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Protein

Rhophilin-2

Gene

Rhpn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5666185. RHO GTPases Activate Rhotekin and Rhophilins.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhophilin-2
Alternative name(s):
GTP-Rho-binding protein 2
Gene namesi
Name:Rhpn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1289234. Rhpn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Rhophilin-2PRO_0000218899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei655 – 6551PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BWR8.
PaxDbiQ8BWR8.
PRIDEiQ8BWR8.

PTM databases

iPTMnetiQ8BWR8.
PhosphoSiteiQ8BWR8.

Expressioni

Gene expression databases

BgeeiQ8BWR8.
CleanExiMM_RHPN2.
GenevisibleiQ8BWR8. MM.

Interactioni

Subunit structurei

Interacts with GTP-bound RhoA and RhoB. Interacts with both GTP- and GDP-bound RhoA. Interacts with KRT18 (By similarity).By similarity

Protein-protein interaction databases

BioGridi206579. 3 interactions.
IntActiQ8BWR8. 1 interaction.
MINTiMINT-4132405.
STRINGi10090.ENSMUSP00000032705.

Structurei

Secondary structure

1
686
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi530 – 5356Combined sources
Helixi547 – 5515Combined sources
Beta strandi557 – 5615Combined sources
Helixi571 – 58010Combined sources
Turni581 – 5833Combined sources
Beta strandi584 – 5863Combined sources
Beta strandi588 – 5914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VAENMR-A506-603[»]
ProteinModelPortaliQ8BWR8.
SMRiQ8BWR8. Positions 110-357, 509-608.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BWR8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati38 – 11073REMAdd
BLAST
Domaini111 – 502392BRO1PROSITE-ProRule annotationAdd
BLAST
Domaini515 – 59379PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni46 – 6621Interaction with RhoBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi623 – 6264Poly-Asp

Sequence similaritiesi

Belongs to the RHPN family.Curated
Contains 1 BRO1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 REM (Hr1) repeat.Curated

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOGENOMiHOG000046725.
HOVERGENiHBG067197.
InParanoidiQ8BWR8.
OMAiHYSEDSY.
OrthoDBiEOG7XDBF7.
PhylomeDBiQ8BWR8.
TreeFamiTF323502.

Family and domain databases

Gene3Di1.25.40.280. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR004328. BRO1_dom.
IPR011072. HR1_rho-bd.
IPR001478. PDZ.
[Graphical view]
PfamiPF03097. BRO1. 1 hit.
PF02185. HR1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
SM00742. Hr1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BWR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTLLPAAP QPLEKEGDDY FRKGCNPLAQ TGRSKLQNQR AALNQQILKA
60 70 80 90 100
VRMRTGAENL LKVATNQKVR EQVRLELSFV NSDLQMLKEE LEGLNISVGV
110 120 130 140 150
YQGTEEAFTI PLIPLGLKET KEVDFSIVFK DFILEHYSED SYLYEDDIAD
160 170 180 190 200
LMDLRQACRT PSRDEAGVEL LMSYFIQLGF VESRFFPPTR HMGLLFTWYD
210 220 230 240 250
SFTGVPVSQQ TLLLEKASVL FNIGALYTQI GTRCNRQTQA GLESAVDAFQ
260 270 280 290 300
RAAGVLNYLK ETFTHTPSYD MSPAMLSVLV KMMLAQAQES VFEKVCLPGI
310 320 330 340 350
QNEFFVLVKV AQEAAKVAEA YRQLHAAMSQ EPVKENIPYS WASVAYVKAY
360 370 380 390 400
HYGALAHYFA ATLLIDHQLK PGADEDHQEK CLSQLYDRMP EGMTPLATLK
410 420 430 440 450
NAGQRVLLGK GHLHRAIGFH EESLREANLC KKLRDIQVLR DVLSAAHQRT
460 470 480 490 500
QLKHTQHRED DDLLNLIDAP DVLPKTEREV KITFPDFSKV TVTDFFQKLG
510 520 530 540 550
PLSVFSASKR WSPPRGIHFT VEEGDLGFTL RGNTPVQVHF LDPHCSASLA
560 570 580 590 600
GAKEGDYIVS IQGVDCKWLT VSEVMKLLKS FGGEEVEMKV VSLLDSTSSM
610 620 630 640 650
HNKCATYSVG MQKTYSMICL SMDDDDKADK TKKISKKLSF LSWGTSKNRQ
660 670 680
KSASTLCLPE VGLARSQNKK KLPTPFSLLN SDSSLY
Length:686
Mass (Da):76,957
Last modified:February 1, 2005 - v2
Checksum:iBFEBEFB71D04F360
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551T → A in BAC34127 (PubMed:16141072).Curated
Sequence conflicti518 – 5181H → D in BAC34127 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004849 mRNA. Translation: BAB23615.1.
AK050214 mRNA. Translation: BAC34127.1.
CCDSiCCDS21148.1.
RefSeqiNP_082173.3. NM_027897.4.
UniGeneiMm.286600.

Genome annotation databases

EnsembliENSMUST00000032705; ENSMUSP00000032705; ENSMUSG00000030494.
ENSMUST00000085556; ENSMUSP00000082692; ENSMUSG00000030494.
GeneIDi52428.
KEGGimmu:52428.
UCSCiuc009gjs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004849 mRNA. Translation: BAB23615.1.
AK050214 mRNA. Translation: BAC34127.1.
CCDSiCCDS21148.1.
RefSeqiNP_082173.3. NM_027897.4.
UniGeneiMm.286600.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VAENMR-A506-603[»]
ProteinModelPortaliQ8BWR8.
SMRiQ8BWR8. Positions 110-357, 509-608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206579. 3 interactions.
IntActiQ8BWR8. 1 interaction.
MINTiMINT-4132405.
STRINGi10090.ENSMUSP00000032705.

PTM databases

iPTMnetiQ8BWR8.
PhosphoSiteiQ8BWR8.

Proteomic databases

MaxQBiQ8BWR8.
PaxDbiQ8BWR8.
PRIDEiQ8BWR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032705; ENSMUSP00000032705; ENSMUSG00000030494.
ENSMUST00000085556; ENSMUSP00000082692; ENSMUSG00000030494.
GeneIDi52428.
KEGGimmu:52428.
UCSCiuc009gjs.2. mouse.

Organism-specific databases

CTDi85415.
MGIiMGI:1289234. Rhpn2.

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOGENOMiHOG000046725.
HOVERGENiHBG067197.
InParanoidiQ8BWR8.
OMAiHYSEDSY.
OrthoDBiEOG7XDBF7.
PhylomeDBiQ8BWR8.
TreeFamiTF323502.

Enzyme and pathway databases

ReactomeiR-MMU-5666185. RHO GTPases Activate Rhotekin and Rhophilins.

Miscellaneous databases

ChiTaRSiRhpn2. mouse.
EvolutionaryTraceiQ8BWR8.
NextBioi308938.
PROiQ8BWR8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BWR8.
CleanExiMM_RHPN2.
GenevisibleiQ8BWR8. MM.

Family and domain databases

Gene3Di1.25.40.280. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR004328. BRO1_dom.
IPR011072. HR1_rho-bd.
IPR001478. PDZ.
[Graphical view]
PfamiPF03097. BRO1. 1 hit.
PF02185. HR1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
SM00742. Hr1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51180. BRO1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Liver, Lung, Pancreas and Testis.
  4. "Solution structure of the PDZ domain of mouse rhophilin-2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 506-603.

Entry informationi

Entry nameiRHPN2_MOUSE
AccessioniPrimary (citable) accession number: Q8BWR8
Secondary accession number(s): Q9DBN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: January 20, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.