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Q8BX10

- PGAM5_MOUSE

UniProt

Q8BX10 - PGAM5_MOUSE

Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression By similarity. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB
    2. phosphatase activity Source: UniProtKB
    3. phosphoprotein phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB
    2. necroptotic process Source: UniProtKB
    3. positive regulation of GTPase activity Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Necrosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
    Alternative name(s):
    Phosphoglycerate mutase family member 5
    Gene namesi
    Name:Pgam5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1919792. Pgam5.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Serine/threonine-protein phosphatase PGAM5, mitochondrialPRO_0000288783Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei79 – 791PhosphoserineBy similarity
    Modified residuei115 – 1151N6-acetyllysineBy similarity
    Modified residuei143 – 1431N6-acetyllysineBy similarity
    Modified residuei190 – 1901N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BX10.
    PaxDbiQ8BX10.
    PRIDEiQ8BX10.

    PTM databases

    PhosphoSiteiQ8BX10.

    Expressioni

    Gene expression databases

    BgeeiQ8BX10.
    CleanExiMM_PGAM5.
    GenevestigatoriQ8BX10.

    Interactioni

    Subunit structurei

    Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 By similarity. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation By similarity. Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi215426. 1 interaction.
    DIPiDIP-32064N.
    IntActiQ8BX10. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BX10.
    SMRiQ8BX10. Positions 88-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 816Interaction with KEAP1By similarity

    Domaini

    The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG71348.
    GeneTreeiENSGT00390000004796.
    HOGENOMiHOG000261217.
    HOVERGENiHBG105576.
    InParanoidiQ8BX10.
    KOiK15637.
    OMAiWLRMSLN.
    OrthoDBiEOG7966H1.
    PhylomeDBiQ8BX10.
    TreeFamiTF314977.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00300. His_Phos_1. 1 hit.
    [Graphical view]
    SMARTiSM00855. PGAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BX10-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRGGGDADTR ATEPPAWTGA    50
    RAGRGVWDTN WDRREPLSLI NLKKRNVESG EDELTSRLDH YKAKATRHIF 100
    LIRHSQYHVD GSLEKDRTLT PLGREQAELT GLRLASLGLK FNKIVHSSMT 150
    RAVETTDIIS KHLPGVSRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG 200
    ARIEAAFRNY IHRADARQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR 250
    LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS 288
    Length:288
    Mass (Da):31,994
    Last modified:March 1, 2003 - v1
    Checksum:iB704CDF4E640F888
    GO
    Isoform 2 (identifier: Q8BX10-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         195-195: Missing.

    Show »
    Length:287
    Mass (Da):31,866
    Checksum:iEF709F19D24DBBD6
    GO

    Sequence cautioni

    The sequence BAB28067.1 differs from that shown. Reason: Frameshift at positions 52 and 66.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461A → V in BAE26984. (PubMed:16141072)Curated
    Sequence conflicti102 – 1021I → M in BAC28763. (PubMed:16141072)Curated
    Sequence conflicti144 – 1441I → V in BAB28067. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei195 – 1951Missing in isoform 2. 2 PublicationsVSP_025762

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012159 mRNA. Translation: BAB28067.1. Frameshift.
    AK034588 mRNA. Translation: BAC28763.1.
    AK049246 mRNA. Translation: BAC33634.1.
    AK146216 mRNA. Translation: BAE26984.1.
    AK169643 mRNA. Translation: BAE41272.1.
    BC052179 mRNA. Translation: AAH52179.1.
    BC138924 mRNA. Translation: AAI38925.1.
    BC138925 mRNA. Translation: AAI38926.1.
    CCDSiCCDS19523.1. [Q8BX10-2]
    CCDS51606.1. [Q8BX10-1]
    RefSeqiNP_001157010.1. NM_001163538.1. [Q8BX10-1]
    NP_082549.2. NM_028273.3. [Q8BX10-2]
    UniGeneiMm.61682.

    Genome annotation databases

    EnsembliENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500. [Q8BX10-2]
    ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500. [Q8BX10-1]
    GeneIDi72542.
    KEGGimmu:72542.
    UCSCiuc008yqh.2. mouse. [Q8BX10-2]
    uc008yqi.2. mouse. [Q8BX10-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012159 mRNA. Translation: BAB28067.1 . Frameshift.
    AK034588 mRNA. Translation: BAC28763.1 .
    AK049246 mRNA. Translation: BAC33634.1 .
    AK146216 mRNA. Translation: BAE26984.1 .
    AK169643 mRNA. Translation: BAE41272.1 .
    BC052179 mRNA. Translation: AAH52179.1 .
    BC138924 mRNA. Translation: AAI38925.1 .
    BC138925 mRNA. Translation: AAI38926.1 .
    CCDSi CCDS19523.1. [Q8BX10-2 ]
    CCDS51606.1. [Q8BX10-1 ]
    RefSeqi NP_001157010.1. NM_001163538.1. [Q8BX10-1 ]
    NP_082549.2. NM_028273.3. [Q8BX10-2 ]
    UniGenei Mm.61682.

    3D structure databases

    ProteinModelPortali Q8BX10.
    SMRi Q8BX10. Positions 88-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215426. 1 interaction.
    DIPi DIP-32064N.
    IntActi Q8BX10. 3 interactions.

    Chemistry

    ChEMBLi CHEMBL2331071.

    PTM databases

    PhosphoSitei Q8BX10.

    Proteomic databases

    MaxQBi Q8BX10.
    PaxDbi Q8BX10.
    PRIDEi Q8BX10.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000059229 ; ENSMUSP00000057760 ; ENSMUSG00000029500 . [Q8BX10-2 ]
    ENSMUST00000112505 ; ENSMUSP00000108124 ; ENSMUSG00000029500 . [Q8BX10-1 ]
    GeneIDi 72542.
    KEGGi mmu:72542.
    UCSCi uc008yqh.2. mouse. [Q8BX10-2 ]
    uc008yqi.2. mouse. [Q8BX10-1 ]

    Organism-specific databases

    CTDi 192111.
    MGIi MGI:1919792. Pgam5.

    Phylogenomic databases

    eggNOGi NOG71348.
    GeneTreei ENSGT00390000004796.
    HOGENOMi HOG000261217.
    HOVERGENi HBG105576.
    InParanoidi Q8BX10.
    KOi K15637.
    OMAi WLRMSLN.
    OrthoDBi EOG7966H1.
    PhylomeDBi Q8BX10.
    TreeFami TF314977.

    Miscellaneous databases

    ChiTaRSi Pgam5. mouse.
    NextBioi 336451.
    PROi Q8BX10.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BX10.
    CleanExi MM_PGAM5.
    Genevestigatori Q8BX10.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00300. His_Phos_1. 1 hit.
    [Graphical view ]
    SMARTi SM00855. PGAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J, DBA/2 and NOD.
      Tissue: Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-288 (ISOFORM 1).
      Tissue: Brain and Embryo.
    3. "The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways."
      Wang Z., Jiang H., Chen S., Du F., Wang X.
      Cell 148:228-243(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPGAM5_MOUSE
    AccessioniPrimary (citable) accession number: Q8BX10
    Secondary accession number(s): B2RSM6
    , Q3UK19, Q80VY8, Q8BM78, Q9CZU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3