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Protein

Gem-associated protein 5

Gene

Gemin5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Gem-associated protein 5
Short name:
Gemin5
Gene namesi
Name:Gemin5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2449311. Gemin5.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15021502Gem-associated protein 5PRO_0000051005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineBy similarity
Modified residuei757 – 7571PhosphoserineBy similarity
Modified residuei770 – 7701PhosphoserineBy similarity
Modified residuei778 – 7781PhosphoserineBy similarity
Modified residuei845 – 8451PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BX17.
MaxQBiQ8BX17.
PaxDbiQ8BX17.
PRIDEiQ8BX17.

PTM databases

iPTMnetiQ8BX17.
PhosphoSiteiQ8BX17.

Expressioni

Gene expression databases

BgeeiQ8BX17.
CleanExiMM_GEMIN5.
ExpressionAtlasiQ8BX17. baseline and differential.
GenevisibleiQ8BX17. MM.

Interactioni

Subunit structurei

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1, SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BX17. 3 interactions.
STRINGi10090.ENSMUSP00000131842.

Structurei

3D structure databases

ProteinModelPortaliQ8BX17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati62 – 10443WD 1Add
BLAST
Repeati107 – 14842WD 2Add
BLAST
Repeati150 – 18940WD 3Add
BLAST
Repeati193 – 26472WD 4Add
BLAST
Repeati280 – 32142WD 5Add
BLAST
Repeati333 – 37442WD 6Add
BLAST
Repeati377 – 41741WD 7Add
BLAST
Repeati424 – 46441WD 8Add
BLAST
Repeati468 – 50942WD 9Add
BLAST
Repeati533 – 57341WD 10Add
BLAST
Repeati576 – 62247WD 11Add
BLAST
Repeati637 – 67741WD 12Add
BLAST
Repeati680 – 72041WD 13Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1355 – 138228Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi738 – 7469Poly-Lys

Sequence similaritiesi

Belongs to the WD repeat gemin-5 family.Curated
Contains 13 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IJDN. Eukaryota.
ENOG410XTH3. LUCA.
GeneTreeiENSGT00620000088064.
HOVERGENiHBG051721.
InParanoidiQ8BX17.
KOiK13133.
OrthoDBiEOG7FXZXG.
TreeFamiTF328886.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR024977. Apc4_WD40_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 3 hits.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 5 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BX17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPEPRTLPP SPNWYCSRCS DAAPGGIFGF AARTSVFLVR VGPGAGASPG
60 70 80 90 100
APPFRVVGEL VGHTERVSGF TFSHHPGQYN LCATSSDDGT VKVWDVETKT
110 120 130 140 150
VVTEHTLHQH TISALHWSPT VKDLIVSGDE KGVVFCYWLN RNDSQHLFTE
160 170 180 190 200
PRTIFCLTCS PHHENLVAIG YKDGIVVIID ISKKGEVIHR LRGHDDEIHS
210 220 230 240 250
IAWCPLSGED CLSISQEENS EEPDIPNGKL IAETPITKGC YLATGSKDQT
260 270 280 290 300
IRIWSCSRGR GVMVLKLPFL KRRSGGVDPT VKERLWLTLH WPKNQPTQLV
310 320 330 340 350
SSCFGGELLL WDLTQSWRRK YTLFSTSAEG HNHSRIVFNL CSLKTEDGKQ
360 370 380 390 400
LLLSTSMDRD VKCWDMATLE CCWTLPSLGG FAYSLAFSPV DVGSLAIGVG
410 420 430 440 450
DGMIRVWNTL SIKNNYDVKN FWQGVKSKVT ALCWHPNKEG CLAFGTDDGK
460 470 480 490 500
VGLYDTCSNK PPQISSTYHK KTVYRLAWGP PVPPMSLGGE GDRPSLTLYS
510 520 530 540 550
CGGEGVVLQH NPWKLSGEAF DINKLVRDTN SIRYKLPVHT EISWKGDGKV
560 570 580 590 600
LALGNEDGSI EIFQVPNLRL LCTIQQHHKL VNAIVWHHEH GSRPELSCLL
610 620 630 640 650
ASGSNNAVIY VHNLKAVLES NPESPITITE PYRTLSGHTA KITSLAWSPH
660 670 680 690 700
HDGRLVSACY DGTAQVWDAL REEPLFNFRG HRGRLLCVAW SPVDPECIYS
710 720 730 740 750
GADDFCVYRW LTSMQDHSRP PQGKKCIELE KKRLSQFKPK LKKKKKPTLR
760 770 780 790 800
LPVKQDSSVG NEDESVKENS GPAENGLSDQ DGEEEAQEPE LPPSPVVCVE
810 820 830 840 850
PVSCTDICSG FEKSKVTVSS KATSLKKEPA KEKPEALLKK RKARSMLPLS
860 870 880 890 900
TSLDHRSKEE LHRDCLVLAT ATHAKAELNE DVSADLEERF HLGLFTDRAT
910 920 930 940 950
LYRMMETEGK GHLESGHPEL FHQLMLWKGD LKGVLQAAAE RGELTDSLVA
960 970 980 990 1000
VAPVAGYSVW LWAVEAFAKQ LCFQDQYVKA ASYLLSIHKV YEAVELLKSN
1010 1020 1030 1040 1050
HLYREAIAVA KARLRPEDPV LKELYLSWGS ILERDGHYAI AAKCYLGATS
1060 1070 1080 1090 1100
AYDAAKVLAR KGDAASLRTA AELAAIAGEH ELAASLALRC AQELLLMKNW
1110 1120 1130 1140 1150
VGAQEALGLH ESLQGQRLVF CLLELLCRHL EEKQPLEVRG PSSIYHQWAT
1160 1170 1180 1190 1200
GSEGTLVQRV TGVWRSAFSV DTPEQCQAAL QKLQDVKYPS ATSNTPFRQL
1210 1220 1230 1240 1250
LLHVCHDLTL AMLSQQAAAW EEAVPALLQA VVRSYTSGNF TLMQEIYSAF
1260 1270 1280 1290 1300
LPGGCDHLRD KLGDLSPAMA AYKSLEAFCI YGQLYEVWWS LCGPGPESSV
1310 1320 1330 1340 1350
WVLSAESTVS DKQSKPEDSA SAEDMEQPPG PGPRLSAESE RLLSACKELF
1360 1370 1380 1390 1400
SERHASLQTS QRTVAEVQET LAEMIRQHQK SQLCKATTNG PSRDEPSRDE
1410 1420 1430 1440 1450
PSQEAERAPS QPPSPTEERN APVSLPELTR RLTEANERIA EFPESVKAWP
1460 1470 1480 1490 1500
FPDVLECCLV LLHIGSQCPD AVDPEMQQQA QELLHKYGHT RAYRRHCQSR

HT
Length:1,502
Mass (Da):166,592
Last modified:June 28, 2011 - v2
Checksum:iB3F9889CA20E2271
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1236 – 12361T → A in BAC33614 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049216 mRNA. Translation: BAC33614.1.
AK143536 mRNA. Translation: BAE25423.1.
AL672182 Genomic DNA. Translation: CAM19960.1.
CCDSiCCDS24723.1.
RefSeqiNP_001160141.1. NM_001166669.1.
NP_001160142.1. NM_001166670.1.
NP_001160143.1. NM_001166671.1.
NP_766146.2. NM_172558.3.
UniGeneiMm.275349.

Genome annotation databases

EnsembliENSMUST00000035604; ENSMUSP00000036603; ENSMUSG00000037275.
GeneIDi216766.
KEGGimmu:216766.
UCSCiuc007jan.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049216 mRNA. Translation: BAC33614.1.
AK143536 mRNA. Translation: BAE25423.1.
AL672182 Genomic DNA. Translation: CAM19960.1.
CCDSiCCDS24723.1.
RefSeqiNP_001160141.1. NM_001166669.1.
NP_001160142.1. NM_001166670.1.
NP_001160143.1. NM_001166671.1.
NP_766146.2. NM_172558.3.
UniGeneiMm.275349.

3D structure databases

ProteinModelPortaliQ8BX17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BX17. 3 interactions.
STRINGi10090.ENSMUSP00000131842.

PTM databases

iPTMnetiQ8BX17.
PhosphoSiteiQ8BX17.

Proteomic databases

EPDiQ8BX17.
MaxQBiQ8BX17.
PaxDbiQ8BX17.
PRIDEiQ8BX17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035604; ENSMUSP00000036603; ENSMUSG00000037275.
GeneIDi216766.
KEGGimmu:216766.
UCSCiuc007jan.2. mouse.

Organism-specific databases

CTDi25929.
MGIiMGI:2449311. Gemin5.

Phylogenomic databases

eggNOGiENOG410IJDN. Eukaryota.
ENOG410XTH3. LUCA.
GeneTreeiENSGT00620000088064.
HOVERGENiHBG051721.
InParanoidiQ8BX17.
KOiK13133.
OrthoDBiEOG7FXZXG.
TreeFamiTF328886.

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.

Miscellaneous databases

PROiQ8BX17.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BX17.
CleanExiMM_GEMIN5.
ExpressionAtlasiQ8BX17. baseline and differential.
GenevisibleiQ8BX17. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR024977. Apc4_WD40_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 3 hits.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 5 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Liver, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGEMI5_MOUSE
AccessioniPrimary (citable) accession number: Q8BX17
Secondary accession number(s): Q3UPH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 28, 2011
Last modified: June 8, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.