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Q8BWR4

- UBP40_MOUSE

UniProt

Q8BWR4 - UBP40_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 40

Gene

Usp40

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei50 – 501NucleophilePROSITE-ProRule annotation
    Active sitei305 – 3051Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.069.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 40 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 40
    Ubiquitin thioesterase 40
    Ubiquitin-specific-processing protease 40
    Gene namesi
    Name:Usp40
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2443184. Usp40.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12351235Ubiquitin carboxyl-terminal hydrolase 40PRO_0000080671Add
    BLAST

    Proteomic databases

    PaxDbiQ8BWR4.
    PRIDEiQ8BWR4.

    PTM databases

    PhosphoSiteiQ8BWR4.

    Expressioni

    Gene expression databases

    BgeeiQ8BWR4.
    CleanExiMM_USP40.
    GenevestigatoriQ8BWR4.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BWR4. 1 interaction.
    MINTiMINT-4139230.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 482442USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00740000115066.
    HOVERGENiHBG079302.
    InParanoidiQ8BWR4.
    KOiK11869.
    OMAiVWDLTFD.
    OrthoDBiEOG7R2BHT.
    PhylomeDBiQ8BWR4.
    TreeFamiTF106281.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BWR4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFGNLFEEDY SSVSSSQYGR GKKLKTKGLE PPAPREFTNL SGIRNQGGTC     50
    YLSSLLQTLH FTPEFREALF SLGPEELGSL EDKDKPDAKV RIIPLQLQRL 100
    FAQLLLLDQE AASTIDLTDS FGWTNDEEMR QHDVQELNRI LFSALETSLV 150
    GTSGHDLIHR LYHGTIVNQI VCKECKNISE RQEDFLDLTV AVKNVSGLED 200
    ELCNMYVEEE IFDYDNLYHC GTCDRLVKAA KSAKLRKLPP FLTISLLRFN 250
    FDFVKCERYK DTSCYTFPLR INLKPFCEQS ELDDMEYMYD LFSVIIHKGG 300
    CYGGHYHVYI KDVDHLGNWQ CQEEISDTNV NVKAPQSEEE ANDPLVVLKT 350
    ILLQEEANQI PVDQLGQKLL KKTGISWNKK YRKQHGPLRK FLQLHPQIFL 400
    LSTDESTVSL LRNHLTQAPS DLQSCEQILH TLASESPGLN DDTSCPHWFD 450
    INDSKVHPIR EKDITQQFQG KESAYMLFYR KATLQRPPEA RANPRYRVPC 500
    HLLKEMDAAN ILLQMRRAEC DSANSTFELH LHLGPHYRFF NGALHPAVSE 550
    TESVWDLTFD KRKTLGDLRQ SIFQLLECWE GDMVLSVAKR VPAGLHVYHT 600
    LDGDDLTLCE AEVADGEDIF VWNGVEVGGV QIQTGFDCEP LLLNILHLEL 650
    SGEGSKCEQL VESPHVFPAN AEVGAVFTAL GTPAGAILMN SVESADGECW 700
    TAVPKEDMKK TFREQGLRNG SLILVQDSDS DNNSLLPKQG RWTNSMNELN 750
    WLQVKNFCQS ESEEKQVQIA VTMHTVVFDI RIKAIKELKL MKELAENSCL 800
    RPIDRNGKLL CPVPDSSTLE EAEVKMGSSV GLCLGKAPTS SQLFLFFALG 850
    TDIHPGAEMD IIVEETLSVR DCLKIMLEKS GQQGEIWHLR KMDWCYEAGE 900
    PLCEEDATLK ELMIRSGDTL LLTEGKLPPP GHLKMPIWWY QPARLSGHCE 950
    SRDHLNCAFS QDSTWRAAPT QGAPGPEPAE VSLLYLGDME ISEEATLVEL 1000
    KSQALALPSV SKLAVQSTAL LRVWTVESKR PSRLLRTNWR QLKEYRLGRR 1050
    AELCLELLQK EEDLGPRDVL LRTQLRIPGE RAYSLATDLI WDTTRGWTAG 1100
    SLRQRVADFY SLPVEKIEIA KYFPEKFEWL PISSWNQQVA KRKKKKNQDT 1150
    LQGGPYYLKD GDTIGIKNLL FDDNDDFSTI RDDIGKENQK RLALEKKKSR 1200
    EVHRAQSSDL FSNAGVPARF RGPEASLSIH VASFR 1235
    Length:1,235
    Mass (Da):139,952
    Last modified:January 9, 2007 - v2
    Checksum:iCBAC7A6A0CACE03C
    GO
    Isoform 2 (identifier: Q8BWR4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         795-883: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,146
    Mass (Da):130,389
    Checksum:i91974BE0E09AF9B5
    GO
    Isoform 3 (identifier: Q8BWR4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-128: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,107
    Mass (Da):125,625
    Checksum:iF52FD5BAA6D8540A
    GO
    Isoform 4 (identifier: Q8BWR4-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         490-501: ARANPRYRVPCH → GMENKKCSALLC
         502-1235: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:501
    Mass (Da):57,394
    Checksum:iDB8DAE538B27B9EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti201 – 2011E → A in BAE38691. (PubMed:16141072)Curated
    Sequence conflicti943 – 9431A → E in AAH89376. (PubMed:15489334)Curated
    Sequence conflicti1134 – 11341S → N in BAE38411. (PubMed:16141072)Curated
    Sequence conflicti1142 – 11421R → K in AAH89376. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 128128Missing in isoform 3. 1 PublicationVSP_022171Add
    BLAST
    Alternative sequencei490 – 50112ARANP…RVPCH → GMENKKCSALLC in isoform 4. 1 PublicationVSP_022172Add
    BLAST
    Alternative sequencei502 – 1235734Missing in isoform 4. 1 PublicationVSP_022173Add
    BLAST
    Alternative sequencei795 – 88389Missing in isoform 2. 1 PublicationVSP_022174Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK050239 mRNA. Translation: BAC34140.1.
    AK132295 mRNA. Translation: BAE21086.1.
    AK165849 mRNA. Translation: BAE38411.1.
    AK166303 mRNA. Translation: BAE38691.1.
    BC089376 mRNA. Translation: AAH89376.1.
    BC117957 mRNA. Translation: AAI17958.1.
    BC117958 mRNA. Translation: AAI17959.1.
    CCDSiCCDS15137.1. [Q8BWR4-2]
    RefSeqiNP_001028463.1. NM_001033291.2. [Q8BWR4-2]
    NP_001185502.1. NM_001198573.1. [Q8BWR4-1]
    XP_006529542.1. XM_006529479.1. [Q8BWR4-3]
    UniGeneiMm.80484.

    Genome annotation databases

    EnsembliENSMUST00000040783; ENSMUSP00000038533; ENSMUSG00000005501. [Q8BWR4-2]
    GeneIDi227334.
    KEGGimmu:227334.
    UCSCiuc007bxw.1. mouse. [Q8BWR4-2]
    uc007bxx.1. mouse. [Q8BWR4-1]
    uc007bxz.1. mouse. [Q8BWR4-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK050239 mRNA. Translation: BAC34140.1 .
    AK132295 mRNA. Translation: BAE21086.1 .
    AK165849 mRNA. Translation: BAE38411.1 .
    AK166303 mRNA. Translation: BAE38691.1 .
    BC089376 mRNA. Translation: AAH89376.1 .
    BC117957 mRNA. Translation: AAI17958.1 .
    BC117958 mRNA. Translation: AAI17959.1 .
    CCDSi CCDS15137.1. [Q8BWR4-2 ]
    RefSeqi NP_001028463.1. NM_001033291.2. [Q8BWR4-2 ]
    NP_001185502.1. NM_001198573.1. [Q8BWR4-1 ]
    XP_006529542.1. XM_006529479.1. [Q8BWR4-3 ]
    UniGenei Mm.80484.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BWR4. 1 interaction.
    MINTi MINT-4139230.

    Protein family/group databases

    MEROPSi C19.069.

    PTM databases

    PhosphoSitei Q8BWR4.

    Proteomic databases

    PaxDbi Q8BWR4.
    PRIDEi Q8BWR4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040783 ; ENSMUSP00000038533 ; ENSMUSG00000005501 . [Q8BWR4-2 ]
    GeneIDi 227334.
    KEGGi mmu:227334.
    UCSCi uc007bxw.1. mouse. [Q8BWR4-2 ]
    uc007bxx.1. mouse. [Q8BWR4-1 ]
    uc007bxz.1. mouse. [Q8BWR4-4 ]

    Organism-specific databases

    CTDi 55230.
    MGIi MGI:2443184. Usp40.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00740000115066.
    HOVERGENi HBG079302.
    InParanoidi Q8BWR4.
    KOi K11869.
    OMAi VWDLTFD.
    OrthoDBi EOG7R2BHT.
    PhylomeDBi Q8BWR4.
    TreeFami TF106281.

    Miscellaneous databases

    NextBioi 378568.
    PROi Q8BWR4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BWR4.
    CleanExi MM_USP40.
    Genevestigatori Q8BWR4.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1140 AND 967-1235 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo, Liver, Lung and Mammary gland.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6.
      Tissue: Eye.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiUBP40_MOUSE
    AccessioniPrimary (citable) accession number: Q8BWR4
    Secondary accession number(s): Q08EB7
    , Q148V0, Q3TLV1, Q3TMN0, Q3V1R4, Q5EBN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3