Reviewed, UniProtKB/Swiss-Prot Q8BWP8 (B3GN1_MOUSE)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
    EC=2.4.1.149
Alternative name(s):
    Poly-N-acetyllactosamine extension enzyme
    I-beta-1,3-N-acetylglucosaminyltransferase
      Short name=iGnT
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

Gene names

Name:	B3gnt1
Synonyms:	B3gnt6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BWP8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BWP8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-379: ACELHVAGFNFEVLNEGFLVHKGFKEA → VLKRERACRTKTGGVSGVKWPLVERRQ
     380-415: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase		PRO_0000080556
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 36	28	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 415	379	Lumenal Potential
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	353 – 379	27	ACELH…GFKEA → VLKRERACRTKTGGVSGVKW PLVERRQ in isoform 2.		VSP_014004
	Alternative sequence	380 – 415	36	Missing in isoform 2.		VSP_014005
Experimental info
	Sequence conflict	175 – 176	2	EF → RV in AAH02191. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7B74BEB591EAA4DC	FASTA	415	47,384
	10 20 30 40 50 60 MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP PSPRSVDQVK 70 80 90 100 110 120 SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 130 140 150 160 170 180 SVSVFAATKE EAQLATVLAY ALSSHCPEMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 190 200 210 220 230 240 SCQEVFDKLA RVAQPGINYA LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL 250 260 270 280 290 300 REMLDQSNHW DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN 310 320 330 340 350 360 YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG 370 380 390 400 410 FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF KQELKARYPN SPHRC
	Isoform 2. Checksum: F1E39CBDF0D3CF27	FASTA	379	43,009
	10 20 30 40 50 60 MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP PSPRSVDQVK 70 80 90 100 110 120 SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 130 140 150 160 170 180 SVSVFAATKE EAQLATVLAY ALSSHCPEMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 190 200 210 220 230 240 SCQEVFDKLA RVAQPGINYA LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL 250 260 270 280 290 300 REMLDQSNHW DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN 310 320 330 340 350 360 YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQVLKRERAC 370 RTKTGGVSGV KWPLVERRQ

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Liver, Spinal ganglion and Visual cortex.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II and FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK050347 mRNA. Translation: BAC34203.1. AK083857 mRNA. Translation: BAC39041.1. AK158899 mRNA. Translation: BAE34720.1. BC002191 mRNA. Translation: AAH02191.1. BC069927 mRNA. Translation: AAH69927.1.
IPI	IPI00226360. IPI00457635.
RefSeq	NP_780592.1.
UniGene	Mm.478323
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BWP8.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49.
Proteomic databases
PRIDE	Q8BWP8.
Genome annotation databases
Ensembl	ENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379; Mus musculus. [Genome view]
GeneID	108902.
KEGG	mmu:108902.
UCSC	uc008gbx.1. mouse.
Organism-specific databases
CTD	108902.
MGI	MGI:1919680. B3gnt1.
Phylogenomic databases
eggNOG	roNOG05871.
HOGENOM	HBG716579.
HOVERGEN	Q8BWP8.
InParanoid	Q8BWP8.
OMA	AKYPDSP.
OrthoDB	EOG973S9Z.
PhylomeDB	Q8BWP8.
Enzyme and pathway databases
BRENDA	2.4.1.149. 244.
Gene expression databases
ArrayExpress	Q8BWP8.
Bgee	Q8BWP8.
Genevestigator	Q8BWP8.
GermOnline	ENSMUSG00000047379. Mus musculus.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	361433.
SOURCE	Search...

Entry information

Entry name

B3GN1_MOUSE

Accession

Primary (citable) accession number: Q8BWP8
Secondary accession number(s): Q3TY43, Q8BJH9, Q99LW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)