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Q8BWR4 (UBP40_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 40

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 40
Ubiquitin thioesterase 40
Ubiquitin-specific-processing protease 40
Gene names
Name:Usp40
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1235 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BWR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BWR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     795-883: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8BWR4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8BWR4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     490-501: ARANPRYRVPCH → GMENKKCSALLC
     502-1235: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12351235Ubiquitin carboxyl-terminal hydrolase 40
PRO_0000080671

Regions

Domain41 – 482442USP

Sites

Active site501Nucleophile By similarity
Active site3051Proton acceptor By similarity

Natural variations

Alternative sequence1 – 128128Missing in isoform 3.
VSP_022171
Alternative sequence490 – 50112ARANP…RVPCH → GMENKKCSALLC in isoform 4.
VSP_022172
Alternative sequence502 – 1235734Missing in isoform 4.
VSP_022173
Alternative sequence795 – 88389Missing in isoform 2.
VSP_022174

Experimental info

Sequence conflict2011E → A in BAE38691. Ref.1
Sequence conflict9431A → E in AAH89376. Ref.2
Sequence conflict11341S → N in BAE38411. Ref.1
Sequence conflict11421R → K in AAH89376. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: CBAC7A6A0CACE03C

FASTA1,235139,952
        10         20         30         40         50         60 
MFGNLFEEDY SSVSSSQYGR GKKLKTKGLE PPAPREFTNL SGIRNQGGTC YLSSLLQTLH 

        70         80         90        100        110        120 
FTPEFREALF SLGPEELGSL EDKDKPDAKV RIIPLQLQRL FAQLLLLDQE AASTIDLTDS 

       130        140        150        160        170        180 
FGWTNDEEMR QHDVQELNRI LFSALETSLV GTSGHDLIHR LYHGTIVNQI VCKECKNISE 

       190        200        210        220        230        240 
RQEDFLDLTV AVKNVSGLED ELCNMYVEEE IFDYDNLYHC GTCDRLVKAA KSAKLRKLPP 

       250        260        270        280        290        300 
FLTISLLRFN FDFVKCERYK DTSCYTFPLR INLKPFCEQS ELDDMEYMYD LFSVIIHKGG 

       310        320        330        340        350        360 
CYGGHYHVYI KDVDHLGNWQ CQEEISDTNV NVKAPQSEEE ANDPLVVLKT ILLQEEANQI 

       370        380        390        400        410        420 
PVDQLGQKLL KKTGISWNKK YRKQHGPLRK FLQLHPQIFL LSTDESTVSL LRNHLTQAPS 

       430        440        450        460        470        480 
DLQSCEQILH TLASESPGLN DDTSCPHWFD INDSKVHPIR EKDITQQFQG KESAYMLFYR 

       490        500        510        520        530        540 
KATLQRPPEA RANPRYRVPC HLLKEMDAAN ILLQMRRAEC DSANSTFELH LHLGPHYRFF 

       550        560        570        580        590        600 
NGALHPAVSE TESVWDLTFD KRKTLGDLRQ SIFQLLECWE GDMVLSVAKR VPAGLHVYHT 

       610        620        630        640        650        660 
LDGDDLTLCE AEVADGEDIF VWNGVEVGGV QIQTGFDCEP LLLNILHLEL SGEGSKCEQL 

       670        680        690        700        710        720 
VESPHVFPAN AEVGAVFTAL GTPAGAILMN SVESADGECW TAVPKEDMKK TFREQGLRNG 

       730        740        750        760        770        780 
SLILVQDSDS DNNSLLPKQG RWTNSMNELN WLQVKNFCQS ESEEKQVQIA VTMHTVVFDI 

       790        800        810        820        830        840 
RIKAIKELKL MKELAENSCL RPIDRNGKLL CPVPDSSTLE EAEVKMGSSV GLCLGKAPTS 

       850        860        870        880        890        900 
SQLFLFFALG TDIHPGAEMD IIVEETLSVR DCLKIMLEKS GQQGEIWHLR KMDWCYEAGE 

       910        920        930        940        950        960 
PLCEEDATLK ELMIRSGDTL LLTEGKLPPP GHLKMPIWWY QPARLSGHCE SRDHLNCAFS 

       970        980        990       1000       1010       1020 
QDSTWRAAPT QGAPGPEPAE VSLLYLGDME ISEEATLVEL KSQALALPSV SKLAVQSTAL 

      1030       1040       1050       1060       1070       1080 
LRVWTVESKR PSRLLRTNWR QLKEYRLGRR AELCLELLQK EEDLGPRDVL LRTQLRIPGE 

      1090       1100       1110       1120       1130       1140 
RAYSLATDLI WDTTRGWTAG SLRQRVADFY SLPVEKIEIA KYFPEKFEWL PISSWNQQVA 

      1150       1160       1170       1180       1190       1200 
KRKKKKNQDT LQGGPYYLKD GDTIGIKNLL FDDNDDFSTI RDDIGKENQK RLALEKKKSR 

      1210       1220       1230 
EVHRAQSSDL FSNAGVPARF RGPEASLSIH VASFR 

« Hide

Isoform 2 [UniParc].

Checksum: 91974BE0E09AF9B5
Show »

FASTA1,146130,389
Isoform 3 [UniParc].

Checksum: F52FD5BAA6D8540A
Show »

FASTA1,107125,625
Isoform 4 [UniParc].

Checksum: DB8DAE538B27B9EB
Show »

FASTA50157,394

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1140 AND 967-1235 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryo, Liver, Lung and Mammary gland.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6.
Tissue: Eye.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK050239 mRNA. Translation: BAC34140.1.
AK132295 mRNA. Translation: BAE21086.1.
AK165849 mRNA. Translation: BAE38411.1.
AK166303 mRNA. Translation: BAE38691.1.
BC089376 mRNA. Translation: AAH89376.1.
BC117957 mRNA. Translation: AAI17958.1.
BC117958 mRNA. Translation: AAI17959.1.
RefSeqNP_001028463.1. NM_001033291.2.
NP_001185502.1. NM_001198573.1.
XP_006529542.1. XM_006529479.1.
UniGeneMm.80484.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BWR4. 1 interaction.
MINTMINT-4139230.

Protein family/group databases

MEROPSC19.069.

PTM databases

PhosphoSiteQ8BWR4.

Proteomic databases

PaxDbQ8BWR4.
PRIDEQ8BWR4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040783; ENSMUSP00000038533; ENSMUSG00000005501. [Q8BWR4-2]
GeneID227334.
KEGGmmu:227334.
UCSCuc007bxw.1. mouse. [Q8BWR4-2]
uc007bxx.1. mouse. [Q8BWR4-1]
uc007bxz.1. mouse. [Q8BWR4-4]

Organism-specific databases

CTD55230.
MGIMGI:2443184. Usp40.

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00740000115066.
HOVERGENHBG079302.
InParanoidQ8BWR4.
KOK11869.
OMAVWDLTFD.
OrthoDBEOG7R2BHT.
PhylomeDBQ8BWR4.
TreeFamTF106281.

Gene expression databases

BgeeQ8BWR4.
CleanExMM_USP40.
GenevestigatorQ8BWR4.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio378568.
PROQ8BWR4.
SOURCESearch...

Entry information

Entry nameUBP40_MOUSE
AccessionPrimary (citable) accession number: Q8BWR4
Secondary accession number(s): Q08EB7 expand/collapse secondary AC list , Q148V0, Q3TLV1, Q3TMN0, Q3V1R4, Q5EBN6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: January 9, 2007
Last modified: April 16, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot