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Protein

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2

Gene

Pomgnt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that tranfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein (By similarity). Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:24256719).By similarity1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-O-alpha-D-mannosylprotein.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • neuron migration Source: UniProtKB
  • protein O-linked glycosylation Source: UniProtKB
  • protein O-linked mannosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (EC:2.4.1.-By similarity)
Short name:
POMGnT2
Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase-like
Glycosyltransferase-like domain-containing protein 2
Gene namesi
Name:Pomgnt2
Synonyms:Ago61, Eogtl, Gtdc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2143424. Pomgnt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 580LumenalSequence analysisAdd BLAST555

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Newborn are slightly smaller and die within the first day of birth due to abnormal basal lamina formation and neuronal migration defects. Defects are due to a lack of laminin-binding glycans.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002490151 – 605Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2Add BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BW41.
PeptideAtlasiQ8BW41.
PRIDEiQ8BW41.

PTM databases

PhosphoSitePlusiQ8BW41.

Expressioni

Tissue specificityi

Mainly expressed in the central nervous system.1 Publication

Developmental stagei

Expressed during brain development. At 14.5 dpc, expressed throughout the cortical plate and the developing brain, as well as throughout the eye, including the lens and cornea. Cortical expression is already detected at 12.5 dpc, peaks between 14.5 and 16.5 dpc and is reduced at birth.1 Publication

Gene expression databases

BgeeiENSMUSG00000066235.
CleanExiMM_C85492.
GenevisibleiQ8BW41. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095868.

Structurei

3D structure databases

ProteinModelPortaliQ8BW41.
SMRiQ8BW41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini488 – 584Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the glycosyltransferase 61 family.Curated
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4698. Eukaryota.
ENOG410ZHQA. LUCA.
GeneTreeiENSGT00770000120599.
HOGENOMiHOG000006878.
HOVERGENiHBG054852.
InParanoidiQ8BW41.
KOiK18207.
OMAiYAVNPDH.
OrthoDBiEOG091G03LB.
PhylomeDBiQ8BW41.
TreeFamiTF332712.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR007657. Glycosyltransferase_AER61.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BW41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLSAVFNAL LVSVLAAVLW KHVRLREHAA TLEEELALGQ QSLDPVLGLK
60 70 80 90 100
IDYPKALQIL MEGGTHMVCT GRTHTDRICR FKWLCYSNEA EEFIFFHGNS
110 120 130 140 150
SVMLPNLGSR RFQPALLDLS TVEDHNAQYF NFVELPAAAL RFMPKPVFVP
160 170 180 190 200
DVALIANRFN PDNLMHVFHD DLLPLFYTLR QFPGLAQEAR LFFMEGWGEG
210 220 230 240 250
AHFDLYKLLS PKQPLLRAQL KTLGRLLCFS HAFVGLSKVT TWYQYGFVQP
260 270 280 290 300
QGPKANILVS GNEIRQFTRF MTERLNVSHA GAPLGEEYIL VFSRTQNRLI
310 320 330 340 350
LNEAELLLEL AQEFQMKTVT VSLEDHTFAD VVRLVSNASM LVSMHGAQLV
360 370 380 390 400
TALFLPRGAT VVELFPYAVN PDHYTPYKTL ATLPGMDLQY VAWRNMIREN
410 420 430 440 450
TVTHPERPWD QGGITHLDRA EQARILQSRE VPRHLCCRNP EWLFRIYQDT
460 470 480 490 500
RVDIPSLMQS IRRVVKGRPG PRRQRWAISL YPGKVREARC QASVQGATEA
510 520 530 540 550
RLSVSWQIPW NLKYLKVREV KYEVWLQEQG ENTYVPYMLT LQNHTFTENI
560 570 580 590 600
KPFTTYLVWV RCIFNRSLLG PFCRCAGVQH VASRPWPGLW RAPLAGSAFP

GPQFC
Length:605
Mass (Da):69,378
Last modified:March 1, 2003 - v1
Checksum:i2A70A214653D9E12
GO
Isoform 2 (identifier: Q8BW41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     573-580: CRCAGVQH → ADVLVCST
     581-605: Missing.

Show »
Length:580
Mass (Da):66,661
Checksum:iA687B5A5BFB7F01D
GO

Sequence cautioni

The sequence BAC31348 differs from that shown. Reason: Erroneous termination at position 525. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567S → T in AAH30931 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020354573 – 580CRCAGVQH → ADVLVCST in isoform 2. 2 Publications8
Alternative sequenceiVSP_020355581 – 605Missing in isoform 2. 2 PublicationsAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042747 mRNA. Translation: BAC31348.1. Sequence problems.
AK054403 mRNA. Translation: BAC35765.1.
AK133802 mRNA. Translation: BAE21851.1.
BC025056 mRNA. Translation: AAH25056.1.
BC030931 mRNA. Translation: AAH30931.1.
CCDSiCCDS23643.1. [Q8BW41-2]
RefSeqiNP_001276487.1. NM_001289558.1. [Q8BW41-2]
NP_001276488.1. NM_001289559.1. [Q8BW41-2]
NP_001276489.1. NM_001289560.1. [Q8BW41-2]
NP_705768.4. NM_153540.4. [Q8BW41-2]
XP_011241257.1. XM_011242955.1. [Q8BW41-2]
UniGeneiMm.402151.

Genome annotation databases

EnsembliENSMUST00000084743; ENSMUSP00000095868; ENSMUSG00000066235. [Q8BW41-2]
GeneIDi215494.
KEGGimmu:215494.
UCSCiuc009sek.2. mouse. [Q8BW41-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042747 mRNA. Translation: BAC31348.1. Sequence problems.
AK054403 mRNA. Translation: BAC35765.1.
AK133802 mRNA. Translation: BAE21851.1.
BC025056 mRNA. Translation: AAH25056.1.
BC030931 mRNA. Translation: AAH30931.1.
CCDSiCCDS23643.1. [Q8BW41-2]
RefSeqiNP_001276487.1. NM_001289558.1. [Q8BW41-2]
NP_001276488.1. NM_001289559.1. [Q8BW41-2]
NP_001276489.1. NM_001289560.1. [Q8BW41-2]
NP_705768.4. NM_153540.4. [Q8BW41-2]
XP_011241257.1. XM_011242955.1. [Q8BW41-2]
UniGeneiMm.402151.

3D structure databases

ProteinModelPortaliQ8BW41.
SMRiQ8BW41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095868.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

PTM databases

PhosphoSitePlusiQ8BW41.

Proteomic databases

PaxDbiQ8BW41.
PeptideAtlasiQ8BW41.
PRIDEiQ8BW41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084743; ENSMUSP00000095868; ENSMUSG00000066235. [Q8BW41-2]
GeneIDi215494.
KEGGimmu:215494.
UCSCiuc009sek.2. mouse. [Q8BW41-2]

Organism-specific databases

CTDi84892.
MGIiMGI:2143424. Pomgnt2.

Phylogenomic databases

eggNOGiKOG4698. Eukaryota.
ENOG410ZHQA. LUCA.
GeneTreeiENSGT00770000120599.
HOGENOMiHOG000006878.
HOVERGENiHBG054852.
InParanoidiQ8BW41.
KOiK18207.
OMAiYAVNPDH.
OrthoDBiEOG091G03LB.
PhylomeDBiQ8BW41.
TreeFamiTF332712.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-5173105. O-linked glycosylation.

Miscellaneous databases

PROiQ8BW41.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066235.
CleanExiMM_C85492.
GenevisibleiQ8BW41. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR007657. Glycosyltransferase_AER61.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMGT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BW41
Secondary accession number(s): Q58F17, Q8BXZ9, Q8K0M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.