Q8BX05 (GLPK5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glycerol kinase 5
Short name=GK 5
Short name=Glycerokinase 5
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase 5

Gene names

Name:

Gk5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Sequence similarities	Belongs to the FGGY kinase family.
Sequence caution	The sequence AAH50033.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycerol metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BX05-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BX05-2)

The sequence of this isoform differs from the canonical sequence as follows:
     515-534: VNMENWVKAVKRSMNWYNKT → AF

Isoform 3 (identifier: Q8BX05-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     515-534: VNMENWVKAVKRSMNWYNKT → AF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Putative glycerol kinase 5		PRO_0000323755
Regions
	Nucleotide binding	444 – 449	6	ATP By similarity
Sites
	Binding site	33	1	Substrate By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	280	1	Substrate By similarity
	Binding site	302	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 90	90	Missing in isoform 3.		VSP_032124
	Alternative sequence	515 – 534	20	VNMEN…WYNKT → AF in isoform 2 and isoform 3.		VSP_032125
Experimental info
	Sequence conflict	45	1	A → P in AAH50033. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: E643A4284E3377FE	FASTA	534	59,812
	10 20 30 40 50 60 MSGQQERAER QREELSASAS PPSRFVLGLD VGSTVIRCHV YDQTARVRGS SAQKVENVYP 70 80 90 100 110 120 QPGWVEIDPD SLWAQFVAVI KDAVKAAGVQ MNQIVGLGIS TQRATFITWN KKTGHHFHNF 130 140 150 160 170 180 ISWQDLRAAE LVKSWNNSLI MKLLHGATRV LHFFSRSKVM LTVSRFNFST QHATLRLTWI 190 200 210 220 230 240 LQNLSEVKRA VEEDNCCFGT IDTWLLYKLT KGSSYATDYS NASTTGFFDP YAMRWSRLIT 250 260 270 280 290 300 TMVSIPLSIL PPVKDTSYNF GSVDEKIFGV PIPVVALVGD QQSAMFGECC FETGDVKLTM 310 320 330 340 350 360 GTGTFLDINT GKNLQHVNGG FYPLIGWKIG QELVCLAEGN AGDTGTAIMW AQKLDLFTDA 370 380 390 400 410 420 AETEKMALSL EDSEGVYFVP SFSGLQAPLN DPCACASFMG LKHSTNKYHL VRAILESIAF 430 440 450 460 470 480 RNKQLYDMLQ REIQIPVTNI RADGGVCNNA FVMQMTSDLI NEKIDRPAHF DMSCLGAASL 490 500 510 520 530 AGLAVGFWAD KEELQKLRQS EMVFKPQKKW QEYEVNMENW VKAVKRSMNW YNKT
	Isoform 2 [UniParc]. Checksum: 7DD0C57D3BCAB161	FASTA	516	57,550
	10 20 30 40 50 60 MSGQQERAER QREELSASAS PPSRFVLGLD VGSTVIRCHV YDQTARVRGS SAQKVENVYP 70 80 90 100 110 120 QPGWVEIDPD SLWAQFVAVI KDAVKAAGVQ MNQIVGLGIS TQRATFITWN KKTGHHFHNF 130 140 150 160 170 180 ISWQDLRAAE LVKSWNNSLI MKLLHGATRV LHFFSRSKVM LTVSRFNFST QHATLRLTWI 190 200 210 220 230 240 LQNLSEVKRA VEEDNCCFGT IDTWLLYKLT KGSSYATDYS NASTTGFFDP YAMRWSRLIT 250 260 270 280 290 300 TMVSIPLSIL PPVKDTSYNF GSVDEKIFGV PIPVVALVGD QQSAMFGECC FETGDVKLTM 310 320 330 340 350 360 GTGTFLDINT GKNLQHVNGG FYPLIGWKIG QELVCLAEGN AGDTGTAIMW AQKLDLFTDA 370 380 390 400 410 420 AETEKMALSL EDSEGVYFVP SFSGLQAPLN DPCACASFMG LKHSTNKYHL VRAILESIAF 430 440 450 460 470 480 RNKQLYDMLQ REIQIPVTNI RADGGVCNNA FVMQMTSDLI NEKIDRPAHF DMSCLGAASL 490 500 510 AGLAVGFWAD KEELQKLRQS EMVFKPQKKW QEYEAF
	Isoform 3 [UniParc]. Checksum: FBFA5C7F965D802C	FASTA	426	47,712
	10 20 30 40 50 60 MNQIVGLGIS TQRATFITWN KKTGHHFHNF ISWQDLRAAE LVKSWNNSLI MKLLHGATRV 70 80 90 100 110 120 LHFFSRSKVM LTVSRFNFST QHATLRLTWI LQNLSEVKRA VEEDNCCFGT IDTWLLYKLT 130 140 150 160 170 180 KGSSYATDYS NASTTGFFDP YAMRWSRLIT TMVSIPLSIL PPVKDTSYNF GSVDEKIFGV 190 200 210 220 230 240 PIPVVALVGD QQSAMFGECC FETGDVKLTM GTGTFLDINT GKNLQHVNGG FYPLIGWKIG 250 260 270 280 290 300 QELVCLAEGN AGDTGTAIMW AQKLDLFTDA AETEKMALSL EDSEGVYFVP SFSGLQAPLN 310 320 330 340 350 360 DPCACASFMG LKHSTNKYHL VRAILESIAF RNKQLYDMLQ REIQIPVTNI RADGGVCNNA 370 380 390 400 410 420 FVMQMTSDLI NEKIDRPAHF DMSCLGAASL AGLAVGFWAD KEELQKLRQS EMVFKPQKKW QEYEAF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Blastocyst, Egg and Stomach.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: NMRI. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK049275 mRNA. Translation: BAC33651.1. AK160477 mRNA. Translation: BAE35811.1. AK162119 mRNA. Translation: BAE36735.1. AK167217 mRNA. Translation: BAE39343.1. BC050033 mRNA. Translation: AAH50033.1. Different initiation. BC116302 mRNA. Translation: AAI16303.1. BC116303 mRNA. Translation: AAI16304.1.
IPI	IPI00475236. IPI00876178. IPI00889245.
RefSeq	NP_796326.1. NM_177352.4.
UniGene	Mm.213651.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P0A6F3.
ProteinModelPortal	Q8BX05.
SMR	Q8BX05. Positions 22-533.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BX05.
PTM databases
PhosphoSite	Q8BX05.
2D gel databases
REPRODUCTION-2DPAGE	IPI00475236.
Proteomic databases
PRIDE	Q8BX05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000085217; ENSMUSP00000082313; ENSMUSG00000041440. ENSMUST00000122383; ENSMUSP00000112717; ENSMUSG00000041440.
GeneID	235533.
KEGG	mmu:235533.
UCSC	uc009rbz.1. mouse. uc009rca.1. mouse. uc012gyo.1. mouse.
Organism-specific databases
CTD	256356.
MGI	MGI:2443336. Gk5.
Phylogenomic databases
eggNOG	roNOG11420.
GeneTree	ENSGT00530000063143.
HOGENOM	HBG511469.
HOVERGEN	HBG097793.
InParanoid	Q8BX05.
OMA	NSFVMQM.
PhylomeDB	Q8BX05.
Gene expression databases
ArrayExpress	Q8BX05.
Bgee	Q8BX05.
CleanEx	MM_GK5.
Genevestigator	Q8BX05.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

GLPK5_MOUSE

Accession

Primary (citable) accession number: Q8BX05
Secondary accession number(s): Q14B67 , Q3TK02, Q3TSE0, Q80VA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 16, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program