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Q8BWP8 (B3GN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase

EC=2.4.1.149
Alternative name(s):
I-beta-1,3-N-acetylglucosaminyltransferase
Short name=iGnT
Poly-N-acetyllactosamine extension enzyme
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Gene names
Name:B3gnt1
Synonyms:B3gnt6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. Involved in alpha-dystroglycan (DAG1) glycosylation By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase 49 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BWP8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BWP8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-379: ACELHVAGFNFEVLNEGFLVHKGFKEA → VLKRERACRTKTGGVSGVKWPLVERRQ
     380-415: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
PRO_0000080556

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 3628Helical; Signal-anchor for type II membrane protein; Potential
Topological domain37 – 415379Lumenal Potential

Amino acid modifications

Glycosylation2041N-linked (GlcNAc...) Potential
Glycosylation3001N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence353 – 37927ACELH…GFKEA → VLKRERACRTKTGGVSGVKW PLVERRQ in isoform 2.
VSP_014004
Alternative sequence380 – 41536Missing in isoform 2.
VSP_014005

Experimental info

Sequence conflict175 – 1762EF → RV in AAH02191. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7B74BEB591EAA4DC

FASTA41547,384
        10         20         30         40         50         60 
MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP PSPRSVDQVK 

        70         80         90        100        110        120 
SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 

       130        140        150        160        170        180 
SVSVFAATKE EAQLATVLAY ALSSHCPEMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 

       190        200        210        220        230        240 
SCQEVFDKLA RVAQPGINYA LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL 

       250        260        270        280        290        300 
REMLDQSNHW DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN 

       310        320        330        340        350        360 
YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG 

       370        380        390        400        410 
FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF KQELKARYPN SPHRC 

« Hide

Isoform 2 [UniParc].

Checksum: F1E39CBDF0D3CF27
Show »

FASTA37943,009

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Liver, Spinal ganglion and Visual cortex.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II and FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK050347 mRNA. Translation: BAC34203.1.
AK083857 mRNA. Translation: BAC39041.1.
AK158899 mRNA. Translation: BAE34720.1.
BC002191 mRNA. Translation: AAH02191.1.
BC069927 mRNA. Translation: AAH69927.1.
RefSeqNP_780592.1. NM_175383.2.
UniGeneMm.491160.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000062016.

Protein family/group databases

CAZyGT49. Glycosyltransferase Family 49.

PTM databases

PhosphoSiteQ8BWP8.

Proteomic databases

PaxDbQ8BWP8.
PRIDEQ8BWP8.

Protocols and materials databases

DNASU108902.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053705; ENSMUSP00000062016; ENSMUSG00000047379. [Q8BWP8-1]
GeneID108902.
KEGGmmu:108902.
UCSCuc008gbx.1. mouse. [Q8BWP8-1]

Organism-specific databases

CTD11041.
MGIMGI:1919680. B3gnt1.

Phylogenomic databases

eggNOGNOG325995.
GeneTreeENSGT00530000063165.
HOGENOMHOG000064521.
HOVERGENHBG080885.
InParanoidQ8BWP8.
KOK00741.
OMAMPMSKTE.
OrthoDBEOG7PVWPF.
PhylomeDBQ8BWP8.
TreeFamTF319168.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ8BWP8.
GenevestigatorQ8BWP8.

Family and domain databases

InterProIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERPTHR12270:SF3. PTHR12270:SF3. 1 hit.
ProtoNetSearch...

Other

NextBio361433.
PROQ8BWP8.
SOURCESearch...

Entry information

Entry nameB3GN1_MOUSE
AccessionPrimary (citable) accession number: Q8BWP8
Secondary accession number(s): Q3TY43, Q8BJH9, Q99LW7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot