Q8BVK9 (SP110_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sp110 nuclear body protein

Alternative name(s):
Intracellular pathogen resistance protein 1

Gene names

Name:	Sp110
Synonyms:	Ifi75, Ipr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a transcription factor. Plays a role in the innate immunity against intracellular pathogens. Required for resistance to M.tuberculosis and L.monocytogenes. Promotes apoptosis of infected cells. Ref.1
Subcellular location	Nucleus By similarity.
Tissue specificity	Detected in lung and macrophages. Ref.1
Induction	Up-regulated after infection with M.tuberculosis. Ref.1
Involvement in disease	Defects in Sp110 are a cause of severely impaired resistance to infection by M.tuberculosis. Ref.1
Sequence similarities	Contains 1 HSR domain. Contains 1 SAND domain.
Sequence caution	The sequence AAH18413.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Apoptosis Immunity Innate immunity Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Inferred from direct assay Ref.1. Source: MGI innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to bacterium Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 445

445

Sp110 nuclear body protein

PRO_0000247960

Regions

Domain

1 – 108

108

HSR

Domain

353 – 433

81

SAND

Motif

251 – 266

16

Nuclear localization signal Potential

Amino acid modifications

Modified residue

175

1

Phosphoserine Ref.4

Modified residue

177

1

Phosphoserine Ref.4

Modified residue

226

1

Phosphoserine By similarity

Experimental info

Sequence conflict

154

1

P → L in AAH18413. Ref.3

Sequence conflict

154

1

P → L in AAH39938. Ref.3

Sequence conflict

184

1

L → P in AAH18413. Ref.3

Sequence conflict

184

1

L → P in AAH39938. Ref.3

Sequence conflict

247

1

A → G in AAH39938. Ref.3

Sequence conflict

252

1

R → H in AAH39938. Ref.3

Sequence conflict

273

1

M → MA in AAH39938. Ref.3

Sequence conflict

310

1

Q → L in AAH39938. Ref.3

Sequence conflict

312

1

T → A in AAH39938. Ref.3

Sequence conflict

324

1

E → G in BAE29505. Ref.2

Sequence conflict

405

1

G → R in AAH39938. Ref.3

Sequence conflict

417

1

R → C in AAH39938. Ref.3

Sequence conflict

434 – 435

2

FT → CI in AAH39938. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

445

Helix Strand Turn

Details...

Helix

355 – 358

4

Beta strand

359 – 366

8

Beta strand

369 – 374

6

Helix

375 – 379

5

Helix

397 – 404

8

Helix

412 – 415

4

Helix

423 – 428

6

Sequences

	Sequence		Length	Mass (Da)
	Q8BVK9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A2F442C9ABFB3C5B	FASTA	445	50,140
	10 20 30 40 50 60 MFTLTKALEK ALLQHFIYMK VNIAYAINKP FPFFEALRDN SFITERMYKE SLEACQNLVP 70 80 90 100 110 120 LSKVVHNILT SLEQTFHPSV LLTLFSKVNL REYPSLVAIF RSFRNVGYTY EEKNRPPLTL 130 140 150 160 170 180 LEDLANPAEG CSLQTLLPPP RPQISLPSHL SSAPRVCDPR ATAQPIIEIL DEQPSPSPRA 190 200 210 220 230 240 VPLLGCIQEG KTTPVSSRDH QRKDKEDSRE MPHSPSGPES VVKDDSPAAN DLEMAREVPC 250 260 270 280 290 300 TPANKKARRK KRPNWSNSKR RRQKKKPRQD EMMGVASPGH GVQEKLKAVS RRTLWKDDSS 310 320 330 340 350 360 TNVKEVTKTQ RTRMRRAQTS NSQEISKEAS KTSGRKRPST ARRTTQVPEK TKNDAVDFSP 370 380 390 400 410 420 TLPVTCGKAK GTLFQEKLKQ GASKKCIQNE AGDWLTVKEF LNEGGRATSK DWKGVIRCNG 430 440 ETLRHLEQKG LLFFTSKSKP QKKGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ipr1 gene mediates innate immunity to tuberculosis." Pan H., Yan B.-S., Rojas M., Shebzukhov Y.V., Zhou H., Kobzik L., Higgins D.E., Daly M.J., Bloom B.R., Kramnik I. Nature 434:767-772(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISEASE, INDUCTION, TISSUE SPECIFICITY. Strain: C3Heb/FeJ. Tissue: Lung.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and NMRI. Tissue: Mammary tumor.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175 AND SER-177, MASS SPECTROMETRY. Tissue: Macrophage.
[5]	"Solution structure of the SAND domain of the putative nuclear protein homolog (5830484A20RIK)." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 353-433.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY845948 mRNA. Translation: AAW32543.1.
AK077800 mRNA. Translation: BAC37018.1.
AK150373 mRNA. Translation: BAE29505.1.
BC018413 mRNA. Translation: AAH18413.1. Sequence problems.
BC039938 mRNA. Translation: AAH39938.1.
BC094500 mRNA. Translation: AAH94500.1.

IPI

IPI00377843.

RefSeq

NP_084470.1. NM_030194.1.
NP_780606.3. NM_175397.4.

UniGene

Mm.335802.
Mm.461913.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UFN	NMR	-	A	353-433	[»]

ProteinModelPortal

Q8BVK9.

SMR

Q8BVK9. Positions 353-433.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000091226.

PTM databases

PhosphoSite

Q8BVK9.

Proteomic databases

PaxDb

Q8BVK9.

PRIDE

Q8BVK9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000093508; ENSMUSP00000091226; ENSMUSG00000070034.

GeneID

109032.

KEGG

mmu:109032.

UCSC

uc007btz.1. mouse.

Organism-specific databases

CTD

3431.

MGI

MGI:1923364. Sp110.

Phylogenomic databases

eggNOG

NOG306672.

GeneTree

ENSGT00510000046835.

HOGENOM

HOG000089984.

HOVERGEN

HBG006294.

InParanoid

Q8BVK9.

OMA

CARRCES.

OrthoDB

EOG4VDPZZ.

Gene expression databases

Bgee

Q8BVK9.

CleanEx

MM_SP110.

Genevestigator

Q8BVK9.

GermOnline

ENSMUSG00000070034. Mus musculus.

Family and domain databases

Gene3D

3.10.390.10. 1 hit.

InterPro

IPR000770. SAND_dom.
IPR010919. SAND_dom-like.
IPR004865. Sp100.
[Graphical view]

Pfam

PF01342. SAND. 1 hit.
PF03172. Sp100. 1 hit.
[Graphical view]

SMART

SM00258. SAND. 1 hit.
[Graphical view]

SUPFAM

SSF63763. SAND_like. 1 hit.

PROSITE

PS51414. HSR. 1 hit.
PS50864. SAND. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8BVK9.

NextBio

361588.

SOURCE

Search...

Entry information

Entry name

SP110_MOUSE

Accession

Primary (citable) accession number: Q8BVK9
Secondary accession number(s): Q05D41, Q3UCV7, Q80V00

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2006
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program