Pkn2

Functionⁱ

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (By similarity).By similarity3 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationⁱ

Kinase activity is activated upon binding to GTP-bound Rho1/Rac1 GTPases. Activated by caspase-3 (CASP3) cleavage during apoptosis. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-815 (activation loop of the kinase domain) and Thr-957 (turn motif), need to be phosphorylated for its full activation (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	685	ATPPROSITE-ProRule annotation		1
Active siteⁱ	781	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	662 – 670	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
cadherin binding Source: MGI
GTP-Rho binding Source: InterPro
histone deacetylase binding Source: UniProtKB
kinase activity Source: MGI
protein kinase C activity Source: UniProtKB-EC
protein serine/threonine kinase activity Source: UniProtKB
RNA polymerase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apical junction assembly
Source: UniProtKB
Inferred from direct assayⁱ
- 20974804
apoptotic process Source: UniProtKB-KW
cell adhesion Source: UniProtKB-KW
cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
cell projection organization Source: UniProtKB-KW
epithelial cell migration Source: UniProtKB
intracellular signal transduction Source: GO_Central
peptidyl-serine phosphorylation Source: GO_Central
positive regulation of cytokinesis Source: UniProtKB
positive regulation of mitotic cell cycle Source: UniProtKB
positive regulation of viral genome replication Source: MGI
protein phosphorylation Source: UniProtKB
regulation of cell motility Source: UniProtKB
regulation of transcription, DNA-templated Source: UniProtKB-KW
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis, Cell adhesion, Cell cycle, Cell division, Cilium biogenesis/degradation, Transcription, Transcription regulation
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-5625740. RHO GTPases activate PKNs.

Reactomeⁱ

R-MMU-5625740. RHO GTPases activate PKNs.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase N2 (EC:2.7.11.13) Alternative name(s): PKN gamma Protein kinase C-like 2 Protein-kinase C-related kinase 2
Gene namesⁱ	Name:Pkn2 Synonyms:Prk2, Prkcl2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:109211. Pkn2.

Keywords - Cellular componentⁱ

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	66	A → K: Inhibits interaction with RHOA, reduces localization at junctions and prevents apical junction formation; when associated with K-155. 1 Publication	1
Mutagenesisⁱ	155	A → K: Inhibits interaction with RHOA, reduces localization at junctions and prevents apical junction formation; when associated with K-60. 1 Publication	1
Mutagenesisⁱ	685	K → M: Prevents apical junction formation. 1 Publication	1
Mutagenesisⁱ	781	D → A: Prevents apical junction formation. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000055723	1 – 983	Serine/threonine-protein kinase N2Add BLAST		983

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	77	N6-acetyllysineCombined sources	1
Modified residueⁱ	110	PhosphoserineBy similarity	1
Modified residueⁱ	121	PhosphothreonineBy similarity	1
Modified residueⁱ	124	PhosphothreonineBy similarity	1
Modified residueⁱ	301	PhosphoserineBy similarity	1
Modified residueⁱ	305	PhosphoserineBy similarity	1
Modified residueⁱ	359	PhosphoserineBy similarity	1
Modified residueⁱ	361	PhosphoserineBy similarity	1
Modified residueⁱ	534	PhosphoserineBy similarity	1
Modified residueⁱ	582	PhosphoserineBy similarity	1
Modified residueⁱ	619	PhosphoserineCombined sources	1
Modified residueⁱ	630	PhosphoserineBy similarity	1
Modified residueⁱ	815	Phosphothreonine; by PDPK1By similarity	1
Modified residueⁱ	951	PhosphoserineBy similarity	1
Modified residueⁱ	957	PhosphothreonineCombined sources	1

Post-translational modificationⁱ

Phosphorylated during mitosis (By similarity). Autophosphorylated. Phosphorylated. Phosphorylated by CDK10 (By similarity).By similarity

Activated by limited proteolysis with trypsin. Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	117 – 118	Cleavage; by caspase-3By similarity		2
Siteⁱ	699 – 700	Cleavage; by caspase-3By similarity		2

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q8BWW9.
PaxDbⁱ	Q8BWW9.
PeptideAtlas More...PeptideAtlasⁱ	Q8BWW9.
PRIDEⁱ	Q8BWW9.

PTM databases

iPTMnetⁱ	Q8BWW9.
PhosphoSitePlusⁱ	Q8BWW9.

Expressionⁱ

Tissue specificityⁱ

Ubiquitous. Highly expressed in liver and lung Expressed in astrocytes (at protein level). Ubiquitous.4 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000004591.
CleanExⁱ	MM_PKN2.
ExpressionAtlasⁱ	Q8BWW9. baseline and differential.
Genevisibleⁱ	Q8BWW9. MM.

Interactionⁱ

Subunit structureⁱ

Interacts (via the REM repeats) with RHOA (GTP-bound form preferentially) and interacts (via the REM repeats) with RAC1 (GTP-bound form preferentially); the interactions induce its autophosphorylation (PubMed:17403031, PubMed:20974804). Interacts with NCK1 (via SH3 domains) (PubMed:8910519). Interacts with RHOC. Interacts with NCK1 and NCK2 (By similarity). Interacts with CD44 (PubMed:17403031). Interacts (via C-terminal kinase domain) with PDPK1; the interaction stimulates PDPK1 kinase activity (By similarity). Interacts with MAP3K2; the interaction activates PRK2 kinase activity in a MAP3K2-independent kinase activity (PubMed:10818102). Interacts (via C-terminal domain) with AKT1; the interaction occurs with the C-terminal cleavage product of PRK2 in apoptotic cells. Interacts (via C-terminus) with PTPN13 (via PDZ 3 domain). Interacts with CDK10 (By similarity).By similarity4 Publications

GO - Molecular functionⁱ

cadherin binding Source: MGI
GTP-Rho binding Source: InterPro
histone deacetylase binding Source: UniProtKB
RNA polymerase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	224651. 2 interactors.
STRINGⁱ	10090.ENSMUSP00000039566.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8BWW9.
SMRⁱ	Q8BWW9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	44 – 119	REM 1Add BLAST	76
Repeatⁱ	133 – 213	REM 2Add BLAST	81
Repeatⁱ	214 – 295	REM 3Add BLAST	82
Domainⁱ	329 – 462	C2Add BLAST	134
Domainⁱ	656 – 915	Protein kinasePROSITE-ProRule annotationAdd BLAST	260
Domainⁱ	916 – 983	AGC-kinase C-terminalAdd BLAST	68

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	381 – 462	Necessary to rescue apical junction formationAdd BLAST	82
Regionⁱ	916 – 976	Necessary for the catalytic activityBy similarityAdd BLAST	61
Regionⁱ	977 – 983	Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoABy similarity	7

Domainⁱ

The N-terminal regioninterferes with the interaction between AKT1 and the C-terminal regionof PKN2.By similarity

The C1 domain does not bind the diacylglycerol (DAG).

The apoptotic C-terminal cleavage product inhibits EGF-induced kinase activity of AKT1 phosphorylation at 'Thr-308' and 'Ser-473' sites, PDPK1 autophosphorylation and kinases PRKCD and PRKCZ phosphorylations.By similarity

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0694. Eukaryota. ENOG410XNPH. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00820000126964.
HOGENOMⁱ	HOG000233032.
HOVERGENⁱ	HBG108317.
InParanoidⁱ	Q8BWW9.
KEGG Orthology (KO) More...KOⁱ	K06071.
OMAⁱ	RSQQMFQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G00YT.
TreeFamⁱ	TF102005.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd08687. C2_PKN-like. 1 hit. cd11622. HR1_PKN_1. 1 hit.
InterProⁱ	View protein in InterPro IPR000961. AGC-kinase_C. IPR000008. C2_dom. IPR037784. C2_PKN. IPR011072. HR1_rho-bd. IPR036274. HR1_rpt_sf. IPR011009. Kinase-like_dom_sf. IPR017892. Pkinase_C. IPR037313. PKN_HR1_1. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit.
SMARTⁱ	View protein in SMART SM00239. C2. 1 hit. SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF46585. SSF46585. 3 hits. SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (3)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q8BWW9-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDFSDTM VQQKLDDIKD 
        60         70         80         90        100
RIKREIRKEL KIKEGAENLR KVTTDKKNLA YVDNILKKSN KKLEELHHKL 
       110        120        130        140        150
QELNAHIVVS DPEDSTDCPR TPDTPNSDSR SSTSNNRLMA LQKQLDIELK 
       160        170        180        190        200
VKQGAENMIQ MYSNGSSKDR KLHGTAQQLL QDSKTKIEVI RMQILQAVQT 
       210        220        230        240        250
NELAFDNAKP VISPLELRME ELRHHFKIEF AVAEGAKNVM KLLGSGKVTD 
       260        270        280        290        300
RKALSEAQAR FNESSQKLDL LKYSLEQRLN ELPRNHPKSS VVIEELSLVA 
       310        320        330        340        350
SPTLSPRQSM LSTQNQYSTL SKPAALTGTL EVRLMGCQDI LENVPGRSKA 
       360        370        380        390        400
TSVALPGWSP SDNRSSFMSR TSKSKSGSSR NLLKTDDLSN DVCAVLKLDN 
       410        420        430        440        450
TVVGQTSWKP ISNQSWDQKF TLELDRSREL EISVYWRDWR SLCAVKFLRL 
       460        470        480        490        500
EDFLDNQRHG MCLYLEPQGT LFAEVTFFNP VIERRPKLQR QKKIFSKQQG 
       510        520        530        540        550
KTFLRAPQMN INIATWGRLV RRAIPTVNHS GTFSPQTPVP ATVPVVDARI 
       560        570        580        590        600
PDLAPPASDS TVTKLDFDLE PEPPPAPPRA SSLGETDESS ELRVLDIPGQ 
       610        620        630        640        650
GSETVFNIEN DRNNLRPKSK SEYELSIPDS GRSCWGVGEL DDKRAQQRFQ 
       660        670        680        690        700
FSLQDFRCCA VLGRGHFGKV LLAEYKHTNE MFAIKALKKG DIVARDEVDS 
       710        720        730        740        750
LMCEKRIFET VNSVRHPFLV NLFACFQTKE HVCFVMEYAA GGDLMMHIHT 
       760        770        780        790        800
DVFSEPRAVF YAACVVLGLQ YLHEHKIVYR DLKLDNLLLD TEGFVKIADF 
       810        820        830        840        850
GLCKEGMGYG DRTSTFCGTP EFLAPEVLTE TSYTRAVDWW GLGVLIYEML 
       860        870        880        890        900
VGESPFPGDD EEEVFDSIVN DEVRYPRFLS TEAISIMRRL LRRNPERRLG 
       910        920        930        940        950
AGEKDAEDVK KHPFFRLTDW SALMDKKVKP PFVPTIRGRE DVSNFDDEFT 
       960        970        980 
SEAPILTPPR EPRILLEEEQ EMFHDFDYVA DWC

Length:983

Mass (Da):111,607

Last modified:July 27, 2011 - v3

Checksum:ⁱ3961FD79D2718609

GO

Isoform 2 (identifier: Q8BWW9-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
35-45: Missing.

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        10         20         30         40         50
MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDDIKDR IKREIRKELK 
        60         70         80         90        100
IKEGAENLRK VTTDKKNLAY VDNILKKSNK KLEELHHKLQ ELNAHIVVSD 
       110        120        130        140        150
PEDSTDCPRT PDTPNSDSRS STSNNRLMAL QKQLDIELKV KQGAENMIQM 
       160        170        180        190        200
YSNGSSKDRK LHGTAQQLLQ DSKTKIEVIR MQILQAVQTN ELAFDNAKPV 
       210        220        230        240        250
ISPLELRMEE LRHHFKIEFA VAEGAKNVMK LLGSGKVTDR KALSEAQARF 
       260        270        280        290        300
NESSQKLDLL KYSLEQRLNE LPRNHPKSSV VIEELSLVAS PTLSPRQSML 
       310        320        330        340        350
STQNQYSTLS KPAALTGTLE VRLMGCQDIL ENVPGRSKAT SVALPGWSPS 
       360        370        380        390        400
DNRSSFMSRT SKSKSGSSRN LLKTDDLSND VCAVLKLDNT VVGQTSWKPI 
       410        420        430        440        450
SNQSWDQKFT LELDRSRELE ISVYWRDWRS LCAVKFLRLE DFLDNQRHGM 
       460        470        480        490        500
CLYLEPQGTL FAEVTFFNPV IERRPKLQRQ KKIFSKQQGK TFLRAPQMNI 
       510        520        530        540        550
NIATWGRLVR RAIPTVNHSG TFSPQTPVPA TVPVVDARIP DLAPPASDST 
       560        570        580        590        600
VTKLDFDLEP EPPPAPPRAS SLGETDESSE LRVLDIPGQG SETVFNIEND 
       610        620        630        640        650
RNNLRPKSKS EYELSIPDSG RSCWGVGELD DKRAQQRFQF SLQDFRCCAV 
       660        670        680        690        700
LGRGHFGKVL LAEYKHTNEM FAIKALKKGD IVARDEVDSL MCEKRIFETV 
       710        720        730        740        750
NSVRHPFLVN LFACFQTKEH VCFVMEYAAG GDLMMHIHTD VFSEPRAVFY 
       760        770        780        790        800
AACVVLGLQY LHEHKIVYRD LKLDNLLLDT EGFVKIADFG LCKEGMGYGD 
       810        820        830        840        850
RTSTFCGTPE FLAPEVLTET SYTRAVDWWG LGVLIYEMLV GESPFPGDDE 
       860        870        880        890        900
EEVFDSIVND EVRYPRFLST EAISIMRRLL RRNPERRLGA GEKDAEDVKK 
       910        920        930        940        950
HPFFRLTDWS ALMDKKVKPP FVPTIRGRED VSNFDDEFTS EAPILTPPRE 
       960        970 
PRILLEEEQE MFHDFDYVAD WC

Note: No experimental confirmation available.

Show »

Length:972

Mass (Da):110,313

Checksum:ⁱD2806BCD710C1B29

GO

Isoform 3 (identifier: Q8BWW9-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
781-787: DLKLDNL → NTIFSSI
788-983: Missing.

« Hide

        10         20         30         40         50
MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDFSDTM VQQKLDDIKD 
        60         70         80         90        100
RIKREIRKEL KIKEGAENLR KVTTDKKNLA YVDNILKKSN KKLEELHHKL 
       110        120        130        140        150
QELNAHIVVS DPEDSTDCPR TPDTPNSDSR SSTSNNRLMA LQKQLDIELK 
       160        170        180        190        200
VKQGAENMIQ MYSNGSSKDR KLHGTAQQLL QDSKTKIEVI RMQILQAVQT 
       210        220        230        240        250
NELAFDNAKP VISPLELRME ELRHHFKIEF AVAEGAKNVM KLLGSGKVTD 
       260        270        280        290        300
RKALSEAQAR FNESSQKLDL LKYSLEQRLN ELPRNHPKSS VVIEELSLVA 
       310        320        330        340        350
SPTLSPRQSM LSTQNQYSTL SKPAALTGTL EVRLMGCQDI LENVPGRSKA 
       360        370        380        390        400
TSVALPGWSP SDNRSSFMSR TSKSKSGSSR NLLKTDDLSN DVCAVLKLDN 
       410        420        430        440        450
TVVGQTSWKP ISNQSWDQKF TLELDRSREL EISVYWRDWR SLCAVKFLRL 
       460        470        480        490        500
EDFLDNQRHG MCLYLEPQGT LFAEVTFFNP VIERRPKLQR QKKIFSKQQG 
       510        520        530        540        550
KTFLRAPQMN INIATWGRLV RRAIPTVNHS GTFSPQTPVP ATVPVVDARI 
       560        570        580        590        600
PDLAPPASDS TVTKLDFDLE PEPPPAPPRA SSLGETDESS ELRVLDIPGQ 
       610        620        630        640        650
GSETVFNIEN DRNNLRPKSK SEYELSIPDS GRSCWGVGEL DDKRAQQRFQ 
       660        670        680        690        700
FSLQDFRCCA VLGRGHFGKV LLAEYKHTNE MFAIKALKKG DIVARDEVDS 
       710        720        730        740        750
LMCEKRIFET VNSVRHPFLV NLFACFQTKE HVCFVMEYAA GGDLMMHIHT 
       760        770        780 
DVFSEPRAVF YAACVVLGLQ YLHEHKIVYR NTIFSSI

Show »

Length:787

Mass (Da):88,961

Checksum:ⁱ6B762C942B96D5EF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	32	Q → H in BAC32655 (PubMed:16141072).Curated	1
Sequence conflictⁱ	84	N → H in BAC33888 (PubMed:16141072).Curated	1
Sequence conflictⁱ	395	V → D in BAC38910 (PubMed:16141072).Curated	1
Sequence conflictⁱ	569	L → F in BAC32655 (PubMed:16141072).Curated	1
Sequence conflictⁱ	704	E → K in BAC33888 (PubMed:16141072).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_012042	35 – 45	Missing in isoform 2. 1 PublicationAdd BLAST	11
Alternative sequenceⁱVSP_042185	781 – 787	DLKLDNL → NTIFSSI in isoform 3. 1 Publication	7
Alternative sequenceⁱVSP_042186	788 – 983	Missing in isoform 3. 1 PublicationAdd BLAST	196

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK046248 mRNA. Translation: BAC32655.1. AK133298 mRNA. Translation: BAE21599.1. AK171092 mRNA. Translation: BAE42244.1. AK049713 mRNA. Translation: BAC33888.1. AK083425 mRNA. Translation: BAC38910.1. BC052073 mRNA. Translation: AAH52073.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS51084.1. [Q8BWW9-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_848769.2. NM_178654.4. [Q8BWW9-1]
UniGeneⁱ	Mm.244236.

Genome annotation databases

Ensemblⁱ	ENSMUST00000043812; ENSMUSP00000039566; ENSMUSG00000004591. [Q8BWW9-1]
GeneIDⁱ	109333.
KEGGⁱ	mmu:109333.
UCSC genome browser More...UCSCⁱ	uc008rpe.2. mouse. [Q8BWW9-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8BWW9	Q8BW55 Q8BJM4	Mus musculus (Mouse)	983	UniRef100_Q8BWW9	Cluster: Serine/threonine-protein kinase N2	3

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8BWW9	Q16513 Q16513-4 A0A061ILT0 A0A2I2UHS1 A0A061INP9 F7C0E0 G8HFV6 UPI00027F2203 L5K3M6 F6S2E6 +167	Homo sapiens (Human) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Felis catus (Cat) (Felis silvestris catus) Macaca mulatta (Rhesus macaque) Mandrillus leucophaeus (Drill) (Papio leucophaeus) Pteropus alecto (Black flying fox) Equus caballus (Horse) Myotis brandtii (Brandt's bat) Rattus norvegicus (Rat) Aquila chrysaetos canadensis And more	984	UniRef90_Q16513	Cluster: Serine/threonine-protein kinase N2	178
Q8BWW9-3	UPI0009515D96 UPI000BB92D2C UPI000529E434 A0A1S3FUC7 UPI000703D7E1 UPI00080A2D0F UPI0006D71055 A0A093FJD4 UPI000A307D2A A0A091KLF8	Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus) Delphinapterus leucas (Beluga whale) Colius striatus (Speckled mousebird) Dipodomys ordii (Ord's kangaroo rat) Myotis brandtii (Brandt's bat) Cebus capucinus imitator Myotis lucifugus (Little brown bat) Gavia stellata (Red-throated diver) (Red-throated loon) Mus pahari (Gairdner's shrew-mouse) (Coelomys pahari) Chlamydotis macqueenii (Macqueen's bustard)	787	UniRef90_Q8BWW9-3	Cluster: Isoform 3 of Serine/threonine-protein kinase N2	11

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8BWW9	Q16513 Q16513-4 A0A061ILT0 A0A2I2UHS1 A0A061INP9 F7C0E0 G8HFV6 UPI00027F2203 L5K3M6 F6S2E6 +339	Homo sapiens (Human) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Felis catus (Cat) (Felis silvestris catus) Macaca mulatta (Rhesus macaque) Mandrillus leucophaeus (Drill) (Papio leucophaeus) Pteropus alecto (Black flying fox) Equus caballus (Horse) Myotis brandtii (Brandt's bat) Aquila chrysaetos canadensis Rattus norvegicus (Rat) And more	984	UniRef50_Q16513	Cluster: Serine/threonine-protein kinase N2	350
Q8BWW9-3	UPI000BB92D2C UPI0009515D96 UPI000529E434 A0A1S3FUC7 UPI000703D7E1 UPI00080A2D0F UPI0006D71055 A0A093FJD4 UPI000A307D2A A0A091KLF8 +247	Delphinapterus leucas (Beluga whale) Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus) Colius striatus (Speckled mousebird) Dipodomys ordii (Ord's kangaroo rat) Myotis brandtii (Brandt's bat) Cebus capucinus imitator Myotis lucifugus (Little brown bat) Gavia stellata (Red-throated diver) (Red-throated loon) Mus pahari (Gairdner's shrew-mouse) (Coelomys pahari) Chlamydotis macqueenii (Macqueen's bustard) And more	787	UniRef50_Q8BWW9-3	Cluster: Isoform 3 of Serine/threonine-protein kinase N2	258

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK046248 mRNA. Translation: BAC32655.1. AK133298 mRNA. Translation: BAE21599.1. AK171092 mRNA. Translation: BAE42244.1. AK049713 mRNA. Translation: BAC33888.1. AK083425 mRNA. Translation: BAC38910.1. BC052073 mRNA. Translation: AAH52073.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS51084.1. [Q8BWW9-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_848769.2. NM_178654.4. [Q8BWW9-1]
UniGeneⁱ	Mm.244236.

3D structure databases

ProteinModelPortalⁱ	Q8BWW9.
SMRⁱ	Q8BWW9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	224651. 2 interactors.
STRINGⁱ	10090.ENSMUSP00000039566.

PTM databases

iPTMnetⁱ	Q8BWW9.
PhosphoSitePlusⁱ	Q8BWW9.

Proteomic databases

EPDⁱ	Q8BWW9.
PaxDbⁱ	Q8BWW9.
PeptideAtlas More...PeptideAtlasⁱ	Q8BWW9.
PRIDEⁱ	Q8BWW9.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000043812; ENSMUSP00000039566; ENSMUSG00000004591. [Q8BWW9-1]
GeneIDⁱ	109333.
KEGGⁱ	mmu:109333.
UCSC genome browser More...UCSCⁱ	uc008rpe.2. mouse. [Q8BWW9-1]

Organism-specific databases

CTDⁱ	5586.
MGIⁱ	MGI:109211. Pkn2.

Phylogenomic databases

eggNOGⁱ	KOG0694. Eukaryota. ENOG410XNPH. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00820000126964.
HOGENOMⁱ	HOG000233032.
HOVERGENⁱ	HBG108317.
InParanoidⁱ	Q8BWW9.
KEGG Orthology (KO) More...KOⁱ	K06071.
OMAⁱ	RSQQMFQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G00YT.
TreeFamⁱ	TF102005.

Enzyme and pathway databases

Reactomeⁱ	R-MMU-5625740. RHO GTPases activate PKNs.

Reactomeⁱ

R-MMU-5625740. RHO GTPases activate PKNs.

Miscellaneous databases

ChiTaRSⁱ	Pkn2. mouse.
Protein Ontology More...PROⁱ	PR:Q8BWW9.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000004591.
CleanExⁱ	MM_PKN2.
ExpressionAtlasⁱ	Q8BWW9. baseline and differential.
Genevisibleⁱ	Q8BWW9. MM.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd08687. C2_PKN-like. 1 hit. cd11622. HR1_PKN_1. 1 hit.
InterProⁱ	View protein in InterPro IPR000961. AGC-kinase_C. IPR000008. C2_dom. IPR037784. C2_PKN. IPR011072. HR1_rho-bd. IPR036274. HR1_rpt_sf. IPR011009. Kinase-like_dom_sf. IPR017892. Pkinase_C. IPR037313. PKN_HR1_1. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit.
SMARTⁱ	View protein in SMART SM00239. C2. 1 hit. SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF46585. SSF46585. 3 hits. SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PKN2_MOUSE
Accessionⁱ	Q8BWW9Primary (citable) accession number: Q8BWW9 Secondary accession number(s): Q3TBR3 , Q80WS2, Q8BJL7, Q8BL62
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
	Last sequence update:	July 27, 2011
	Last modified:	March 28, 2018
	This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

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UniProtKB - Q8BWW9 (PKN2_MOUSE)

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Serine/threonine-protein kinase N2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

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<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

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<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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