ID   C5AR2_MOUSE             Reviewed;         344 AA.
AC   Q8BW93; B2RQI1; Q3TVM8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   24-JAN-2024, entry version 143.
DE   RecName: Full=C5a anaphylatoxin chemotactic receptor 2;
DE   AltName: Full=Complement component 5a receptor 2;
DE   AltName: Full=G-protein coupled receptor 77;
GN   Name=C5ar2; Synonyms=C5l2, Gpr77;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Lung;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=15833747; DOI=10.1074/jbc.m406921200;
RA   Kalant D., MacLaren R., Cui W., Samanta R., Monk P.N., Laporte S.A.,
RA   Cianflone K.;
RT   "C5L2 is a functional receptor for acylation-stimulating protein.";
RL   J. Biol. Chem. 280:23936-23944(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
CC   -!- FUNCTION: Receptor for the chemotactic and inflammatory C3a, C4a and
CC       C5a anaphylatoxin peptides and also for their dearginated forms
CC       ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to
CC       G(i)-mediated signaling pathways (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with C3 (the anaphylatoxin peptide C3a and the
CC       adipogenic hormone ASP); the interaction occurs with higher affinity
CC       for ASP, enhancing the phosphorylation and activation of GPR77,
CC       recruitment of ARRB2 to the cell surface and endocytosis of GRP77.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Highly expressed in liver and spleen. Lower levels
CC       in intestine, brain and kidney. Also expressed in adipose tissues with
CC       highest levels in gonadal and ingual fat depots. Lower levels in brown
CC       tissue. {ECO:0000269|PubMed:15833747}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE35590.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AK053187; BAC35303.1; -; mRNA.
DR   EMBL; AK160050; BAE35590.1; ALT_INIT; mRNA.
DR   EMBL; BC137941; AAI37942.1; -; mRNA.
DR   EMBL; BC145834; AAI45835.1; -; mRNA.
DR   CCDS; CCDS52036.2; -.
DR   RefSeq; NP_001139477.1; NM_001146005.1.
DR   RefSeq; NP_795886.2; NM_176912.4.
DR   RefSeq; XP_006540112.2; XM_006540049.2.
DR   RefSeq; XP_006540115.1; XM_006540052.3.
DR   RefSeq; XP_006540116.1; XM_006540053.3.
DR   RefSeq; XP_006540117.1; XM_006540054.3.
DR   RefSeq; XP_006540118.1; XM_006540055.3.
DR   RefSeq; XP_006540119.1; XM_006540056.2.
DR   AlphaFoldDB; Q8BW93; -.
DR   SMR; Q8BW93; -.
DR   IntAct; Q8BW93; 1.
DR   STRING; 10090.ENSMUSP00000159074; -.
DR   GuidetoPHARMACOLOGY; 33; -.
DR   GlyCosmos; Q8BW93; 1 site, No reported glycans.
DR   GlyGen; Q8BW93; 1 site.
DR   PhosphoSitePlus; Q8BW93; -.
DR   SwissPalm; Q8BW93; -.
DR   MaxQB; Q8BW93; -.
DR   PaxDb; 10090-ENSMUSP00000133056; -.
DR   Antibodypedia; 2937; 456 antibodies from 37 providers.
DR   DNASU; 319430; -.
DR   Ensembl; ENSMUST00000171425.4; ENSMUSP00000133056.3; ENSMUSG00000074361.12.
DR   Ensembl; ENSMUST00000238927.2; ENSMUSP00000158951.2; ENSMUSG00000074361.12.
DR   GeneID; 319430; -.
DR   KEGG; mmu:319430; -.
DR   AGR; MGI:2442013; -.
DR   CTD; 27202; -.
DR   MGI; MGI:2442013; C5ar2.
DR   VEuPathDB; HostDB:ENSMUSG00000074361; -.
DR   eggNOG; ENOG502R35Z; Eukaryota.
DR   GeneTree; ENSGT01100000263564; -.
DR   InParanoid; Q8BW93; -.
DR   OMA; HRWPLGM; -.
DR   OrthoDB; 4264357at2759; -.
DR   PhylomeDB; Q8BW93; -.
DR   Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR   Reactome; R-MMU-977606; Regulation of Complement cascade.
DR   BioGRID-ORCS; 319430; 1 hit in 76 CRISPR screens.
DR   PRO; PR:Q8BW93; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q8BW93; Protein.
DR   Bgee; ENSMUSG00000074361; Expressed in granulocyte and 26 other cell types or tissues.
DR   ExpressionAtlas; Q8BW93; baseline and differential.
DR   GO; GO:0045177; C:apical part of cell; ISS:UniProtKB.
DR   GO; GO:0009925; C:basal plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0004878; F:complement component C5a receptor activity; IBA:GO_Central.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR   GO; GO:0006935; P:chemotaxis; IEA:InterPro.
DR   GO; GO:0002430; P:complement receptor mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR   GO; GO:0090024; P:negative regulation of neutrophil chemotaxis; ISS:UniProtKB.
DR   GO; GO:0032720; P:negative regulation of tumor necrosis factor production; ISS:UniProtKB.
DR   GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR   GO; GO:0032677; P:regulation of interleukin-8 production; ISS:UniProtKB.
DR   CDD; cd15114; 7tmA_C5aR; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR002234; Anphylx_rcpt_C3a/C5a1-2.
DR   InterPro; IPR000826; Formyl_rcpt-rel.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24225:SF1; C5A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR 2; 1.
DR   PANTHER; PTHR24225; CHEMOTACTIC RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00426; C5ANPHYLTXNR.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..344
FT                   /note="C5a anaphylatoxin chemotactic receptor 2"
FT                   /id="PRO_0000303083"
FT   TOPO_DOM        1..44
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..67
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..78
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..101
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        102..120
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..143
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..155
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..178
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        179..208
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..231
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        232..243
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..266
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        267..280
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..300
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        301..344
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         325
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q695P6"
FT   CARBOHYD        3
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        113..192
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   CONFLICT        6
FT                   /note="T -> N (in Ref. 1; BAE35590)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   344 AA;  38199 MW;  508FFD23F01B31C8 CRC64;
     MMNHTTSEYY DYEYDHEHYS DLPDVPVDCP AGTCFTSDVY LIVLLVLYAA VFLVGVPGNT
     LVAWVTWKES RHRLGASWFL HLTMADLLCC VSLPFLAVPI AQKGHWPYGA AGCWLLSSIT
     ILSMYASVLL LTGLSGDLFL LAFRPSWKGA DHRTFGVRVV QASSWMLGLL LTVPSAVYRR
     LLQEHYPPRL VCGIDYGGSV SAEVAITTVR FLFGFLGPLV FMAGCHGILQ RQMARRHWPL
     GTAVVVGFFI CWTPYHVLRV IIAAAPPHSL LLARVLEAEP LFNGLALAHS ALNPIMFLYF
     GRKQLCKSLQ AACHWALRDP QDEESAVTKV SISTSHEMVS EMPV
//