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Q8BX10 (PGAM5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial

EC=3.1.3.16
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene names
Name:Pgam5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression By similarity. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. Ref.3

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subunit structure

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 By similarity. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation By similarity. Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein By similarity.

Domain

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane By similarity.

Post-translational modification

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.

Sequence similarities

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Sequence caution

The sequence BAB28067.1 differs from that shown. Reason: Frameshift at positions 52 and 66.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BX10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BX10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     195-195: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Serine/threonine-protein phosphatase PGAM5, mitochondrial
PRO_0000288783

Regions

Transmembrane7 – 2923Helical; Potential
Region76 – 816Interaction with KEAP1 By similarity

Amino acid modifications

Modified residue791Phosphoserine By similarity
Modified residue1151N6-acetyllysine By similarity
Modified residue1431N6-acetyllysine By similarity
Modified residue1901N6-acetyllysine By similarity

Natural variations

Alternative sequence1951Missing in isoform 2.
VSP_025762

Experimental info

Sequence conflict461A → V in BAE26984. Ref.1
Sequence conflict1021I → M in BAC28763. Ref.1
Sequence conflict1441I → V in BAB28067. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: B704CDF4E640F888

FASTA28831,994
        10         20         30         40         50         60 
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRGGGDADTR ATEPPAWTGA RAGRGVWDTN 

        70         80         90        100        110        120 
WDRREPLSLI NLKKRNVESG EDELTSRLDH YKAKATRHIF LIRHSQYHVD GSLEKDRTLT 

       130        140        150        160        170        180 
PLGREQAELT GLRLASLGLK FNKIVHSSMT RAVETTDIIS KHLPGVSRVS TDLLREGAPI 

       190        200        210        220        230        240 
EPDPPVSHWK PEAVQYYEDG ARIEAAFRNY IHRADARQEE DSYEIFICHA NVIRYIVCRA 

       250        260        270        280 
LQFPPEGWLR LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS 

« Hide

Isoform 2 [UniParc].

Checksum: EF709F19D24DBBD6
Show »

FASTA28731,866

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J, DBA/2 and NOD.
Tissue: Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-288 (ISOFORM 1).
Tissue: Brain and Embryo.
[3]"The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways."
Wang Z., Jiang H., Chen S., Du F., Wang X.
Cell 148:228-243(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK012159 mRNA. Translation: BAB28067.1. Frameshift.
AK034588 mRNA. Translation: BAC28763.1.
AK049246 mRNA. Translation: BAC33634.1.
AK146216 mRNA. Translation: BAE26984.1.
AK169643 mRNA. Translation: BAE41272.1.
BC052179 mRNA. Translation: AAH52179.1.
BC138924 mRNA. Translation: AAI38925.1.
BC138925 mRNA. Translation: AAI38926.1.
RefSeqNP_001157010.1. NM_001163538.1.
NP_082549.2. NM_028273.3.
UniGeneMm.61682.

3D structure databases

ProteinModelPortalQ8BX10.
SMRQ8BX10. Positions 88-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215426. 1 interaction.
IntActQ8BX10. 3 interactions.

Chemistry

ChEMBLCHEMBL2331071.

PTM databases

PhosphoSiteQ8BX10.

Proteomic databases

PaxDbQ8BX10.
PRIDEQ8BX10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000059229; ENSMUSP00000057760; ENSMUSG00000029500. [Q8BX10-2]
ENSMUST00000112505; ENSMUSP00000108124; ENSMUSG00000029500. [Q8BX10-1]
GeneID72542.
KEGGmmu:72542.
UCSCuc008yqh.2. mouse. [Q8BX10-2]
uc008yqi.2. mouse. [Q8BX10-1]

Organism-specific databases

CTD192111.
MGIMGI:1919792. Pgam5.

Phylogenomic databases

eggNOGNOG71348.
GeneTreeENSGT00390000004796.
HOGENOMHOG000261217.
HOVERGENHBG105576.
InParanoidQ8BX10.
KOK15637.
OMAWLRMSLN.
OrthoDBEOG7966H1.
PhylomeDBQ8BX10.
TreeFamTF314977.

Gene expression databases

BgeeQ8BX10.
CleanExMM_PGAM5.
GenevestigatorQ8BX10.

Family and domain databases

InterProIPR013078. His_Pase_superF_clade-1.
[Graphical view]
PfamPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTSM00855. PGAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPgam5. mouse.
NextBio336451.
PROQ8BX10.
SOURCESearch...

Entry information

Entry namePGAM5_MOUSE
AccessionPrimary (citable) accession number: Q8BX10
Secondary accession number(s): B2RSM6 expand/collapse secondary AC list , Q3UK19, Q80VY8, Q8BM78, Q9CZU2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot