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Protein

Protein phosphatase methylesterase 1

Gene

Ppme1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).By similarity

Catalytic activityi

[Phosphatase 2A protein]-leucine methyl ester + H2O = [phosphatase 2A protein]-leucine + methanol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156By similarity1
Active sitei181By similarity1
Active sitei349By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-69273. Cyclin A/B1/B2 associated events during G2/M transition.

Protein family/group databases

ESTHERimouse-PPME1. PPase_methylesterase_euk.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase methylesterase 1 (EC:3.1.1.89)
Short name:
PME-1
Gene namesi
Name:Ppme1
Synonyms:Pme1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919840. Ppme1.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2189138.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000903911 – 386Protein phosphatase methylesterase 1Add BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1
Modified residuei16Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei16Omega-N-methylarginine; alternateBy similarity1

Post-translational modificationi

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ8BVQ5.
MaxQBiQ8BVQ5.
PaxDbiQ8BVQ5.
PeptideAtlasiQ8BVQ5.
PRIDEiQ8BVQ5.

PTM databases

iPTMnetiQ8BVQ5.
PhosphoSitePlusiQ8BVQ5.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in testis and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000030718.
CleanExiMM_PPME1.
ExpressionAtlasiQ8BVQ5. baseline and differential.
GenevisibleiQ8BVQ5. MM.

Interactioni

Subunit structurei

Binds PPP2CA and PPP2CB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46211N.
IntActiQ8BVQ5. 5 interactors.
MINTiMINT-4108015.
STRINGi10090.ENSMUSP00000032963.

Chemistry databases

BindingDBiQ8BVQ5.

Structurei

3D structure databases

ProteinModelPortaliQ8BVQ5.
SMRiQ8BVQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi256 – 263Poly-Glu8

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG2564. Eukaryota.
COG0596. LUCA.
GeneTreeiENSGT00390000004396.
HOVERGENiHBG053622.
InParanoidiQ8BVQ5.
KOiK13617.
OMAiRIQCRIV.
OrthoDBiEOG091G0D0P.
PhylomeDBiQ8BVQ5.
TreeFamiTF314697.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR016812. PPase_methylesterase_euk.
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiView protein in Pfam
PF12697. Abhydrolase_6. 1 hit.
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiView protein in PROSITE
PS00120. LIPASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BVQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FTPVPWSQYF
60 70 80 90 100
ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII
110 120 130 140 150
SRVQCRIVAL DLRGHGETKV KNSEDLSAET MAKDVGNVVE AMYGDLPPPV
160 170 180 190 200
MLIGHSMGGA IAVHTAAANL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG
210 220 230 240 250
RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI
260 270 280 290 300
VEGIIEEEEE DEEGSESVNK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG
310 320 330 340 350
WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA
360 370 380
VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
Length:386
Mass (Da):42,256
Last modified:January 23, 2007 - v5
Checksum:i2BD07B588299DB6D
GO

Sequence cautioni

Q8BVQ5: The sequence BAE32896 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17P → T in BAE27027 (PubMed:16141072).Curated1
Sequence conflicti86G → D in AAH29064 (PubMed:15489334).Curated1
Sequence conflicti370H → R in BAE27027 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012256 mRNA. Translation: BAB28122.1.
AK146051 mRNA. Translation: BAE26861.1.
AK146268 mRNA. Translation: BAE27027.1.
AK154876 mRNA. Translation: BAE32896.1. Different initiation.
BC014867 mRNA. Translation: AAH14867.1.
BC029064 mRNA. Translation: AAH29064.1.
CCDSiCCDS40036.1.
RefSeqiNP_082568.1. NM_028292.2.
UniGeneiMm.177502.
Mm.490280.

Genome annotation databases

EnsembliENSMUST00000032963; ENSMUSP00000032963; ENSMUSG00000030718.
GeneIDi72590.
KEGGimmu:72590.
UCSCiuc009imu.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPPME1_MOUSE
AccessioniPrimary (citable) accession number: Q8BVQ5
Secondary accession number(s): Q3U392
, Q3UJX8, Q3UKE0, Q8K311, Q91YR8, Q9CSP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 126 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families