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Protein

Acyl-coenzyme A thioesterase 4

Gene

Acot4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.By similarity

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Charge relay systemBy similarity1
Active sitei326Charge relay systemBy similarity1
Active sitei360Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-75105. Fatty Acyl-CoA Biosynthesis.

Protein family/group databases

ESTHERimouse-acot4. Acyl-CoA_Thioesterase.
MEROPSiS09.A60.

Chemistry databases

SwissLipidsiSLP:000000593.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 4 (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 4
Alternative name(s):
PTE-2b
Peroxisomal acyl coenzyme A thioester hydrolase Ib
Peroxisomal long-chain acyl-CoA thioesterase Ib
Short name:
PTE-Ib
Gene namesi
Name:Acot4
Synonyms:Pte1b, Pte2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2159621. Acot4.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021501 – 421Acyl-coenzyme A thioesterase 4Add BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei313N6-succinyllysineCombined sources1
Modified residuei408N6-succinyllysineBy similarity1
Modified residuei418PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BWN8.
PaxDbiQ8BWN8.
PRIDEiQ8BWN8.

PTM databases

iPTMnetiQ8BWN8.
PhosphoSitePlusiQ8BWN8.

Expressioni

Tissue specificityi

Expressed in liver, kidney, intestine, adrenal gland and white and brown tissues. Not detected in other tissues.1 Publication

Inductioni

In the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours.1 Publication

Gene expression databases

BgeeiENSMUSG00000052392.
CleanExiMM_ACOT4.
ExpressionAtlasiQ8BWN8. baseline and differential.
GenevisibleiQ8BWN8. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BWN8. 3 interactors.
MINTiMINT-1850016.
STRINGi10090.ENSMUSP00000021652.

Structurei

3D structure databases

ProteinModelPortaliQ8BWN8.
SMRiQ8BWN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi419 – 421Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
GeneTreeiENSGT00390000001046.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiQ8BWN8.
KOiK01068.
OMAiCCWNEPV.
OrthoDBiEOG091G08KU.
PhylomeDBiQ8BWN8.
TreeFamiTF314911.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8BWN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATLSVEPT GRSCWDEPLS IAVRGLAPEQ PVTLRSVLRD EKGALFRAHA
60 70 80 90 100
RYRADSHGEL DLARVPALGG SFSGLEPMGL LWAMEPDRPF WRLIKRDVQT
110 120 130 140 150
PFLVELEVLD GHEPDGGRRL ARTVHERHFM APGVRRVPVR EGRVRATLFL
160 170 180 190 200
PPGQGPFPGI IDVYGVGGGL LEYRAGLVAG HGFATLALAF YDFEDLPKEL
210 220 230 240 250
NVIEVDYFEE AVRYMLRHPK VKGPDIGLLG LSLGADVCLI MASFLNNVSA
260 270 280 290 300
TVSINGSAFS GNRHIKYKQT MIPPLGHDLR RMKVAFSGIL DIVDIRNDAV
310 320 330 340 350
GGCENPSMIP IEKAKGPILF VAGQDDHCWR SELYTQIASD RLQAHGKERP
360 370 380 390 400
QVLSYPGTGH YIEPPYFPMC PASLHKIVNE AVIWGGEVKA HSKAQIDAWK
410 420
QILFFFGKHL GSTHSRASCR L
Length:421
Mass (Da):46,481
Last modified:March 1, 2003 - v1
Checksum:i93DB75F0191D49F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87D → N in BAC32814 (PubMed:16141072).Curated1
Sequence conflicti136R → G in AAF13872 (PubMed:10567408).Curated1
Sequence conflicti215M → K in BAC38060 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180801, AF180799, AF180800 Genomic DNA. Translation: AAF13872.1.
AK046630 mRNA. Translation: BAC32814.1.
AK050420 mRNA. Translation: BAC34246.1.
AK080883 mRNA. Translation: BAC38060.1.
CCDSiCCDS26035.1.
RefSeqiNP_599008.3. NM_134247.3.
UniGeneiMm.482217.

Genome annotation databases

EnsembliENSMUST00000021652; ENSMUSP00000021652; ENSMUSG00000052392.
GeneIDi171282.
KEGGimmu:171282.
UCSCiuc007oed.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180801, AF180799, AF180800 Genomic DNA. Translation: AAF13872.1.
AK046630 mRNA. Translation: BAC32814.1.
AK050420 mRNA. Translation: BAC34246.1.
AK080883 mRNA. Translation: BAC38060.1.
CCDSiCCDS26035.1.
RefSeqiNP_599008.3. NM_134247.3.
UniGeneiMm.482217.

3D structure databases

ProteinModelPortaliQ8BWN8.
SMRiQ8BWN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BWN8. 3 interactors.
MINTiMINT-1850016.
STRINGi10090.ENSMUSP00000021652.

Chemistry databases

SwissLipidsiSLP:000000593.

Protein family/group databases

ESTHERimouse-acot4. Acyl-CoA_Thioesterase.
MEROPSiS09.A60.

PTM databases

iPTMnetiQ8BWN8.
PhosphoSitePlusiQ8BWN8.

Proteomic databases

MaxQBiQ8BWN8.
PaxDbiQ8BWN8.
PRIDEiQ8BWN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021652; ENSMUSP00000021652; ENSMUSG00000052392.
GeneIDi171282.
KEGGimmu:171282.
UCSCiuc007oed.1. mouse.

Organism-specific databases

CTDi122970.
MGIiMGI:2159621. Acot4.

Phylogenomic databases

eggNOGiENOG410II3X. Eukaryota.
COG1073. LUCA.
GeneTreeiENSGT00390000001046.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiQ8BWN8.
KOiK01068.
OMAiCCWNEPV.
OrthoDBiEOG091G08KU.
PhylomeDBiQ8BWN8.
TreeFamiTF314911.

Enzyme and pathway databases

ReactomeiR-MMU-75105. Fatty Acyl-CoA Biosynthesis.

Miscellaneous databases

PROiQ8BWN8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052392.
CleanExiMM_ACOT4.
ExpressionAtlasiQ8BWN8. baseline and differential.
GenevisibleiQ8BWN8. MM.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiACOT4_MOUSE
AccessioniPrimary (citable) accession number: Q8BWN8
Secondary accession number(s): Q8BJQ1, Q8BL20, Q9QYR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.