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Protein

Calcium release-activated calcium channel protein 1

Gene

Orai1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Enzyme and pathway databases

ReactomeiREACT_271639. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_311877. Elevation of cytosolic Ca2+ levels.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai-1
Transmembrane protein 142A
Gene namesi
Name:Orai1
Synonyms:Cracm1, Tmem142a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1925542. Orai1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicleautophagosome By similarity

  • Note: Colocalizes with UBQLN1 in the autophagosome.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8989CytoplasmicBy similarityAdd
BLAST
Transmembranei90 – 10718HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 12114ExtracellularSequence AnalysisAdd
BLAST
Transmembranei122 – 14221HelicalSequence AnalysisAdd
BLAST
Topological domaini143 – 17533CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Topological domaini197 – 23741ExtracellularSequence AnalysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence AnalysisAdd
BLAST
Topological domaini259 – 30446CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • autophagosome Source: MGI
  • cytoplasmic vesicle Source: UniProtKB-KW
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Calcium release-activated calcium channel protein 1PRO_0000234382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
Modified residuei298 – 2981PhosphothreonineBy similarity

Post-translational modificationi

N-glycosylated.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8BWG9.
PaxDbiQ8BWG9.
PRIDEiQ8BWG9.

PTM databases

PhosphoSiteiQ8BWG9.

Expressioni

Gene expression databases

BgeeiQ8BWG9.
ExpressionAtlasiQ8BWG9. baseline and differential.
GenevisibleiQ8BWG9. MM.

Interactioni

Subunit structurei

Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SLC35G1. Interacts with UBQLN1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058169.

Structurei

3D structure databases

ProteinModelPortaliQ8BWG9.
SMRiQ8BWG9. Positions 76-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 9221Interaction with STIM1By similarityAdd
BLAST
Regioni275 – 29521Interaction with STIM1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 508Poly-Pro

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263788.
GeneTreeiENSGT00390000015354.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ8BWG9.
KOiK16056.
OMAiITSTSIM.
OrthoDBiEOG7G1V73.
PhylomeDBiQ8BWG9.
TreeFamiTF313576.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 1 hit.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BWG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP
60 70 80 90 100
AVSYPDWIGQ SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG
110 120 130 140 150
FAMVAMVEVQ LDTDHDYPPG LLIVFSACTT VLVAVHLFAL MISTCILPNI
160 170 180 190 200
EAVSNVHNLN SVKESPHERM HRHIELAWAF STVIGTLLFL AEVVLLCWVK
210 220 230 240 250
FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI ASTAIMVPCG
260 270 280 290 300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG

THYA
Length:304
Mass (Da):33,062
Last modified:March 1, 2003 - v1
Checksum:i9743395B63D2E41F
GO

Sequence cautioni

The sequence AAH23149.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161L → F in AAH23149 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA. Translation: BAC35045.1.
AK085755 mRNA. Translation: BAC39533.1.
BC023149 mRNA. Translation: AAH23149.1. Different initiation.
CCDSiCCDS39261.1.
RefSeqiNP_780632.1. NM_175423.3.
UniGeneiMm.218582.

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
GeneIDi109305.
KEGGimmu:109305.
UCSCiuc008zmy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052573 mRNA. Translation: BAC35045.1.
AK085755 mRNA. Translation: BAC39533.1.
BC023149 mRNA. Translation: AAH23149.1. Different initiation.
CCDSiCCDS39261.1.
RefSeqiNP_780632.1. NM_175423.3.
UniGeneiMm.218582.

3D structure databases

ProteinModelPortaliQ8BWG9.
SMRiQ8BWG9. Positions 76-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058169.

PTM databases

PhosphoSiteiQ8BWG9.

Proteomic databases

MaxQBiQ8BWG9.
PaxDbiQ8BWG9.
PRIDEiQ8BWG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
GeneIDi109305.
KEGGimmu:109305.
UCSCiuc008zmy.1. mouse.

Organism-specific databases

CTDi84876.
MGIiMGI:1925542. Orai1.

Phylogenomic databases

eggNOGiNOG263788.
GeneTreeiENSGT00390000015354.
HOGENOMiHOG000246925.
HOVERGENiHBG081343.
InParanoidiQ8BWG9.
KOiK16056.
OMAiITSTSIM.
OrthoDBiEOG7G1V73.
PhylomeDBiQ8BWG9.
TreeFamiTF313576.

Enzyme and pathway databases

ReactomeiREACT_271639. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_311877. Elevation of cytosolic Ca2+ levels.

Miscellaneous databases

ChiTaRSiOrai1. mouse.
NextBioi361899.
PROiQ8BWG9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BWG9.
ExpressionAtlasiQ8BWG9. baseline and differential.
GenevisibleiQ8BWG9. MM.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 1 hit.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland and Stomach.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-304.
    Strain: Czech II.
    Tissue: Mammary gland.

Entry informationi

Entry nameiCRCM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BWG9
Secondary accession number(s): Q8BUD4, Q8R586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.