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Protein

Ubiquitin carboxyl-terminal hydrolase 40

Gene

Usp40

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50NucleophilePROSITE-ProRule annotation1
Active sitei305Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC19.069.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 40 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 40
Ubiquitin thioesterase 40
Ubiquitin-specific-processing protease 40
Gene namesi
Name:Usp40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443184. Usp40.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806711 – 1235Ubiquitin carboxyl-terminal hydrolase 40Add BLAST1235

Proteomic databases

PaxDbiQ8BWR4.
PeptideAtlasiQ8BWR4.
PRIDEiQ8BWR4.

PTM databases

iPTMnetiQ8BWR4.
PhosphoSitePlusiQ8BWR4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005501.
CleanExiMM_USP40.
ExpressionAtlasiQ8BWR4. baseline and differential.
GenevisibleiQ8BWR4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BWR4. 1 interactor.
MINTiMINT-4139230.
STRINGi10090.ENSMUSP00000038533.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 482USPAdd BLAST442

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1863. Eukaryota.
COG5077. LUCA.
GeneTreeiENSGT00760000119158.
HOVERGENiHBG079302.
InParanoidiQ8BWR4.
KOiK11869.
OMAiVIIHKGG.
OrthoDBiEOG091G00MP.
PhylomeDBiQ8BWR4.
TreeFamiTF106281.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BWR4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGNLFEEDY SSVSSSQYGR GKKLKTKGLE PPAPREFTNL SGIRNQGGTC
60 70 80 90 100
YLSSLLQTLH FTPEFREALF SLGPEELGSL EDKDKPDAKV RIIPLQLQRL
110 120 130 140 150
FAQLLLLDQE AASTIDLTDS FGWTNDEEMR QHDVQELNRI LFSALETSLV
160 170 180 190 200
GTSGHDLIHR LYHGTIVNQI VCKECKNISE RQEDFLDLTV AVKNVSGLED
210 220 230 240 250
ELCNMYVEEE IFDYDNLYHC GTCDRLVKAA KSAKLRKLPP FLTISLLRFN
260 270 280 290 300
FDFVKCERYK DTSCYTFPLR INLKPFCEQS ELDDMEYMYD LFSVIIHKGG
310 320 330 340 350
CYGGHYHVYI KDVDHLGNWQ CQEEISDTNV NVKAPQSEEE ANDPLVVLKT
360 370 380 390 400
ILLQEEANQI PVDQLGQKLL KKTGISWNKK YRKQHGPLRK FLQLHPQIFL
410 420 430 440 450
LSTDESTVSL LRNHLTQAPS DLQSCEQILH TLASESPGLN DDTSCPHWFD
460 470 480 490 500
INDSKVHPIR EKDITQQFQG KESAYMLFYR KATLQRPPEA RANPRYRVPC
510 520 530 540 550
HLLKEMDAAN ILLQMRRAEC DSANSTFELH LHLGPHYRFF NGALHPAVSE
560 570 580 590 600
TESVWDLTFD KRKTLGDLRQ SIFQLLECWE GDMVLSVAKR VPAGLHVYHT
610 620 630 640 650
LDGDDLTLCE AEVADGEDIF VWNGVEVGGV QIQTGFDCEP LLLNILHLEL
660 670 680 690 700
SGEGSKCEQL VESPHVFPAN AEVGAVFTAL GTPAGAILMN SVESADGECW
710 720 730 740 750
TAVPKEDMKK TFREQGLRNG SLILVQDSDS DNNSLLPKQG RWTNSMNELN
760 770 780 790 800
WLQVKNFCQS ESEEKQVQIA VTMHTVVFDI RIKAIKELKL MKELAENSCL
810 820 830 840 850
RPIDRNGKLL CPVPDSSTLE EAEVKMGSSV GLCLGKAPTS SQLFLFFALG
860 870 880 890 900
TDIHPGAEMD IIVEETLSVR DCLKIMLEKS GQQGEIWHLR KMDWCYEAGE
910 920 930 940 950
PLCEEDATLK ELMIRSGDTL LLTEGKLPPP GHLKMPIWWY QPARLSGHCE
960 970 980 990 1000
SRDHLNCAFS QDSTWRAAPT QGAPGPEPAE VSLLYLGDME ISEEATLVEL
1010 1020 1030 1040 1050
KSQALALPSV SKLAVQSTAL LRVWTVESKR PSRLLRTNWR QLKEYRLGRR
1060 1070 1080 1090 1100
AELCLELLQK EEDLGPRDVL LRTQLRIPGE RAYSLATDLI WDTTRGWTAG
1110 1120 1130 1140 1150
SLRQRVADFY SLPVEKIEIA KYFPEKFEWL PISSWNQQVA KRKKKKNQDT
1160 1170 1180 1190 1200
LQGGPYYLKD GDTIGIKNLL FDDNDDFSTI RDDIGKENQK RLALEKKKSR
1210 1220 1230
EVHRAQSSDL FSNAGVPARF RGPEASLSIH VASFR
Length:1,235
Mass (Da):139,952
Last modified:January 9, 2007 - v2
Checksum:iCBAC7A6A0CACE03C
GO
Isoform 2 (identifier: Q8BWR4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     795-883: Missing.

Note: No experimental confirmation available.
Show »
Length:1,146
Mass (Da):130,389
Checksum:i91974BE0E09AF9B5
GO
Isoform 3 (identifier: Q8BWR4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.

Note: No experimental confirmation available.
Show »
Length:1,107
Mass (Da):125,625
Checksum:iF52FD5BAA6D8540A
GO
Isoform 4 (identifier: Q8BWR4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     490-501: ARANPRYRVPCH → GMENKKCSALLC
     502-1235: Missing.

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):57,394
Checksum:iDB8DAE538B27B9EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201E → A in BAE38691 (PubMed:16141072).Curated1
Sequence conflicti943A → E in AAH89376 (PubMed:15489334).Curated1
Sequence conflicti1134S → N in BAE38411 (PubMed:16141072).Curated1
Sequence conflicti1142R → K in AAH89376 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0221711 – 128Missing in isoform 3. 1 PublicationAdd BLAST128
Alternative sequenceiVSP_022172490 – 501ARANP…RVPCH → GMENKKCSALLC in isoform 4. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_022173502 – 1235Missing in isoform 4. 1 PublicationAdd BLAST734
Alternative sequenceiVSP_022174795 – 883Missing in isoform 2. 1 PublicationAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050239 mRNA. Translation: BAC34140.1.
AK132295 mRNA. Translation: BAE21086.1.
AK165849 mRNA. Translation: BAE38411.1.
AK166303 mRNA. Translation: BAE38691.1.
BC089376 mRNA. Translation: AAH89376.1.
BC117957 mRNA. Translation: AAI17958.1.
BC117958 mRNA. Translation: AAI17959.1.
CCDSiCCDS15137.1. [Q8BWR4-2]
CCDS78645.1. [Q8BWR4-1]
RefSeqiNP_001028463.1. NM_001033291.2. [Q8BWR4-2]
NP_001185502.1. NM_001198573.1. [Q8BWR4-1]
UniGeneiMm.80484.

Genome annotation databases

EnsembliENSMUST00000040783; ENSMUSP00000038533; ENSMUSG00000005501. [Q8BWR4-2]
ENSMUST00000187758; ENSMUSP00000140107; ENSMUSG00000005501. [Q8BWR4-1]
GeneIDi227334.
KEGGimmu:227334.
UCSCiuc007bxw.1. mouse. [Q8BWR4-2]
uc007bxx.1. mouse. [Q8BWR4-1]
uc007bxz.1. mouse. [Q8BWR4-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050239 mRNA. Translation: BAC34140.1.
AK132295 mRNA. Translation: BAE21086.1.
AK165849 mRNA. Translation: BAE38411.1.
AK166303 mRNA. Translation: BAE38691.1.
BC089376 mRNA. Translation: AAH89376.1.
BC117957 mRNA. Translation: AAI17958.1.
BC117958 mRNA. Translation: AAI17959.1.
CCDSiCCDS15137.1. [Q8BWR4-2]
CCDS78645.1. [Q8BWR4-1]
RefSeqiNP_001028463.1. NM_001033291.2. [Q8BWR4-2]
NP_001185502.1. NM_001198573.1. [Q8BWR4-1]
UniGeneiMm.80484.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BWR4. 1 interactor.
MINTiMINT-4139230.
STRINGi10090.ENSMUSP00000038533.

Protein family/group databases

MEROPSiC19.069.

PTM databases

iPTMnetiQ8BWR4.
PhosphoSitePlusiQ8BWR4.

Proteomic databases

PaxDbiQ8BWR4.
PeptideAtlasiQ8BWR4.
PRIDEiQ8BWR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040783; ENSMUSP00000038533; ENSMUSG00000005501. [Q8BWR4-2]
ENSMUST00000187758; ENSMUSP00000140107; ENSMUSG00000005501. [Q8BWR4-1]
GeneIDi227334.
KEGGimmu:227334.
UCSCiuc007bxw.1. mouse. [Q8BWR4-2]
uc007bxx.1. mouse. [Q8BWR4-1]
uc007bxz.1. mouse. [Q8BWR4-4]

Organism-specific databases

CTDi55230.
MGIiMGI:2443184. Usp40.

Phylogenomic databases

eggNOGiKOG1863. Eukaryota.
COG5077. LUCA.
GeneTreeiENSGT00760000119158.
HOVERGENiHBG079302.
InParanoidiQ8BWR4.
KOiK11869.
OMAiVIIHKGG.
OrthoDBiEOG091G00MP.
PhylomeDBiQ8BWR4.
TreeFamiTF106281.

Miscellaneous databases

ChiTaRSiUsp40. mouse.
PROiPR:Q8BWR4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005501.
CleanExiMM_USP40.
ExpressionAtlasiQ8BWR4. baseline and differential.
GenevisibleiQ8BWR4. MM.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBP40_MOUSE
AccessioniPrimary (citable) accession number: Q8BWR4
Secondary accession number(s): Q08EB7
, Q148V0, Q3TLV1, Q3TMN0, Q3V1R4, Q5EBN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: January 9, 2007
Last modified: June 7, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.