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Q8BWU5

- OSGEP_MOUSE

UniProt

Q8BWU5 - OSGEP_MOUSE

Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase

Gene

Osgep

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (24 May 2004)
      Previous versions | rss
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    Functioni

    Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

    Catalytic activityi

    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.

    Cofactori

    Binds 1 divalent metal cation per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091Divalent metal cationUniRule annotation
    Metal bindingi113 – 1131Divalent metal cationUniRule annotation
    Metal bindingi130 – 1301Divalent metal cationUniRule annotation
    Binding sitei162 – 1621SubstrateUniRule annotation
    Binding sitei177 – 1771Substrate; via amide nitrogenUniRule annotation
    Binding sitei181 – 1811SubstrateUniRule annotation
    Binding sitei266 – 2661SubstrateUniRule annotation
    Metal bindingi294 – 2941Divalent metal cationUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    O-sialoglycoprotein endopeptidaseUniRule annotation
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein OsgepUniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein OsgepUniRule annotation
    Gene namesi
    Name:Osgep
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1913496. Osgep.

    Subcellular locationi

    Cytoplasm UniRule annotation. Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Probable tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096985Add
    BLAST

    Proteomic databases

    MaxQBiQ8BWU5.
    PaxDbiQ8BWU5.
    PRIDEiQ8BWU5.

    PTM databases

    PhosphoSiteiQ8BWU5.

    Expressioni

    Tissue specificityi

    Widely expressed at low level. Expressed at intermediate level in lung. Weakly expressed in testis, skeletal muscle, kidney, liver, spleen, brain and heart.1 Publication

    Developmental stagei

    Weakly expressed in 7, 11 and 17 day embryos. Expressed at a higher level in 15 day embryos.1 Publication

    Gene expression databases

    BgeeiQ8BWU5.
    CleanExiMM_OSGEP.
    GenevestigatoriQ8BWU5.

    Interactioni

    Subunit structurei

    Component of the EKC/KEOPS complex composed of at least TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BWU5.
    SMRiQ8BWU5. Positions 5-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1345Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the KAE1 / TsaD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    GeneTreeiENSGT00550000074933.
    HOGENOMiHOG000109569.
    HOVERGENiHBG051713.
    InParanoidiQ8BWU5.
    KOiK15900.
    OrthoDBiEOG7MD4Q9.
    PhylomeDBiQ8BWU5.
    TreeFamiTF313621.

    Family and domain databases

    HAMAPiMF_01446. Kae1_arch.
    InterProiIPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PRINTSiPR00789. OSIALOPTASE.
    TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BWU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAVLGFEGS ANKIGVGVVR DGTVLANPRR TYVTAPGTGF LPGDTARHHR    50
    AVILDLLQEA LAEAGLTSKD IDCIAFTKGP GMGAPLASVA VVARTVAQLW 100
    NKPLLGVNHC IGHIEMGRLI TGAVNPTVLY VSGGNTQVIS YSEHRYRIFG 150
    ETIDIAVGNC LDRFARVLKI SNDPSPGYNI EQMAKRGKKL VELPYTVKGM 200
    DVSFSGILSF IEDAAQRMLA TGECTPEDLC FSLQETVFAM LVEITERAMA 250
    HCGSKEALIV GGVGCNLRLQ EMMGTMCQER GAQLFATDER FCVDNGAMIA 300
    QAGWEMFQAG HRTPLKDSAI TQRYRTDEVE VTWRD 335
    Length:335
    Mass (Da):36,301
    Last modified:May 24, 2004 - v2
    Checksum:i906F58C5AF53A154
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621A → T in BAC34005. (PubMed:16141072)Curated
    Sequence conflicti84 – 841A → S in BAC34005. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK049955 mRNA. Translation: BAC34005.1.
    BC002296 mRNA. Translation: AAH02296.1.
    CCDSiCCDS27026.1.
    RefSeqiNP_598437.2. NM_133676.2.
    UniGeneiMm.274791.

    Genome annotation databases

    EnsembliENSMUST00000159292; ENSMUSP00000124039; ENSMUSG00000006289.
    ENSMUST00000160393; ENSMUSP00000125155; ENSMUSG00000006289.
    GeneIDi66246.
    KEGGimmu:66246.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK049955 mRNA. Translation: BAC34005.1 .
    BC002296 mRNA. Translation: AAH02296.1 .
    CCDSi CCDS27026.1.
    RefSeqi NP_598437.2. NM_133676.2.
    UniGenei Mm.274791.

    3D structure databases

    ProteinModelPortali Q8BWU5.
    SMRi Q8BWU5. Positions 5-333.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BWU5.

    Proteomic databases

    MaxQBi Q8BWU5.
    PaxDbi Q8BWU5.
    PRIDEi Q8BWU5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159292 ; ENSMUSP00000124039 ; ENSMUSG00000006289 .
    ENSMUST00000160393 ; ENSMUSP00000125155 ; ENSMUSG00000006289 .
    GeneIDi 66246.
    KEGGi mmu:66246.

    Organism-specific databases

    CTDi 55644.
    MGIi MGI:1913496. Osgep.

    Phylogenomic databases

    eggNOGi COG0533.
    GeneTreei ENSGT00550000074933.
    HOGENOMi HOG000109569.
    HOVERGENi HBG051713.
    InParanoidi Q8BWU5.
    KOi K15900.
    OrthoDBi EOG7MD4Q9.
    PhylomeDBi Q8BWU5.
    TreeFami TF313621.

    Miscellaneous databases

    NextBioi 321079.
    PROi Q8BWU5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BWU5.
    CleanExi MM_OSGEP.
    Genevestigatori Q8BWU5.

    Family and domain databases

    HAMAPi MF_01446. Kae1_arch.
    InterProi IPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PRINTSi PR00789. OSIALOPTASE.
    TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Hippocampus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    3. "Identification of the functional elements in the bidirectional promoter of the mouse O-sialoglycoprotein endopeptidase and APEX nuclease genes."
      Ikeda S., Ayabe H., Mori K., Seki Y., Seki S.
      Biochem. Biophys. Res. Commun. 296:785-791(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiOSGEP_MOUSE
    AccessioniPrimary (citable) accession number: Q8BWU5
    Secondary accession number(s): Q99LN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: May 24, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3