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Q8BX22 (SALL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sal-like protein 4
Alternative name(s):
Zinc finger protein SALL4
Gene names
Name:Sall4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1067 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells By similarity.

Subunit structure

Interacts with POU5F1/OCT4 By similarity. Interacts with NANOG. Ref.6

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Post-translational modification

Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4 By similarity.

Sequence similarities

Belongs to the sal C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DiseaseOncogene
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic limb morphogenesis

Inferred from mutant phenotype PubMed 16380715. Source: MGI

heart development

Inferred from mutant phenotype PubMed 16380715. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 17060609. Source: MGI

inner cell mass cell proliferation

Inferred from mutant phenotype PubMed 16790473. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16380715. Source: MGI

neural tube closure

Inferred from mutant phenotype PubMed 16790473. Source: MGI

neural tube development

Inferred from genetic interaction PubMed 18818376. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16380715. Source: MGI

stem cell maintenance

Inferred from mutant phenotype PubMed 20720539. Source: MGI

tissue development

Inferred from mutant phenotype PubMed 17060609. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ventricular septum development

Inferred from mutant phenotype PubMed 16790473. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

heterochromatin

Inferred from direct assay PubMed 17295837. Source: MGI

nucleus

Inferred from direct assay PubMed 19796622. Source: MGI

protein complex

Inferred from direct assay PubMed 19796622. Source: MGI

transcription factor complex

Inferred by curator PubMed 16380715. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BX22-1)

Also known as: Sall4a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BX22-2)

Also known as: Sall4b;

The sequence of this isoform differs from the canonical sequence as follows:
     386-829: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8BX22-3)

Also known as: Sall4c;

The sequence of this isoform differs from the canonical sequence as follows:
     40-828: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10671067Sal-like protein 4
PRO_0000261416

Regions

Zinc finger387 – 40923C2H2-type 1
Zinc finger415 – 43723C2H2-type 2
Zinc finger573 – 59523C2H2-type 3
Zinc finger601 – 62323C2H2-type 4
Zinc finger633 – 65523C2H2-type 5
Zinc finger880 – 90223C2H2-type 6
Zinc finger908 – 93023C2H2-type 7
Compositional bias217 – 2259Poly-Gln

Amino acid modifications

Modified residue531Phosphoserine By similarity
Modified residue3081Phosphoserine By similarity
Modified residue7551Phosphoserine By similarity
Modified residue7851Phosphoserine By similarity
Modified residue7981Phosphoserine By similarity
Modified residue10291Phosphoserine By similarity
Cross-link151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity
Cross-link317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity
Cross-link379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity
Cross-link846Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Natural variations

Alternative sequence40 – 828789Missing in isoform 3.
VSP_021686
Alternative sequence386 – 829444Missing in isoform 2.
VSP_021687

Experimental info

Sequence conflict8651P → H in AAR91797. Ref.1
Sequence conflict9501A → V in AAR91797. Ref.1
Sequence conflict9501A → V in CAD32912. Ref.5
Sequence conflict9691S → Y in AAR91796. Ref.1
Sequence conflict9691S → Y in AAR91798. Ref.1
Sequence conflict9691S → Y in BAC33598. Ref.2
Sequence conflict9971I → T in AAR91797. Ref.1
Sequence conflict10221T → M in AAR91797. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Sall4a) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 23B92F488AAF53E5

FASTA1,067113,110
        10         20         30         40         50         60 
MSRRKQAKPQ HINWEEGQGE QPQQLPSPDL AEALAAEEPG APVNSPGNCD EASEDSIPVK 

        70         80         90        100        110        120 
RPRREDTHIC NKCCAEFFSL SEFMEHKKSC TKTPPVLIMN DSEGPVPSED FSRAALSHQL 

       130        140        150        160        170        180 
GSPSNKDSLQ ENGSSSGDLK KLGTDSILYL KTEATQPSTP QDISYLPKGK VANTNVTLQA 

       190        200        210        220        230        240 
LRGTKVAVNQ RGAEAPMAPM PAAQGIPWVL EQILCLQQQQ LQQIQLTEQI RVQVNMWAAH 

       250        260        270        280        290        300 
ALHSGVAGAD TLKALSSHVS QQVSVSQQVS AAVALLSQKA SNPALSLDAL KQAKLPHASV 

       310        320        330        340        350        360 
PSAASPLSSG LTSFTLKPDG TRVLPNFVSR LPSALLPQTP GSVLLQSPFS AVTLDQSKKG 

       370        380        390        400        410        420 
KGKPQNLSAS ASVLDVKAKD EVVLGKHKCR YCPKVFGTDS SLQIHLRSHT GERPYVCPIC 

       430        440        450        460        470        480 
GHRFTTKGNL KVHLQRHPEV KANPQLLAEF QDKGAVSAAS HYALPVPVPA DESSLSVDAE 

       490        500        510        520        530        540 
PVPVTGTPSL GLPQKLTSGP NSRDLMGGSL PNDMQPGPSP ESEAGLPLLG VGMIHNPPKA 

       550        560        570        580        590        600 
GGFQGTGAPE SGSETLKLQQ LVENIDKATT DPNECLICHR VLSCQSSLKM HYRTHTGERP 

       610        620        630        640        650        660 
FQCKICGRAF STKGNLKTHL GVHRTNTTVK TQHSCPICQK KFTNAVMLQQ HIRMHMGGQI 

       670        680        690        700        710        720 
PNTPLPESPC DFTAPEPVAV SENGSASGVC QDDAAEGMEA EEVCSQDVPS GPSTVSLPVP 

       730        740        750        760        770        780 
SAHLASPSLG FSVLASLDTQ GKGALPALAL QRQSSRENSS LEGGDTGPAN DSSLLVGDQE 

       790        800        810        820        830        840 
CQSRSPDATE TMCYQAVSPA NSQAGSVKSR SPEGHKAEGV ESCRVDTEGR TSLPPTFIRA 

       850        860        870        880        890        900 
QPTFVKVEVP GTFVGPPSMP SGMPPLLASQ PQPRRQAKQH CCTRCGKNFS SASALQIHER 

       910        920        930        940        950        960 
THTGEKPFVC NICGRAFTTK GNLKVHYMTH GANNNSARRG RKLAIENPMA ALSAEGKRAP 

       970        980        990       1000       1010       1020 
EVFSKELLSP AVSVDPASWN QYTSVLNGGL AMKTNEISVI QSGGIPTLPV SLGASSVVSN 

      1030       1040       1050       1060 
GTISKLDGSQ TGVSMPMSGN GEKLAVPDGM AKHQFPHFLE ENKIAVS 

« Hide

Isoform 2 (Sall4b) [UniParc].

Checksum: 8C762461A0483EF7
Show »

FASTA62366,215
Isoform 3 (Sall4c) [UniParc].

Checksum: D8DCBBFE0618EF02
Show »

FASTA27829,725

References

« Hide 'large scale' references
[1]"Characterization of the murine Okihiro syndrome gene (Sall4): sequence, expression and alternative splicing."
Ma Y., Di C., Kang Q., Lai R., Theus J., Chai L.
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Cloning and expression of Sall4."
Kohlhase J., Kispert A., Heinrich M.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-958.
Strain: 129/Sv.
[6]"Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells."
Wu Q., Chen X., Zhang J., Loh Y.-H., Low T.-Y., Zhang W., Zhang W., Sze S.-K., Lim B., Ng H.-H.
J. Biol. Chem. 281:24090-24094(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NANOG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY463371 mRNA. Translation: AAR91796.1.
AY463372 mRNA. Translation: AAR91797.1.
AY463373 mRNA. Translation: AAR91798.1.
AK049188 mRNA. Translation: BAC33598.1.
AL929248 Genomic DNA. Translation: CAM21964.1.
CH466551 Genomic DNA. Translation: EDL06559.1.
AJ488904 Genomic DNA. Translation: CAD32912.1.
RefSeqNP_780512.2. NM_175303.3.
NP_958797.2. NM_201395.2.
NP_958798.2. NM_201396.2.
UniGeneMm.434054.
Mm.491245.

3D structure databases

ProteinModelPortalQ8BX22.
SMRQ8BX22. Positions 386-683, 791-935.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid221236. 54 interactions.
DIPDIP-29926N.
IntActQ8BX22. 43 interactions.
MINTMINT-8394991.

PTM databases

PhosphoSiteQ8BX22.

Proteomic databases

PRIDEQ8BX22.

Protocols and materials databases

DNASU99377.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029061; ENSMUSP00000029061; ENSMUSG00000027547. [Q8BX22-1]
ENSMUST00000075044; ENSMUSP00000074556; ENSMUSG00000027547. [Q8BX22-3]
ENSMUST00000103074; ENSMUSP00000099363; ENSMUSG00000027547. [Q8BX22-2]
GeneID99377.
KEGGmmu:99377.
UCSCuc008obf.1. mouse. [Q8BX22-1]

Organism-specific databases

CTD57167.
MGIMGI:2139360. Sall4.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00550000074555.
HOGENOMHOG000231986.
HOVERGENHBG058921.
InParanoidA2AV00.
OMAPHANIPS.
OrthoDBEOG7NCV2P.
TreeFamTF317003.

Gene expression databases

BgeeQ8BX22.
CleanExMM_SALL4.
GenevestigatorQ8BX22.

Family and domain databases

Gene3D3.30.160.60. 7 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 8 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio353905.
PROQ8BX22.
SOURCESearch...

Entry information

Entry nameSALL4_MOUSE
AccessionPrimary (citable) accession number: Q8BX22
Secondary accession number(s): A2AV00 expand/collapse secondary AC list , Q6S7E8, Q6S7E9, Q7TST6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot