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Protein

Cation channel sperm-associated protein 4

Gene

Catsper4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.2 Publications

Enzyme regulationi

In contrast to the human ortholog, not activated by progesterone.1 Publication

GO - Molecular functioni

  • calcium activated cation channel activity Source: InterPro
  • voltage-gated calcium channel activity Source: MGI
  • voltage-gated sodium channel activity Source: GO_Central

GO - Biological processi

  • membrane depolarization during action potential Source: GO_Central
  • multicellular organism development Source: UniProtKB-KW
  • neuronal action potential Source: GO_Central
  • sodium ion transport Source: MGI
  • sperm capacitation Source: MGI
  • sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Developmental protein, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Differentiation, Ion transport, Spermatogenesis, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation channel sperm-associated protein 4
Short name:
CatSper4
Gene namesi
Name:Catsper4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3043288. Catsper4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666CytoplasmicSequence analysisAdd
BLAST
Transmembranei67 – 8721Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini88 – 10619ExtracellularSequence analysisAdd
BLAST
Transmembranei107 – 12721Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini128 – 1369CytoplasmicSequence analysis
Transmembranei137 – 15721Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini158 – 1581ExtracellularSequence analysis
Transmembranei159 – 17315Helical; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini174 – 19522CytoplasmicSequence analysisAdd
BLAST
Transmembranei196 – 21621Helical; Name=Segment S5Add
BLAST
Topological domaini217 – 2226ExtracellularSequence analysis
Intramembranei223 – 24321Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini244 – 26017ExtracellularSequence analysisAdd
BLAST
Transmembranei261 – 28121Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini282 – 442161CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • CatSper complex Source: UniProtKB
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Flagellum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males are sterile. Male sterility is due to defects in sperm motility unability to fertilize intact eggs.2 Publications

Chemistry

GuidetoPHARMACOLOGYi391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Cation channel sperm-associated protein 4PRO_0000295682Add
BLAST

Proteomic databases

PaxDbiQ8BVN3.
PRIDEiQ8BVN3.

PTM databases

PhosphoSiteiQ8BVN3.

Expressioni

Tissue specificityi

Testis-specific.2 Publications

Developmental stagei

Detected in hte testis during postnatal development at day 15. Restricted to the late-stage germline cells that line the seminiferous tubules.1 Publication

Gene expression databases

BgeeiQ8BVN3.
CleanExiMM_CATSPER4.
ExpressionAtlasiQ8BVN3. baseline and differential.

Interactioni

Subunit structurei

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER1.Curated2 Publications

Protein-protein interaction databases

BioGridi236869. 1 interaction.
DIPiDIP-60803N.
STRINGi10090.ENSMUSP00000051694.

Structurei

3D structure databases

ProteinModelPortaliQ8BVN3.
SMRiQ8BVN3. Positions 70-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II5Y. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000119325.
HOGENOMiHOG000112053.
HOVERGENiHBG102124.
InParanoidiQ8BVN3.
KOiK16892.
OMAiWLNGFWI.
OrthoDBiEOG7R831F.
PhylomeDBiQ8BVN3.
TreeFamiTF343841.

Family and domain databases

InterProiIPR028744. CatSper4.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERiPTHR10037:SF225. PTHR10037:SF225. 2 hits.
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BVN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKHKWWQQ VENIDITHLG PKRKAYELLG RHEEQVLINR RDVMEKKDAW
60 70 80 90 100
DVQEFITQMY IKQLLRHPAF QLLLAFLLLS NAITIALRTN SYLGQKHYEL
110 120 130 140 150
FSTIDDIVLT ILICEVLLGW LNGFWIFWKD GWNILNFAIV FILFMGFFIK
160 170 180 190 200
QLDMVAITYP LRVLRLVHVC MAVEPLARII KVILQSMPDL ANVMALILFF
210 220 230 240 250
MLVFSVFGVT LFGAFVPKHF QNMGVALYTL FICITQDGWL DIYTDFQMDE
260 270 280 290 300
REYAMEVGGA IYFAVFITLG AFIGLNLFVV VVTTNLEQMM KTGEEEGHLN
310 320 330 340 350
IKFTETEEDE DWTDELPLVH CTEARKDTST VPKEPLVGGP LSNLTEKTCD
360 370 380 390 400
NFCLVLEAIQ ENLMEYKEIR EELNMIVEEV SSIRFNQEQQ NVILHKYTSK
410 420 430 440
SATFLSEPPE GANKQDLITA LVSREKVSDS NINMVNKHKF SH
Length:442
Mass (Da):51,130
Last modified:March 1, 2003 - v1
Checksum:iB4A41CAA47F0AE21
GO

Sequence cautioni

The sequence CAM21941.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077145 mRNA. Translation: BAC36641.1.
AL627314 Genomic DNA. Translation: CAM21940.1.
AL627314 Genomic DNA. Translation: CAM21941.1. Sequence problems.
BC108974 mRNA. Translation: AAI08975.1.
BC108975 mRNA. Translation: AAI08976.1.
CCDSiCCDS18766.1.
RefSeqiNP_001123502.1. NM_001130030.1.
NP_808534.1. NM_177866.4.
UniGeneiMm.79072.

Genome annotation databases

EnsembliENSMUST00000055892; ENSMUSP00000051694; ENSMUSG00000048003.
GeneIDi329954.
KEGGimmu:329954.
UCSCiuc008veh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077145 mRNA. Translation: BAC36641.1.
AL627314 Genomic DNA. Translation: CAM21940.1.
AL627314 Genomic DNA. Translation: CAM21941.1. Sequence problems.
BC108974 mRNA. Translation: AAI08975.1.
BC108975 mRNA. Translation: AAI08976.1.
CCDSiCCDS18766.1.
RefSeqiNP_001123502.1. NM_001130030.1.
NP_808534.1. NM_177866.4.
UniGeneiMm.79072.

3D structure databases

ProteinModelPortaliQ8BVN3.
SMRiQ8BVN3. Positions 70-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236869. 1 interaction.
DIPiDIP-60803N.
STRINGi10090.ENSMUSP00000051694.

Chemistry

GuidetoPHARMACOLOGYi391.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiQ8BVN3.

Proteomic databases

PaxDbiQ8BVN3.
PRIDEiQ8BVN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055892; ENSMUSP00000051694; ENSMUSG00000048003.
GeneIDi329954.
KEGGimmu:329954.
UCSCiuc008veh.2. mouse.

Organism-specific databases

CTDi378807.
MGIiMGI:3043288. Catsper4.

Phylogenomic databases

eggNOGiENOG410II5Y. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000119325.
HOGENOMiHOG000112053.
HOVERGENiHBG102124.
InParanoidiQ8BVN3.
KOiK16892.
OMAiWLNGFWI.
OrthoDBiEOG7R831F.
PhylomeDBiQ8BVN3.
TreeFamiTF343841.

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Miscellaneous databases

PROiQ8BVN3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BVN3.
CleanExiMM_CATSPER4.
ExpressionAtlasiQ8BVN3. baseline and differential.

Family and domain databases

InterProiIPR028744. CatSper4.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERiPTHR10037:SF225. PTHR10037:SF225. 2 hits.
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Catsper3 and catsper4 encode two cation channel-like proteins exclusively expressed in the testis."
    Jin J.-L., O'Doherty A.M., Wang S., Zheng H., Sanders K.M., Yan W.
    Biol. Reprod. 73:1235-1242(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility."
    Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L.
    Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Catsper3 and catsper4 are essential for sperm hyperactivated motility and male fertility in the mouse."
    Jin J.-L., Jin N., Zheng H., Ro S., Tafolla D., Sanders K.M., Yan W.
    Biol. Reprod. 77:37-44(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility."
    Qi H., Moran M.M., Navarro B., Chong J.A., Krapivinsky G., Krapivinsky L., Kirichok Y., Ramsey I.S., Quill T.A., Clapham D.E.
    Proc. Natl. Acad. Sci. U.S.A. 104:1219-1223(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH CATSPER1, DISRUPTION PHENOTYPE.
  8. "Progesterone activates the principal Ca2+ channel of human sperm."
    Lishko P.V., Botchkina I.L., Kirichok Y.
    Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ACTIVATION BY PROGESTERONE AND PGE1.
  9. "A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa."
    Chung J.J., Navarro B., Krapivinsky G., Krapivinsky L., Clapham D.E.
    Nat. Commun. 2:153-153(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CATSPER COMPLEX.
    Strain: C57BL/6J.

Entry informationi

Entry nameiCTSR4_MOUSE
AccessioniPrimary (citable) accession number: Q8BVN3
Secondary accession number(s): A2A9K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.