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Protein

Beta-1,4-glucuronyltransferase 1

Gene

B3gnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O-mannosyl glycan. Phosphorylated O-mannosyl glycan is a carbohydrate is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed:23217742).2 Publications

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi227 – 2271ManganeseBy similarity
Metal bindingi229 – 2291ManganeseBy similarity

GO - Molecular functioni

  1. glucuronosyltransferase activity Source: UniProtKB
  2. N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: MGI

GO - Biological processi

  1. axon guidance Source: MGI
  2. poly-N-acetyllactosamine biosynthetic process Source: MGI
  3. protein glycosylation Source: MGI
  4. protein O-linked mannosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-glucuronyltransferase 1By similarity (EC:2.4.1.-By similarity)
Alternative name(s):
I-beta-1,3-N-acetylglucosaminyltransferase
Short name:
iGnT
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
Poly-N-acetyllactosamine extension enzyme
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Gene namesi
Name:B3gnt1
Synonyms:B3gnt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1919680. B3gnt1.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein
Note: Localizes near the trans-Golgi apparatus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 3628Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini37 – 415379LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. Golgi apparatus Source: UniProtKB
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Early embryonic lethality.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi155 – 1551M → T: Mild muscular dystrophy phenotype of variable penetrance due to defects in axon guidance. Mislocalization to the endoplasmic reticulum. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Beta-1,4-glucuronyltransferase 1PRO_0000080556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BWP8.
PaxDbiQ8BWP8.
PRIDEiQ8BWP8.

PTM databases

PhosphoSiteiQ8BWP8.

Expressioni

Gene expression databases

BgeeiQ8BWP8.
GenevestigatoriQ8BWP8.

Interactioni

Subunit structurei

Interacts with LARGE and GYLTL1B/LARGE2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062016.

Structurei

3D structure databases

ProteinModelPortaliQ8BWP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 49 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325995.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000064521.
HOVERGENiHBG080885.
InParanoidiQ8BWP8.
KOiK00741.
OMAiMPMSKTE.
OrthoDBiEOG7PVWPF.
PhylomeDBiQ8BWP8.
TreeFamiTF319168.

Family and domain databases

InterProiIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERiPTHR12270:SF20. PTHR12270:SF20. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BWP8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP
60 70 80 90 100
PSPRSVDQVK SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH
110 120 130 140 150
ASVDNLLHLS GLLERWEGPL SVSVFAATKE EAQLATVLAY ALSSHCPEMR
160 170 180 190 200
ARVAMHLVCP SRYEAAVPDP REPGEFALLR SCQEVFDKLA RVAQPGINYA
210 220 230 240 250
LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL REMLDQSNHW
260 270 280 290 300
DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN
310 320 330 340 350
YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI
360 370 380 390 400
SQACELHVAG FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF
410
KQELKARYPN SPHRC
Length:415
Mass (Da):47,384
Last modified:March 1, 2003 - v1
Checksum:i7B74BEB591EAA4DC
GO
Isoform 2 (identifier: Q8BWP8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-379: ACELHVAGFNFEVLNEGFLVHKGFKEA → VLKRERACRTKTGGVSGVKWPLVERRQ
     380-415: Missing.

Note: No experimental confirmation available.

Show »
Length:379
Mass (Da):43,009
Checksum:iF1E39CBDF0D3CF27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1762EF → RV in AAH02191. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei353 – 37927ACELH…GFKEA → VLKRERACRTKTGGVSGVKW PLVERRQ in isoform 2. 1 PublicationVSP_014004Add
BLAST
Alternative sequencei380 – 41536Missing in isoform 2. 1 PublicationVSP_014005Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050347 mRNA. Translation: BAC34203.1.
AK083857 mRNA. Translation: BAC39041.1.
AK158899 mRNA. Translation: BAE34720.1.
BC002191 mRNA. Translation: AAH02191.1.
BC069927 mRNA. Translation: AAH69927.1.
CCDSiCCDS29446.1. [Q8BWP8-1]
RefSeqiNP_780592.1. NM_175383.2. [Q8BWP8-1]
UniGeneiMm.491160.

Genome annotation databases

EnsembliENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379. [Q8BWP8-1]
GeneIDi108902.
KEGGimmu:108902.
UCSCiuc008gbx.1. mouse. [Q8BWP8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050347 mRNA. Translation: BAC34203.1.
AK083857 mRNA. Translation: BAC39041.1.
AK158899 mRNA. Translation: BAE34720.1.
BC002191 mRNA. Translation: AAH02191.1.
BC069927 mRNA. Translation: AAH69927.1.
CCDSiCCDS29446.1. [Q8BWP8-1]
RefSeqiNP_780592.1. NM_175383.2. [Q8BWP8-1]
UniGeneiMm.491160.

3D structure databases

ProteinModelPortaliQ8BWP8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062016.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.

PTM databases

PhosphoSiteiQ8BWP8.

Proteomic databases

MaxQBiQ8BWP8.
PaxDbiQ8BWP8.
PRIDEiQ8BWP8.

Protocols and materials databases

DNASUi108902.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379. [Q8BWP8-1]
GeneIDi108902.
KEGGimmu:108902.
UCSCiuc008gbx.1. mouse. [Q8BWP8-1]

Organism-specific databases

CTDi11041.
MGIiMGI:1919680. B3gnt1.

Phylogenomic databases

eggNOGiNOG325995.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000064521.
HOVERGENiHBG080885.
InParanoidiQ8BWP8.
KOiK00741.
OMAiMPMSKTE.
OrthoDBiEOG7PVWPF.
PhylomeDBiQ8BWP8.
TreeFamiTF319168.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi361433.
PROiQ8BWP8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BWP8.
GenevestigatoriQ8BWP8.

Family and domain databases

InterProiIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERiPTHR12270:SF20. PTHR12270:SF20. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Liver, Spinal ganglion and Visual cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  3. "Dystroglycan organizes axon guidance cue localization and axonal pathfinding."
    Wright K.M., Lyon K.A., Leung H., Leahy D.J., Ma L., Ginty D.D.
    Neuron 76:931-944(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF MET-155.
  4. "The glucuronyltransferase B4GAT1 is required for initiation of LARGE-mediated alpha-dystroglycan functional glycosylation."
    Willer T., Inamori K.I., Venzke D., Harvey C., Morgensen G., Hara Y., Beltran Valero de Bernabe D., Yu L., Wright K.M., Campbell K.P.
    Elife 3:0-0(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PATHWAY.

Entry informationi

Entry nameiB4GA1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWP8
Secondary accession number(s): Q3TY43, Q8BJH9, Q99LW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.