Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8BX22

- SALL4_MOUSE

UniProt

Q8BX22 - SALL4_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sal-like protein 4

Gene

Sall4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri387 – 40923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri415 – 43723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri573 – 59523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri601 – 62323C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri633 – 65523C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri880 – 90223C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri908 – 93023C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. embryonic limb morphogenesis Source: MGI
  2. heart development Source: MGI
  3. inner cell mass cell proliferation Source: MGI
  4. in utero embryonic development Source: MGI
  5. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  6. neural tube closure Source: MGI
  7. neural tube development Source: MGI
  8. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  9. stem cell maintenance Source: MGI
  10. tissue development Source: MGI
  11. transcription, DNA-templated Source: UniProtKB-KW
  12. ventricular septum development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_216222. Transcriptional regulation of pluripotent stem cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Sal-like protein 4
Alternative name(s):
Zinc finger protein SALL4
Gene namesi
Name:Sall4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2139360. Sall4.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. heterochromatin Source: MGI
  3. nucleus Source: MGI
  4. protein complex Source: MGI
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10671067Sal-like protein 4PRO_0000261416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphoserineBy similarity
Cross-linki151 – 151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity
Modified residuei308 – 3081PhosphoserineBy similarity
Cross-linki317 – 317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity
Cross-linki379 – 379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity
Modified residuei755 – 7551PhosphoserineBy similarity
Modified residuei785 – 7851PhosphoserineBy similarity
Modified residuei798 – 7981PhosphoserineBy similarity
Cross-linki846 – 846Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity
Modified residuei1029 – 10291PhosphoserineBy similarity

Post-translational modificationi

Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ8BX22.

PTM databases

PhosphoSiteiQ8BX22.

Expressioni

Gene expression databases

BgeeiQ8BX22.
CleanExiMM_SALL4.
ExpressionAtlasiQ8BX22. baseline and differential.
GenevestigatoriQ8BX22.

Interactioni

Subunit structurei

Interacts with POU5F1/OCT4 (By similarity). Interacts with NANOG.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mta2Q9R1903EBI-2312582,EBI-904134
Nacc1Q7TSZ84EBI-2312582,EBI-5691985
NRBP1Q9UHY12EBI-2312582,EBI-749731From a different organism.
Znf143O702302EBI-2312582,EBI-5691478

Protein-protein interaction databases

BioGridi221236. 55 interactions.
DIPiDIP-29926N.
IntActiQ8BX22. 43 interactions.
MINTiMINT-8394991.

Structurei

3D structure databases

ProteinModelPortaliQ8BX22.
SMRiQ8BX22. Positions 389-437, 572-657, 869-930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi217 – 2259Poly-Gln

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri387 – 40923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri415 – 43723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri573 – 59523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri601 – 62323C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri633 – 65523C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri880 – 90223C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri908 – 93023C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00550000074555.
HOGENOMiHOG000231986.
HOVERGENiHBG058921.
InParanoidiQ8BX22.
OMAiPHANIPS.
OrthoDBiEOG7NCV2P.
TreeFamiTF317003.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 8 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BX22-1) [UniParc]FASTAAdd to Basket

Also known as: Sall4a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD
60 70 80 90 100
EASEDSIPVK RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN
110 120 130 140 150
DSEGPVPSED FSRAALSHQL GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL
160 170 180 190 200
KTEATQPSTP QDISYLPKGK VANTNVTLQA LRGTKVAVNQ RGAEAPMAPM
210 220 230 240 250
PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH ALHSGVAGAD
260 270 280 290 300
TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV
310 320 330 340 350
PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS
360 370 380 390 400
AVTLDQSKKG KGKPQNLSAS ASVLDVKAKD EVVLGKHKCR YCPKVFGTDS
410 420 430 440 450
SLQIHLRSHT GERPYVCPIC GHRFTTKGNL KVHLQRHPEV KANPQLLAEF
460 470 480 490 500
QDKGAVSAAS HYALPVPVPA DESSLSVDAE PVPVTGTPSL GLPQKLTSGP
510 520 530 540 550
NSRDLMGGSL PNDMQPGPSP ESEAGLPLLG VGMIHNPPKA GGFQGTGAPE
560 570 580 590 600
SGSETLKLQQ LVENIDKATT DPNECLICHR VLSCQSSLKM HYRTHTGERP
610 620 630 640 650
FQCKICGRAF STKGNLKTHL GVHRTNTTVK TQHSCPICQK KFTNAVMLQQ
660 670 680 690 700
HIRMHMGGQI PNTPLPESPC DFTAPEPVAV SENGSASGVC QDDAAEGMEA
710 720 730 740 750
EEVCSQDVPS GPSTVSLPVP SAHLASPSLG FSVLASLDTQ GKGALPALAL
760 770 780 790 800
QRQSSRENSS LEGGDTGPAN DSSLLVGDQE CQSRSPDATE TMCYQAVSPA
810 820 830 840 850
NSQAGSVKSR SPEGHKAEGV ESCRVDTEGR TSLPPTFIRA QPTFVKVEVP
860 870 880 890 900
GTFVGPPSMP SGMPPLLASQ PQPRRQAKQH CCTRCGKNFS SASALQIHER
910 920 930 940 950
THTGEKPFVC NICGRAFTTK GNLKVHYMTH GANNNSARRG RKLAIENPMA
960 970 980 990 1000
ALSAEGKRAP EVFSKELLSP AVSVDPASWN QYTSVLNGGL AMKTNEISVI
1010 1020 1030 1040 1050
QSGGIPTLPV SLGASSVVSN GTISKLDGSQ TGVSMPMSGN GEKLAVPDGM
1060
AKHQFPHFLE ENKIAVS
Length:1,067
Mass (Da):113,110
Last modified:July 27, 2011 - v2
Checksum:i23B92F488AAF53E5
GO
Isoform 2 (identifier: Q8BX22-2) [UniParc]FASTAAdd to Basket

Also known as: Sall4b

The sequence of this isoform differs from the canonical sequence as follows:
     386-829: Missing.

Note: No experimental confirmation available.

Show »
Length:623
Mass (Da):66,215
Checksum:i8C762461A0483EF7
GO
Isoform 3 (identifier: Q8BX22-3) [UniParc]FASTAAdd to Basket

Also known as: Sall4c

The sequence of this isoform differs from the canonical sequence as follows:
     40-828: Missing.

Note: No experimental confirmation available.

Show »
Length:278
Mass (Da):29,725
Checksum:iD8DCBBFE0618EF02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti865 – 8651P → H in AAR91797. 1 PublicationCurated
Sequence conflicti950 – 9501A → V in AAR91797. 1 PublicationCurated
Sequence conflicti950 – 9501A → V in CAD32912. 1 PublicationCurated
Sequence conflicti969 – 9691S → Y in AAR91796. 1 PublicationCurated
Sequence conflicti969 – 9691S → Y in AAR91798. 1 PublicationCurated
Sequence conflicti969 – 9691S → Y in BAC33598. (PubMed:16141072)Curated
Sequence conflicti997 – 9971I → T in AAR91797. 1 PublicationCurated
Sequence conflicti1022 – 10221T → M in AAR91797. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei40 – 828789Missing in isoform 3. 1 PublicationVSP_021686Add
BLAST
Alternative sequencei386 – 829444Missing in isoform 2. 1 PublicationVSP_021687Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY463371 mRNA. Translation: AAR91796.1.
AY463372 mRNA. Translation: AAR91797.1.
AY463373 mRNA. Translation: AAR91798.1.
AK049188 mRNA. Translation: BAC33598.1.
AL929248 Genomic DNA. Translation: CAM21964.1.
CH466551 Genomic DNA. Translation: EDL06559.1.
AJ488904 Genomic DNA. Translation: CAD32912.1.
CCDSiCCDS17115.1. [Q8BX22-1]
CCDS17116.1. [Q8BX22-2]
CCDS17117.1. [Q8BX22-3]
RefSeqiNP_780512.2. NM_175303.3. [Q8BX22-1]
NP_958797.2. NM_201395.2. [Q8BX22-2]
NP_958798.2. NM_201396.2. [Q8BX22-3]
UniGeneiMm.434054.
Mm.491245.

Genome annotation databases

EnsembliENSMUST00000029061; ENSMUSP00000029061; ENSMUSG00000027547. [Q8BX22-1]
ENSMUST00000075044; ENSMUSP00000074556; ENSMUSG00000027547. [Q8BX22-3]
ENSMUST00000103074; ENSMUSP00000099363; ENSMUSG00000027547. [Q8BX22-2]
GeneIDi99377.
KEGGimmu:99377.
UCSCiuc008obf.1. mouse. [Q8BX22-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY463371 mRNA. Translation: AAR91796.1 .
AY463372 mRNA. Translation: AAR91797.1 .
AY463373 mRNA. Translation: AAR91798.1 .
AK049188 mRNA. Translation: BAC33598.1 .
AL929248 Genomic DNA. Translation: CAM21964.1 .
CH466551 Genomic DNA. Translation: EDL06559.1 .
AJ488904 Genomic DNA. Translation: CAD32912.1 .
CCDSi CCDS17115.1. [Q8BX22-1 ]
CCDS17116.1. [Q8BX22-2 ]
CCDS17117.1. [Q8BX22-3 ]
RefSeqi NP_780512.2. NM_175303.3. [Q8BX22-1 ]
NP_958797.2. NM_201395.2. [Q8BX22-2 ]
NP_958798.2. NM_201396.2. [Q8BX22-3 ]
UniGenei Mm.434054.
Mm.491245.

3D structure databases

ProteinModelPortali Q8BX22.
SMRi Q8BX22. Positions 389-437, 572-657, 869-930.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 221236. 55 interactions.
DIPi DIP-29926N.
IntActi Q8BX22. 43 interactions.
MINTi MINT-8394991.

PTM databases

PhosphoSitei Q8BX22.

Proteomic databases

PRIDEi Q8BX22.

Protocols and materials databases

DNASUi 99377.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029061 ; ENSMUSP00000029061 ; ENSMUSG00000027547 . [Q8BX22-1 ]
ENSMUST00000075044 ; ENSMUSP00000074556 ; ENSMUSG00000027547 . [Q8BX22-3 ]
ENSMUST00000103074 ; ENSMUSP00000099363 ; ENSMUSG00000027547 . [Q8BX22-2 ]
GeneIDi 99377.
KEGGi mmu:99377.
UCSCi uc008obf.1. mouse. [Q8BX22-1 ]

Organism-specific databases

CTDi 57167.
MGIi MGI:2139360. Sall4.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00550000074555.
HOGENOMi HOG000231986.
HOVERGENi HBG058921.
InParanoidi Q8BX22.
OMAi PHANIPS.
OrthoDBi EOG7NCV2P.
TreeFami TF317003.

Enzyme and pathway databases

Reactomei REACT_216222. Transcriptional regulation of pluripotent stem cells.

Miscellaneous databases

NextBioi 353905.
PROi Q8BX22.
SOURCEi Search...

Gene expression databases

Bgeei Q8BX22.
CleanExi MM_SALL4.
ExpressionAtlasi Q8BX22. baseline and differential.
Genevestigatori Q8BX22.

Family and domain databases

Gene3Di 3.30.160.60. 7 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 8 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the murine Okihiro syndrome gene (Sall4): sequence, expression and alternative splicing."
    Ma Y., Di C., Kang Q., Lai R., Theus J., Chai L.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Cloning and expression of Sall4."
    Kohlhase J., Kispert A., Heinrich M.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-958.
    Strain: 129/Sv.
  6. "Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells."
    Wu Q., Chen X., Zhang J., Loh Y.-H., Low T.-Y., Zhang W., Zhang W., Sze S.-K., Lim B., Ng H.-H.
    J. Biol. Chem. 281:24090-24094(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NANOG.

Entry informationi

Entry nameiSALL4_MOUSE
AccessioniPrimary (citable) accession number: Q8BX22
Secondary accession number(s): A2AV00
, Q6S7E8, Q6S7E9, Q7TST6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3