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Reviewed, UniProtKB/Swiss-Prot Q8BW96 (KCC1D_MOUSE)

Last modified November 3, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Calcium/calmodulin-dependent protein kinase type 1D
    EC=2.7.11.17
Alternative name(s):
    CaM kinase ID
    CaM kinase I delta
      Short name=CaM-KI delta
      Short name=CaMKI-delta
      Short name=CaMKI delta
    Camk1D
    CamKI-like protein kinase
      Short name=mCKLiK
      Short name=CKLiK
Gene names
Name: Camk1d
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 By similarity. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1 By similarity. Isoform 1 but not isoform 2 activates CREB1.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by Ca2+/calmodulin. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. At least in part, also activated by CAMKK1 in a calcium-independent manner By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly cytoplasmic By similarity. Nuclear upon activation By similarity.

Tissue specificity

Expressed ubiquitously with high levels in brain and low levels in kidney. Isoform 2 is highly expressed in brain compared to other tissues. In hematopoietic cell lines predominant expression was detected in T and EC cells. Ref.1

Induction

Down-regulated upon cholesterol-rich diet. Ref.2

Domain

The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BW96-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BW96-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     333-385: ASVSSNLSLA...TVTTGHTGSK → VWHLPRSVVSFLLRRGSQESELRGDPQPPL
Note: Inactive. Does not activate CREB1.
Isoform 3 (identifier: Q8BW96-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MARENGESSSSWKKQAEDIKKIFEFKETLGT → MAEFVSWSCLNFRWSWIKGSRNS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Calcium/calmodulin-dependent protein kinase type 1D
PRO_0000086083

Regions

Domain23 – 279257Protein kinase
Nucleotide binding29 – 379ATP By similarity
Region279 – 31941Autoinhibitory domain By similarity
Region299 – 32022Calmodulin-binding By similarity
Motif318 – 3247Nuclear export signal By similarity

Sites

Active site1441Proton acceptor By similarity
Binding site521ATP By similarity

Amino acid modifications

Modified residue1221Phosphoserine By similarity
Modified residue1801Phosphothreonine; by CaMKK1, CaMKK2 and autocatalysis By similarity

Natural variations

Alternative sequence1 – 3131MAREN…ETLGT → MAEFVSWSCLNFRWSWIKGS RNS in isoform 3.
VSP_012136
Alternative sequence333 – 38553ASVSS…HTGSK → VWHLPRSVVSFLLRRGSQES ELRGDPQPPL in isoform 2.
VSP_012137

Experimental info

Sequence conflict301G → Q in BAC35295. Ref.3
Sequence conflict3271S → N in BAC35295. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 320EB0D3D5670055

FASTA38542,919
        10         20         30         40         50         60 
MARENGESSS SWKKQAEDIK KIFEFKETLG TGAFSEVVLA EEKATGKLFA VKCIPKKALK 

        70         80         90        100        110        120 
GKESSIENEI AVLRKIKHEN IVALEDIYES PNHLYLVMQL VSGGELFDRI VEKGFYTEKD 

       130        140        150        160        170        180 
ASTLIRQVLD AVYYLHRMGI VHRDLKPENL LYYSQDEESK IMISDFGLSK MEGKGDVMST 

       190        200        210        220        230        240 
ACGTPGYVAP EVLAQKPYSK AVDCWSIGVI AYILLCGYPP FYDENDSKLF EQILKAEYEF 

       250        260        270        280        290        300 
DSPYWDDISD SAKDFIRNLM EKDPNKRYTC EQAARHPWIA GDTALSKNIH ESVSAQIRKN 

       310        320        330        340        350        360 
FAKSKWRQAF NATAVVRHMR RLQLGSSLDS SNASVSSNLS LASQKDCLAP STLCSFLSSS 

       370        380 
SGVAGVGAER RPRPTTVTTG HTGSK 

« Hide

Isoform 2 (Beta).

Checksum: F3D0573864293BF5
Show »

FASTA36241,110
Isoform 3.

Checksum: BAC17016D8CF9F91
Show »

FASTA37742,135

References

« Hide 'large scale' references
[1]"Effects of PU.1-induced mouse calcium-calmodulin-dependent kinase I-like kinase (CKLiK) on apoptosis of murine erythroleukemia cells."
Yamada T., Suzuki M., Satoh H., Kihara-Negishi F., Nakano H., Oikawa T.
Exp. Cell Res. 294:39-50(2004) [PubMed: 14980499] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
[2]"Novel putative SREBP and LXR target genes identified by microarray analysis in liver of cholesterol-fed mice."
Maxwell K.N., Soccio R.E., Duncan E.M., Sehayek E., Breslow J.L.
J. Lipid Res. 44:2109-2119(2003) [PubMed: 12897189] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INDUCTION.
Strain: C57BL/6.
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Brain and Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

AY273822 mRNA. Translation: AAP31673.1.
AK052401 mRNA. Translation: BAC34975.1.
AK053173 mRNA. Translation: BAC35295.1.
AK147616 mRNA. Translation: BAE28027.1.
AK154434 mRNA. Translation: BAE32584.1.
AK170777 mRNA. Translation: BAE42022.1.
IPIIPI00226188.
IPI00403595.
IPI00480395.
RefSeqNP_796317.2.
UniGeneMm.191949

3D structure databases

HSSPHSSP built from PDB template 1A06 based on UniProtKB Q63450.
SMRQ8BW96. Positions 11-313.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8BW96.

PTM databases

PhosphoSiteQ8BW96.

Proteomic databases

PRIDEQ8BW96.

Genome annotation databases

EnsemblENSMUST00000044009; ENSMUSP00000037028; ENSMUSG00000039145; Mus musculus. [Genome view]
ENSMUST00000114987; ENSMUSP00000110638; ENSMUSG00000039145; Mus musculus. [Genome view]
GeneID227541.
KEGGmmu:227541.
NMPDRfig|10090.3.peg.5372.
UCSCuc008ifp.1. mouse.
uc008ifr.1. mouse.

Organism-specific databases

CTD227541.
MGIMGI:2442190. Camk1d.

Phylogenomic databases

HOGENOMQ8BW96.
HOVERGENQ8BW96.
OMAVTTVHSG.

Enzyme and pathway databases

BRENDA2.7.11.17. 244.

Gene expression databases

ArrayExpressQ8BW96.
BgeeQ8BW96.
CleanExMM_CAMK1D.
GenevestigatorQ8BW96.
GermOnlineENSMUSG00000039145. Mus musculus.

Family and domain databases

InterProIPR015734. Ca/calmodulin-dep_kinase_1.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22982:SF34. CaMKI. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio378629.
SOURCESearch...

Entry information

Entry nameKCC1D_MOUSE
AccessionPrimary (citable) accession number: Q8BW96
Secondary accession number(s): Q3U450, Q80W64, Q8BWI7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents