPSLG FSVLASLDTQ GKGALPALAL QRQSSRENSS LEGGDTGPAN DSSLLVGDQE 790 800 810 820 830 840 CQSRSPDATE TMCYQAVSPA NSQAGSVKSR SPEGHKAEGV ESCRVDTEGR TSLPPTFIRA 850 860 870 880 890 900 QPTFVKVEVP GTFVGPPSMP SGMPPLLASQ PQPRRQAKQH CCTRCGKNFS SASALQIHER 910 920 930 940 950 960 THTGEKPFVC NICGRAFTTK GNLKVHYMTH GANNNSARRG RKLAIENPMA ALSAEGKRAP 970 980 990 1000 1010 1020 EVFSKELLSP AVSVDPASWN QYTSVLNGGL AMKTNEISVI QSGGIPTLPV SLGASSVVSN 1030 1040 1050 1060 GTISKLDGSQ TGVSMPMSGN GEKLAVPDGM AKHQFPHFLE ENKIAVS
	Isoform 2 (Sall4b) [UniParc]. Checksum: 8C762461A0483EF7	FASTA	623	66,215
	10 20 30 40 50 60 MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD EASEDSIPVK 70 80 90 100 110 120 RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN DSEGPVPSED FSRAALSHQL 130 140 150 160 170 180 GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL KTEATQPSTP QDISYLPKGK VANTNVTLQA 190 200 210 220 230 240 LRGTKVAVNQ RGAEAPMAPM PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH 250 260 270 280 290 300 ALHSGVAGAD TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV 310 320 330 340 350 360 PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS AVTLDQSKKG 370 380 390 400 410 420 KGKPQNLSAS ASVLDVKAKD EVVLGRTSLP PTFIRAQPTF VKVEVPGTFV GPPSMPSGMP 430 440 450 460 470 480 PLLASQPQPR RQAKQHCCTR CGKNFSSASA LQIHERTHTG EKPFVCNICG RAFTTKGNLK 490 500 510 520 530 540 VHYMTHGANN NSARRGRKLA IENPMAALSA EGKRAPEVFS KELLSPAVSV DPASWNQYTS 550 560 570 580 590 600 VLNGGLAMKT NEISVIQSGG IPTLPVSLGA SSVVSNGTIS KLDGSQTGVS MPMSGNGEKL 610 620 AVPDGMAKHQ FPHFLEENKI AVS
	Isoform 3 (Sall4c) [UniParc]. Checksum: D8DCBBFE0618EF02	FASTA	278	29,725
	10 20 30 40 50 60 MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG RTSLPPTFIR AQPTFVKVEV 70 80 90 100 110 120 PGTFVGPPSM PSGMPPLLAS QPQPRRQAKQ HCCTRCGKNF SSASALQIHE RTHTGEKPFV 130 140 150 160 170 180 CNICGRAFTT KGNLKVHYMT HGANNNSARR GRKLAIENPM AALSAEGKRA PEVFSKELLS 190 200 210 220 230 240 PAVSVDPASW NQYTSVLNGG LAMKTNEISV IQSGGIPTLP VSLGASSVVS NGTISKLDGS 250 260 270 QTGVSMPMSG NGEKLAVPDG MAKHQFPHFL EENKIAVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the murine Okihiro syndrome gene (Sall4): sequence, expression and alternative splicing." Ma Y., Di C., Kang Q., Lai R., Theus J., Chai L. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Cloning and expression of Sall4." Kohlhase J., Kispert A., Heinrich M. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-958. Strain: 129/Sv.
[6]	"Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells." Wu Q., Chen X., Zhang J., Loh Y.-H., Low T.-Y., Zhang W., Zhang W., Sze S.-K., Lim B., Ng H.-H. J. Biol. Chem. 281:24090-24094(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NANOG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY463371 mRNA. Translation: AAR91796.1. AY463372 mRNA. Translation: AAR91797.1. AY463373 mRNA. Translation: AAR91798.1. AK049188 mRNA. Translation: BAC33598.1. AL929248 Genomic DNA. Translation: CAM21964.1. CH466551 Genomic DNA. Translation: EDL06559.1. AJ488904 Genomic DNA. Translation: CAD32912.1.
IPI	IPI00474168. IPI00475164. IPI00761180.
RefSeq	NP_780512.2. NM_175303.3. NP_958797.2. NM_201395.2. NP_958798.2. NM_201396.2.
UniGene	Mm.434054. Mm.490784.
3D structure databases
HSSP	HSSP built from PDB template 1SRK based on UniProtKB O35615.
ProteinModelPortal	Q8BX22.
SMR	Q8BX22. Positions 387-448, 572-657, 869-930.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29926N.
IntAct	Q8BX22. 42 interactions.
PTM databases
PhosphoSite	Q8BX22.
Proteomic databases
PRIDE	Q8BX22.
Protocols and materials databases
DNASU	99377.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029061; ENSMUSP00000029061; ENSMUSG00000027547. ENSMUST00000075044; ENSMUSP00000074556; ENSMUSG00000027547. ENSMUST00000103074; ENSMUSP00000099363; ENSMUSG00000027547.
GeneID	99377.
KEGG	mmu:99377.
Organism-specific databases
CTD	57167.
MGI	MGI:2139360. Sall4.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00550000074555.
HOGENOM	HOG000231986.
HOVERGEN	HBG058921.
InParanoid	A2AV00.
OMA	PHANIPS.
OrthoDB	EOG4WWRHV.
Gene expression databases
ArrayExpress	Q8BX22.
Bgee	Q8BX22.
CleanEx	MM_SALL4.
Genevestigator	Q8BX22.
GermOnline	ENSMUSG00000027547. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 7 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 8 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 7 hits. PS50157. ZINC_FINGER_C2H2_2. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	353905.
SOURCE	Search...

Entry information

Entry name

SALL4_MOUSE

Accession

Primary (citable) accession number: Q8BX22
Secondary accession number(s): A2AV00 , Q6S7E8, Q6S7E9, Q7TST6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program