C5a anaphylatoxin chemotactic receptor C5L2

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Q8BW93 (C5ARL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
C5a anaphylatoxin chemotactic receptor C5L2

Alternative name(s):
G-protein coupled receptor 77

Gene names

Name:	Gpr77
Synonyms:	C5l2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways By similarity.
Subunit structure	Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highly expressed in liver and spleen. Lower levels in intestine, brain and kidney. Also expressed in adipose tissues with highest levels in gonadal and ingual fat depots. Lower levels in brown tissue. Ref.3
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence BAE35590.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: InterPro negative regulation of interleukin-6 secretion Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neutrophil chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of tumor necrosis factor production Inferred from sequence or structural similarity. Source: UniProtKB regulation of interleukin-8 secretion Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	apical part of cell Inferred from sequence or structural similarity. Source: UniProtKB basal plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	C5a anaphylatoxin receptor activity Inferred from electronic annotation. Source: InterPro N-formyl peptide receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 344

344

C5a anaphylatoxin chemotactic receptor C5L2

PRO_0000303083

Regions

Topological domain

1 – 44

Extracellular Potential

Transmembrane

45 – 67

Helical; Name=1; Potential

Topological domain

68 – 78

Cytoplasmic Potential

Transmembrane

79 – 101

Helical; Name=2; Potential

Topological domain

102 – 120

Extracellular Potential

Transmembrane

121 – 143

Helical; Name=3; Potential

Topological domain

144 – 155

Cytoplasmic Potential

Transmembrane

156 – 178

Helical; Name=4; Potential

Topological domain

179 – 208

Extracellular Potential

Transmembrane

209 – 231

Helical; Name=5; Potential

Topological domain

232 – 243

Cytoplasmic Potential

Transmembrane

244 – 266

Helical; Name=6; Potential

Topological domain

267 – 280

Extracellular Potential

Transmembrane

281 – 300

Helical; Name=7; Potential

Topological domain

301 – 344

Cytoplasmic Potential

Amino acid modifications

Modified residue

333

Phosphoserine Ref.4

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

113 ↔ 192

By similarity

Experimental info

Sequence conflict

T → N in BAE35590. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8BW93 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 508FFD23F01B31C8
Show »

FASTA

344

38,199

        10         20         30         40         50         60 
MMNHTTSEYY DYEYDHEHYS DLPDVPVDCP AGTCFTSDVY LIVLLVLYAA VFLVGVPGNT 

        70         80         90        100        110        120 
LVAWVTWKES RHRLGASWFL HLTMADLLCC VSLPFLAVPI AQKGHWPYGA AGCWLLSSIT 

       130        140        150        160        170        180 
ILSMYASVLL LTGLSGDLFL LAFRPSWKGA DHRTFGVRVV QASSWMLGLL LTVPSAVYRR 

       190        200        210        220        230        240 
LLQEHYPPRL VCGIDYGGSV SAEVAITTVR FLFGFLGPLV FMAGCHGILQ RQMARRHWPL 

       250        260        270        280        290        300 
GTAVVVGFFI CWTPYHVLRV IIAAAPPHSL LLARVLEAEP LFNGLALAHS ALNPIMFLYF 

       310        320        330        340 
GRKQLCKSLQ AACHWALRDP QDEESAVTKV SISTSHEMVS EMPV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"C5L2 is a functional receptor for acylation-stimulating protein." Kalant D., MacLaren R., Cui W., Samanta R., Monk P.N., Laporte S.A., Cianflone K. J. Biol. Chem. 280:23936-23944(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK053187 mRNA. Translation: BAC35303.1. AK160050 mRNA. Translation: BAE35590.1. Different initiation. BC137941 mRNA. Translation: AAI37942.1. BC145834 mRNA. Translation: AAI45835.1.
IPI	IPI00226183.
RefSeq	NP_001139477.1. NM_001146005.1. NP_795886.2. NM_176912.4.
UniGene	Mm.239570.
3D structure databases
ProteinModelPortal	Q8BW93.
SMR	Q8BW93. Positions 42-338.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q8BW93.
Proteomic databases
PRIDE	Q8BW93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	319430.
KEGG	mmu:319430.
UCSC	uc009fhk.2. mouse.
Organism-specific databases
CTD	27202.
MGI	MGI:2442013. Gpr77.
Phylogenomic databases
eggNOG	NOG151055.
HOGENOM	HOG000234122.
HOVERGEN	HBG107547.
InParanoid	B2RQI1.
KO	K04171.
OrthoDB	EOG4VQ9Q1.
Gene expression databases
ArrayExpress	Q8BW93.
Bgee	Q8BW93.
CleanEx	MM_GPR77.
Genevestigator	Q8BW93.
Family and domain databases
InterPro	IPR001274. C5A_anaphtx_rcpt. IPR000826. Formyl_pep_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24225. PTHR24225. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00426. C5ANPHYLTXNR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	394718.
SOURCE	Search...

Entry information

Entry name

C5ARL_MOUSE

Accession

Primary (citable) accession number: Q8BW93
Secondary accession number(s): B2RQI1, Q3TVM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents