Reviewed, UniProtKB/Swiss-Prot Q8BW11 (TRYX3_MOUSE)

Names and origin

Protein names

Recommended name:
    Putative trypsin-X3
    EC=3.4.21.4

Gene names

Name:

Tryx3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Caution	Thr-195 is present instead of the conserved Ser which is expected to be an active site residue. It is therefore unsure if this protein has kept its catalytic activity.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 241	224	Putative trypsin-X3		PRO_0000317764
Regions
	Domain	18 – 239	222	Peptidase S1
Sites
	Active site	56	1	Charge relay system By similarity
	Active site	101	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 57		By similarity
	Disulfide bond	133 ↔ 201		By similarity
	Disulfide bond	165 ↔ 180		By similarity
	Disulfide bond	191 ↔ 215		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8BW11-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8FC9D0D994A4AE7F	FASTA	241	26,865
	10 20 30 40 50 60 MKLAFLCILS TLLRTFAYNP DHIAGTTPPY LVYLKSDYLP CTGVLIHPLW VITAAHCNLP 70 80 90 100 110 120 NLQVILGITN PADPMERDVE VSDYEKIFHH PNFLVSSISH DLLLIKLKRR IKHSNYAKAV 130 140 150 160 170 180 KLPQHIVSVN AMCSVSTWAY NLCDVTKDPD SLQTVNVTVI SKAECRNAYK AFDITENMIC 190 200 210 220 230 240 VGIVPGRRLP CKEVTAAPAV CNGVLYGILS YADGCVLRAD VGIYASIFHY LPWIEDTMKN N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential transcriptional regulation of individual TCR V beta segments before gene rearrangement." Chen F., Rowen L., Hood L., Rothenberg E.V. J. Immunol. 166:1771-1780(2001) [PubMed: 11160223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000663 Genomic DNA. Translation: AAB69042.1. AK075754 mRNA. Translation: BAC35932.1. BC048599 mRNA. Translation: AAH48599.1.
IPI	IPI00225978.
RefSeq	NP_778185.1.
UniGene	Mm.56997
3D structure databases
HSSP	HSSP built from PDB template 2HNT based on UniProtKB P00734.
ModBase	Search...
Protein family/group databases
MEROPS	S01.984.
Genome annotation databases
Ensembl	ENSMUSG00000051936. Mus musculus. [Contig view]
GeneID	232717.
KEGG	mmu:232717.
Organism-specific databases
MGI	MGI:3608323. BC048599.
Phylogenomic databases
HOGENOM	Q8BW11.
HOVERGEN	Q8BW11.
OMA	Q8BW11. VSTWAYN.
Enzyme and pathway databases
BRENDA	3.4.21.4. 244.
Gene expression databases
ArrayExpress	Q8BW11.
Bgee	Q8BW11.
CleanEx	MM_BC048599.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	381207.
SOURCE	Search...

Entry information

Entry name

TRYX3_MOUSE

Accession

Primary (citable) accession number: Q8BW11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)