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Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Gene

Ctdsp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1064-aspartylphosphate intermediateBy similarity1
Metal bindingi106MagnesiumBy similarity1
Active sitei108Proton donorBy similarity1
Metal bindingi108Magnesium; via carbonyl oxygenBy similarity1
Sitei162Transition state stabilizerBy similarity1
Sitei200Transition state stabilizerBy similarity1
Metal bindingi217MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • protein dephosphorylation Source: MGI

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 (EC:3.1.3.16)
Alternative name(s):
Small C-terminal domain phosphatase 2
Small CTD phosphatase 2
Short name:
SCP2
Gene namesi
Name:Ctdsp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1098748. Ctdsp2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125751 – 270Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BX07.
PaxDbiQ8BX07.
PRIDEiQ8BX07.

PTM databases

PhosphoSitePlusiQ8BX07.
SwissPalmiQ8BX07.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000078429.
ExpressionAtlasiQ8BX07. baseline and differential.
GenevisibleiQ8BX07. MM.

Interactioni

Subunit structurei

Monomer. Interacts with REST.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100891.

Structurei

3D structure databases

ProteinModelPortaliQ8BX07.
SMRiQ8BX07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 254FCP1 homologyPROSITE-ProRule annotationAdd BLAST159

Phylogenomic databases

eggNOGiKOG1605. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00390000017194.
HOVERGENiHBG053298.
InParanoidiQ8BX07.
KOiK15731.
OMAiCNIFKAL.
OrthoDBiEOG091G0J49.
PhylomeDBiQ8BX07.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
PfamiView protein in Pfam
PF03031. NIF. 1 hit.
SMARTiView protein in SMART
SM00577. CPDc. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiView protein in PROSITE
PS50969. FCP1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BX07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHGSIITQA RREDALVLTK QGLVSKSSPK KPRGRSIFKA LLCCFHTQHV
60 70 80 90 100
VQSSSSTELT HKEEANTIAK SDLLQCLQYQ FYQIPGTCLL PEVTEQDQGR
110 120 130 140 150
ICVVIDLDET LVHSSFKPIN NADFIVPVEI EGTTHQVYVL KRPYVDEFLR
160 170 180 190 200
RMGELFECVL FTASLAKYAD PVTDLLDRCG VFRARLFREA CVFHQGCYVK
210 220 230 240 250
DLSRLGRDLR KTVILDNSPA SYIFHPENAV PVQSWFDDMA DTELLNLIPV
260 270
FEELSGTDDV YTSLGQLRAP
Length:270
Mass (Da):30,546
Last modified:March 1, 2003 - v1
Checksum:i0098B97E0AEC1B1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049271 mRNA. Translation: BAC33649.1.
BC085142 mRNA. Translation: AAH85142.1.
CCDSiCCDS48711.1.
RefSeqiNP_001106941.1. NM_001113470.1.
NP_666124.1. NM_146012.2.
UniGeneiMm.29490.

Genome annotation databases

EnsembliENSMUST00000105256; ENSMUSP00000100891; ENSMUSG00000078429.
GeneIDi52468.
KEGGimmu:52468.
UCSCiuc007hhj.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCTDS2_MOUSE
AccessioniPrimary (citable) accession number: Q8BX07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 1, 2003
Last modified: June 7, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot