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Protein

Gem-associated protein 5

Gene

Gemin5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, Protein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-191859 snRNP Assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Gem-associated protein 5
Short name:
Gemin5
Gene namesi
Name:Gemin5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2449311 Gemin5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510051 – 1502Gem-associated protein 5Add BLAST1502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphoserineBy similarity1
Modified residuei624PhosphoserineBy similarity1
Cross-linki754Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei757PhosphoserineBy similarity1
Modified residuei770PhosphoserineBy similarity1
Modified residuei778PhosphoserineBy similarity1
Modified residuei845PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BX17
MaxQBiQ8BX17
PaxDbiQ8BX17
PeptideAtlasiQ8BX17
PRIDEiQ8BX17

PTM databases

iPTMnetiQ8BX17
PhosphoSitePlusiQ8BX17

Expressioni

Gene expression databases

BgeeiENSMUSG00000037275
CleanExiMM_GEMIN5
ExpressionAtlasiQ8BX17 baseline and differential
GenevisibleiQ8BX17 MM

Interactioni

Subunit structurei

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1, SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE. Identified in a SMN complex that contains GEMIN2/SIP1. Interacts with cytosolic DDX20/GEMIN3 and GEMIN4. Interacts with SNRNP70 and HNRNPU. Identified in a complex with 80S ribosomes; binds to the 60S large ribosomal subunit. Interacts with the ribosomal subunits RPL3 and RPL4.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with U4 snRNABy similarity1
Sitei284Interaction with U4 snRNABy similarity1
Sitei335Interaction with U4 snRNABy similarity1
Sitei359Interaction with U4 snRNABy similarity1
Sitei381Interaction with U4 snRNABy similarity1
Sitei422Interaction with U4 snRNABy similarity1
Sitei426Interaction with U4 snRNABy similarity1
Sitei470Interaction with U4 snRNABy similarity1
Sitei474Interaction with U4 snRNA and with the 7-methylguanosine cap of RNA moleculesBy similarity1
Sitei556Interaction with U4 snRNABy similarity1
Sitei579Interaction with U4 snRNABy similarity1
Sitei641Interaction with U4 snRNA and with the 7-methylguanosine cap of RNA moleculesBy similarity1
Sitei660Interaction with U4 snRNA and with the 7-methylguanosine cap of RNA moleculesBy similarity1
Sitei684Interaction with U4 snRNA and with the 7-methylguanosine cap of RNA moleculesBy similarity1

Protein-protein interaction databases

IntActiQ8BX17, 3 interactors
STRINGi10090.ENSMUSP00000131842

Structurei

3D structure databases

ProteinModelPortaliQ8BX17
SMRiQ8BX17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati62 – 104WD 1Add BLAST43
Repeati107 – 148WD 2Add BLAST42
Repeati150 – 189WD 3Add BLAST40
Repeati193 – 264WD 4Add BLAST72
Repeati280 – 321WD 5Add BLAST42
Repeati333 – 374WD 6Add BLAST42
Repeati377 – 417WD 7Add BLAST41
Repeati424 – 464WD 8Add BLAST41
Repeati468 – 509WD 9Add BLAST42
Repeati533 – 573WD 10Add BLAST41
Repeati576 – 622WD 11Add BLAST47
Repeati637 – 677WD 12Add BLAST41
Repeati680 – 720WD 13Add BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 124Important for interaction with U1 snRNABy similarityAdd BLAST124
Regioni13 – 15Interaction with U4 snRNABy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1355 – 1382Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi738 – 746Poly-Lys9

Domaini

The WD repeat domain mediates binding to U1 snRNA and to U4 snRNA. The WD repeat domain also mediates binding to the 7-methylguanosine cap that is found both on mRNA and snRNA molecules. The regions that bind snRNA molecules and the isolated 7-methylguanosine cap overlap at least partially. Besides, the WD repeat domain mediates interaction with the 60S large ribosomal subunit.By similarity

Sequence similaritiesi

Belongs to the WD repeat gemin-5 family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IJDN Eukaryota
ENOG410XTH3 LUCA
GeneTreeiENSGT00620000088064
HOVERGENiHBG051721
InParanoidiQ8BX17
KOiK13133
TreeFamiTF328886

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR020472 G-protein_beta_WD-40_rep
IPR011047 Quinoprotein_ADH-like_supfam
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 3 hits
PF00400 WD40, 2 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 13 hits
SUPFAMiSSF50978 SSF50978, 2 hits
SSF50998 SSF50998, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BX17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPEPRTLPP SPNWYCSRCS DAAPGGIFGF AARTSVFLVR VGPGAGASPG
60 70 80 90 100
APPFRVVGEL VGHTERVSGF TFSHHPGQYN LCATSSDDGT VKVWDVETKT
110 120 130 140 150
VVTEHTLHQH TISALHWSPT VKDLIVSGDE KGVVFCYWLN RNDSQHLFTE
160 170 180 190 200
PRTIFCLTCS PHHENLVAIG YKDGIVVIID ISKKGEVIHR LRGHDDEIHS
210 220 230 240 250
IAWCPLSGED CLSISQEENS EEPDIPNGKL IAETPITKGC YLATGSKDQT
260 270 280 290 300
IRIWSCSRGR GVMVLKLPFL KRRSGGVDPT VKERLWLTLH WPKNQPTQLV
310 320 330 340 350
SSCFGGELLL WDLTQSWRRK YTLFSTSAEG HNHSRIVFNL CSLKTEDGKQ
360 370 380 390 400
LLLSTSMDRD VKCWDMATLE CCWTLPSLGG FAYSLAFSPV DVGSLAIGVG
410 420 430 440 450
DGMIRVWNTL SIKNNYDVKN FWQGVKSKVT ALCWHPNKEG CLAFGTDDGK
460 470 480 490 500
VGLYDTCSNK PPQISSTYHK KTVYRLAWGP PVPPMSLGGE GDRPSLTLYS
510 520 530 540 550
CGGEGVVLQH NPWKLSGEAF DINKLVRDTN SIRYKLPVHT EISWKGDGKV
560 570 580 590 600
LALGNEDGSI EIFQVPNLRL LCTIQQHHKL VNAIVWHHEH GSRPELSCLL
610 620 630 640 650
ASGSNNAVIY VHNLKAVLES NPESPITITE PYRTLSGHTA KITSLAWSPH
660 670 680 690 700
HDGRLVSACY DGTAQVWDAL REEPLFNFRG HRGRLLCVAW SPVDPECIYS
710 720 730 740 750
GADDFCVYRW LTSMQDHSRP PQGKKCIELE KKRLSQFKPK LKKKKKPTLR
760 770 780 790 800
LPVKQDSSVG NEDESVKENS GPAENGLSDQ DGEEEAQEPE LPPSPVVCVE
810 820 830 840 850
PVSCTDICSG FEKSKVTVSS KATSLKKEPA KEKPEALLKK RKARSMLPLS
860 870 880 890 900
TSLDHRSKEE LHRDCLVLAT ATHAKAELNE DVSADLEERF HLGLFTDRAT
910 920 930 940 950
LYRMMETEGK GHLESGHPEL FHQLMLWKGD LKGVLQAAAE RGELTDSLVA
960 970 980 990 1000
VAPVAGYSVW LWAVEAFAKQ LCFQDQYVKA ASYLLSIHKV YEAVELLKSN
1010 1020 1030 1040 1050
HLYREAIAVA KARLRPEDPV LKELYLSWGS ILERDGHYAI AAKCYLGATS
1060 1070 1080 1090 1100
AYDAAKVLAR KGDAASLRTA AELAAIAGEH ELAASLALRC AQELLLMKNW
1110 1120 1130 1140 1150
VGAQEALGLH ESLQGQRLVF CLLELLCRHL EEKQPLEVRG PSSIYHQWAT
1160 1170 1180 1190 1200
GSEGTLVQRV TGVWRSAFSV DTPEQCQAAL QKLQDVKYPS ATSNTPFRQL
1210 1220 1230 1240 1250
LLHVCHDLTL AMLSQQAAAW EEAVPALLQA VVRSYTSGNF TLMQEIYSAF
1260 1270 1280 1290 1300
LPGGCDHLRD KLGDLSPAMA AYKSLEAFCI YGQLYEVWWS LCGPGPESSV
1310 1320 1330 1340 1350
WVLSAESTVS DKQSKPEDSA SAEDMEQPPG PGPRLSAESE RLLSACKELF
1360 1370 1380 1390 1400
SERHASLQTS QRTVAEVQET LAEMIRQHQK SQLCKATTNG PSRDEPSRDE
1410 1420 1430 1440 1450
PSQEAERAPS QPPSPTEERN APVSLPELTR RLTEANERIA EFPESVKAWP
1460 1470 1480 1490 1500
FPDVLECCLV LLHIGSQCPD AVDPEMQQQA QELLHKYGHT RAYRRHCQSR

HT
Length:1,502
Mass (Da):166,592
Last modified:June 28, 2011 - v2
Checksum:iB3F9889CA20E2271
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1236T → A in BAC33614 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049216 mRNA Translation: BAC33614.1
AK143536 mRNA Translation: BAE25423.1
AL672182 Genomic DNA No translation available.
CCDSiCCDS24723.1
RefSeqiNP_001160141.1, NM_001166669.1
NP_001160142.1, NM_001166670.1
NP_001160143.1, NM_001166671.1
NP_766146.2, NM_172558.3
UniGeneiMm.275349

Genome annotation databases

EnsembliENSMUST00000035604; ENSMUSP00000036603; ENSMUSG00000037275
GeneIDi216766
KEGGimmu:216766
UCSCiuc007jan.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGEMI5_MOUSE
AccessioniPrimary (citable) accession number: Q8BX17
Secondary accession number(s): Q3UPH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health