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Protein

Eukaryotic peptide chain release factor subunit 1

Gene

Etf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Nonsense-mediated mRNA decay, Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72764 Eukaryotic Translation Termination
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1
Short name:
Eukaryotic release factor 1
Short name:
eRF1
Gene namesi
Name:Etf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2385071 Etf1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001431402 – 437Eukaryotic peptide chain release factor subunit 1Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei347PhosphothreonineBy similarity1
Cross-linki404Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BWY3
MaxQBiQ8BWY3
PaxDbiQ8BWY3
PeptideAtlasiQ8BWY3
PRIDEiQ8BWY3

PTM databases

iPTMnetiQ8BWY3
PhosphoSitePlusiQ8BWY3
SwissPalmiQ8BWY3

Expressioni

Gene expression databases

BgeeiENSMUSG00000024360
CleanExiMM_ETF1
ExpressionAtlasiQ8BWY3 baseline and differential
GenevisibleiQ8BWY3 MM

Interactioni

Subunit structurei

Heterodimer of two subunits, one of which binds GTP. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (By similarity). Interacts with Moloney murine leukemia virus (MoLV) reverse transcriptase/Ribonuclease H p80 (via RT and RNase domains); this interaction is essential for translational readthrough of amber codon between viral gag and pol genes. Interacts with MoLV Gag-Pol precursor.By similarity1 Publication

Protein-protein interaction databases

BioGridi230388, 4 interactors
IntActiQ8BWY3, 2 interactors
STRINGi10090.ENSMUSP00000025218

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi283 – 295Combined sources13
Beta strandi301 – 304Combined sources4
Helixi305 – 313Combined sources9
Beta strandi317 – 323Combined sources7
Beta strandi329 – 334Combined sources6
Beta strandi336 – 339Combined sources4
Beta strandi341 – 346Combined sources6
Beta strandi368 – 373Combined sources6
Helixi374 – 381Combined sources8
Helixi382 – 384Combined sources3
Beta strandi388 – 392Combined sources5
Beta strandi394 – 396Combined sources3
Helixi397 – 406Combined sources10
Beta strandi409 – 415Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DMQX-ray4.00B1-437[»]
5DMRX-ray2.80B276-437[»]
ProteinModelPortaliQ8BWY3
SMRiQ8BWY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

eggNOGiKOG0688 Eukaryota
COG1503 LUCA
GeneTreeiENSGT00390000009004
HOGENOMiHOG000224681
HOVERGENiHBG005602
InParanoidiQ8BWY3
KOiK03265
OMAiGPGTEKM
OrthoDBiEOG091G0MEJ
PhylomeDBiQ8BWY3
TreeFamiTF105672

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.30.960.10, 1 hit
InterProiView protein in InterPro
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N
PANTHERiPTHR10113 PTHR10113, 1 hit
PfamiView protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194 eRF1_1, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit
TIGRFAMsiTIGR03676 aRF1/eRF1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BWY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK
60 70 80 90 100
MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI
110 120 130 140 150
VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDDSKFGFIV
160 170 180 190 200
IDGSGALFGT LQGNTREVLH KFTVDLPKKH GRGGQSALRF ARLRMEKRHN
210 220 230 240 250
YVRKVAETAV QLFISGDKVN VAGLVLAGSA DFKTELSQSD MFDQRLQSKV
260 270 280 290 300
LKLVDISYGG ENGFNQAIEL STEVLSNVKF IQEKKLIGRY FDEISQDTGK
310 320 330 340 350
YCFGVEDTLK ALEMGAVEIL IVYENLDIMR YVLHCQGTEE EKILYLTPEQ
360 370 380 390 400
EKDKSHFTDK ETGQEHELIE SMPLLEWFAN NYKKFGATLE IVTDKSQEGS
410 420 430
QFVKGFGGIG GILRYRVDFQ GMEYQGGDDE FFDLDDY
Length:437
Mass (Da):49,031
Last modified:January 23, 2007 - v4
Checksum:iCECC50D100E59D19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40P → Q in AAH13717 (PubMed:15489334).Curated1
Sequence conflicti207E → K in BAC33839 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049607 mRNA Translation: BAC33839.1
AK164020 mRNA Translation: BAE37589.1
BC013717 mRNA Translation: AAH13717.1
CCDSiCCDS29137.1
RefSeqiNP_659115.3, NM_144866.3
UniGeneiMm.329353

Genome annotation databases

EnsembliENSMUST00000025218; ENSMUSP00000025218; ENSMUSG00000024360
GeneIDi225363
KEGGimmu:225363
UCSCiuc008elu.1 mouse

Entry informationi

Entry nameiERF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWY3
Secondary accession number(s): Q3TPZ6, Q91VH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 131 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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