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Q8BWP8

- B3GN1_MOUSE

UniProt

Q8BWP8 - B3GN1_MOUSE

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Protein
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
Gene
B3gnt1, B3gnt6
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. Involved in alpha-dystroglycan (DAG1) glycosylation By similarity.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. axon guidance Source: MGI
  2. poly-N-acetyllactosamine biosynthetic process Source: Ensembl
  3. protein glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (EC:2.4.1.149)
Alternative name(s):
I-beta-1,3-N-acetylglucosaminyltransferase
Short name:
iGnT
Poly-N-acetyllactosamine extension enzyme
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Gene namesi
Name:B3gnt1
Synonyms:B3gnt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1919680. B3gnt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88Cytoplasmic Reviewed prediction
Transmembranei9 – 3628Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini37 – 415379Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
PRO_0000080556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi204 – 2041N-linked (GlcNAc...) Reviewed prediction
Glycosylationi300 – 3001N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BWP8.
PRIDEiQ8BWP8.

PTM databases

PhosphoSiteiQ8BWP8.

Expressioni

Gene expression databases

BgeeiQ8BWP8.
GenevestigatoriQ8BWP8.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062016.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325995.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000064521.
HOVERGENiHBG080885.
InParanoidiQ8BWP8.
KOiK00741.
OMAiMPMSKTE.
OrthoDBiEOG7PVWPF.
PhylomeDBiQ8BWP8.
TreeFamiTF319168.

Family and domain databases

InterProiIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERiPTHR12270:SF20. PTHR12270:SF20. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BWP8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP    50
PSPRSVDQVK SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH 100
ASVDNLLHLS GLLERWEGPL SVSVFAATKE EAQLATVLAY ALSSHCPEMR 150
ARVAMHLVCP SRYEAAVPDP REPGEFALLR SCQEVFDKLA RVAQPGINYA 200
LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL REMLDQSNHW 250
DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN 300
YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI 350
SQACELHVAG FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF 400
KQELKARYPN SPHRC 415
Length:415
Mass (Da):47,384
Last modified:March 1, 2003 - v1
Checksum:i7B74BEB591EAA4DC
GO
Isoform 2 (identifier: Q8BWP8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-379: ACELHVAGFNFEVLNEGFLVHKGFKEA → VLKRERACRTKTGGVSGVKWPLVERRQ
     380-415: Missing.

Note: No experimental confirmation available.

Show »
Length:379
Mass (Da):43,009
Checksum:iF1E39CBDF0D3CF27
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei353 – 37927ACELH…GFKEA → VLKRERACRTKTGGVSGVKW PLVERRQ in isoform 2.
VSP_014004Add
BLAST
Alternative sequencei380 – 41536Missing in isoform 2.
VSP_014005Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1762EF → RV in AAH02191. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK050347 mRNA. Translation: BAC34203.1.
AK083857 mRNA. Translation: BAC39041.1.
AK158899 mRNA. Translation: BAE34720.1.
BC002191 mRNA. Translation: AAH02191.1.
BC069927 mRNA. Translation: AAH69927.1.
CCDSiCCDS29446.1. [Q8BWP8-1]
RefSeqiNP_780592.1. NM_175383.2. [Q8BWP8-1]
UniGeneiMm.491160.

Genome annotation databases

EnsembliENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379. [Q8BWP8-1]
GeneIDi108902.
KEGGimmu:108902.
UCSCiuc008gbx.1. mouse. [Q8BWP8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK050347 mRNA. Translation: BAC34203.1 .
AK083857 mRNA. Translation: BAC39041.1 .
AK158899 mRNA. Translation: BAE34720.1 .
BC002191 mRNA. Translation: AAH02191.1 .
BC069927 mRNA. Translation: AAH69927.1 .
CCDSi CCDS29446.1. [Q8BWP8-1 ]
RefSeqi NP_780592.1. NM_175383.2. [Q8BWP8-1 ]
UniGenei Mm.491160.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000062016.

Protein family/group databases

CAZyi GT49. Glycosyltransferase Family 49.

PTM databases

PhosphoSitei Q8BWP8.

Proteomic databases

PaxDbi Q8BWP8.
PRIDEi Q8BWP8.

Protocols and materials databases

DNASUi 108902.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000053705 ; ENSMUSP00000062016 ; ENSMUSG00000047379 . [Q8BWP8-1 ]
GeneIDi 108902.
KEGGi mmu:108902.
UCSCi uc008gbx.1. mouse. [Q8BWP8-1 ]

Organism-specific databases

CTDi 11041.
MGIi MGI:1919680. B3gnt1.

Phylogenomic databases

eggNOGi NOG325995.
GeneTreei ENSGT00530000063165.
HOGENOMi HOG000064521.
HOVERGENi HBG080885.
InParanoidi Q8BWP8.
KOi K00741.
OMAi MPMSKTE.
OrthoDBi EOG7PVWPF.
PhylomeDBi Q8BWP8.
TreeFami TF319168.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi 361433.
PROi Q8BWP8.
SOURCEi Search...

Gene expression databases

Bgeei Q8BWP8.
Genevestigatori Q8BWP8.

Family and domain databases

InterProi IPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view ]
PANTHERi PTHR12270:SF20. PTHR12270:SF20. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Liver, Spinal ganglion and Visual cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiB3GN1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWP8
Secondary accession number(s): Q3TY43, Q8BJH9, Q99LW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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