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Q8BWG9 (CRCM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai-1
Transmembrane protein 142A
Gene names
Name:Orai1
Synonyms:Cracm1, Tmem142a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT By similarity.

Subunit structure

Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca2+. Forms a complex with EFCAB4B/CRACR2A and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the Orai family.

Sequence caution

The sequence AAH23149.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Calcium release-activated calcium channel protein 1
PRO_0000234382

Regions

Topological domain1 – 8989Cytoplasmic By similarity
Transmembrane90 – 10718Helical; Potential
Topological domain108 – 12114Extracellular Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 17533Cytoplasmic Potential
Transmembrane176 – 19621Helical; Potential
Topological domain197 – 23741Extracellular Potential
Transmembrane238 – 25821Helical; Potential
Topological domain259 – 30446Cytoplasmic By similarity
Region72 – 9221Interaction with STIM1 By similarity
Region275 – 29521Interaction with STIM1 By similarity
Compositional bias43 – 508Poly-Pro

Amino acid modifications

Modified residue2981Phosphothreonine By similarity
Glycosylation2251N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict161L → F in AAH23149. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8BWG9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 9743395B63D2E41F

FASTA30433,062
        10         20         30         40         50         60 
MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP AVSYPDWIGQ 

        70         80         90        100        110        120 
SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG FAMVAMVEVQ LDTDHDYPPG 

       130        140        150        160        170        180 
LLIVFSACTT VLVAVHLFAL MISTCILPNI EAVSNVHNLN SVKESPHERM HRHIELAWAF 

       190        200        210        220        230        240 
STVIGTLLFL AEVVLLCWVK FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI 

       250        260        270        280        290        300 
ASTAIMVPCG LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG 


THYA 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland and Stomach.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-304.
Strain: Czech II.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK052573 mRNA. Translation: BAC35045.1.
AK085755 mRNA. Translation: BAC39533.1.
BC023149 mRNA. Translation: AAH23149.1. Different initiation.
RefSeqNP_780632.1. NM_175423.3.
UniGeneMm.218582.

3D structure databases

ProteinModelPortalQ8BWG9.
SMRQ8BWG9. Positions 76-295.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BWG9.

Proteomic databases

PaxDbQ8BWG9.
PRIDEQ8BWG9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000051016; ENSMUSP00000058169; ENSMUSG00000049686.
ENSMUST00000121652; ENSMUSP00000113097; ENSMUSG00000049686.
GeneID109305.
KEGGmmu:109305.
UCSCuc008zmy.1. mouse.

Organism-specific databases

CTD84876.
MGIMGI:1925542. Orai1.

Phylogenomic databases

eggNOGNOG263788.
GeneTreeENSGT00390000015354.
HOGENOMHOG000246925.
HOVERGENHBG081343.
InParanoidQ8BWG9.
KOK16056.
OMAMHPEPAP.
OrthoDBEOG7G1V73.
PhylomeDBQ8BWG9.
TreeFamTF313576.

Gene expression databases

ArrayExpressQ8BWG9.
BgeeQ8BWG9.
GenevestigatorQ8BWG9.

Family and domain databases

InterProIPR012446. CRAC_channel.
[Graphical view]
PfamPF07856. Orai-1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSORAI1. mouse.
NextBio361899.
PROQ8BWG9.
SOURCESearch...

Entry information

Entry nameCRCM1_MOUSE
AccessionPrimary (citable) accession number: Q8BWG9
Secondary accession number(s): Q8BUD4, Q8R586
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot