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Protein

Calcium-responsive transactivator

Gene

Ss18l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.2 Publications

GO - Molecular functioni

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • dendrite development Source: MGI
  • positive regulation of dendrite morphogenesis Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-responsive transactivator
Alternative name(s):
SS18-like protein 1
Gene namesi
Name:Ss18l1
Synonyms:Crest, Kiaa0693
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444061. Ss18l1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: MGI
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • condensed nuclear chromosome, centromeric region Source: MGI
  • kinetochore Source: MGI
  • nBAF complex Source: MGI
  • nuclear body Source: Ensembl
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are smaller than littermates and show coordination defects. There is increased mortality in beginning at about P14, and about 80% die by P28. Less than 20% survive to adulthood, and they are infertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913461 – 402Calcium-responsive transactivatorAdd BLAST402

Proteomic databases

MaxQBiQ8BW22.
PaxDbiQ8BW22.
PeptideAtlasiQ8BW22.
PRIDEiQ8BW22.

PTM databases

iPTMnetiQ8BW22.
PhosphoSitePlusiQ8BW22.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039086.
GenevisibleiQ8BW22. MM.

Interactioni

Subunit structurei

Homodimer. Dimerization may be necessary for its function in neuronal dendritic development. Interacts (via C-terminus) with CREBBP (via N-terminus), EP300 and SMARCA4/BRG1. Interacts with the nBAF complex. Association with CREBBP facilitates transcription while the association with SMARCA4/BRG1 suppresses CREST-mediated transcription in resting neurons (By similarity).By similarity

Protein-protein interaction databases

BioGridi234646. 2 interactors.
IntActiQ8BW22. 1 interactor.
MINTiMINT-4112955.
STRINGi10090.ENSMUSP00000041288.

Structurei

3D structure databases

ProteinModelPortaliQ8BW22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 148N-terminal auto-inhibitory domain; necessary for interaction with SMARCA4/BRG1By similarityAdd BLAST148
Regioni149 – 238Methionine-rich intra-molecular domainBy similarityAdd BLAST90
Regioni252 – 323MFD domainBy similarityAdd BLAST72
Regioni340 – 402Necessary for nuclear localizationBy similarityAdd BLAST63
Regioni393 – 402Necessary for interaction with CREBBP and for the recruitment of CREBBP to the nuclear bodiesBy similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 53SH2-bindingSequence analysis4
Motifi359 – 362SH2-bindingSequence analysis4
Motifi377 – 385SH3-bindingSequence analysis9
Motifi397 – 400SH2-bindingSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi197 – 402Gln-richAdd BLAST206

Domaini

The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization.By similarity

Sequence similaritiesi

Belongs to the SS18 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3227. Eukaryota.
ENOG41128HA. LUCA.
GeneTreeiENSGT00500000044808.
HOGENOMiHOG000048717.
HOVERGENiHBG003892.
InParanoidiQ8BW22.
OMAiQMLDENH.
OrthoDBiEOG091G0O10.
PhylomeDBiQ8BW22.
TreeFamiTF330999.

Family and domain databases

InterProiIPR007726. SS18_fam.
[Graphical view]
PANTHERiPTHR23107. PTHR23107. 1 hit.
PfamiPF05030. SSXT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BW22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAFASARP RGKGEVTQQT IQKMLDENHH LIQCILDYQS KGKTAECTQY
60 70 80 90 100
QQILHRNLVY LATIADSNQN MQSLLPAPPT QNMNLGPGAL SQSGSSQGLH
110 120 130 140 150
PQGSLSDTVS TGLPPASLMQ GQIGNGPNHV SMQQTAQSTL PTTSMSLSGS
160 170 180 190 200
GHGTGPGYSH SGPTSQSVPM QGQGAISNYV SRTNINMQSN PVSMMHQQAA
210 220 230 240 250
TSHYNSAQGG SQHYQGQAPI AMMGQGGQGG SMMGQRPMAP YRPSQQGSSQ
260 270 280 290 300
QYLGQEEYYS EQYSHSQGSA EPMSQQYYPD GHGDYAYQQS SYTEQSYDRS
310 320 330 340 350
FEDPTQHYYE GGNSQYSQQQ AGYQQGTAQQ QTYSQQQYPN QQSYPGQQQG
360 370 380 390 400
YGPAQGAPSQ YSSYQQGQGQ QYGSYRTSQT GPSAQQQRPY GYEQGQYGNY

QQ
Length:402
Mass (Da):43,729
Last modified:March 1, 2003 - v1
Checksum:i9C309B9E7446CCAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130V → L in BAC30089 (PubMed:16141072).Curated1
Sequence conflicti159S → I in AAH54730 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173004 mRNA. Translation: BAD32282.1.
AK038669 mRNA. Translation: BAC30089.1.
AK054560 mRNA. Translation: BAC35824.1.
AK133944 mRNA. Translation: BAE21942.1.
AL663067 Genomic DNA. Translation: CAM18353.1.
BC053087 mRNA. Translation: AAH53087.1.
BC054730 mRNA. Translation: AAH54730.1.
CCDSiCCDS17167.1.
RefSeqiNP_848865.4. NM_178750.5.
UniGeneiMm.63068.

Genome annotation databases

EnsembliENSMUST00000041126; ENSMUSP00000041288; ENSMUSG00000039086.
GeneIDi269397.
KEGGimmu:269397.
UCSCiuc008oic.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173004 mRNA. Translation: BAD32282.1.
AK038669 mRNA. Translation: BAC30089.1.
AK054560 mRNA. Translation: BAC35824.1.
AK133944 mRNA. Translation: BAE21942.1.
AL663067 Genomic DNA. Translation: CAM18353.1.
BC053087 mRNA. Translation: AAH53087.1.
BC054730 mRNA. Translation: AAH54730.1.
CCDSiCCDS17167.1.
RefSeqiNP_848865.4. NM_178750.5.
UniGeneiMm.63068.

3D structure databases

ProteinModelPortaliQ8BW22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234646. 2 interactors.
IntActiQ8BW22. 1 interactor.
MINTiMINT-4112955.
STRINGi10090.ENSMUSP00000041288.

PTM databases

iPTMnetiQ8BW22.
PhosphoSitePlusiQ8BW22.

Proteomic databases

MaxQBiQ8BW22.
PaxDbiQ8BW22.
PeptideAtlasiQ8BW22.
PRIDEiQ8BW22.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041126; ENSMUSP00000041288; ENSMUSG00000039086.
GeneIDi269397.
KEGGimmu:269397.
UCSCiuc008oic.2. mouse.

Organism-specific databases

CTDi26039.
MGIiMGI:2444061. Ss18l1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3227. Eukaryota.
ENOG41128HA. LUCA.
GeneTreeiENSGT00500000044808.
HOGENOMiHOG000048717.
HOVERGENiHBG003892.
InParanoidiQ8BW22.
OMAiQMLDENH.
OrthoDBiEOG091G0O10.
PhylomeDBiQ8BW22.
TreeFamiTF330999.

Miscellaneous databases

PROiQ8BW22.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039086.
GenevisibleiQ8BW22. MM.

Family and domain databases

InterProiIPR007726. SS18_fam.
[Graphical view]
PANTHERiPTHR23107. PTHR23107. 1 hit.
PfamiPF05030. SSXT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCREST_MOUSE
AccessioniPrimary (citable) accession number: Q8BW22
Secondary accession number(s): Q6A014, Q7TQF3, Q8BYQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.