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Q8BWG9

- CRCM1_MOUSE

UniProt

Q8BWG9 - CRCM1_MOUSE

Protein

Calcium release-activated calcium channel protein 1

Gene

Orai1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. store-operated calcium channel activity Source: UniProtKB

    GO - Biological processi

    1. immune system process Source: UniProtKB-KW
    2. positive regulation of calcium ion transport Source: UniProtKB
    3. store-operated calcium entry Source: MGI

    Keywords - Molecular functioni

    Calcium channel, Ion channel

    Keywords - Biological processi

    Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Calmodulin-binding

    Enzyme and pathway databases

    ReactomeiREACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_227667. Elevation of cytosolic Ca2+ levels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium release-activated calcium channel protein 1
    Alternative name(s):
    Protein orai-1
    Transmembrane protein 142A
    Gene namesi
    Name:Orai1
    Synonyms:Cracm1, Tmem142a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1925542. Orai1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. membrane Source: MGI
    3. protein complex Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304Calcium release-activated calcium channel protein 1PRO_0000234382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
    Modified residuei298 – 2981PhosphothreonineBy similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BWG9.
    PRIDEiQ8BWG9.

    PTM databases

    PhosphoSiteiQ8BWG9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BWG9.
    BgeeiQ8BWG9.
    GenevestigatoriQ8BWG9.

    Interactioni

    Subunit structurei

    Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations By similarity. Interacts with SLC35G1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BWG9.
    SMRiQ8BWG9. Positions 76-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8989CytoplasmicBy similarityAdd
    BLAST
    Topological domaini108 – 12114ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini143 – 17533CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini197 – 23741ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini259 – 30446CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei90 – 10718HelicalSequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 9221Interaction with STIM1By similarityAdd
    BLAST
    Regioni275 – 29521Interaction with STIM1By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi43 – 508Poly-Pro

    Sequence similaritiesi

    Belongs to the Orai family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263788.
    GeneTreeiENSGT00390000015354.
    HOGENOMiHOG000246925.
    HOVERGENiHBG081343.
    InParanoidiQ8BWG9.
    KOiK16056.
    OMAiITSTSIM.
    OrthoDBiEOG7G1V73.
    PhylomeDBiQ8BWG9.
    TreeFamiTF313576.

    Family and domain databases

    InterProiIPR012446. CRAC_channel.
    [Graphical view]
    PfamiPF07856. Orai-1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BWG9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP    50
    AVSYPDWIGQ SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG 100
    FAMVAMVEVQ LDTDHDYPPG LLIVFSACTT VLVAVHLFAL MISTCILPNI 150
    EAVSNVHNLN SVKESPHERM HRHIELAWAF STVIGTLLFL AEVVLLCWVK 200
    FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI ASTAIMVPCG 250
    LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG 300
    THYA 304
    Length:304
    Mass (Da):33,062
    Last modified:March 1, 2003 - v1
    Checksum:i9743395B63D2E41F
    GO

    Sequence cautioni

    The sequence AAH23149.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161L → F in AAH23149. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052573 mRNA. Translation: BAC35045.1.
    AK085755 mRNA. Translation: BAC39533.1.
    BC023149 mRNA. Translation: AAH23149.1. Different initiation.
    CCDSiCCDS39261.1.
    RefSeqiNP_780632.1. NM_175423.3.
    UniGeneiMm.218582.

    Genome annotation databases

    EnsembliENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
    ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
    GeneIDi109305.
    KEGGimmu:109305.
    UCSCiuc008zmy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052573 mRNA. Translation: BAC35045.1 .
    AK085755 mRNA. Translation: BAC39533.1 .
    BC023149 mRNA. Translation: AAH23149.1 . Different initiation.
    CCDSi CCDS39261.1.
    RefSeqi NP_780632.1. NM_175423.3.
    UniGenei Mm.218582.

    3D structure databases

    ProteinModelPortali Q8BWG9.
    SMRi Q8BWG9. Positions 76-295.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BWG9.

    Proteomic databases

    PaxDbi Q8BWG9.
    PRIDEi Q8BWG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000051016 ; ENSMUSP00000058169 ; ENSMUSG00000049686 .
    ENSMUST00000121652 ; ENSMUSP00000113097 ; ENSMUSG00000049686 .
    GeneIDi 109305.
    KEGGi mmu:109305.
    UCSCi uc008zmy.1. mouse.

    Organism-specific databases

    CTDi 84876.
    MGIi MGI:1925542. Orai1.

    Phylogenomic databases

    eggNOGi NOG263788.
    GeneTreei ENSGT00390000015354.
    HOGENOMi HOG000246925.
    HOVERGENi HBG081343.
    InParanoidi Q8BWG9.
    KOi K16056.
    OMAi ITSTSIM.
    OrthoDBi EOG7G1V73.
    PhylomeDBi Q8BWG9.
    TreeFami TF313576.

    Enzyme and pathway databases

    Reactomei REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_227667. Elevation of cytosolic Ca2+ levels.

    Miscellaneous databases

    ChiTaRSi ORAI1. mouse.
    NextBioi 361899.
    PROi Q8BWG9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BWG9.
    Bgeei Q8BWG9.
    Genevestigatori Q8BWG9.

    Family and domain databases

    InterProi IPR012446. CRAC_channel.
    [Graphical view ]
    Pfami PF07856. Orai-1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland and Stomach.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-304.
      Strain: Czech II.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiCRCM1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BWG9
    Secondary accession number(s): Q8BUD4, Q8R586
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3