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Q8BWP8

- B3GN1_MOUSE

UniProt

Q8BWP8 - B3GN1_MOUSE

Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase

Gene

B3gnt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. Involved in alpha-dystroglycan (DAG1) glycosylation By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. axon guidance Source: MGI
    2. poly-N-acetyllactosamine biosynthetic process Source: Ensembl
    3. protein glycosylation Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_198578. Keratan sulfate biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT49. Glycosyltransferase Family 49.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (EC:2.4.1.149)
    Alternative name(s):
    I-beta-1,3-N-acetylglucosaminyltransferase
    Short name:
    iGnT
    Poly-N-acetyllactosamine extension enzyme
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
    Gene namesi
    Name:B3gnt1
    Synonyms:B3gnt6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1919680. B3gnt1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferasePRO_0000080556Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8BWP8.
    PRIDEiQ8BWP8.

    PTM databases

    PhosphoSiteiQ8BWP8.

    Expressioni

    Gene expression databases

    BgeeiQ8BWP8.
    GenevestigatoriQ8BWP8.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000062016.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini37 – 415379LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3628Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 49 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG325995.
    GeneTreeiENSGT00530000063165.
    HOGENOMiHOG000064521.
    HOVERGENiHBG080885.
    InParanoidiQ8BWP8.
    KOiK00741.
    OMAiMPMSKTE.
    OrthoDBiEOG7PVWPF.
    PhylomeDBiQ8BWP8.
    TreeFamiTF319168.

    Family and domain databases

    InterProiIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
    [Graphical view]
    PANTHERiPTHR12270:SF20. PTHR12270:SF20. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BWP8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP    50
    PSPRSVDQVK SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH 100
    ASVDNLLHLS GLLERWEGPL SVSVFAATKE EAQLATVLAY ALSSHCPEMR 150
    ARVAMHLVCP SRYEAAVPDP REPGEFALLR SCQEVFDKLA RVAQPGINYA 200
    LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL REMLDQSNHW 250
    DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN 300
    YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI 350
    SQACELHVAG FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF 400
    KQELKARYPN SPHRC 415
    Length:415
    Mass (Da):47,384
    Last modified:March 1, 2003 - v1
    Checksum:i7B74BEB591EAA4DC
    GO
    Isoform 2 (identifier: Q8BWP8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         353-379: ACELHVAGFNFEVLNEGFLVHKGFKEA → VLKRERACRTKTGGVSGVKWPLVERRQ
         380-415: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:379
    Mass (Da):43,009
    Checksum:iF1E39CBDF0D3CF27
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1762EF → RV in AAH02191. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei353 – 37927ACELH…GFKEA → VLKRERACRTKTGGVSGVKW PLVERRQ in isoform 2. 1 PublicationVSP_014004Add
    BLAST
    Alternative sequencei380 – 41536Missing in isoform 2. 1 PublicationVSP_014005Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK050347 mRNA. Translation: BAC34203.1.
    AK083857 mRNA. Translation: BAC39041.1.
    AK158899 mRNA. Translation: BAE34720.1.
    BC002191 mRNA. Translation: AAH02191.1.
    BC069927 mRNA. Translation: AAH69927.1.
    CCDSiCCDS29446.1. [Q8BWP8-1]
    RefSeqiNP_780592.1. NM_175383.2. [Q8BWP8-1]
    UniGeneiMm.491160.

    Genome annotation databases

    EnsembliENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379. [Q8BWP8-1]
    GeneIDi108902.
    KEGGimmu:108902.
    UCSCiuc008gbx.1. mouse. [Q8BWP8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK050347 mRNA. Translation: BAC34203.1 .
    AK083857 mRNA. Translation: BAC39041.1 .
    AK158899 mRNA. Translation: BAE34720.1 .
    BC002191 mRNA. Translation: AAH02191.1 .
    BC069927 mRNA. Translation: AAH69927.1 .
    CCDSi CCDS29446.1. [Q8BWP8-1 ]
    RefSeqi NP_780592.1. NM_175383.2. [Q8BWP8-1 ]
    UniGenei Mm.491160.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000062016.

    Protein family/group databases

    CAZyi GT49. Glycosyltransferase Family 49.

    PTM databases

    PhosphoSitei Q8BWP8.

    Proteomic databases

    PaxDbi Q8BWP8.
    PRIDEi Q8BWP8.

    Protocols and materials databases

    DNASUi 108902.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053705 ; ENSMUSP00000062016 ; ENSMUSG00000047379 . [Q8BWP8-1 ]
    GeneIDi 108902.
    KEGGi mmu:108902.
    UCSCi uc008gbx.1. mouse. [Q8BWP8-1 ]

    Organism-specific databases

    CTDi 11041.
    MGIi MGI:1919680. B3gnt1.

    Phylogenomic databases

    eggNOGi NOG325995.
    GeneTreei ENSGT00530000063165.
    HOGENOMi HOG000064521.
    HOVERGENi HBG080885.
    InParanoidi Q8BWP8.
    KOi K00741.
    OMAi MPMSKTE.
    OrthoDBi EOG7PVWPF.
    PhylomeDBi Q8BWP8.
    TreeFami TF319168.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 361433.
    PROi Q8BWP8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BWP8.
    Genevestigatori Q8BWP8.

    Family and domain databases

    InterProi IPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
    [Graphical view ]
    PANTHERi PTHR12270:SF20. PTHR12270:SF20. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Liver, Spinal ganglion and Visual cortex.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II and FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiB3GN1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BWP8
    Secondary accession number(s): Q3TY43, Q8BJH9, Q99LW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3