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Protein

3-ketoacyl-CoA thiolase, mitochondrial

Gene

Acaa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Abolishes BNIP3-mediated apoptosis and mitochondrial damage.By similarity

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Acyl-thioester intermediateBy similarity1
Active sitei352Proton acceptorPROSITE-ProRule annotation1
Active sitei382Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-77289. Mitochondrial Fatty Acid Beta-Oxidation.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase, mitochondrial (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Mitochondrial 3-oxoacyl-CoA thiolase
Gene namesi
Name:Acaa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1098623. Acaa2.

Subcellular locationi

  • Mitochondrion By similarity

  • Note: Colocalizes with BNIP3 in the mitochondria.By similarity

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233001 – 3973-ketoacyl-CoA thiolase, mitochondrialAdd BLAST397
Transit peptidei1 – 16Mitochondrion; not cleavedBy similarityAdd BLAST16

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25N6-acetyllysine; alternateCombined sources1
Modified residuei25N6-succinyllysine; alternateCombined sources1
Modified residuei28PhosphoserineCombined sources1
Modified residuei45N6-succinyllysineCombined sources1
Modified residuei119PhosphothreonineBy similarity1
Modified residuei121PhosphoserineBy similarity1
Modified residuei127PhosphotyrosineBy similarity1
Modified residuei136PhosphothreonineCombined sources1
Modified residuei137N6-acetyllysine; alternateCombined sources1
Modified residuei137N6-succinyllysine; alternateCombined sources1
Modified residuei143N6-acetyllysine; alternateCombined sources1
Modified residuei143N6-succinyllysine; alternateCombined sources1
Modified residuei158N6-acetyllysine; alternateCombined sources1
Modified residuei158N6-succinyllysine; alternateCombined sources1
Modified residuei171N6-acetyllysine; alternateCombined sources1
Modified residuei171N6-succinyllysine; alternateCombined sources1
Modified residuei191N6-acetyllysine; alternateCombined sources1
Modified residuei191N6-succinyllysine; alternateCombined sources1
Modified residuei209N6-acetyllysine; alternateCombined sources1
Modified residuei209N6-succinyllysine; alternateCombined sources1
Modified residuei211N6-succinyllysineCombined sources1
Modified residuei212N6-succinyllysineCombined sources1
Modified residuei214N6-succinyllysineCombined sources1
Modified residuei234N6-acetyllysine; alternateCombined sources1
Modified residuei234N6-succinyllysine; alternateCombined sources1
Modified residuei240N6-succinyllysineCombined sources1
Modified residuei241N6-acetyllysineCombined sources1
Modified residuei269N6-acetyllysineCombined sources1
Modified residuei270N6-acetyllysineCombined sources1
Modified residuei305N6-acetyllysine; alternateCombined sources1
Modified residuei305N6-succinyllysine; alternateCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei312N6-acetyllysine; alternateCombined sources1
Modified residuei312N6-succinyllysine; alternateCombined sources1
Modified residuei340N6-acetyllysineCombined sources1
Modified residuei344PhosphoserineCombined sources1
Modified residuei375N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BWT1.
MaxQBiQ8BWT1.
PaxDbiQ8BWT1.
PeptideAtlasiQ8BWT1.
PRIDEiQ8BWT1.

2D gel databases

REPRODUCTION-2DPAGEQ8BWT1.

PTM databases

iPTMnetiQ8BWT1.
PhosphoSitePlusiQ8BWT1.
SwissPalmiQ8BWT1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036880.
GenevisibleiQ8BWT1. MM.

Interactioni

Subunit structurei

Homotetramer. Interacts with BNIP3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi206649. 5 interactors.
IntActiQ8BWT1. 9 interactors.
MINTiMINT-1839761.
STRINGi10090.ENSMUSP00000037348.

Structurei

3D structure databases

ProteinModelPortaliQ8BWT1.
SMRiQ8BWT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ8BWT1.
KOiK07508.
OMAiMGITAEI.
OrthoDBiEOG091G09C6.
TreeFamiTF105696.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BWT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLRGVFIV AAKRTPFGAY GGLLKDFSAT DLTEFAARAA LSAGKVPPET
60 70 80 90 100
IDSVIVGNVM QSSSDAAYLA RHVGLRVGVP TETGALTLNR LCGSGFQSIV
110 120 130 140 150
SGCQEICSKD AEVVLCGGTE SMSQSPYCVR NVRFGTKFGL DLKLEDTLWA
160 170 180 190 200
GLTDQHVKLP MGMTAENLAA KYNISREDCD RYALQSQQRW KAANEAGYFN
210 220 230 240 250
EEMAPIEVKT KKGKQTMQVD EHARPQTTLE QLQKLPSVFK KDGTVTAGNA
260 270 280 290 300
SGVSDGAGAV IIASEDAVKK HNFTPLARVV GYFVSGCDPT IMGIGPVPAI
310 320 330 340 350
NGALKKAGLS LKDMDLIDVN EAFAPQFLSV QKALDLDPSK TNVSGGAIAL
360 370 380 390
GHPLGGSGSR ITAHLVHELR RRGGKYAVGS ACIGGGQGIA LIIQNTA
Length:397
Mass (Da):41,830
Last modified:July 27, 2011 - v3
Checksum:i856840546F5CB8DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti397A → V in AAH28901 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050101 mRNA. Translation: BAC34067.1.
AK167567 mRNA. Translation: BAE39630.1.
AK167715 mRNA. Translation: BAE39757.1.
AK169359 mRNA. Translation: BAE41108.1.
CH466528 Genomic DNA. Translation: EDL09521.1.
BC028901 mRNA. Translation: AAH28901.1.
CCDSiCCDS29342.1.
RefSeqiNP_803421.1. NM_177470.3.
UniGeneiMm.245724.

Genome annotation databases

EnsembliENSMUST00000041053; ENSMUSP00000037348; ENSMUSG00000036880.
GeneIDi52538.
KEGGimmu:52538.
UCSCiuc008fpt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050101 mRNA. Translation: BAC34067.1.
AK167567 mRNA. Translation: BAE39630.1.
AK167715 mRNA. Translation: BAE39757.1.
AK169359 mRNA. Translation: BAE41108.1.
CH466528 Genomic DNA. Translation: EDL09521.1.
BC028901 mRNA. Translation: AAH28901.1.
CCDSiCCDS29342.1.
RefSeqiNP_803421.1. NM_177470.3.
UniGeneiMm.245724.

3D structure databases

ProteinModelPortaliQ8BWT1.
SMRiQ8BWT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206649. 5 interactors.
IntActiQ8BWT1. 9 interactors.
MINTiMINT-1839761.
STRINGi10090.ENSMUSP00000037348.

PTM databases

iPTMnetiQ8BWT1.
PhosphoSitePlusiQ8BWT1.
SwissPalmiQ8BWT1.

2D gel databases

REPRODUCTION-2DPAGEQ8BWT1.

Proteomic databases

EPDiQ8BWT1.
MaxQBiQ8BWT1.
PaxDbiQ8BWT1.
PeptideAtlasiQ8BWT1.
PRIDEiQ8BWT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041053; ENSMUSP00000037348; ENSMUSG00000036880.
GeneIDi52538.
KEGGimmu:52538.
UCSCiuc008fpt.1. mouse.

Organism-specific databases

CTDi10449.
MGIiMGI:1098623. Acaa2.

Phylogenomic databases

eggNOGiKOG1391. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00390000009412.
HOGENOMiHOG000012238.
HOVERGENiHBG003112.
InParanoidiQ8BWT1.
KOiK07508.
OMAiMGITAEI.
OrthoDBiEOG091G09C6.
TreeFamiTF105696.

Enzyme and pathway databases

UniPathwayiUPA00199.
ReactomeiR-MMU-77289. Mitochondrial Fatty Acid Beta-Oxidation.

Miscellaneous databases

PROiQ8BWT1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036880.
GenevisibleiQ8BWT1. MM.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_MOUSE
AccessioniPrimary (citable) accession number: Q8BWT1
Secondary accession number(s): Q3TIT9, Q8JZR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.