Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8BVZ5 (IL33_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-33

Short name=IL-33
Gene names
Name:Il33
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 By similarity. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. Ref.1 Ref.4

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. Ref.1 Ref.4

Subunit structure

Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP By similarity.

Subcellular location

Nucleus. Chromosome. Cytoplasmic vesiclesecretory vesicle By similarity. Secreted. Note: Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent By similarity. Ref.4

Domain

The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association By similarity.

Post-translational modification

Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Proteolysis is not strictly required for biological activity By similarity.

Miscellaneous

Intraperitoneal injections of IL-33 induce the expression of IL-4, IL-5, and IL-13 and lead to severe pathological changes in mucosal organs.

Sequence similarities

Belongs to the IL-1 family. Highly divergent.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentChromosome
Cytoplasmic vesicle
Nucleus
Secreted
   Molecular functionCytokine
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processextrinsic apoptotic signaling pathway

Inferred from genetic interaction PubMed 22660580. Source: MGI

negative regulation of T-helper 1 type immune response

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

negative regulation of immunoglobulin secretion

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

negative regulation of interferon-gamma production

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

negative regulation of leukocyte migration

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of chemokine secretion

Inferred from direct assay PubMed 19841166. Source: BHF-UCL

positive regulation of gene expression

Inferred from direct assay PubMed 19666510. Source: MGI

positive regulation of immunoglobulin secretion

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of inflammatory response

Inferred from direct assay PubMed 19841166. Source: BHF-UCL

positive regulation of interleukin-13 production

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of interleukin-4 production

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of interleukin-5 production

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of interleukin-6 production

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

positive regulation of macrophage activation

Inferred from direct assay PubMed 19841166. Source: BHF-UCL

positive regulation of proteasomal ubiquitin-dependent protein catabolic process

Inferred from direct assay PubMed 22660580. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19841166. Source: BHF-UCL

positive regulation of type 2 immune response

Inferred from genetic interaction PubMed 18268038. Source: BHF-UCL

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchromosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from sequence orthology PubMed 12819012. Source: MGI

transport vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 19841166. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 108108 By similarity
PRO_0000248846
Chain109 – 266158Interleukin-33
PRO_0000096791

Regions

Region1 – 6565Homeodomain-like HTH domain By similarity
Region66 – 10843Interaction with RELA

Experimental info

Sequence conflict24 – 252AL → RS in AAX86999. Ref.1
Sequence conflict1791L → V in AAH03847. Ref.3
Sequence conflict1851P → S in AAX86999. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BVZ5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E03C2C297EB43E23

FASTA26629,991
        10         20         30         40         50         60 
MRPRMKYSNS KISPAKFSST AGEALVPPCK IRRSQQKTKE FCHVYCMRLR SGLTIRKETS 

        70         80         90        100        110        120 
YFRKEPTKRY SLKSGTKHEE NFSAYPRDSR KRSLLGSIQA FAASVDTLSI QGTSLLTQSP 

       130        140        150        160        170        180 
ASLSTYNDQS VSFVLENGCY VINVDDSGKD QEQDQVLLRY YESPCPASQS GDGVDGKKLM 

       190        200        210        220        230        240 
VNMSPIKDTD IWLHANDKDY SVELQRGDVS PPEQAFFVLH KKSSDFVSFE CKNLPGTYIG 

       250        260 
VKDNQLALVE EKDESCNNIM FKLSKI 

« Hide

References

« Hide 'large scale' references
[1]"IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST 2 and induces T helper type 2-associated cytokines."
Schmitz J., Owyang A., Oldham E., Song Y., Murphy E., McClanahan T.K., Zurawski G., Moshrefi M., Qin J., Li X., Gorman D.M., Bazan J.F., Kastelein R.A.
Immunity 23:479-490(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpora quadrigemina and Gastric mucosa.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[4]"The dual function cytokine IL-33 interacts with the transcription factor NF-kappaB to dampen NF-kappaB-stimulated gene transcription."
Ali S., Mohs A., Thomas M., Klare J., Ross R., Schmitz M.L., Martin M.U.
J. Immunol. 187:1609-1616(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NF-KAPPAB/RELA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY905582 mRNA. Translation: AAX86999.1.
AK075849 mRNA. Translation: BAC36003.1.
AK163464 mRNA. Translation: BAE37352.1.
BC003847 mRNA. Translation: AAH03847.1.
RefSeqNP_001158196.1. NM_001164724.1.
NP_598536.2. NM_133775.2.
XP_006527526.1. XM_006527463.1.
UniGeneMm.182359.

3D structure databases

ProteinModelPortalQ8BVZ5.
SMRQ8BVZ5. Positions 109-265.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid218533. 1 interaction.
IntActQ8BVZ5. 1 interaction.

PTM databases

PhosphoSiteQ8BVZ5.

Proteomic databases

PRIDEQ8BVZ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025724; ENSMUSP00000025724; ENSMUSG00000024810.
ENSMUST00000120388; ENSMUSP00000113829; ENSMUSG00000024810.
GeneID77125.
KEGGmmu:77125.
UCSCuc008hec.2. mouse.

Organism-specific databases

CTD90865.
MGIMGI:1924375. Il33.

Phylogenomic databases

eggNOGNOG41297.
GeneTreeENSGT00390000005185.
HOGENOMHOG000070215.
HOVERGENHBG081791.
InParanoidQ8BVZ5.
KOK12967.
OMADPGVFIG.
OrthoDBEOG7TQV1R.
PhylomeDBQ8BVZ5.
TreeFamTF338120.

Gene expression databases

ArrayExpressQ8BVZ5.
BgeeQ8BVZ5.
CleanExMM_IL33.
GenevestigatorQ8BVZ5.

Family and domain databases

InterProIPR026145. IL-33.
[Graphical view]
PANTHERPTHR21114. PTHR21114. 1 hit.
ProtoNetSearch...

Other

NextBio346526.
PROQ8BVZ5.
SOURCESearch...

Entry information

Entry nameIL33_MOUSE
AccessionPrimary (citable) accession number: Q8BVZ5
Secondary accession number(s): Q2YEJ4, Q3TQN0, Q99L46
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot