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Protein

Calcium release-activated calcium channel protein 1

Gene

Orai1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.By similarity

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • calcium ion import Source: MGI
  • mammary gland epithelium development Source: MGI
  • positive regulation of calcium ion transport Source: UniProtKB
  • regulation of calcium ion transport Source: MGI
  • store-operated calcium entry Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai-1
Transmembrane protein 142A
Gene namesi
Name:Orai1
Synonyms:Cracm1, Tmem142a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1925542. Orai1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicleautophagosome By similarity

  • Note: Colocalizes with UBQLN1 in the autophagosome.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 89CytoplasmicBy similarityAdd BLAST89
Transmembranei90 – 107HelicalSequence analysisAdd BLAST18
Topological domaini108 – 121ExtracellularSequence analysisAdd BLAST14
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 175CytoplasmicSequence analysisAdd BLAST33
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 237ExtracellularSequence analysisAdd BLAST41
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 304CytoplasmicBy similarityAdd BLAST46

GO - Cellular componenti

  • autophagosome Source: UniProtKB-SubCell
  • basolateral plasma membrane Source: MGI
  • cytoplasmic vesicle Source: UniProtKB-KW
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002343821 – 304Calcium release-activated calcium channel protein 1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi225N-linked (GlcNAc...)Sequence analysis1
Modified residuei298PhosphothreonineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BWG9.
MaxQBiQ8BWG9.
PaxDbiQ8BWG9.
PRIDEiQ8BWG9.

PTM databases

iPTMnetiQ8BWG9.
PhosphoSitePlusiQ8BWG9.
SwissPalmiQ8BWG9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049686.
ExpressionAtlasiQ8BWG9. baseline and differential.
GenevisibleiQ8BWG9. MM.

Interactioni

Subunit structurei

Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SLC35G1. Interacts with UBQLN1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058169.

Structurei

3D structure databases

ProteinModelPortaliQ8BWG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 92Interaction with STIM1By similarityAdd BLAST21
Regioni275 – 295Interaction with STIM1By similarityAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 50Poly-Pro8

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
GeneTreeiENSGT00390000015354.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ8BWG9.
KOiK16056.
OMAiDAEHDYP.
OrthoDBiEOG091G0E4P.
PhylomeDBiQ8BWG9.
TreeFamiTF313576.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 2 hits.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BWG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP
60 70 80 90 100
AVSYPDWIGQ SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG
110 120 130 140 150
FAMVAMVEVQ LDTDHDYPPG LLIVFSACTT VLVAVHLFAL MISTCILPNI
160 170 180 190 200
EAVSNVHNLN SVKESPHERM HRHIELAWAF STVIGTLLFL AEVVLLCWVK
210 220 230 240 250
FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI ASTAIMVPCG
260 270 280 290 300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG

THYA
Length:304
Mass (Da):33,062
Last modified:March 1, 2003 - v1
Checksum:i9743395B63D2E41F
GO

Sequence cautioni

The sequence AAH23149 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16L → F in AAH23149 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA. Translation: BAC35045.1.
AK085755 mRNA. Translation: BAC39533.1.
BC023149 mRNA. Translation: AAH23149.1. Different initiation.
CCDSiCCDS39261.1.
RefSeqiNP_780632.1. NM_175423.3.
UniGeneiMm.218582.

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
GeneIDi109305.
KEGGimmu:109305.
UCSCiuc008zmy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA. Translation: BAC35045.1.
AK085755 mRNA. Translation: BAC39533.1.
BC023149 mRNA. Translation: AAH23149.1. Different initiation.
CCDSiCCDS39261.1.
RefSeqiNP_780632.1. NM_175423.3.
UniGeneiMm.218582.

3D structure databases

ProteinModelPortaliQ8BWG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058169.

PTM databases

iPTMnetiQ8BWG9.
PhosphoSitePlusiQ8BWG9.
SwissPalmiQ8BWG9.

Proteomic databases

EPDiQ8BWG9.
MaxQBiQ8BWG9.
PaxDbiQ8BWG9.
PRIDEiQ8BWG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
GeneIDi109305.
KEGGimmu:109305.
UCSCiuc008zmy.1. mouse.

Organism-specific databases

CTDi84876.
MGIiMGI:1925542. Orai1.

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
GeneTreeiENSGT00390000015354.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ8BWG9.
KOiK16056.
OMAiDAEHDYP.
OrthoDBiEOG091G0E4P.
PhylomeDBiQ8BWG9.
TreeFamiTF313576.

Miscellaneous databases

ChiTaRSiOrai1. mouse.
PROiQ8BWG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049686.
ExpressionAtlasiQ8BWG9. baseline and differential.
GenevisibleiQ8BWG9. MM.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 2 hits.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRCM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWG9
Secondary accession number(s): Q8BUD4, Q8R586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.