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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • necroptotic process Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Necrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:Pgam5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919792. Pgam5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 29HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002887831 – 288Serine/threonine-protein phosphatase PGAM5, mitochondrialAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79PhosphoserineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei143N6-acetyllysineBy similarity1
Modified residuei190N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BX10.
PaxDbiQ8BX10.
PeptideAtlasiQ8BX10.
PRIDEiQ8BX10.

PTM databases

iPTMnetiQ8BX10.
PhosphoSitePlusiQ8BX10.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029500.
CleanExiMM_PGAM5.
ExpressionAtlasiQ8BX10. baseline and differential.
GenevisibleiQ8BX10. MM.

Interactioni

Subunit structurei

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 (By similarity). Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation (By similarity). Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215426. 1 interactor.
DIPiDIP-32064N.
IntActiQ8BX10. 3 interactors.
STRINGi10090.ENSMUSP00000108124.

Structurei

3D structure databases

ProteinModelPortaliQ8BX10.
SMRiQ8BX10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 81Interaction with KEAP1By similarity6

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4609. Eukaryota.
ENOG410XXCQ. LUCA.
GeneTreeiENSGT00390000004796.
HOGENOMiHOG000261217.
HOVERGENiHBG105576.
InParanoidiQ8BX10.
KOiK15637.
OMAiKEDSYEI.
OrthoDBiEOG091G0DEJ.
PhylomeDBiQ8BX10.
TreeFamiTF314977.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BX10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRGGGDADTR ATEPPAWTGA
60 70 80 90 100
RAGRGVWDTN WDRREPLSLI NLKKRNVESG EDELTSRLDH YKAKATRHIF
110 120 130 140 150
LIRHSQYHVD GSLEKDRTLT PLGREQAELT GLRLASLGLK FNKIVHSSMT
160 170 180 190 200
RAVETTDIIS KHLPGVSRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG
210 220 230 240 250
ARIEAAFRNY IHRADARQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR
260 270 280
LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS
Length:288
Mass (Da):31,994
Last modified:March 1, 2003 - v1
Checksum:iB704CDF4E640F888
GO
Isoform 2 (identifier: Q8BX10-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     195-195: Missing.

Show »
Length:287
Mass (Da):31,866
Checksum:iEF709F19D24DBBD6
GO

Sequence cautioni

The sequence BAB28067 differs from that shown. Reason: Frameshift at positions 52 and 66.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46A → V in BAE26984 (PubMed:16141072).Curated1
Sequence conflicti102I → M in BAC28763 (PubMed:16141072).Curated1
Sequence conflicti144I → V in BAB28067 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025762195Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012159 mRNA. Translation: BAB28067.1. Frameshift.
AK034588 mRNA. Translation: BAC28763.1.
AK049246 mRNA. Translation: BAC33634.1.
AK146216 mRNA. Translation: BAE26984.1.
AK169643 mRNA. Translation: BAE41272.1.
BC052179 mRNA. Translation: AAH52179.1.
BC138924 mRNA. Translation: AAI38925.1.
BC138925 mRNA. Translation: AAI38926.1.
CCDSiCCDS19523.1. [Q8BX10-2]
CCDS51606.1. [Q8BX10-1]
RefSeqiNP_001157010.1. NM_001163538.1. [Q8BX10-1]
NP_082549.2. NM_028273.3. [Q8BX10-2]
UniGeneiMm.61682.

Genome annotation databases

EnsembliENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500. [Q8BX10-2]
ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500. [Q8BX10-1]
GeneIDi72542.
KEGGimmu:72542.
UCSCiuc008yqh.2. mouse. [Q8BX10-2]
uc008yqi.2. mouse. [Q8BX10-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012159 mRNA. Translation: BAB28067.1. Frameshift.
AK034588 mRNA. Translation: BAC28763.1.
AK049246 mRNA. Translation: BAC33634.1.
AK146216 mRNA. Translation: BAE26984.1.
AK169643 mRNA. Translation: BAE41272.1.
BC052179 mRNA. Translation: AAH52179.1.
BC138924 mRNA. Translation: AAI38925.1.
BC138925 mRNA. Translation: AAI38926.1.
CCDSiCCDS19523.1. [Q8BX10-2]
CCDS51606.1. [Q8BX10-1]
RefSeqiNP_001157010.1. NM_001163538.1. [Q8BX10-1]
NP_082549.2. NM_028273.3. [Q8BX10-2]
UniGeneiMm.61682.

3D structure databases

ProteinModelPortaliQ8BX10.
SMRiQ8BX10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215426. 1 interactor.
DIPiDIP-32064N.
IntActiQ8BX10. 3 interactors.
STRINGi10090.ENSMUSP00000108124.

Chemistry databases

ChEMBLiCHEMBL2331071.

PTM databases

iPTMnetiQ8BX10.
PhosphoSitePlusiQ8BX10.

Proteomic databases

EPDiQ8BX10.
PaxDbiQ8BX10.
PeptideAtlasiQ8BX10.
PRIDEiQ8BX10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500. [Q8BX10-2]
ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500. [Q8BX10-1]
GeneIDi72542.
KEGGimmu:72542.
UCSCiuc008yqh.2. mouse. [Q8BX10-2]
uc008yqi.2. mouse. [Q8BX10-1]

Organism-specific databases

CTDi192111.
MGIiMGI:1919792. Pgam5.

Phylogenomic databases

eggNOGiKOG4609. Eukaryota.
ENOG410XXCQ. LUCA.
GeneTreeiENSGT00390000004796.
HOGENOMiHOG000261217.
HOVERGENiHBG105576.
InParanoidiQ8BX10.
KOiK15637.
OMAiKEDSYEI.
OrthoDBiEOG091G0DEJ.
PhylomeDBiQ8BX10.
TreeFamiTF314977.

Miscellaneous databases

ChiTaRSiPgam5. mouse.
PROiQ8BX10.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029500.
CleanExiMM_PGAM5.
ExpressionAtlasiQ8BX10. baseline and differential.
GenevisibleiQ8BX10. MM.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGAM5_MOUSE
AccessioniPrimary (citable) accession number: Q8BX10
Secondary accession number(s): B2RSM6
, Q3UK19, Q80VY8, Q8BM78, Q9CZU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.